Programming Cells by Multicopy Chromosomal Integration Using CRISPR-Associated Transposases

Dual modes of CRISPR-associated transposon homing

(2021) Makoto Saito et al.   CELL

CRISPRi-Based Dynamic Control of Carbon Flow for Efficient N-Acetyl Glucosamine Production and Its Metabolomic Effects in Escherichia coli

(2020) Quanwei Zhang et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

A high-throughput screening assay for the directed evolution-guided discovery of halohydrin dehalogenase mutants for epoxide ring-opening reaction

(2020) Ijaz Gul et al.   JOURNAL OF BIOTECHNOLOGY

Response to Comment on “RNA-guided DNA insertion with CRISPR-associated transposases”

(2020) Jonathan Strecker et al.   SCIENCE

Degeneration of industrial bacteria caused by genetic instability

(2020) Mengxue Peng et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Multicopy Chromosomal Integration Using CRISPR-Associated Transposases

(2020) Yiwen Zhang et al.   ACS Synthetic Biology

Guide RNA Categorization Enables Target Site Choice in Tn7-CRISPR-Cas Transposons

(2020) Michael T. Petassi et al.   CELL

CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering

(2020) Phuc Leo H. Vo et al.   NATURE BIOTECHNOLOGY

A fast and robust iterative genome-editing method based on a Rock-Paper-Scissors strategy

(2020) Jichao Wang et al.   NUCLEIC ACIDS RESEARCH

Barriers to genome editing with CRISPR in bacteria

(2019) Justin M. Vento et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration

(2019) Sanne E. Klompe et al.   NATURE

RNA-guided DNA insertion with CRISPR-associated transposases

(2019) Jonathan Strecker et al.   SCIENCE

Synthetic Genomics: From DNA Synthesis to Genome Design

(2018) Lianrong Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Targeted Nucleotide Editing Technologies for Microbial Metabolic Engineering

(2018) Takayuki Arazoe et al.   Biotechnology Journal

Continuous directed evolution for strain and protein engineering

(2018) Simon d’Oelsnitz et al.   CURRENT OPINION IN BIOTECHNOLOGY

The CRISPR tool kit for genome editing and beyond

(2018) Mazhar Adli   Nature Communications

Targeted Nucleotide Editing Technologies for Microbial Metabolic Engineering

(2018) Takayuki Arazoe et al.   Biotechnology Journal

DNA sequencing at 40: past, present and future

(2017) Jay Shendure et al.   NATURE

Recruitment of CRISPR-Cas systems by Tn7-like transposons

(2017) Joseph E. Peters et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Screening for improved isoprenoid biosynthesis in microorganisms

(2016) Anita Emmerstorfer-Augustin et al.   JOURNAL OF BIOTECHNOLOGY

Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System

(2015) Yu Jiang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Directed evolution of the peroxidase activity of a de novo-designed protein

(2012) S. C. Patel et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Application of the bacteriophage Mu-driven system for the integration/amplification of target genes in the chromosomes of engineered Gram-negative bacteria—mini review

(2011) Valerii Z. Akhverdyan et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Plasmid addiction systems: perspectives and applications in biotechnology

(2010) Jens Kroll et al.   Microbial Biotechnology

Ultrahigh-Throughput FACS-Based Screening for Directed Enzyme Evolution

(2009) Guangyu Yang et al.   CHEMBIOCHEM

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE