Gene Silencing Through CRISPR Interference in Bacteria: Current Advances and Future Prospects

On-target activity predictions enable improved CRISPR–dCas9 screens in bacteria

(2020) Alicia Calvo-Villamañán et al.   NUCLEIC ACIDS RESEARCH

Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes

(2020) Lun Yao et al.   Nature Communications

Genome-Wide CRISPRi-Based Identification of Targets for Decoupling Growth from Production

(2020) Songyuan Li et al.   ACS Synthetic Biology

Exploration of Bacterial Bottlenecks and Streptococcus pneumoniae Pathogenesis by CRISPRi-Seq

(2020) Xue Liu et al.   Cell Host & Microbe

Reversible Gene Expression Control in Yersinia pestis by Using an Optimized CRISPR Interference System

(2019) Tong Wang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Enhancing surfactin production by using systematic CRISPRi repression to screen amino acid biosynthesis genes in Bacillus subtilis

(2019) Congya Wang et al.   Microbial Cell Factories

Functional genomics of the rapidly replicating bacterium Vibrio natriegens by CRISPRi

(2019) Henry H. Lee et al.   Nature Microbiology

Analysis of lipoprotein transport depletion in Vibrio cholerae using CRISPRi

(2019) Florence Caro et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Modulating pathogenesis with Mobile-CRISPRi

(2019) Jiuxin Qu et al.   JOURNAL OF BACTERIOLOGY

Regulated Expression of sgRNAs Tunes CRISPRi in E. coli

(2018) Jason Fontana et al.   Biotechnology Journal

OUP accepted manuscript

(2018)   NUCLEIC ACIDS RESEARCH

A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9

(2018) Lun Cui et al.   Nature Communications

High-Level dCas9 Expression Induces Abnormal Cell Morphology in Escherichia coli

(2018) Suhyung Cho et al.   ACS Synthetic Biology

Regulated Expression of sgRNAs Tunes CRISPRi in E. coli

(2018) Jason Fontana et al.   Biotechnology Journal

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors

(2018) François Rousset et al.   PLoS Genetics

Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi

(2018) Jason M. Peters et al.   Nature Microbiology

CRISPR interference-guided multiplex repression of endogenous competing pathway genes for redirecting metabolic flux in Escherichia coli

(2017) Seong Keun Kim et al.   Microbial Cell Factories

CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production

(2017) Brady F. Cress et al.   Microbial Cell Factories

High‐throughput CRISPRi phenotyping identifies new essential genes in Streptococcus pneumoniae

(2017) Xue Liu et al.   Molecular Systems Biology

Time-Resolved Transposon Insertion Sequencing Reveals Genome-Wide Fitness Dynamics during Infection

(2017) Guanhua Yang et al.   mBio

Programmable transcriptional repression in mycobacteria using an orthogonal CRISPR interference platform

(2017) Jeremy M. Rock et al.   Nature Microbiology

Application of CRISPRi in Corynebacterium glutamicum for shikimic acid production

(2016) Bo Zhang et al.   BIOTECHNOLOGY LETTERS

A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria

(2016) Jason M. Peters et al.   CELL

tCRISPRi: tunable and reversible, one-step control of gene expression

(2016) Xin-tian Li et al.   Scientific Reports

Application of CRISPRi for prokaryotic metabolic engineering involving multiple genes, a case study: Controllable P(3HB- co -4HB) biosynthesis

(2015) Li Lv et al.   METABOLIC ENGINEERING

An updated evolutionary classification of CRISPR–Cas systems

(2015) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs

(2013) Dokyun Na et al.   NATURE BIOTECHNOLOGY

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

(2013) Seung Woo Cho et al.   NATURE BIOTECHNOLOGY

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system

(2013) David Bikard et al.   NUCLEIC ACIDS RESEARCH

crRNA and tracrRNA guide Cas9-mediated DNA interference inStreptococcus thermophilus

(2013) Tautvydas Karvelis et al.   RNA Biology

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

(2011) Elitza Deltcheva et al.   NATURE

Evolution and classification of the CRISPR–Cas systems

(2011) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Artificial trans -encoded small non-coding RNAs specifically silence the selected gene expression in bacteria

(2011) Shuai Man et al.   NUCLEIC ACIDS RESEARCH

Targeted Genome Editing Across Species Using ZFNs and TALENs

(2011) Andrew J. Wood et al.   SCIENCE

Tn-seq: high-throughput parallel sequencing for fitness and genetic interaction studies in microorganisms

(2009) Tim van Opijnen et al.   NATURE METHODS

Zinc-finger Nucleases: The Next Generation Emerges

(2008) Toni Cathomen et al.   MOLECULAR THERAPY

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE

CRISPR — a widespread system that provides acquired resistance against phages in bacteria and archaea

(2007) Rotem Sorek et al.   NATURE REVIEWS MICROBIOLOGY