Streamlined Human Cell-Based Recombinase-Mediated Cassette Exchange Platform Enables Multigene Expression for the Production of Therapeutic Proteins
Published 2021 View Full Article
- Home
- Publications
- Publication Search
- Publication Details
Title
Streamlined Human Cell-Based Recombinase-Mediated Cassette Exchange Platform Enables Multigene Expression for the Production of Therapeutic Proteins
Authors
Keywords
-
Journal
ACS Synthetic Biology
Volume 10, Issue 7, Pages 1715-1727
Publisher
American Chemical Society (ACS)
Online
2021-06-17
DOI
10.1021/acssynbio.1c00113
References
Ask authors/readers for more resources
Related references
Note: Only part of the references are listed.- Development of a Targeted Integration Chinese Hamster Ovary ( CHO ) Host Directly Targeting Either One or Two Vectors Simultaneously to a Single Locus Using the Cre/Lox RMCE System
- (2021) Domingos Ng et al. BIOTECHNOLOGY PROGRESS
- Maximizing antibody production in a targeted integration host by optimization of subunit gene dosage and position
- (2020) Joe Carver et al. BIOTECHNOLOGY PROGRESS
- The use of site-specific recombination and cassette exchange technologies for monoclonal antibody production in Chinese Hamster ovary cells: retrospective analysis and future directions
- (2020) Kajan Srirangan et al. CRITICAL REVIEWS IN BIOTECHNOLOGY
- Comprehensive Analysis of Genomic Safe Harbors as Target Sites for Stable Expression of the Heterologous Gene in HEK293 Cells
- (2020) Seunghyeon Shin et al. ACS Synthetic Biology
- Genetic switches designed for eukaryotic cells and controlled by serine integrases
- (2020) Mayna S. Gomide et al. Communications Biology
- High throughput generation of a resource of the human secretome in mammalian cells
- (2020) Hanna Tegel et al. New Biotechnology
- A double-Flp-in method for stable overexpression of two genes
- (2020) Ole Jensen et al. Scientific Reports
- Multicopy Targeted Integration for Accelerated Development of High-Producing Chinese Hamster Ovary Cells
- (2020) Daria Sergeeva et al. ACS Synthetic Biology
- Systematic Evaluation of Site-Specific Recombinant Gene Expression for Programmable Mammalian Cell Engineering
- (2019) Nuša Pristovšek et al. ACS Synthetic Biology
- Mitigating Clonal Variation in Recombinant Mammalian Cell Lines
- (2019) Jae Seong Lee et al. TRENDS IN BIOTECHNOLOGY
- Enhanced Cas12a editing in mammalian cells and zebrafish
- (2019) Pengpeng Liu et al. NUCLEIC ACIDS RESEARCH
- Small-molecule control of antibody N-glycosylation in engineered mammalian cells
- (2019) Michelle M. Chang et al. Nature Chemical Biology
- A system for site-specific integration of transgenes in mammalian cells
- (2019) Xiuling Chi et al. PLoS One
- An improved platform for functional assessment of large protein libraries in mammalian cells
- (2019) Kenneth A Matreyek et al. NUCLEIC ACIDS RESEARCH
- A human expression system based on HEK293 for the stable production of recombinant erythropoietin
- (2019) Christine Lin Chin et al. Scientific Reports
- A multi-landing pad DNA integration platform for mammalian cell engineering
- (2018) Leonid Gaidukov et al. NUCLEIC ACIDS RESEARCH
- Comprehensive characterization of glutamine synthetase-mediated selection for the establishment of recombinant CHO cells producing monoclonal antibodies
- (2018) Soo Min Noh et al. Scientific Reports
- Minimizing Clonal Variation during Mammalian Cell Line Engineering for Improved Systems Biology Data Generation
- (2018) Lise Marie Grav et al. ACS Synthetic Biology
- Revealing Key Determinants of Clonal Variation in Transgene Expression in Recombinant CHO Cells Using Targeted Genome Editing
- (2018) Jae Seong Lee et al. ACS Synthetic Biology
- Site-specific integration ushers in a new era of precise CHO cell line engineering
- (2018) Nathaniel K Hamaker et al. Current Opinion in Chemical Engineering
- In silico design of context-responsive mammalian promoters with user-defined functionality
- (2017) Adam J. Brown et al. NUCLEIC ACIDS RESEARCH
- A platform for functional assessment of large variant libraries in mammalian cells
- (2017) Kenneth A. Matreyek et al. NUCLEIC ACIDS RESEARCH
- Site-specific chromosomal gene insertion: Flp recombinase versus Cas9 nuclease
- (2017) Quang Vinh Phan et al. Scientific Reports
- Engineered Bispecific Antibodies with Exquisite HIV-1-Neutralizing Activity
- (2016) Yaoxing Huang et al. CELL
- Overexpression of YY1 increases the protein production in mammalian cells
- (2016) Aizhan Tastanova et al. JOURNAL OF BIOTECHNOLOGY
- A method for specifically targeting two independent genomic integration sites for co-expression of genes in CHO cells
- (2016) Bahar Baser et al. METHODS
- In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration
- (2016) Keiichiro Suzuki et al. NATURE
