Expanding and reprogramming the genetic code

An orthogonalized platform for genetic code expansion in both bacteria and eukaryotes

(2017) James S Italia et al.   Nature Chemical Biology

Genetically encoding phosphotyrosine and its nonhydrolyzable analog in bacteria

(2017) Xiaozhou Luo et al.   Nature Chemical Biology

Site-specific incorporation of phosphotyrosine using an expanded genetic code

(2017) Christian Hoppmann et al.   Nature Chemical Biology

Biosynthesis and genetic encoding of phosphothreonine through parallel selection and deep sequencing

(2017) Michael Shaofei Zhang et al.   NATURE METHODS

Large-scale recoding of a bacterial genome by iterative recombineering of synthetic DNA

(2017) Yu Heng Lau et al.   NUCLEIC ACIDS RESEARCH

A semisynthetic organism engineered for the stable expansion of the genetic alphabet

(2017) Yorke Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Design of a synthetic yeast genome

(2017) Sarah M. Richardson et al.   SCIENCE

Expanding the genetic code of Mus musculus

(2017) Songmi Han et al.   Nature Communications

A Genetically Encoded Allysine for the Synthesis of Proteins with Site-Specific Lysine Dimethylation

(2016) Zhipeng A. Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Expanding the genetic code ofEscherichia coliwith phosphotyrosine

(2016) Chenguang Fan et al.   FEBS LETTERS

A General Strategy for the Semisynthesis of Ratiometric Fluorescent Sensor Proteins with Increased Dynamic Range

(2016) Lin Xue et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Photoactivation of Mutant Isocitrate Dehydrogenase 2 Reveals Rapid Cancer-Associated Metabolic and Epigenetic Changes

(2016) Olivia S. Walker et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Palladium-Triggered Chemical Rescue of Intracellular Proteins via Genetically Encoded Allene-Caged Tyrosine

(2016) Jie Wang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

In Vivo Biosynthesis of a β-Amino Acid-Containing Protein

(2016) Clarissa Melo Czekster et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Site-Specific Bioorthogonal Labeling for Fluorescence Imaging of Intracellular Proteins in Living Cells

(2016) Tao Peng et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Performance of optimized noncanonical amino acid mutagenesis systems in the absence of release factor 1

(2016) Yunan Zheng et al.   Molecular BioSystems

Defining synonymous codon compression schemes by genome recoding

(2016) Kaihang Wang et al.   NATURE

Genetic code expansion in the mouse brain

(2016) Russell J Ernst et al.   Nature Chemical Biology

Addicting diverse bacteria to a noncanonical amino acid

(2016) Drew S Tack et al.   Nature Chemical Biology

Expanding the amino acid repertoire of ribosomal polypeptide synthesis via the artificial division of codon boxes

(2016) Yoshihiko Iwane et al.   Nature Chemistry

Expanding the chemical toolbox for the synthesis of large and uniquely modified proteins

(2016) Somasekhar Bondalapati et al.   Nature Chemistry

Genetic code expansion in stable cell lines enables encoded chromatin modification

(2016) Simon J Elsässer et al.   NATURE METHODS

Emergent rules for codon choice elucidated by editing rare arginine codons inEscherichia coli

(2016) Michael G. Napolitano et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Computational design of a homotrimeric metalloprotein with a trisbipyridyl core

(2016) Jeremy H. Mills et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Versatile strategy for controlling the specificity and activity of engineered T cells

(2016) Jennifer S. Y. Ma et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Design, synthesis, and testing toward a 57-codon genome

(2016) N. Ostrov et al.   SCIENCE

A chemical biology route to site-specific authentic protein modifications

(2016) A. Yang et al.   SCIENCE

Posttranslational mutagenesis: A chemical strategy for exploring protein side-chain diversity

(2016) T. H. Wright et al.   SCIENCE

Generation of influenza A viruses as live but replication-incompetent virus vaccines

(2016) L. Si et al.   SCIENCE

Genetically encoded protein photocrosslinker with a transferable mass spectrometry-identifiable label

(2016) Yi Yang et al.   Nature Communications

Virus-Enabled Optimization and Delivery of the Genetic Machinery for Efficient Unnatural Amino Acid Mutagenesis in Mammalian Cells and Tissues

(2016) Yunan Zheng et al.   ACS Synthetic Biology

Application of Noncanonical Amino Acids for Protein Labeling in a Genomically Recoded Escherichia coli

(2016) Kalle Kipper et al.   ACS Synthetic Biology

Bioorthogonal Chemical Activation of Kinases in Living Systems

(2016) Gong Zhang et al.   ACS Central Science

Ribosome Subunit Stapling for Orthogonal Translation inE. coli

(2015) Stephen D. Fried et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Protein Synthesis with Ribosomes Selected for the Incorporation of β-Amino Acids

(2015) Rumit Maini et al.   BIOCHEMISTRY

Genetically Encoded Azide Containing Amino Acid in Mammalian Cells Enables Site-Specific Antibody–Drug Conjugates Using Click Cycloaddition Chemistry

(2015) Michael P. VanBrunt et al.   BIOCONJUGATE CHEMISTRY

Ribosome-Mediated Incorporation of Dipeptides and Dipeptide Analogues into Proteins in Vitro

(2015) Rumit Maini et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genetic Code Expansion Enables Live-Cell and Super-Resolution Imaging of Site-Specifically Labeled Cellular Proteins

(2015) Chayasith Uttamapinant et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

In Situ Formation of an Azo Bridge on Proteins Controllable by Visible Light

(2015) Christian Hoppmann et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Optical Control of CRISPR/Cas9 Gene Editing

(2015) James Hemphill et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Protein synthesis by ribosomes with tethered subunits

(2015) Cédric Orelle et al.   NATURE

Biocontainment of genetically modified organisms by synthetic protein design

(2015) Daniel J. Mandell et al.   NATURE

Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids

(2015) Miriam Amiram et al.   NATURE BIOTECHNOLOGY

Efficient genetic encoding of phosphoserine and its nonhydrolyzable analog

(2015) Daniel T Rogerson et al.   Nature Chemical Biology

Selective, rapid and optically switchable regulation of protein function in live mammalian cells

(2015) Yu-Hsuan Tsai et al.   Nature Chemistry

Exploring the potential impact of an expanded genetic code on protein function

(2015) Han Xiao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The ribosome can discriminate the chirality of amino acids within its peptidyl-transferase center

(2015) Michael T. Englander et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Trapping a transition state in a computationally designed protein bottle

(2015) A. D. Pearson et al.   SCIENCE

Designing logical codon reassignment – Expanding the chemistry in biology

(2015) Anaëlle Dumas et al.   Chemical Science

Highly reproductive Escherichia coli cells with no specific assignment to the UAG codon

(2015) Takahito Mukai et al.   Scientific Reports

Synthetic Auxotrophs with Ligand-Dependent Essential Genes for a BL21(DE3) Biosafety Strain

(2015) Gabriel Lopez et al.   ACS Synthetic Biology

Protein Sectors: Statistical Coupling Analysis versus Conservation

(2015) Tiberiu Teşileanu et al.   PLoS Computational Biology

EGF-dependent re-routing of vesicular recycling switches spontaneous phosphorylation suppression to EGFR signaling

(2015) Martin Baumdick et al.   eLife

Optical Control of Protein Function through Unnatural Amino Acid Mutagenesis and Other Optogenetic Approaches

(2014) Austin S. Baker et al.   ACS Chemical Biology

Bioorthogonal Reactions for Labeling Proteins

(2014) Kathrin Lang et al.   ACS Chemical Biology

Construction of a Live-Attenuated HIV-1 Vaccine through Genetic Code Expansion

(2014) Nanxi Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Expanding and Reprogramming the Genetic Code of Cells and Animals

(2014) Jason W. Chin   Annual Review of Biochemistry

Gleaning Unexpected Fruits from Hard-Won Synthetases: Probing Principles of Permissivity in Non-canonical Amino Acid-tRNA Synthetases

(2014) Richard B. Cooley et al.   CHEMBIOCHEM

In Vivo Incorporation of Non-canonical Amino Acids by Using the Chemical Aminoacylation Strategy: A Broadly Applicable Mechanistic Tool

(2014) Dennis A. Dougherty et al.   CHEMBIOCHEM

A Bacterial Strain with a Unique Quadruplet Codon Specifying Non-native Amino Acids

(2014) Abhishek Chatterjee et al.   CHEMBIOCHEM

Towards Reassigning the Rare AGG Codon inEscherichia coli

(2014) Yu Zeng et al.   CHEMBIOCHEM

Cellular Incorporation of Unnatural Amino Acids and Bioorthogonal Labeling of Proteins

(2014) Kathrin Lang et al.   CHEMICAL REVIEWS

Genetic Encoding of Photocaged Cysteine Allows Photoactivation of TEV Protease in Live Mammalian Cells

(2014) Duy P. Nguyen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Concerted, Rapid, Quantitative, and Site-Specific Dual Labeling of Proteins

(2014) Amit Sachdeva et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genetically Encoded Optochemical Probes for Simultaneous Fluorescence Reporting and Light Activation of Protein Function with Two-Photon Excitation

(2014) Ji Luo et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Efficient Multisite Unnatural Amino Acid Incorporation in Mammalian Cells via Optimized Pyrrolysyl tRNA Synthetase/tRNA Expression and Engineered eRF1

(2014) Wolfgang H. Schmied et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A semi-synthetic organism with an expanded genetic alphabet

(2014) Denis A. Malyshev et al.   NATURE

Proteome labeling and protein identification in specific tissues and at specific developmental stages in an animal

(2014) Thomas S Elliott et al.   NATURE BIOTECHNOLOGY

Bacteriophages use an expanded genetic code on evolutionary paths to higher fitness

