In Vitro Assembly of Virus-Like Particles and Their Applications

How a Virus Circumvents Energy Barriers to Form Symmetric Shells

(2020) Sanaz Panahandeh et al.   ACS Nano

Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation

(2020) Daniel Wrapp et al.   SCIENCE

Synthesis and Assembly of Hepatitis B Virus-Like Particles in a Pichia pastoris Cell-Free System

(2020) Alex J. Spice et al.   Frontiers in Bioengineering and Biotechnology

Influenza Hemagglutinin Nanoparticle Vaccine Elicits Broadly Neutralizing Antibodies against Structurally Distinct Domains of H3N2 HA

(2020) Alyse D. Portnoff et al.   Vaccines

Virus-Like Particles (VLPs) as a Platform for Hierarchical Compartmentalization

(2020) Hitesh Kumar Waghwani et al.   BIOMACROMOLECULES

Revealing in real-time a multistep assembly mechanism for SV40 virus-like particles

(2020) Mariska G. M. van Rosmalen et al.   Science Advances

Recent Progress on the Versatility of Virus-Like Particles

(2020) Ciying Qian et al.   Vaccines

Visualization of Single Molecules Building a Viral Capsid Protein Lattice through Stochastic Pathways

(2020) Alejandro Valbuena et al.   ACS Nano

COVID-19 vaccine development and a potential nanomaterial path forward

(2020) Matthew D. Shin et al.   Nature Nanotechnology

Phase 1–2 Trial of a SARS-CoV-2 Recombinant Spike Protein Nanoparticle Vaccine

(2020) Cheryl Keech et al.   NEW ENGLAND JOURNAL OF MEDICINE

Liver-specific Gene Delivery Using Engineered Virus-Like Particles of Hepatitis E Virus

(2019) Eun Byul Lee et al.   Scientific Reports

Efficacy, safety, and immunogenicity of an Escherichia coli-produced bivalent human papillomavirus vaccine: An interim analysis of a randomized clinical trial

(2019) You-Lin Qiao et al.   JNCI-Journal of the National Cancer Institute

Assembly Reactions of Hepatitis B Capsid Protein into Capsid Nanoparticles Follow a Narrow Path through a Complex Reaction Landscape

(2019) Roi Asor et al.   ACS Nano

Delivery of self-amplifying RNA vaccines in in vitro reconstituted virus-like particles

(2019) Adam Biddlecome et al.   PLoS One

Mass spectrometry-based studies of virus assembly

(2019) Alison E Ashcroft   Current Opinion in Virology

Synthetic Biology Goes Cell-Free

(2019) Aidan Tinafar et al.   BMC BIOLOGY

Polymorphic assembly of virus-capsid proteins around DNA and the cellular uptake of the resulting particles

(2019) M.V. de Ruiter et al.   JOURNAL OF CONTROLLED RELEASE

Real-Time Assembly of Viruslike Nucleocapsids Elucidated at the Single-Particle Level

(2019) Margherita Marchetti et al.   NANO LETTERS

Measurements of the self-assembly kinetics of individual viral capsids around their RNA genome

(2019) Rees F. Garmann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Production and characterization of virus-like particles of grapevine fanleaf virus presenting L2 epitope of human papillomavirus minor capsid protein

(2019) Razieh Yazdani et al.   BMC BIOTECHNOLOGY

Structural transitions during the scaffolding-driven assembly of a viral capsid

(2019) Athanasios Ignatiou et al.   Nature Communications

VLPs Derived from the CCMV Plant Virus Can Directly Transfect and Deliver Heterologous Genes for Translation into Mammalian Cells

(2019) María V. Villagrana-Escareño et al.   Biomed Research International

Adeno-associated virus capsid protein expression in Escherichia coli and chemically defined capsid assembly

(2019) Dinh To Le et al.   Scientific Reports

A Universal Plug-and-Display Vaccine Carrier Based on HBsAg VLP to Maximize Effective Antibody Response

(2019) Arianna Marini et al.   Frontiers in Immunology

Programmable In Vitro Coencapsidation of Guest Proteins for Intracellular Delivery by Virus-like Particles

(2018) Noor H. Dashti et al.   ACS Nano

Applications of high-speed atomic force microscopy to real-time visualization of dynamic biomolecular processes

(2018) Takayuki Uchihashi et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Multiple Pathways in Capsid Assembly

(2018) Corinne A. Lutomski et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Templated Formation of Luminescent Virus-like Particles by Tailor-Made Pt(II) Amphiphiles

(2018) Stephan Sinn et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Modularized peptides modified HBc virus-like particles for encapsulation and tumor-targeted delivery of doxorubicin

(2018) Wenjun Shan et al.   Nanomedicine-Nanotechnology Biology and Medicine

Characterization of capsid protein (p495) of hepatitis E virus expressed in Escherichia coli and assembling into particles in vitro

(2018) Minghua Zheng et al.   VACCINE

Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations

(2018) Jaison Jeevanandam et al.   Beilstein Journal of Nanotechnology

Hepatitis B virus infection

(2018) Man-Fung Yuen et al.   Nature Reviews Disease Primers

Nonequilibrium self-assembly dynamics of icosahedral viral capsids packaging genome or polyelectrolyte

(2018) Maelenn Chevreuil et al.   Nature Communications

Efficient support of virus-like particle assembly by the HIV-1 packaging signal

(2018) Mauricio Comas-Garcia et al.   eLife

N-terminal truncations on L1 proteins of human papillomaviruses promote their soluble expression in Escherichia coli and self-assembly in vitro

(2018) Minxi Wei et al.   Emerging Microbes & Infections

Modular interior loading and exterior decoration of a virus-like particle

(2017) Jhanvi Sharma et al.   Nanoscale

Characterization of an Escherichia coli -derived human papillomavirus type 16 and 18 bivalent vaccine

(2017) Ying Gu et al.   VACCINE

Novel hemagglutinin nanoparticle influenza vaccine with Matrix-M™ adjuvant induces hemagglutination inhibition, neutralizing, and protective responses in ferrets against homologous and drifted A(H3N2) subtypes

(2017) Gale Smith et al.   VACCINE

Immunogenicity and safety of a respiratory syncytial virus fusion protein (RSV F) nanoparticle vaccine in older adults

(2017) Louis Fries et al.   Immunity & Ageing

Efficient Production of Papillomavirus Gene Delivery Vectors in Defined In Vitro Reactions

(2017) Carla Cerqueira et al.   Molecular Therapy-Methods & Clinical Development

A viral scaffolding protein triggers portal ring oligomerization and incorporation during procapsid assembly

(2017) Tina Motwani et al.   Science Advances

Escherichia coli-derived virus-like particles in vaccine development

(2017) Xiaofen Huang et al.   npj Vaccines

Yield Optimisation of Hepatitis B Virus Core Particles in E. coli Expression System for Drug Delivery Applications

(2017) Izzat Fahimuddin Bin Mohamed Suffian et al.   Scientific Reports

In vitro assembly of the Rous Sarcoma Virus capsid protein into hexamer tubes at physiological temperature

(2017) Soumeya A. Jaballah et al.   Scientific Reports

Enhanced assembly and colloidal stabilization of primate erythroparvovirus 1 virus-like particles for improved surface engineering

(2016) Sandra Paola Sánchez-Rodríguez et al.   Acta Biomaterialia

Self-assembly of virus-like particles of rabbit hemorrhagic disease virus capsid protein expressed in Escherichia coli and their immunogenicity in rabbits

(2016) Huimin Guo et al.   ANTIVIRAL RESEARCH

High-throughput process development of an alternative platform for the production of virus-like particles in Escherichia coli

(2016) Christopher Ladd Effio et al.   JOURNAL OF BIOTECHNOLOGY

Imaging and Quantitation of a Succession of Transient Intermediates Reveal the Reversible Self-Assembly Pathway of a Simple Icosahedral Virus Capsid

(2016) María Medrano et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Functional RNAs: combined assembly and packaging in VLPs

(2016) Po-Yu Fang et al.   NUCLEIC ACIDS RESEARCH

In vitro assembly of polymorphic virus-like particles from the capsid protein of a nodavirus

(2016) Saumya Bajaj et al.   VIROLOGY

Peptide presentation on primate erythroparvovirus 1 virus-like particles: In vitro assembly, stability and immunological properties

(2016) Areli del Carmen Morán-García et al.   VIRUS RESEARCH

Structural insights into the assembly and regulation of distinct viral capsid complexes

(2016) Subir Sarker et al.   Nature Communications

Targeted delivery system for cancer cells consist of multiple ligands conjugated genetically modified CCMV capsid on doxorubicin GNPs complex

