A fast and robust iterative genome-editing method based on a Rock-Paper-Scissors strategy

Metabolic Engineering of Escherichia coli for Natural Product Biosynthesis

(2020) Dongsoo Yang et al.   TRENDS IN BIOTECHNOLOGY

Synthetic genetic circuits for programmable biological functionalities

(2019) Peng-Fei Xia et al.   BIOTECHNOLOGY ADVANCES

Escherichia coli vectors having stringently repressible replication origins allow a streamlining of Crispr/Cas9 gene editing

(2019) Swaminath Srinivas et al.   PLASMID

Rock-paper-scissors: Engineered population dynamics increase genetic stability

(2019) Michael J. Liao et al.   SCIENCE

CRISPR-Cas9-assisted native end-joining editing offers a simple strategy for efficient genetic engineering in Escherichia coli

(2019) Chaoyong Huang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Escherichia coli as a host for metabolic engineering

(2018) Sammy Pontrelli et al.   METABOLIC ENGINEERING

A Multiplex Genome Editing Method for Escherichia coli Based on CRISPR-Cas12a

(2018) Xiang Ao et al.   Frontiers in Microbiology

Large scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli

(2017) Francesca Zerbini et al.   Microbial Cell Factories

An electroporation-free method based on Red recombineering for markerless deletion and genomic replacement in the Escherichia coli DH1 genome

(2017) Yanlong Wei et al.   PLoS One

CRISPR/Cas9 in Genome Editing and Beyond

(2016) Haifeng Wang et al.   Annual Review of Biochemistry

CRISPR technologies for bacterial systems: Current achievements and future directions

(2016) Kyeong Rok Choi et al.   BIOTECHNOLOGY ADVANCES

Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9

(2016) Dongdong Zhao et al.   Microbial Cell Factories

A CRISPR-Cas9 Assisted Non-Homologous End-Joining Strategy for One-step Engineering of Bacterial Genome

(2016) Tianyuan Su et al.   Scientific Reports

Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli

(2015) Michael E. Pyne et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System

(2015) Yu Jiang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Metabolic engineering of Escherichia coli using CRISPR–Cas9 meditated genome editing

(2015) Yifan Li et al.   METABOLIC ENGINEERING

Precision-engineering the Pseudomonas aeruginosa genome with two-step allelic exchange

(2015) Laura R Hmelo et al.   Nature Protocols

Seven gene deletions in seven days: Fast generation of Escherichia coli strains tolerant to acetate and osmotic stress

(2015) Sheila I. Jensen et al.   Scientific Reports

High-Efficiency Scarless Genetic Modification in Escherichia coli by Using Lambda Red Recombination and I-SceI Cleavage

(2014) Junjie Yang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Production of optically pure d -lactate from glycerol by engineered Klebsiella pneumoniae strain

(2014) Xinjun Feng et al.   BIORESOURCE TECHNOLOGY

sgRNAcas9: A Software Package for Designing CRISPR sgRNA and Evaluating Potential Off-Target Cleavage Sites

(2014) Shengsong Xie et al.   PLoS One

PR-PR: Cross-Platform Laboratory Automation System

(2014) Gregory Linshiz et al.   ACS Synthetic Biology

Genome replication engineering assisted continuous evolution (GREACE) to improve microbial tolerance for biofuels production

(2013) Guodong Luan et al.   Biotechnology for Biofuels

Rapid one-step inactivation of single or multiple genes inEscherichia coli

(2013) Chan Woo Song et al.   Biotechnology Journal

Genome-scale engineering for systems and synthetic biology

(2013) K. M. Esvelt et al.   Molecular Systems Biology

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

PaR-PaR Laboratory Automation Platform

(2012) Gregory Linshiz et al.   ACS Synthetic Biology

Update on the Keio collection of Escherichia coli single-gene deletion mutants

(2009) Natsuko Yamamoto et al.   Molecular Systems Biology

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Tn-seq: high-throughput parallel sequencing for fitness and genetic interaction studies in microorganisms

(2009) Tim van Opijnen et al.   NATURE METHODS