CRISPR: History and perspectives to the future

CRISPR/Cas9: Principle, Applications, and Delivery through Extracellular Vesicles

(2021) Katarzyna Horodecka et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Regulation of the RNA and DNA nuclease activities required for Pyrococcus furiosus Type III-B CRISPR–Cas immunity

(2020) Kawanda Foster et al.   NUCLEIC ACIDS RESEARCH

Gene-edited human stem cell–derived β cells from a patient with monogenic diabetes reverse preexisting diabetes in mice

(2020) Kristina G. Maxwell et al.   Science Translational Medicine

Targeting Epigenetic Modifications in Uveal Melanoma

(2020) Pooneh Chokhachi Baradaran et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Single C-to-T substitution using engineered APOBEC3G-nCas9 base editors with minimum genome- and transcriptome-wide off-target effects

(2020) Sangsin Lee et al.   Science Advances

CRISPR Gene Therapy: Applications, Limitations, and Implications for the Future

(2020) Fathema Uddin et al.   Frontiers in Oncology

CRISPR/Cas gene therapy

(2020) Baohong Zhang   JOURNAL OF CELLULAR PHYSIOLOGY

Application of different types of CRISPR/Cas-based systems in bacteria

(2020) Zhenquan Liu et al.   Microbial Cell Factories

CRISPR technology: The engine that drives cancer therapy

(2020) Mingtao Zhang et al.   BIOMEDICINE & PHARMACOTHERAPY

Engineering precision nanoparticles for drug delivery

(2020) Michael J. Mitchell et al.   NATURE REVIEWS DRUG DISCOVERY

Precise and broad scope genome editing based on high-specificity Cas9 nickases

(2020) Qian Wang et al.   NUCLEIC ACIDS RESEARCH

Identification of preexisting adaptive immunity to Cas9 proteins in humans

(2019) Carsten T. Charlesworth et al.   NATURE MEDICINE

CRISPR-Cas9 for cancer therapy: Opportunities and challenges

(2019) Minjiang Chen et al.   CANCER LETTERS

Targeting Plant ssDNA Viruses with Engineered Miniature CRISPR-Cas14a

(2019) Muhammad Zuhaib Khan et al.   TRENDS IN BIOTECHNOLOGY

Delivery of CRISPR/Cas9 for therapeutic genome editing

(2019) Xiaojie Xu et al.   JOURNAL OF GENE MEDICINE

CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites

(2019) Rajeswari Jayavaradhan et al.   Nature Communications

Dead Cas Systems: Types, Principles, and Applications

(2019) Sergey Brezgin et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

CRISPR/Cas System: Recent Advances and Future Prospects for Genome Editing

(2019) Hakim Manghwar et al.   TRENDS IN PLANT SCIENCE

Thermo-triggered Release of CRISPR-Cas9 System by Lipid-Encapsulated Gold Nanoparticles for Tumor Therapy

(2018) Peng Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Delivering CRISPR: a review of the challenges and approaches

(2018) Christopher A. Lino et al.   DRUG DELIVERY

Hallmarks of cancer: The CRISPR generation

(2018) Colette Moses et al.   EUROPEAN JOURNAL OF CANCER

Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements

(2018) Michael Kosicki et al.   NATURE BIOTECHNOLOGY

CRISPR-enhanced engineering of therapy-sensitive cancer cells for self-targeting of primary and metastatic tumors

(2018) Clemens Reinshagen et al.   Science Translational Medicine

Nucleosomes inhibit target cleavage by CRISPR-Cas9 in vivo

(2018) Robert M. Yarrington et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Systematic discovery of natural CRISPR-Cas12a inhibitors

(2018) Kyle E. Watters et al.   SCIENCE

Directed evolution of CRISPR-Cas9 to increase its specificity

(2018) Jungjoon K. Lee et al.   Nature Communications

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes

(2018) Lucas B. Harrington et al.   SCIENCE

Cancer-derived exosomes as a delivery platform of CRISPR/Cas9 confer cancer cell tropism-dependent targeting

(2017) Seung Min Kim et al.   JOURNAL OF CONTROLLED RELEASE

Synthetically modified guide RNA and donor DNA are a versatile platform for CRISPR-Cas9 engineering