(2016) Indu Barwal et al.   Scientific Reports

Monitoring Assembly of Virus Capsids with Nanofluidic Devices

(2015) Zachary D. Harms et al.   ACS Nano

Mechanisms of Virus Assembly

(2015) Jason D. Perlmutter et al.   Annual Review of Physical Chemistry

Engineering Virus Capsids Into Biomedical Delivery Vehicles: Structural Engineering Problems in Nanoscale

(2015) Saumya Bajaj et al.   Journal of Biomedical Nanotechnology

A Cell-Free Assembly System for Generating Infectious Human Papillomavirus 16 Capsids Implicates a Size Discrimination Mechanism for Preferential Viral Genome Packaging

(2015) Carla Cerqueira et al.   JOURNAL OF VIROLOGY

Self-assembly of virus-like particles of canine parvovirus capsid protein expressed from Escherichia coli and application as virus-like particle vaccine

(2014) Jin Xu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Hepatitis E Virus Infection

(2014) N. Kamar et al.   CLINICAL MICROBIOLOGY REVIEWS

HPV-associated diseases

(2014) Suzana Ljubojevic et al.   CLINICS IN DERMATOLOGY

Viral Particles Drive Rapid Differentiation of Memory B Cells into Secondary Plasma Cells Producing Increased Levels of Antibodies

(2014) F. Zabel et al.   JOURNAL OF IMMUNOLOGY

Self-assembly triggered by self-assembly: Optically active, paramagnetic micelles encapsulated in protein cage nanoparticles

(2014) Jealemy Galindo Millán et al.   JOURNAL OF INORGANIC BIOCHEMISTRY

Robust manufacturing and comprehensive characterization of recombinant hepatitis E virus-like particles in Hecolin®

(2014) Xiao Zhang et al.   VACCINE

Purified coronavirus spike protein nanoparticles induce coronavirus neutralizing antibodies in mice

(2014) Christopher M. Coleman et al.   VACCINE

Bioengineering virus-like particles as vaccines

(2013) Linda H.L. Lua et al.   BIOTECHNOLOGY AND BIOENGINEERING

Viral protein-coating of magnetic nanoparticles using simian virus 40 VP1

(2013) Teruya Enomoto et al.   JOURNAL OF BIOTECHNOLOGY

The Assembly Pathway of an Icosahedral Single-Stranded RNA Virus Depends on the Strength of Inter-Subunit Attractions

(2013) Rees F. Garmann et al.   JOURNAL OF MOLECULAR BIOLOGY

Norovirus Capsid Proteins Self-Assemble through Biphasic Kinetics via Long-Lived Stave-like Intermediates

(2013) Guillaume Tresset et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The Effect of Chemical Chaperones on the Assembly and Stability of HIV-1 Capsid Protein

(2013) Ayala Lampel et al.   PLoS One

Osmolyte-Mediated Encapsulation of Proteins inside MS2 Viral Capsids

(2012) Jeff E. Glasgow et al.   ACS Nano

Encapsidation of DNA, a protein and a fluorophore into virus-like particles by the capsid protein of cucumber mosaic virus

(2012) X. Lu et al.   JOURNAL OF GENERAL VIROLOGY

Construction and Characterization of Virus-Like Particles: A Review

(2012) Andris Zeltins   MOLECULAR BIOTECHNOLOGY

MS2 Viruslike Particles: A Robust, Semisynthetic Targeted Drug Delivery Platform

(2012) Francis A. Galaway et al.   MOLECULAR PHARMACEUTICS

Disassembly and reassembly improves morphology and thermal stability of human papillomavirus type 16 virus-like particles

(2012) Qinjian Zhao et al.   Nanomedicine-Nanotechnology Biology and Medicine

Hepatitis E

(2012) Jay H. Hoofnagle et al.   NEW ENGLAND JOURNAL OF MEDICINE

Respiratory Syncytial Virus Fusion Glycoprotein Expressed in Insect Cells Form Protein Nanoparticles That Induce Protective Immunity in Cotton Rats

(2012) Gale Smith et al.   PLoS One

Evidence that viral RNAs have evolved for efficient, two-stage packaging

(2012) A. Borodavka et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Safety and immunogenicity of a Sf9 insect cell-derived respiratory syncytial virus fusion protein nanoparticle vaccine

(2012) Gregory M. Glenn et al.   VACCINE

Hepatitis E vaccine development

(2012) Ting Wu et al.   Human Vaccines & Immunotherapeutics

Cell-Specific Delivery of Diverse Cargos by Bacteriophage MS2 Virus-like Particles

(2011) Carlee E. Ashley et al.   ACS Nano

Human parvovirus B19 virus-like particles: In vitro assembly and stability

(2011) Sandra P. Sánchez-Rodríguez et al.   BIOCHIMIE

Self-Assembled Virus-Like Particles from Rotavirus Structural Protein VP6 for Targeted Drug Delivery

(2011) Qinghuan Zhao et al.   BIOCONJUGATE CHEMISTRY

Rational design and optimization of downstream processes of virus particles for biopharmaceutical applications: Current advances

(2011) Tiago Vicente et al.   BIOTECHNOLOGY ADVANCES

Synthesis and application of virus-based hybrid nanomaterials

(2011) Sang-Yup Lee et al.   BIOTECHNOLOGY AND BIOENGINEERING

Efficient disulfide bond formation in virus-like particles

(2011) Bradley C. Bundy et al.   JOURNAL OF BIOTECHNOLOGY

The Bacteriophage Lambda gpNu3 Scaffolding Protein Is an Intrinsically Disordered and Biologically Functional Procapsid Assembly Catalyst

(2011) Eva Margarita Medina et al.   JOURNAL OF MOLECULAR BIOLOGY

Self-Assembly of Viral Capsid Protein and RNA Molecules of Different Sizes: Requirement for a Specific High Protein/RNA Mass Ratio

(2011) R. D. Cadena-Nava et al.   JOURNAL OF VIROLOGY

Understanding the Concentration Dependence of Viral Capsid Assembly Kinetics—the Origin of the Lag Time and Identifying the Critical Nucleus Size

(2010) Michael F. Hagan et al.   BIOPHYSICAL JOURNAL

Virus-like particles in vaccine development

(2010) António Roldão et al.   Expert Review of Vaccines

Virus-like Particles Templated by DNA Micelles: A General Method for Loading Virus Nanocarriers

(2010) Minseok Kwak et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Efficacy and safety of a recombinant hepatitis E vaccine in healthy adults: a large-scale, randomised, double-blind placebo-controlled, phase 3 trial

(2010) Feng-Cai Zhu et al.   LANCET

Norwalk Virus Assembly and Stability Monitored by Mass Spectrometry

(2010) Glen K. Shoemaker et al.   MOLECULAR & CELLULAR PROTEOMICS

Viral nanoparticles as platforms for next-generation therapeutics and imaging devices

(2010) Nicole F. Steinmetz   Nanomedicine-Nanotechnology Biology and Medicine

Interrogating viral capsid assembly with ion mobility–mass spectrometry

(2010) Charlotte Uetrecht et al.   Nature Chemistry

Subunit exchange rates in Hepatitis B virus capsids are geometry- and temperature-dependent

(2010) Charlotte Uetrecht et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

Controlled Integration of Polymers into Viral Capsids

(2009) Marta Comellas-Aragonès et al.   BIOMACROMOLECULES

Characterization of the In Vitro HIV-1 Capsid Assembly Pathway

(2009) Eric Barklis et al.   JOURNAL OF MOLECULAR BIOLOGY

Phase Diagram of Self-assembled Viral Capsid Protein Polymorphs†

(2009) L. Lavelle et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Structure of the hepatitis E virus-like particle suggests mechanisms for virus assembly and receptor binding

(2009) T. S. Y. Guu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Imaging Viral Behavior in Mammalian Cells with Self-Assembled Capsid-Quantum-Dot Hybrid Particles

(2009) Feng Li et al.   Small

Refining HPV 16 L1 purification from E. coli: Reducing endotoxin contaminations and their impact on immunogenicity

(2009) Lysann Schädlich et al.   VACCINE

The coming of age of virus-like particle vaccines

(2008) Gary T. Jennings et al.   BIOLOGICAL CHEMISTRY

Detection of Intermediates and Kinetic Control during Assembly of Bacteriophage P22 Procapsid

(2008) Roman Tuma et al.   JOURNAL OF MOLECULAR BIOLOGY

Escherichia coli-based cell-free synthesis of virus-like particles

(2007) Bradley C. Bundy et al.   BIOTECHNOLOGY AND BIOENGINEERING