A platform for functional assessment of large variant libraries in mammalian cells

High-throughput discovery of post-transcriptional cis-regulatory elements

(2016) Erin M. Wissink et al.   BMC GENOMICS

Sort-seq under the hood: implications of design choices on large-scale characterization of sequence-function relations

(2016) Neil Peterman et al.   BMC GENOMICS

Prospective functional classification of all possible missense variants in PPARG

(2016) Amit R Majithia et al.   NATURE GENETICS

The power of multiplexed functional analysis of genetic variants

(2016) Molly Gasperini et al.   Nature Protocols

Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair

(2016) Xiangjun He et al.   NUCLEIC ACIDS RESEARCH

Phenotypic Characterization of a Comprehensive Set of MAPK1 /ERK2 Missense Mutants

(2016) Lisa Brenan et al.   Cell Reports

A modular open platform for systematic functional studies under physiological conditions

(2015) Christopher B. Mulholland et al.   NUCLEIC ACIDS RESEARCH

Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway

(2015) S.-E. Park et al.   SCIENCE

High-Resolution Sequence-Function Mapping of Full-Length Proteins

(2015) Caitlin A. Kowalsky et al.   PLoS One

The ins and outs of serine integrase site-specific recombination

(2014) Karen Rutherford et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Quantitative analysis of mammalian translation initiation sites by FACS-seq

(2014) W. L. Noderer et al.   Molecular Systems Biology

Saturation editing of genomic regions by multiplex homology-directed repair

(2014) Gregory M. Findlay et al.   NATURE

Deep mutational scanning: a new style of protein science

(2014) Douglas M Fowler et al.   NATURE METHODS

Measuring the activity of protein variants on a large scale using deep mutational scanning

(2014) Douglas M Fowler et al.   Nature Protocols

A platform for rapid prototyping of synthetic gene networks in mammalian cells

(2014) Xavier Duportet et al.   NUCLEIC ACIDS RESEARCH

The eukaryotic N-end rule pathway: conserved mechanisms and diverse functions

(2014) Daniel J. Gibbs et al.   TRENDS IN CELL BIOLOGY

Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations

(2014) Yao-Cheng Lin et al.   Nature Communications

Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome

(2013) Zhengyao Xu et al.   BMC BIOTECHNOLOGY

Chromatin Insulators: Linking Genome Organization to Cellular Function

(2013) Jennifer E. Phillips-Cremins et al.   MOLECULAR CELL

DICE, an efficient system for iterative genomic editing in human pluripotent stem cells

(2013) Fangfang Zhu et al.   NUCLEIC ACIDS RESEARCH

Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

(2013) Ophir Shalem et al.   SCIENCE

Genetic Screens in Human Cells Using the CRISPR-Cas9 System

(2013) Tim Wang et al.   SCIENCE

Counting absolute numbers of molecules using unique molecular identifiers

(2011) Teemu Kivioja et al.   NATURE METHODS

A Method for Producing Transgenic Cells Using a Multi-Integrase System on a Human Artificial Chromosome Vector

(2011) Shigeyuki Yamaguchi et al.   PLoS One

The N-end rule pathway and regulation by proteolysis

(2011) Alexander Varshavsky   PROTEIN SCIENCE

Structural basis of substrate recognition and specificity in the N-end rule pathway

(2010) Edna Matta-Camacho et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Accurately Measuring Recombination between Closely Related HIV-1 Genomes

(2010) Timothy E. Schlub et al.   PLoS Computational Biology

Quantification of plasmid DNA copies in the nucleus after lipoplex and polyplex transfection

(2009) Richard N. Cohen et al.   JOURNAL OF CONTROLLED RELEASE

Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases

(2009) Dirk Hockemeyer et al.   NATURE BIOTECHNOLOGY

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

The Molecular Basis of N-End Rule Recognition

(2008) Kevin H. Wang et al.   MOLECULAR CELL