Protein Phase Separation Arising from Intrinsic Disorder: First-Principles to Bespoke Applications

Quantifying Viscosity and Surface Tension of Multi-Component Protein-Nucleic Acid Condensates

(2021) Ibraheem Alshareedah et al.   BIOPHYSICAL JOURNAL

Physics-based computational and theoretical approaches to intrinsically disordered proteins

(2021) Joan-Emma Shea et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Quantitative Study of the Interaction of Multivalent Ligand-Modified Nanoparticles with Breast Cancer Cells with Tunable Receptor Density

(2020) Jing Wang et al.   ACS Nano

Dynamic conformational flexibility and molecular interactions of intrinsically disordered proteins

(2020) Anil Bhattarai et al.   JOURNAL OF BIOSCIENCES

Phase separation provides a mechanism to reduce noise in cells

(2020) A. Klosin et al.   SCIENCE

De novo engineering of intracellular condensates using artificial disordered proteins

(2020) Michael Dzuricky et al.   Nature Chemistry

RNA contributions to the form and function of biomolecular condensates

(2020) Christine Roden et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Biomolecular Condensates in the Nucleus

(2020) Benjamin R. Sabari et al.   TRENDS IN BIOCHEMICAL SCIENCES

Relevance of Electrostatic Charges in Compactness, Aggregation, and Phase Separation of Intrinsically Disordered Proteins

(2020) Greta Bianchi et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Unraveling Molecular Interactions in Liquid–Liquid Phase Separation of Disordered Proteins by Atomistic Simulations

(2020) Matteo Paloni et al.   JOURNAL OF PHYSICAL CHEMISTRY B

A prion-like domain in ELF3 functions as a thermosensor in Arabidopsis

(2020) Jae-Hoon Jung et al.   NATURE

Phase separation at the nanoscale quantified by dcFCCS

(2020) Sijia Peng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Phosphoregulation of Phase Separation by the SARS-CoV-2 N Protein Suggests a Biophysical Basis for its Dual Functions

(2020) Christopher R. Carlson et al.   MOLECULAR CELL

Genomic RNA Elements Drive Phase Separation of the SARS-CoV-2 Nucleocapsid

(2020) Christiane Iserman et al.   MOLECULAR CELL

Membraneless organelles: P granules in C. elegans

(2019) Elisabeth A. Marnik et al.   TRAFFIC

Designer membraneless organelles enable codon reassignment of selected mRNAs in eukaryotes

(2019) Christopher D. Reinkemeier et al.   SCIENCE

The role of liquid-liquid phase separation in aggregation of the TDP-43 low complexity domain

(2019) W. Michael Babinchak et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates

(2019) Simon Alberti et al.   CELL

Engineered Ribonucleoprotein Granules Inhibit Translation in Protocells

(2019) Joseph R. Simon et al.   MOLECULAR CELL

Molecular interactions underlying liquid−liquid phase separation of the FUS low-complexity domain

(2019) Anastasia C. Murthy et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

RNA is a critical element for the sizing and the composition of phase-separated RNA–protein condensates

(2019) Marina Garcia-Jove Navarro et al.   Nature Communications

Interplay between Short-Range Attraction and Long-Range Repulsion Controls Reentrant Liquid Condensation of Ribonucleoprotein–RNA Complexes

(2019) Ibraheem Alshareedah et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Avidity and Cell Uptake of Integrin-Targeting Polypeptide Micelles is Strongly Shape-Dependent

(2019) Michael Dzuricky et al.   NANO LETTERS

First-generation predictors of biological protein phase separation

(2019) Robert M Vernon et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Phase separation-deficient TDP43 remains functional in splicing

(2019) Hermann Broder Schmidt et al.   Nature Communications

Intrinsically disordered proteins access a range of hysteretic phase separation behaviors

(2019) Felipe Garcia Quiroz et al.   Science Advances

Purification of proteins using peptide-ELP based affinity precipitation

(2019) Akshat Mullerpatan et al.   JOURNAL OF BIOTECHNOLOGY

Probing and engineering liquid-phase organelles

(2019) Dan Bracha et al.   NATURE BIOTECHNOLOGY

Relationship of Sequence and Phase Separation in Protein Low-Complexity Regions

(2018) Erik W. Martin et al.   BIOCHEMISTRY

P-Bodies: Composition, Properties, and Functions

(2018) Yang Luo et al.   BIOCHEMISTRY

Do Cellular Condensates Accelerate Biochemical Reactions? Lessons from Microdroplet Chemistry

(2018) Wylie Stroberg et al.   BIOPHYSICAL JOURNAL

The physiological and pathological biophysics of phase separation and gelation of RNA binding proteins in amyotrophic lateral sclerosis and fronto-temporal lobar degeneration

(2018) Peter St George-Hyslop et al.   BRAIN RESEARCH

A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins

(2018) Jie Wang et al.   CELL

Fusion of fibroblast growth factor 21 to a thermally responsive biopolymer forms an injectable depot with sustained anti-diabetic action

(2018) Caslin A. Gilroy et al.   JOURNAL OF CONTROLLED RELEASE

Advances in Understanding Stimulus-Responsive Phase Behavior of Intrinsically Disordered Protein Polymers

(2018) Kiersten M. Ruff et al.   JOURNAL OF MOLECULAR BIOLOGY

A Lattice Model of Charge-Pattern-Dependent Polyampholyte Phase Separation

(2018) Suman Das et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Mechanistic View of hnRNPA2 Low-Complexity Domain Structure, Interactions, and Phase Separation Altered by Mutation and Arginine Methylation

(2018) Veronica H. Ryan et al.   MOLECULAR CELL

Genetically encoded lipid–polypeptide hybrid biomaterials that exhibit temperature-triggered hierarchical self-assembly

(2018) Davoud Mozhdehi et al.   Nature Chemistry

The role of hydrophobic hydration in the LCST behaviour of POEGMA300 by all-atom molecular dynamics simulations

(2018) Eray Dalgakiran et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

Developing a molecular dynamics force field for both folded and disordered protein states

(2018) Paul Robustelli et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Physical principles of intracellular organization via active and passive phase transitions

(2018) Joel Berry et al.   REPORTS ON PROGRESS IN PHYSICS

RNA buffers the phase separation behavior of prion-like RNA binding proteins

(2018) Shovamayee Maharana et al.   SCIENCE

Phase separation of a yeast prion protein promotes cellular fitness

(2018) Titus M. Franzmann et al.   SCIENCE

Protein Phase Separation: A New Phase in Cell Biology

(2018) Steven Boeynaems et al.   TRENDS IN CELL BIOLOGY

Exploring Poly(ethylene glycol)-Polyzwitterion Diblock Copolymers as Biocompatible Smart Macrosurfactants Featuring UCST-Phase Behavior in Normal Saline Solution

(2018) Noverra Nizardo et al.   Polymers

Protein Phase Separation Provides Long-Term Memory of Transient Spatial Stimuli

(2018) Elliot Dine et al.   Cell Systems

Looking at the Disordered Proteins through the Computational Microscope

(2018) Payel Das et al.   ACS Central Science

A new temperature-dependent strategy to modulate the epidermal growth factor receptor

(2018) Zhe Li et al.   BIOMATERIALS

Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome

(2018) Yongdae Shin et al.   CELL

Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds

(2018) Dan Bracha et al.   CELL

Genetically Encoding Albumin Binding into Chemotherapeutic-loaded Polypeptide Nanoparticles Enhances Their Antitumor Efficacy

(2018) Parisa Yousefpour et al.   NANO LETTERS

Injectable tissue integrating networks from recombinant polypeptides with tunable order

(2018) Stefan Roberts et al.   NATURE MATERIALS

Salt-Dependent Rheology and Surface Tension of Protein Condensates Using Optical Traps

(2018) Louise M. Jawerth et al.   PHYSICAL REVIEW LETTERS

Long circulating genetically encoded intrinsically disordered zwitterionic polypeptides for drug delivery

(2018) Samagya Banskota et al.   BIOMATERIALS

Phase behaviour of disordered proteins underlying low density and high permeability of liquid organelles

(2017) Ming-Tzo Wei et al.   Nature Chemistry

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

TIA1 Mutations in Amyotrophic Lateral Sclerosis and Frontotemporal Dementia Promote Phase Separation and Alter Stress Granule Dynamics

(2017) Ian R. Mackenzie et al.   NEURON

Analysis of repetitive amino acid motifs reveals the essential features of spider dragline silk proteins

(2017) Ali D. Malay et al.   PLoS One

Liquid phase condensation in cell physiology and disease

(2017) Yongdae Shin et al.   SCIENCE

Local Nucleation of Microtubule Bundles through Tubulin Concentration into a Condensed Tau Phase

(2017) Amayra Hernández-Vega et al.   Cell Reports

Exploration of Protein Unfolding by Modelling Calorimetry Data from Reheating

(2017) Stanislav Mazurenko et al.   Scientific Reports

Programming Molecular Association and Viscoelastic Behavior in Protein Networks

(2016) Lawrence J. Dooling et al.   ADVANCED MATERIALS

Spatiotemporally photoradiation-controlled intratumoral depot for combination of brachytherapy and photodynamic therapy for solid tumor

(2016) Ratul Mukerji et al.   BIOMATERIALS

Wide-line NMR and DSC studies on intrinsically disordered p53 transactivation domain and its helically pre-structured segment

(2016) Peter Tompa et al.   BMB Reports

Compositional Control of Phase-Separated Cellular Bodies

(2016) Salman F. Banani et al.   CELL

Phase separation in biology; functional organization of a higher order

(2016) Diana M. Mitrea et al.   Cell Communication and Signaling

Using the folding landscapes of proteins to understand protein function

(2016) VV Hemanth Giri Rao et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

A non-chromatographic protein purification strategy using Src 3 homology domains as generalized capture domains

(2016) Heejae Kim et al.   JOURNAL OF BIOTECHNOLOGY

Sequence Determinants of Intracellular Phase Separation by Complex Coacervation of a Disordered Protein

(2016) Chi W. Pak et al.   MOLECULAR CELL

Sequence-Specific Polyampholyte Phase Separation in Membraneless Organelles

(2016) Yi-Hsuan Lin et al.   PHYSICAL REVIEW LETTERS

ALS Mutations Disrupt Phase Separation Mediated by α-Helical Structure in the TDP-43 Low-Complexity C-Terminal Domain

(2016) Alexander E. Conicella et al.   STRUCTURE

A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation

(2015) Avinash Patel et al.   CELL

Elastin-like polypeptides as models of intrinsically disordered proteins

(2015) Stefan Roberts et al.   FEBS LETTERS

Prion-like domains in RNA binding proteins are essential for building subnuclear paraspeckles

(2015) Sven Hennig et al.   JOURNAL OF CELL BIOLOGY

Residue-by-Residue View of In Vitro FUS Granules that Bind the C-Terminal Domain of RNA Polymerase II

(2015) Kathleen A. Burke et al.   MOLECULAR CELL

Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles

(2015) Timothy J. Nott et al.   MOLECULAR CELL

Sequence heuristics to encode phase behaviour in intrinsically disordered protein polymers

(2015) Felipe García Quiroz et al.   NATURE MATERIALS

Polymer physics of intracellular phase transitions

(2015) Clifford P. Brangwynne et al.   Nature Physics

New aspects in the phase behaviour of poly-N-isopropyl acrylamide: systematic temperature dependent shrinking of PNiPAM assemblies well beyond the LCST

(2015) Irmgard Bischofberger et al.   Scientific Reports

Studies on thermoresponsive polymers: Phase behaviour, drug delivery and biomedical applications

(2015) Arijit Gandhi et al.   Asian Journal of Pharmaceutical Sciences

An16-resilin: An advanced multi-stimuli-responsive resilin-mimetic protein polymer

(2014) Rajkamal Balu et al.   Acta Biomaterialia

Conditionally and Transiently Disordered Proteins: Awakening Cryptic Disorder To Regulate Protein Function

(2014) Ursula Jakob et al.   CHEMICAL REVIEWS

Introduction to Intrinsically Disordered Proteins (IDPs)

(2014) Vladimir N. Uversky   CHEMICAL REVIEWS

Exploring Free-Energy Landscapes of Intrinsically Disordered Proteins at Atomic Resolution Using NMR Spectroscopy

(2014) Malene Ringkjøbing Jensen et al.   CHEMICAL REVIEWS

Classification of Intrinsically Disordered Regions and Proteins

(2014) Robin van der Lee et al.   CHEMICAL REVIEWS

Noncanonical Self-Assembly of Highly Asymmetric Genetically Encoded Polypeptide Amphiphiles into Cylindrical Micelles

(2014) Jonathan R. McDaniel et al.   NANO LETTERS

Three-in-One Chromatography-Free Purification, Tag Removal, and Site-Specific Modification of Recombinant Fusion Proteins Using Sortase A and Elastin-like Polypeptides

(2013) Joseph J. Bellucci et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Impact and progress in small and wide angle X-ray scattering (SAXS and WAXS)

(2013) Melissa A Graewert et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

A depot-forming glucagon-like peptide-1 fusion protein reduces blood glucose for five days with a single injection

(2013) M. Amiram et al.   JOURNAL OF CONTROLLED RELEASE

Structural Characterization of Intrinsically Disordered Proteins by NMR Spectroscopy

(2013) Simone Kosol et al.   MOLECULES

Polymers with Upper Critical Solution Temperature in Aqueous Solution: Unexpected Properties from Known Building Blocks

(2013) Jan Seuring et al.   ACS Macro Letters

Self-Assembly of Thermally Responsive Nanoparticles of a Genetically Encoded Peptide Polymer by Drug Conjugation

(2012) Jonathan R. McDaniel et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A dual ELP-tagged split intein system for non-chromatographic recombinant protein purification

(2012) Changhua Shi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Differential scanning calorimetry as a tool for protein folding and stability

(2012) Christopher M. Johnson   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Purification of recombinant protein by cold-coacervation of fusion constructs incorporating resilin-inspired polypeptides

(2012) Russell E. Lyons et al.   BIOTECHNOLOGY AND BIOENGINEERING

The tip of the iceberg: RNA-binding proteins with prion-like domains in neurodegenerative disease

(2012) Oliver D. King et al.   BRAIN RESEARCH

Brachytherapy Using Injectable Seeds That Are Self-Assembled from Genetically Encoded Polypeptides In Situ

(2012) W. Liu et al.   CANCER RESEARCH

ELP-z and ELP-zz capturing scaffolds for the purification of immunoglobulins by affinity precipitation

(2012) Bhawna Madan et al.   JOURNAL OF BIOTECHNOLOGY

Phase transitions in the assembly of multivalent signalling proteins

(2012) Pilong Li et al.   NATURE

Systematic Validation of Protein Force Fields against Experimental Data

(2012) Kresten Lindorff-Larsen et al.   PLoS One

Tunable Self-Assembly of Genetically Engineered Silk–Elastin-like Protein Polymers

(2011) Xiao-Xia Xia et al.   BIOMACROMOLECULES

Protein Purification by Polyelectrolyte Coacervation: Influence of Protein Charge Anisotropy on Selectivity

(2011) Yisheng Xu et al.   BIOMACROMOLECULES

Upper Critical Solution Temperature of Poly(N-acryloyl glycinamide) in Water: A Concealed Property

(2011) Jan Seuring et al.   MACROMOLECULES

A highly parallel method for synthesizing DNA repeats enables the discovery of ‘smart’ protein polymers

(2011) Miriam Amiram et al.   NATURE MATERIALS

Thermoresponsive behaviour of poly[(oligo(ethyleneglycol methacrylate)]s and their protein conjugates: importance of concentration and solvent system

(2011) Konstantinos Bebis et al.   Polymer Chemistry

UCST-type behavior of poly[oligo(ethylene glycol) methyl ether methacrylate] (POEGMA) in aliphatic alcohols: solvent, co-solvent, molecular weight, and end group dependences

(2011) Peter J. Roth et al.   Soft Matter

Elastin-like polypeptides: Biomedical applications of tunable biopolymers

(2010) Sarah R. MacEwan et al.   BIOPOLYMERS

Injectable intratumoral depot of thermally responsive polypeptide–radionuclide conjugates delays tumor progression in a mouse model

(2010) Wenge Liu et al.   JOURNAL OF CONTROLLED RELEASE

Self-oscillating gel composed of thermosensitive polymer exhibiting higher LCST

(2010) Mio Hidaka et al.   JOURNAL OF CONTROLLED RELEASE

Determination of the Free Energy Landscape of α-Synuclein Using Spin Label Nuclear Magnetic Resonance Measurements

(2009) Jane R. Allison et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation

(2009) C. P. Brangwynne et al.   SCIENCE

Structural Polymorphism of 441-Residue Tau at Single Residue Resolution

(2009) Marco D Mukrasch et al.   PLOS BIOLOGY

Secondary Structure Formation and LCST Behavior of Short Elastin-Like Peptides

(2008) Harald Nuhn et al.   BIOMACROMOLECULES

A simple patchy colloid model for the phase behavior of lysozyme dispersions

(2008) Christoph Gögelein et al.   JOURNAL OF CHEMICAL PHYSICS

Allosteric Actuation of Inverse Phase Transition of a Stimulus-Responsive Fusion Polypeptide by Ligand Binding

(2008) Bumjoon Kim et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Structure of tumor suppressor p53 and its intrinsically disordered N-terminal transactivation domain

(2008) M. Wells et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In Situ Cross-Linking of Elastin-like Polypeptide Block Copolymers for Tissue Repair

(2007) Dong Woo Lim et al.   BIOMACROMOLECULES

Temperature Triggered Self-Assembly of Polypeptides into Multivalent Spherical Micelles

(2007) Matthew R. Dreher et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY