Structural basis for the docking of mTORC1 on the lysosomal surface

Positive-unlabeled convolutional neural networks for particle picking in cryo-electron micrographs

(2019) Tristan Bepler et al.   NATURE METHODS

Architecture of the human GATOR1 and GATOR1–Rag GTPases complexes

(2018) Kuang Shen et al.   NATURE

Real-space refinement in PHENIX for cryo-EM and crystallography

(2018) Pavel V. Afonine et al.   Acta Crystallographica Section D-Structural Biology

Ragulator and SLC38A9 activate the Rag GTPases through noncanonical GEF mechanisms

(2018) Kuang Shen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

New tools for the analysis and validation of cryo-EM maps and atomic models

(2018) Pavel V. Afonine et al.   Acta Crystallographica Section D-Structural Biology

mTOR as a central hub of nutrient signalling and cell growth

(2018) Joungmok Kim et al.   NATURE CELL BIOLOGY

New tools for automated high-resolution cryo-EM structure determination in RELION-3

(2018) Jasenko Zivanov et al.   eLife

A Bayesian approach to beam-induced motion correction in cryo-EM single-particle analysis

(2018) Jasenko Zivanov et al.   IUCrJ

Intersubunit Crosstalk in the Rag GTPase Heterodimer Enables mTORC1 to Respond Rapidly to Amino Acid Availability

(2017) Kuang Shen et al.   MOLECULAR CELL

Hybrid Structure of the RagA/C-Ragulator mTORC1 Activation Complex

(2017) Ming-Yuan Su et al.   MOLECULAR CELL

Mechanisms of mTORC1 activation by RHEB and inhibition by PRAS40

(2017) Haijuan Yang et al.   NATURE

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

Addressing preferred specimen orientation in single-particle cryo-EM through tilting

(2017) Yong Zi Tan et al.   NATURE METHODS

Twenty-five years of mTOR: Uncovering the link from nutrients to growth

(2017) David M. Sabatini   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

UCSF ChimeraX: Meeting modern challenges in visualization and analysis

(2017) Thomas D. Goddard et al.   PROTEIN SCIENCE

Crystal structure of the human lysosomal mTORC1 scaffold complex and its impact on signaling

(2017) Mariana E. G. de Araujo et al.   SCIENCE

SAMTOR is anS-adenosylmethionine sensor for the mTORC1 pathway

(2017) Xin Gu et al.   SCIENCE

Structural basis for Ragulator functioning as a scaffold in membrane-anchoring of Rag GTPases and mTORC1

(2017) Tianlong Zhang et al.   Nature Communications

Structural basis for the assembly of the Ragulator-Rag GTPase complex

(2017) Ryo Yonehara et al.   Nature Communications

Structural insight into the Ragulator complex which anchors mTORC1 to the lysosomal membrane

(2017) Zongkai Mu et al.   Cell Discovery

Gctf: Real-time CTF determination and correction

(2016) Kai Zhang   JOURNAL OF STRUCTURAL BIOLOGY

CHARMM36m: an improved force field for folded and intrinsically disordered proteins

(2016) Jing Huang et al.   NATURE METHODS

Highly efficient baculovirus-mediated multigene delivery in primary cells

(2016) Maysam Mansouri et al.   Nature Communications

Recurrent mTORC1-activating RRAGC mutations in follicular lymphoma

(2015) Jessica Okosun et al.   NATURE GENETICS

EMRinger: side chain–directed model and map validation for 3D cryo-electron microscopy

(2015) Benjamin A Barad et al.   NATURE METHODS

Identification and characterization of essential genes in the human genome

(2015) T. Wang et al.   SCIENCE

The Caenorhabditis elegans protein SAS-5 forms large oligomeric assemblies critical for centriole formation

(2015) Kacper B Rogala et al.   eLife

Deciphering key features in protein structures with the new ENDscript server

(2014) Xavier Robert et al.   NUCLEIC ACIDS RESEARCH

Beam-induced motion correction for sub-megadalton cryo-EM particles

(2014) Sjors HW Scheres   eLife

Recruitment of folliculin to lysosomes supports the amino acid–dependent activation of Rag GTPases

(2013) Constance S. Petit et al.   JOURNAL OF CELL BIOLOGY

The Folliculin Tumor Suppressor Is a GAP for the RagC/D GTPases That Signal Amino Acid Levels to mTORC1

(2013) Zhi-Yang Tsun et al.   MOLECULAR CELL

One-pot DNA construction for synthetic biology: the Modular Overlap-Directed Assembly with Linkers (MODAL) strategy

(2013) Arturo Casini et al.   NUCLEIC ACIDS RESEARCH

OptimizedE. coliexpression strain LOBSTR eliminates common contaminants from His-tag purification

(2013) Kasper R. Andersen et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

A Tumor Suppressor Complex with GAP Activity for the Rag GTPases That Signal Amino Acid Sufficiency to mTORC1

(2013) L. Bar-Peled et al.   SCIENCE

Amino Acid Deprivation Inhibits TORC1 Through a GTPase-Activating Protein Complex for the Rag Family GTPase Gtr1

(2013) N. Panchaud et al.   Science Signaling

Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels to mTORC1

(2012) Liron Bar-Peled et al.   CELL

Crystal Structure of the Gtr1pGTP-Gtr2pGDPProtein Complex Reveals Large Structural Rearrangements Triggered by GTP-to-GDP Conversion

(2012) Jae-Hee Jeong et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Regulation of mTORC1 by the Rag GTPases is necessary for neonatal autophagy and survival

(2012) Alejo Efeyan et al.   NATURE

Structure-Function Relationships of the G Domain, a Canonical Switch Motif

(2011) Alfred Wittinghofer et al.   Annual Review of Biochemistry

Crystal structure of the Gtr1p-Gtr2p complex reveals new insights into the amino acid-induced TORC1 activation

(2011) R. Gong et al.   GENES & DEVELOPMENT

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Ragulator-Rag Complex Targets mTORC1 to the Lysosomal Surface and Is Necessary for Its Activation by Amino Acids

(2010) Yasemin Sancak et al.   CELL

MolProbity: all-atom structure validation for macromolecular crystallography

(2009) Vincent B. Chen et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

The novel lipid raft adaptor p18 controls endosome dynamics by anchoring the MEK–ERK pathway to late endosomes

(2009) Shigeyki Nada et al.   EMBO JOURNAL

Characterization of a novel splicing variant in the RAPTOR gene

(2009) Chang Sun et al.   MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Evaluating post-transcriptional regulatory elements for enhancing transient gene expression levels in CHO K1 and HEK293 cells

(2009) Mariati et al.   PROTEIN EXPRESSION AND PURIFICATION

The TSC1–TSC2 complex: a molecular switchboard controlling cell growth

(2008) Jingxiang Huang et al.   BIOCHEMICAL JOURNAL

Regulation of TORC1 by Rag GTPases in nutrient response

(2008) Eunjung Kim et al.   NATURE CELL BIOLOGY

The Rag GTPases Bind Raptor and Mediate Amino Acid Signaling to mTORC1

(2008) Y. Sancak et al.   SCIENCE