Design of a programmable biosensor-CRISPRi genetic circuits for dynamic and autonomous dual-control of metabolic flux in Bacillus subtilis

Combinatorial pathway enzyme engineering and host engineering overcomes pyruvate overflow and enhances overproduction of N-acetylglucosamine in Bacillus subtilis

(2019) Wenlong Ma et al.   Microbial Cell Factories

CRISPR-assisted multi-dimensional regulation for fine-tuning gene expression in Bacillus subtilis

(2019) Zhenghui Lu et al.   NUCLEIC ACIDS RESEARCH

Complete biosynthesis of cannabinoids and their unnatural analogues in yeast

(2019) Xiaozhou Luo et al.   NATURE

Synthetic genetic circuits for programmable biological functionalities

(2019) Peng-Fei Xia et al.   BIOTECHNOLOGY ADVANCES

Kick-starting evolution efficiency with an autonomous evolution mutation system

(2019) Shihui Wang et al.   METABOLIC ENGINEERING

Overcoming genetic heterogeneity in industrial fermentations

(2019) Peter Rugbjerg et al.   NATURE BIOTECHNOLOGY

Synthetic N-terminal coding sequences for fine-tuning gene expression and metabolic engineering in Bacillus subtilis

(2019) Rongzhen Tian et al.   METABOLIC ENGINEERING

Custom-made transcriptional biosensors for metabolic engineering

(2019) Mathilde Koch et al.   CURRENT OPINION IN BIOTECHNOLOGY

Dynamic gene expression engineering as a tool in pathway engineering

(2019) Xiaolin Shen et al.   CURRENT OPINION IN BIOTECHNOLOGY

Metabolic engineering of carbon overflow metabolism of Bacillus subtilis for improved N-acetyl-glucosamine production

(2018) Wenlong Ma et al.   BIORESOURCE TECHNOLOGY

Development of N -acetylneuraminic acid responsive biosensors based on the transcriptional regulator NanR

(2018) Gert Peters et al.   BIOTECHNOLOGY AND BIOENGINEERING

Regulated Expression of sgRNAs Tunes CRISPRi in E. coli

(2018) Jason Fontana et al.   Biotechnology Journal

Production of chemicals using dynamic control of metabolic fluxes

(2018) Peng Xu   CURRENT OPINION IN BIOTECHNOLOGY

Current and future modalities of dynamic control in metabolic engineering

(2018) Makoto A Lalwani et al.   CURRENT OPINION IN BIOTECHNOLOGY

Layered dynamic regulation for improving metabolic pathway productivity inEscherichia coli

(2018) Stephanie J. Doong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High-Level dCas9 Expression Induces Abnormal Cell Morphology in Escherichia coli

(2018) Suhyung Cho et al.   ACS Synthetic Biology

Regulated Expression of sgRNAs Tunes CRISPRi in E. coli

(2018) Jason Fontana et al.   Biotechnology Journal

CRISPRi allows optimal temporal control of N-acetylglucosamine bioproduction by a dynamic coordination of glucose and xylose metabolism in Bacillus subtilis

(2018) Yaokang Wu et al.   METABOLIC ENGINEERING

Engineering a Glucosamine-6-phosphate Responsive glmS Ribozyme Switch Enables Dynamic Control of Metabolic Flux in Bacillus subtilis for Overproduction of N-Acetylglucosamine

(2018) Tengfei Niu et al.   ACS Synthetic Biology

A genetically encoded biosensor for monitoring isoprene production in engineered Escherichia coli

(2018) Seong Keun Kim et al.   ACS Synthetic Biology

Barriers and opportunities in bio-based production of hydrocarbons

(2018) Yongjin J. Zhou et al.   Nature Energy

Dynamic control of endogenous metabolism with combinatorial logic circuits

(2018) Felix Moser et al.   Molecular Systems Biology

Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors

(2018) Adam J. Meyer et al.   Nature Chemical Biology

Chimeric LysR-Type Transcriptional Biosensors for Customizing Ligand Specificity Profiles toward Flavonoids

(2018) Brecht De Paepe et al.   ACS Synthetic Biology

Reconstruction of a metabolic regulatory network in Escherichia coli for purposeful switching from cell growth mode to production mode in direct GABA fermentation from glucose

(2017) Yuki Soma et al.   METABOLIC ENGINEERING

Autonomous control of metabolic state by a quorum sensing (QS)-mediated regulator for bisabolene production in engineered E. coli

(2017) Eun-Mi Kim et al.   METABOLIC ENGINEERING

Dynamic regulation of metabolic flux in engineered bacteria using a pathway-independent quorum-sensing circuit

(2017) Apoorv Gupta et al.   NATURE BIOTECHNOLOGY

Digital logic circuits in yeast with CRISPR-dCas9 NOR gates

(2017) Miles W. Gander et al.   Nature Communications

Fundamental Design Principles for Transcription-Factor-Based Metabolite Biosensors

(2017) Ahmad A. Mannan et al.   ACS Synthetic Biology

Engineering Microbial Metabolite Dynamics and Heterogeneity

(2017) Alexander C. Schmitz et al.   Biotechnology Journal

Transcriptional regulation with CRISPR-Cas9: principles, advances, and applications

(2016) Andriy Didovyk et al.   CURRENT OPINION IN BIOTECHNOLOGY

Central metabolic nodes for diverse biochemical production

(2016) Lauren T Cordova et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

A part toolbox to tune genetic expression inBacillus subtilis

(2016) Sarah Guiziou et al.   NUCLEIC ACIDS RESEARCH

Enhancement of a high efficient autoinducible expression system in Bacillus subtilis by promoter engineering

(2016) Jintao Cheng et al.   PROTEIN EXPRESSION AND PURIFICATION

A dynamic pathway analysis approach reveals a limiting futile cycle in N-acetylglucosamine overproducing Bacillus subtilis

(2016) Yanfeng Liu et al.   Nature Communications

Development of GRAS strains for nutraceutical production using systems and synthetic biology approaches: advances and prospects

(2015) Long Liu et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Programmable genetic circuits for pathway engineering

(2015) Allison Hoynes-O’Connor et al.   CURRENT OPINION IN BIOTECHNOLOGY

Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways

(2015) Brady F Cress et al.   CURRENT OPINION IN BIOTECHNOLOGY

Allosteric control of transcription in GntR family of transcription regulators: A structural overview

(2015) Deepti Jain   IUBMB LIFE

Quorum-sensing linked RNA interference for dynamic metabolic pathway control in Saccharomyces cerevisiae

(2015) T.C. Williams et al.   METABOLIC ENGINEERING

Structural insight into operator dre-sites recognition and effector binding in the GntR/HutC transcription regulator NagR

(2015) Simon B. Fillenberg et al.   NUCLEIC ACIDS RESEARCH

Engineering a Lysine-ON Riboswitch for Metabolic Control of Lysine Production in Corynebacterium glutamicum

(2015) Li-Bang Zhou et al.   ACS Synthetic Biology

Comparison of genome-wide selection strategies to identify furfural tolerance genes in Escherichia coli

(2014) Tirzah Y. Glebes et al.   BIOTECHNOLOGY AND BIOENGINEERING

Synthesis of the Human Milk Oligosaccharide Lacto-N-Tetraose in Metabolically Engineered, Plasmid-FreeE. coli

(2014) Florian Baumgärtner et al.   CHEMBIOCHEM

Regulation of amino sugar utilization inBacillus subtilisby the GntR family regulators, NagR and GamR

(2014) Isabelle Gaugué et al.   MOLECULAR MICROBIOLOGY

Improving fatty acids production by engineering dynamic pathway regulation and metabolic control

(2014) P. Xu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

The Use of Amino Sugars by Bacillus subtilis: Presence of a Unique Operon for the Catabolism of Glucosamine

(2013) Isabelle Gaugué et al.   PLoS One

Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids

(2012) Fuzhong Zhang et al.   NATURE BIOTECHNOLOGY

Engineering Static and Dynamic Control of Synthetic Pathways

(2010) William J. Holtz et al.   CELL

An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol

(2009) Huanting Liu et al.   BMC BIOTECHNOLOGY

Cre/lox System and PCR-Based Genome Engineering in Bacillus subtilis

(2008) X. Yan et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY