Modulating α-Synuclein Liquid–Liquid Phase Separation

Reentrant liquid condensate phase of proteins is stabilized by hydrophobic and non-ionic interactions

(2021) Georg Krainer et al.   Nature Communications

Synapsin Condensates Recruit alpha-Synuclein

(2021) Christian Hoffmann et al.   JOURNAL OF MOLECULAR BIOLOGY

Super-resolution imaging reveals α-synuclein seeded aggregation in SH-SY5Y cells

(2021) Jason C. Sang et al.   Communications Biology

Phase-separation in chromatin organization

(2020) Geeta J Narlikar   JOURNAL OF BIOSCIENCES

Liquid-like droplet formation by tumor suppressor p53 induced by multivalent electrostatic interactions between two disordered domains

(2020) Kiyoto Kamagata et al.   Scientific Reports

Composition-dependent thermodynamics of intracellular phase separation

(2020) Joshua A. Riback et al.   NATURE

Liquid–liquid phase separation of type II diabetes-associated IAPP initiates hydrogelation and aggregation

(2020) Lior Pytowski et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Role of Liquid–Liquid Phase Separation in the Compartmentalization of Cell Nucleus and Spatial Genome Organization

(2020) S. V. Razin et al.   BIOCHEMISTRY-MOSCOW

α-Synuclein aggregation nucleates through liquid–liquid phase separation

(2020) Soumik Ray et al.   Nature Chemistry

De novo engineering of intracellular condensates using artificial disordered proteins

(2020) Michael Dzuricky et al.   Nature Chemistry

Phase transition of RNA−protein complexes into ordered hollow condensates

(2020) Ibraheem Alshareedah et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Partitioning of cancer therapeutics in nuclear condensates

(2020) Isaac A. Klein et al.   SCIENCE

Impact of solid surface hydrophobicity and micrococcal nuclease production on Staphylococcus aureus Newman biofilms

(2020) Abigail M. Forson et al.   Scientific Reports

Phase Separation and Neurodegenerative Diseases: A Disturbance in the Force

(2020) Aurélie Zbinden et al.   DEVELOPMENTAL CELL

Phase Separation—A Physical Mechanism for Organizing Information and Biochemical Reactions

(2020) Rohit V. Pappu   DEVELOPMENTAL CELL

Predictable phase-separated proteins

(2020) Soumik Ray et al.   Nature Chemistry

Beyond aggregation: Pathological phase transitions in neurodegenerative disease

(2020) Cécile Mathieu et al.   SCIENCE

Aggresome formation and liquid–liquid phase separation independently induce cytoplasmic aggregation of TAR DNA-binding protein 43

(2020) Seiji Watanabe et al.   Cell Death & Disease

Purification of Recombinant α-synuclein: A Comparison of Commonly Used Protocols

(2020) Amberley D. Stephens et al.   BIOCHEMISTRY

Widespread occurrence of the droplet state of proteins in the human proteome

(2020) Maarten Hardenberg et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Observation of an α-synuclein liquid droplet state and its maturation into Lewy body-like assemblies

(2020) Maarten C Hardenberg et al.   Journal of Molecular Cell Biology

Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates

(2019) Simon Alberti et al.   CELL

α-Synuclein misfolding and aggregation: Implications in Parkinson’s disease pathogenesis

(2019) Surabhi Mehra et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

A first order phase transition mechanism underlies protein aggregation in mammalian cells

(2019) Arjun Narayanan et al.   eLife

Heterochromatin drives compartmentalization of inverted and conventional nuclei

(2019) Martin Falk et al.   NATURE

Phase separation and clustering of an ABC transporter in Mycobacterium tuberculosis

(2019) Florian Heinkel et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Liquid–Liquid Phase Separation in Disease

(2019) Simon Alberti et al.   Annual Review of Genetics

N-Terminal Acetylation Affects α-Synuclein Fibril Polymorphism

(2019) Matthew D. Watson et al.   BIOCHEMISTRY

Organization of Chromatin by Intrinsic and Regulated Phase Separation

(2019) Bryan A. Gibson et al.   CELL

The Role of Phase Separation in Heterochromatin Formation, Function, and Regulation

(2018) Adam G. Larson et al.   BIOCHEMISTRY

Functional Implications of Intracellular Phase Transitions

(2018) Alex S. Holehouse et al.   BIOCHEMISTRY

A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins

(2018) Jie Wang et al.   CELL

Tau protein liquid–liquid phase separation can initiate tau aggregation

(2018) Susanne Wegmann et al.   EMBO JOURNAL

A User’s Guide for Phase Separation Assays with Purified Proteins

(2018) Simon Alberti et al.   JOURNAL OF MOLECULAR BIOLOGY

Exogenous α-synuclein hinders synaptic communication in cultured cortical primary rat neurons

(2018) G. C. Hassink et al.   PLoS One

RNA buffers the phase separation behavior of prion-like RNA binding proteins

(2018) Shovamayee Maharana et al.   SCIENCE

Atomic structures of low-complexity protein segments reveal kinked β sheets that assemble networks

(2018) Michael P. Hughes et al.   SCIENCE

Protein Phase Separation: A New Phase in Cell Biology

(2018) Steven Boeynaems et al.   TRENDS IN CELL BIOLOGY

Transcription Factors Activate Genes through the Phase-Separation Capacity of Their Activation Domains

(2018) Ann Boija et al.   CELL

Phase separation drives heterochromatin domain formation

(2017) Amy R. Strom et al.   NATURE

α-synuclein toxicity in neurodegeneration: mechanism and therapeutic strategies

(2017) Yvette C Wong et al.   NATURE MEDICINE

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Liquid phase condensation in cell physiology and disease

(2017) Yongdae Shin et al.   SCIENCE

Liquid–liquid phase separation of the microtubule-binding repeats of the Alzheimer-related protein Tau

(2017) Susmitha Ambadipudi et al.   Nature Communications

Phosphorylated α-Synuclein-Copper Complex Formation in the Pathogenesis of Parkinson’s Disease

(2017) Juan Antonio Castillo-Gonzalez et al.   Parkinsons Disease

Effect of acidic pH on the stability of α-synuclein dimers

(2016) Zhengjian Lv et al.   BIOPOLYMERS

Amyloid-like Self-Assembly of a Cellular Compartment

(2016) Elvan Boke et al.   CELL

Compositional Control of Phase-Separated Cellular Bodies

(2016) Salman F. Banani et al.   CELL

Coexisting Liquid Phases Underlie Nucleolar Subcompartments

(2016) Marina Feric et al.   CELL

Phase separation in biology; functional organization of a higher order

(2016) Diana M. Mitrea et al.   Cell Communication and Signaling

Liquid–liquid phase separation in cellular signaling systems

(2016) P Andrew Chong et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Sequence Determinants of Intracellular Phase Separation by Complex Coacervation of a Disordered Protein

(2016) Chi W. Pak et al.   MOLECULAR CELL

Phase separation of signaling molecules promotes T cell receptor signal transduction

(2016) Xiaolei Su et al.   SCIENCE

ALS Mutations Disrupt Phase Separation Mediated by α-Helical Structure in the TDP-43 Low-Complexity C-Terminal Domain

(2016) Alexander E. Conicella et al.   STRUCTURE

Phase Separation: Linking Cellular Compartmentalization to Disease

(2016) Adriano Aguzzi et al.   TRENDS IN CELL BIOLOGY

The Transcellular Propagation and Intracellular Trafficking of α-Synuclein

(2016) George K. Tofaris et al.   Cold Spring Harbor Perspectives in Medicine

A pH-driven transition of the cytoplasm from a fluid- to a solid-like state promotes entry into dormancy

(2016) Matthias Christoph Munder et al.   eLife

Phase Separation by Low Complexity Domains Promotes Stress Granule Assembly and Drives Pathological Fibrillization

(2015) Amandine Molliex et al.   CELL

A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation

(2015) Avinash Patel et al.   CELL

RNA Controls PolyQ Protein Phase Transitions

(2015) Huaiying Zhang et al.   MOLECULAR CELL

Formation and Maturation of Phase-Separated Liquid Droplets by RNA-Binding Proteins

(2015) Yuan Lin et al.   MOLECULAR CELL

Residue-by-Residue View of In Vitro FUS Granules that Bind the C-Terminal Domain of RNA Polymerase II

(2015) Kathleen A. Burke et al.   MOLECULAR CELL

Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles

(2015) Timothy J. Nott et al.   MOLECULAR CELL

Polymer physics of intracellular phase transitions

(2015) Clifford P. Brangwynne et al.   Nature Physics

ALS/FTD Mutation-Induced Phase Transition of FUS Liquid Droplets and Reversible Hydrogels into Irreversible Hydrogels Impairs RNP Granule Function

(2015) Tetsuro Murakami et al.   NEURON

Liquid-Liquid Phase Separation in Biology

(2014) Anthony A. Hyman et al.   Annual Review of Cell and Developmental Biology

A reduced dimensionality NMR pulse sequence and an efficient protocol for unambiguous assignment in intrinsically disordered proteins

(2014) Jithender G. Reddy et al.   JOURNAL OF BIOMOLECULAR NMR

The Presence of an Air–Water Interface Affects Formation and Elongation of α-Synuclein Fibrils

(2014) Silvia Campioni et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Solution conditions determine the relative importance of nucleation and growth processes in  -synuclein aggregation

(2014) A. K. Buell et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Phase transitions of multivalent proteins can promote clustering of membrane receptors

(2014) Sudeep Banjade et al.   eLife

N-terminal Acetylation Stabilizes N-terminal Helicity in Lipid- and Micelle-bound α-Synuclein and Increases Its Affinity for Physiological Membranes

(2013) Igor Dikiy et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Curcumin Modulates α-Synuclein Aggregation and Toxicity

(2012) Pradeep K. Singh et al.   ACS Chemical Neuroscience

On-Surface Aggregation of α-Synuclein at Nanomolar Concentrations Results in Two Distinct Growth Mechanisms

(2012) Michael Rabe et al.   ACS Chemical Neuroscience

Impact of N-Terminal Acetylation of α-Synuclein on Its Random Coil and Lipid Binding Properties

(2012) Alexander S. Maltsev et al.   BIOCHEMISTRY

Phase transitions in the assembly of multivalent signalling proteins

(2012) Pilong Li et al.   NATURE

The many faces of α-synuclein: from structure and toxicity to therapeutic target

(2012) Hilal A. Lashuel et al.   NATURE REVIEWS NEUROSCIENCE

Raised calcium and oxidative stress cooperatively promote alpha-synuclein aggregate formation

(2012) J. Goodwin et al.   NEUROCHEMISTRY INTERNATIONAL

Crystallization and liquid-liquid phase separation of monoclonal antibodies and fc-fusion proteins: Screening results

(2011) Egor Trilisky et al.   BIOTECHNOLOGY PROGRESS

Assays for α-synuclein aggregation

(2010) Lise Giehm et al.   METHODS

Raised calcium promotes α-synuclein aggregate formation

(2010) S. Nath et al.   MOLECULAR AND CELLULAR NEUROSCIENCE

Characterization of Hydrophobic Residue Requirements for α-Synuclein Fibrillization

(2009) Elisa A. Waxman et al.   BIOCHEMISTRY

Widespread reorganization of metabolic enzymes into reversible assemblies upon nutrient starvation

(2009) R. Narayanaswamy et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation

(2009) C. P. Brangwynne et al.   SCIENCE

De Novo Formation of a Subnuclear Body

(2008) T. E. Kaiser et al.   SCIENCE

Protein Phase Behavior in Aqueous Solutions: Crystallization, Liquid-Liquid Phase Separation, Gels, and Aggregates

(2007) André C. Dumetz et al.   BIOPHYSICAL JOURNAL

Calcium(II) selectively induces α-synuclein annular oligomers via interaction with the C-terminal domain

(2004) Rachel Lowe et al.   PROTEIN SCIENCE