Synthetic biomolecular condensates to engineer eukaryotic cells

Artificial organelles: towards adding or restoring intracellular activity

(2021) Roy A.J.F. Oerlemans et al.   CHEMBIOCHEM

Designer Condensates: A Toolkit for the Biomolecular Architect

(2021) Renee L. Hastings et al.   JOURNAL OF MOLECULAR BIOLOGY

Synthetic Protein Condensates That Inducibly Recruit and Release Protein Activity in Living Cells

(2021) Masaru Yoshikawa et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Dual film-like organelles enable spatial separation of orthogonal eukaryotic translation

(2021) Christopher D. Reinkemeier et al.   CELL

Physical Principles Underlying the Complex Biology of Intracellular Phase Transitions

(2020) Jeong-Mo Choi et al.   Annual Review of Biophysics

SPLIT: Stable Protein Coacervation Using a Light Induced Transition

(2020) Ellen H. Reed et al.   ACS Synthetic Biology

Selective sequestration of signalling proteins in a membraneless organelle reinforces the spatial regulation of asymmetry in Caulobacter crescentus

(2020) Keren Lasker et al.   Nature Microbiology

Rapid discovery and evolution of orthogonal aminoacyl-tRNA synthetase–tRNA pairs

(2020) Daniele Cervettini et al.   NATURE BIOTECHNOLOGY

Engineered triply orthogonal pyrrolysyl–tRNA synthetase/tRNA pairs enable the genetic encoding of three distinct non-canonical amino acids

(2020) Daniel L. Dunkelmann et al.   Nature Chemistry

Raising the ribosomal repertoire

(2020) Christopher D. Reinkemeier et al.   Nature Chemistry

Designer protein assemblies with tunable phase diagrams in living cells

(2020) Meta Heidenreich et al.   Nature Chemical Biology

De novo engineering of intracellular condensates using artificial disordered proteins

(2020) Michael Dzuricky et al.   Nature Chemistry

Nucleated transcriptional condensates amplify gene expression

(2020) Ming-Tzo Wei et al.   NATURE CELL BIOLOGY

Tunable multiphase dynamics of arginine and lysine liquid condensates

(2020) Rachel S. Fisher et al.   Nature Communications

Reprogramming the genetic code

(2020) Daniel de la Torre et al.   NATURE REVIEWS GENETICS

Designer membraneless organelles enable codon reassignment of selected mRNAs in eukaryotes

(2019) Christopher D. Reinkemeier et al.   SCIENCE

Regulation of Transmembrane Signaling by Phase Separation

(2019) Lindsay B. Case et al.   Annual Review of Biophysics

Total synthesis of Escherichia coli with a recoded genome

(2019) Julius Fredens et al.   NATURE

Light-based control of metabolic flux through assembly of synthetic organelles

(2019) Evan M. Zhao et al.   Nature Chemical Biology

Innovation by Evolution: Bringing New Chemistry to Life (Nobel Lecture)

(2019) Frances H. Arnold   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Progress toward Eukaryotic Semisynthetic Organisms: Translation of Unnatural Codons

(2019) Anne Xiao-Zhou Zhou et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Probing and engineering liquid-phase organelles

(2019) Dan Bracha et al.   NATURE BIOTECHNOLOGY

Visualizing Dynamics of Cell Signaling In Vivo with a Phase Separation-Based Kinase Reporter

(2018) Qiang Zhang et al.   MOLECULAR CELL

Protein Phase Separation Provides Long-Term Memory of Transient Spatial Stimuli

(2018) Elliot Dine et al.   Cell Systems

Controllable protein phase separation and modular recruitment to form responsive membraneless organelles

(2018) Benjamin S. Schuster et al.   Nature Communications

Dynamic Imaging of Small Molecule Induced Protein–Protein Interactions in Living Cells with a Fluorophore Phase Transition Based Approach

(2018) Chan-I Chung et al.   ANALYTICAL CHEMISTRY

Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome

(2018) Yongdae Shin et al.   CELL

Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds

(2018) Dan Bracha et al.   CELL

Controlling orthogonal ribosome subunit interactions enables evolution of new function

(2018) Wolfgang H. Schmied et al.   NATURE

Expanding and reprogramming the genetic code

(2017) Jason W. Chin   NATURE

A semi-synthetic organism that stores and retrieves increased genetic information

(2017) Yorke Zhang et al.   NATURE

Programming molecular self-assembly of intrinsically disordered proteins containing sequences of low complexity

(2017) Joseph R. Simon et al.   Nature Chemistry

Intracellular production of hydrogels and synthetic RNA granules by multivalent molecular interactions

(2017) Hideki Nakamura et al.   NATURE MATERIALS

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Genetic visualization of protein interactions harnessing liquid phase transitions

(2017) Taku Watanabe et al.   Scientific Reports

Debugging Eukaryotic Genetic Code Expansion for Site-Specific Click-PAINT Super-Resolution Microscopy

(2016) Ivana Nikić et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Are aberrant phase transitions a driver of cellular aging?

(2016) Simon Alberti et al.   BIOESSAYS

Compositional Control of Phase-Separated Cellular Bodies

(2016) Salman F. Banani et al.   CELL

The coming of age of de novo protein design

(2016) Po-Ssu Huang et al.   NATURE

Genetic Code Expansion Enables Live-Cell and Super-Resolution Imaging of Site-Specifically Labeled Cellular Proteins

(2015) Chayasith Uttamapinant et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Protein synthesis by ribosomes with tethered subunits

(2015) Cédric Orelle et al.   NATURE

Intrinsically disordered proteins in cellular signalling and regulation

(2015) Peter E. Wright et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

RNA transcription modulates phase transition-driven nuclear body assembly

(2015) Joel Berry et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Minimal Tags for Rapid Dual-Color Live-Cell Labeling and Super-Resolution Microscopy

(2014) Ivana Nikić et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Intrinsically Disordered Proteins and Intrinsically Disordered Protein Regions

(2014) Christopher J. Oldfield et al.   Annual Review of Biochemistry

Pyrrolysyl-tRNA synthetase: An ordinary enzyme but an outstanding genetic code expansion tool

(2014) Wei Wan et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Phase transitions of multivalent proteins can promote clustering of membrane receptors

(2014) Sudeep Banjade et al.   eLife

Genomically Recoded Organisms Expand Biological Functions

(2013) M. J. Lajoie et al.   SCIENCE

Phase transitions in the assembly of multivalent signalling proteins

(2012) Pilong Li et al.   NATURE

Adding New Chemistries to the Genetic Code

(2010) Chang C. Liu et al.   Annual Review of Biochemistry

Genetically Encoded Photocontrol of Protein Localization in Mammalian Cells

(2010) Arnaud Gautier et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation

(2009) C. P. Brangwynne et al.   SCIENCE