(2017) Kunwoo Lee et al.   eLife

CRISPR/Cas9 Immune System as a Tool for Genome Engineering

(2016) Magdalena Hryhorowicz et al.   ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS

Naturally Occurring Off-Switches for CRISPR-Cas9

(2016) April Pawluk et al.   CELL

Engineered Viruses as Genome Editing Devices

(2016) Xiaoyu Chen et al.   MOLECULAR THERAPY

Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo

(2016) Hao Yin et al.   NATURE BIOTECHNOLOGY

Repurposing the CRISPR-Cas9 system for targeted DNA methylation

(2016) Aleksandar Vojta et al.   NUCLEIC ACIDS RESEARCH

Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice

(2016) Roman Maresch et al.   Nature Communications

Waking up dormant tumor suppressor genes with zinc fingers, TALEs and the CRISPR/dCas9 system

(2016) Benjamin Garcia-Bloj et al.   Oncotarget

CRISPR-Mediated VHL Knockout Generates an Improved Model for Metastatic Renal Cell Carcinoma

(2016) Shiruyeh Schokrpur et al.   Scientific Reports

Nucleosomes impede Cas9 access to DNA in vivo and in vitro

(2016) Max A Horlbeck et al.   eLife

CRISPR-Cas9D10A nickase-based genotypic and phenotypic screening to enhance genome editing

(2016) Ting-Wei Will Chiang et al.   Scientific Reports

Advantages of using the CRISPR/Cas9 system of genome editing to investigate male reproductive mechanisms using mouse models

(2015) Masahito Ikawa et al.   ASIAN JOURNAL OF ANDROLOGY

Cancer-Associated Protein Kinase C Mutations Reveal Kinase’s Role as Tumor Suppressor

(2015) Corina E. Antal et al.   CELL

Pancreatic cancer modeling using retrograde viral vector delivery and in vivo CRISPR/Cas9-mediated somatic genome editing

(2015) Shin-Heng Chiou et al.   GENES & DEVELOPMENT

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

(2015) Sergey Shmakov et al.   MOLECULAR CELL

Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining

(2015) Takeshi Maruyama et al.   NATURE BIOTECHNOLOGY

Modeling colorectal cancer using CRISPR-Cas9–mediated engineering of human intestinal organoids

(2015) Mami Matano et al.   NATURE MEDICINE

Highly efficient Cas9-mediated transcriptional programming

(2015) Alejandro Chavez et al.   NATURE METHODS

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Cripto-1 as a novel therapeutic target for triple negative breast cancer

(2015) Nadia P. Castro et al.   Oncotarget

CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling

(2014) Randall J. Platt et al.   CELL

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

(2014) Hiroshi Nishimasu et al.   CELL

The clinical impact of deficiency in DNA non-homologous end-joining

(2014) Lisa Woodbine et al.   DNA REPAIR

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

(2014) S. Kim et al.   GENOME RESEARCH

CRISPR-mediated direct mutation of cancer genes in the mouse liver

(2014) Wen Xue et al.   NATURE

Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing

(2014) Dirk Heckl et al.   NATURE BIOTECHNOLOGY

Homology-directed repair of DNA nicks via pathways distinct from canonical double-strand break repair

(2014) L. Davis et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation

(2014) M. Jinek et al.   SCIENCE

Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System

(2013) Baohui Chen et al.   CELL

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Bacterial natural transformation by highly fragmented and damaged DNA

(2013) S. Overballe-Petersen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

RNA-guided genetic silencing systems in bacteria and archaea

(2012) Blake Wiedenheft et al.   NATURE

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

DNA repair by nonhomologous end joining and homologous recombination during cell cycle in human cells

(2011) Zhiyong Mao et al.   CELL CYCLE

Genetic engineering of human pluripotent cells using TALE nucleases

(2011) Dirk Hockemeyer et al.   NATURE BIOTECHNOLOGY

State-of-the-art gene-based therapies: the road ahead

(2011) Mark A. Kay   NATURE REVIEWS GENETICS

Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence

(2011) E. Semenova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genome editing with engineered zinc finger nucleases

(2010) Fyodor D. Urnov et al.   NATURE REVIEWS GENETICS