- Integrated cell and process engineering for improved transient production of a “difficult-to-express“ fusion protein by CHO cells
- (2015) Yusuf B. Johari et al. BIOTECHNOLOGY AND BIOENGINEERING
- CRISPR/Cas9-mediated genome engineering of CHO cell factories: Application and perspectives
- (2015) Jae Seong Lee et al. Biotechnology Journal
- Recombinase-mediated cassette exchange (RMCE) for monoclonal antibody expression in the commercially relevant CHOK1SV cell line
- (2015) Lin Zhang et al. BIOTECHNOLOGY PROGRESS
- Human cell lines for biopharmaceutical manufacturing: history, status, and future perspectives
- (2015) Jennifer Dumont et al. CRITICAL REVIEWS IN BIOTECHNOLOGY
- Target-specific variants of Flp recombinase mediate genome engineering reactions in mammalian cells
- (2015) Riddhi Shah et al. FEBS Journal
- Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells
- (2015) Van Trung Chu et al. NATURE BIOTECHNOLOGY
- Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway
- (2015) Jae Seong Lee et al. Scientific Reports
- CRISPR/Cas9-mediated genome engineering of CHO cell factories: Application and perspectives
- (2015) Jae Seong Lee et al. Biotechnology Journal
- Combating non-Hodgkin lymphoma by targeting both CD20 and HLA-DR through CD20–243 CrossMab
- (2014) Lei Zhao et al. mAbs
- A platform for rapid prototyping of synthetic gene networks in mammalian cells
- (2014) Xavier Duportet et al. NUCLEIC ACIDS RESEARCH
- Model-directed engineering of “difficult-to-express” monoclonal antibody production by Chinese hamster ovary cells
- (2013) Leon P. Pybus et al. BIOTECHNOLOGY AND BIOENGINEERING
- Insertion of a nuclear factor kappa B DNA nuclear-targeting sequence potentiates suicide gene therapy efficacy in lung cancer cell lines
- (2012) F Cramer et al. CANCER GENE THERAPY
- CHO cells in biotechnology for production of recombinant proteins: current state and further potential
- (2011) Jee Yon Kim et al. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
- Repeated integration of antibody genes into a pre-selected chromosomal locus of CHO cells using an accumulative site-specific gene integration system
- (2011) Yoshinori Kawabe et al. CYTOTECHNOLOGY
- Recombinase-Mediated Cassette Exchange (RMCE): Traditional Concepts and Current Challenges
- (2011) Soeren Turan et al. JOURNAL OF MOLECULAR BIOLOGY
- Monitoring of autophagy in Chinese hamster ovary cells using flow cytometry
- (2011) Jae Seong Lee et al. METHODS
- Immunoglobulin domain crossover as a generic approach for the production of bispecific IgG antibodies
- (2011) W. Schaefer et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Streamlined platform for short hairpin RNA interference and transgenesis in cultured mammalian cells
- (2011) P. Khandelia et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Progress and prospects: nuclear import of nonviral vectors
- (2010) A P Lam et al. GENE THERAPY
- Enhancing Antibody Fc Heterodimer Formation through Electrostatic Steering Effects
- (2010) Kannan Gunasekaran et al. JOURNAL OF BIOLOGICAL CHEMISTRY
- Multiplexing RMCE: Versatile Extensions of the Flp-Recombinase-Mediated Cassette-Exchange Technology
- (2010) Soeren Turan et al. JOURNAL OF MOLECULAR BIOLOGY
- Recombinase mediated cassette exchange into genomic targets using an adenovirus vector
- (2010) David A. Sorrell et al. NUCLEIC ACIDS RESEARCH
- Engineering Cell-Permeant FLP Recombinase for Tightly Controlled Inducible and Reversible Overexpression in Embryonic Stem Cells
- (2010) Christoph Patsch et al. STEM CELLS
- An accumulative site-specific gene integration system using cre recombinase-mediated cassette exchange
- (2009) Yujiro Kameyama et al. BIOTECHNOLOGY AND BIOENGINEERING
- An optimized extended DNA kappa B site that enhances plasmid DNA nuclear import and gene expression
- (2009) Cristine Gonçalves et al. JOURNAL OF GENE MEDICINE
- Novel Tag-and-Exchange (RMCE) Strategies Generate Master Cell Clones with Predictable and Stable Transgene Expression Properties
- (2009) Junhua Qiao et al. JOURNAL OF MOLECULAR BIOLOGY
- Improved production of recombinant human antithrombin III in Chinese hamster ovary cells by ATF4 overexpression
- (2007) Tomoshi Ohya et al. BIOTECHNOLOGY AND BIOENGINEERING
- Nutrient deprivation induces autophagy as well as apoptosis in Chinese hamster ovary cell culture
- (2007) Sun Ok Hwang et al. BIOTECHNOLOGY AND BIOENGINEERING
Publish scientific posters with Peeref
Peeref publishes scientific posters from all research disciplines. Our Diamond Open Access policy means free access to content and no publication fees for authors.
Learn MoreFind the ideal target journal for your manuscript
Explore over 38,000 international journals covering a vast array of academic fields.
Search