(2014) Michael J Hammerling et al.   Nature Chemical Biology

Diels-Alder reaction–triggered bioorthogonal protein decaging in living cells

(2014) Jie Li et al.   Nature Chemical Biology

An orthogonal ribosome-tRNA pair via engineering of the peptidyl transferase center

(2014) Naohiro Terasaka et al.   Nature Chemical Biology

Palladium-triggered deprotection chemistry for protein activation in living cells

(2014) Jie Li et al.   Nature Chemistry

Optimized orthogonal translation of unnatural amino acids enables spontaneous protein double-labelling and FRET

(2014) Kaihang Wang et al.   Nature Chemistry

Evidence of efficient stop codon readthrough in four mammalian genes

(2014) Gary Loughran et al.   NUCLEIC ACIDS RESEARCH

A general approach to site-specific antibody drug conjugates

(2014) Feng Tian et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Recruiting Cytotoxic T Cells to Folate-Receptor-Positive Cancer Cells

(2013) Sumith A. Kularatne et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Site-Specific Engineering of Chemical Functionalities on the Surface of Live Hepatitis D Virus

(2013) Shixian Lin et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Genetic Incorporation of Multiple Unnatural Amino Acids into Proteins in Mammalian Cells

(2013) Han Xiao et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Versatile Platform for Single- and Multiple-Unnatural Amino Acid Mutagenesis in Escherichia coli

(2013) Abhishek Chatterjee et al.   BIOCHEMISTRY

Genetically Encoded Chemical Probes in Cells Reveal the Binding Path of Urocortin-I to CRF Class B GPCR

(2013) Irene Coin et al.   CELL

Multiple Site-Selective Insertions of Noncanonical Amino Acids into Sequence-Repetitive Polypeptides

(2013) I-Lin Wu et al.   CHEMBIOCHEM

Computational Design of an Unnatural Amino Acid Dependent Metalloprotein with Atomic Level Accuracy

(2013) Jeremy H. Mills et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Reevaluation of the d-Amino Acid Compatibility with the Elongation Event in Translation

(2013) Tomoshige Fujino et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Genetically Encoded Fluorescent Probe in Mammalian Cells

(2013) Abhishek Chatterjee et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Directed evolution of genetic parts and circuits by compartmentalized partnered replication

(2013) Jared W Ellefson et al.   NATURE BIOTECHNOLOGY

A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins

(2013) Gražvydas Lukinavičius et al.   Nature Chemistry

Genomically Recoded Organisms Expand Biological Functions

(2013) M. J. Lajoie et al.   SCIENCE

Evolution of multiple, mutually orthogonal prolyl-tRNA synthetase/tRNA pairs for unnatural amino acid mutagenesis in Escherichia coli

(2012) A. Chatterjee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Noncanonical Amino Acids in the Interrogation of Cellular Protein Synthesis

(2011) John T. Ngo et al.   ACCOUNTS OF CHEMICAL RESEARCH

An Evolved Aminoacyl-tRNA Synthetase with Atypical Polysubstrate Specificity,

(2011) Douglas D. Young et al.   BIOCHEMISTRY

Kinetics of Radical Intermediate Formation and Deoxynucleotide Production in 3-Aminotyrosine-SubstitutedEscherichia coliRibonucleotide Reductases

(2011) Ellen C. Minnihan et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites

(2011) David B F Johnson et al.   Nature Chemical Biology

Expanding the Genetic Code of Escherichia coli with Phosphoserine

(2011) H.-S. Park et al.   SCIENCE

Adding New Chemistries to the Genetic Code

(2010) Chang C. Liu et al.   Annual Review of Biochemistry

De NovoGeneration of Mutually Orthogonal Aminoacyl-tRNA Synthetase/tRNA Pairs

(2010) Heinz Neumann et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome

(2010) Heinz Neumann et al.   NATURE

Codon reassignment in the Escherichia coli genetic code

(2010) T. Mukai et al.   NUCLEIC ACIDS RESEARCH

Rational design of an orthogonal tryptophanyl nonsense suppressor tRNA

(2010) Randall A. Hughes et al.   NUCLEIC ACIDS RESEARCH

Functional replacement of the endogenous tyrosyl-tRNA synthetase–tRNATyr pair by the archaeal tyrosine pair in Escherichia coli for genetic code expansion

(2010) Fumie Iraha et al.   NUCLEIC ACIDS RESEARCH

Recognition of Non-α-amino Substrates by Pyrrolysyl-tRNA Synthetase

(2008) Takatsugu Kobayashi et al.   JOURNAL OF MOLECULAR BIOLOGY

Genetically encoding Nε-acetyllysine in recombinant proteins

(2008) Heinz Neumann et al.   Nature Chemical Biology

Addition of an α-Hydroxy Acid to the Genetic Code of Bacteria

(2007) Jiantao Guo et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION