CRISPR interference-guided multiplex repression of endogenous competing pathway genes for redirecting metabolic flux in Escherichia coli

Leucine zipper-mediated targeting of multi-enzyme cascade reactions to inclusion bodies in Escherichia coli for enhanced production of 1-butanol

(2017) Gui Hwan Han et al.   METABOLIC ENGINEERING

CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production

(2017) Brady F. Cress et al.   Microbial Cell Factories

Enhanced integration of large DNA intoE. colichromosome by CRISPR/Cas9

(2016) Mu-En Chung et al.   BIOTECHNOLOGY AND BIOENGINEERING

Systematic engineering of the central metabolism in Escherichia coli for effective production of n-butanol

(2016) Mukesh Saini et al.   Biotechnology for Biofuels

CRISPR interference-guided balancing of a biosynthetic mevalonate pathway increases terpenoid production

(2016) Seong Keun Kim et al.   METABOLIC ENGINEERING

CRISPRi engineering E. coli for morphology diversification

(2016) Dina Elhadi et al.   METABOLIC ENGINEERING

Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts

(2016) Jung-Hun Kim et al.   Microbial Cell Factories

Rapid and Efficient One-Step Metabolic Pathway Integration in E. coli

(2016) Marcelo C. Bassalo et al.   ACS Synthetic Biology

CRMAGE: CRISPR Optimized MAGE Recombineering

(2016) Carlotta Ronda et al.   Scientific Reports

Metabolic pathway balancing and its role in the production of biofuels and chemicals

(2015) J Andrew Jones et al.   CURRENT OPINION IN BIOTECHNOLOGY

Regulating malonyl-CoA metabolism via synthetic antisense RNAs for enhanced biosynthesis of natural products

(2015) Yaping Yang et al.   METABOLIC ENGINEERING

Metabolic engineering of Escherichia coli using CRISPR–Cas9 meditated genome editing

(2015) Yifan Li et al.   METABOLIC ENGINEERING

Application of CRISPRi for prokaryotic metabolic engineering involving multiple genes, a case study: Controllable P(3HB- co -4HB) biosynthesis

(2015) Li Lv et al.   METABOLIC ENGINEERING

Systems strategies for developing industrial microbial strains

(2015) Sang Yup Lee et al.   NATURE BIOTECHNOLOGY

Enhancing flavonoid production by systematically tuning the central metabolic pathways based on a CRISPR interference system in Escherichia coli

(2015) Junjun Wu et al.   Scientific Reports

CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR Arrays for dCas9-Mediated Multiplex Transcriptional Repression in E. coli

(2015) Brady F. Cress et al.   ACS Synthetic Biology

Improving poly-3-hydroxybutyrate production in Escherichia coli by combining the increase in the NADPH pool and acetyl-CoA availability

(2014) Sara Centeno-Leija et al.   ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY

Fine-Tuning of the Fatty Acid Pathway by Synthetic Antisense RNA for Enhanced (2S)-Naringenin Production from l-Tyrosine in Escherichia coli

(2014) Junjun Wu et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Expanding ester biosynthesis in Escherichia coli

(2014) Gabriel M Rodriguez et al.   Nature Chemical Biology

Rapid editing and evolution of bacterial genomes using libraries of synthetic DNA

(2014) Ryan R Gallagher et al.   Nature Protocols

SEVA 2.0: an update of the Standard European Vector Architecture for de-/re-construction of bacterial functionalities

(2014) Esteban Martínez-García et al.   NUCLEIC ACIDS RESEARCH

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

Production of advanced biofuels in engineered E. coli

(2013) Miao Wen et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production

(2013) Jorge Alonso-Gutierrez et al.   METABOLIC ENGINEERING

Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs

(2013) Dokyun Na et al.   NATURE BIOTECHNOLOGY

Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli

(2013) Seung Min Yoo et al.   Nature Protocols

Spanning high-dimensional expression space using ribosome-binding site combinatorics

(2013) Lior Zelcbuch et al.   NUCLEIC ACIDS RESEARCH

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system

(2013) David Bikard et al.   NUCLEIC ACIDS RESEARCH

Modular optimization of multi-gene pathways for fatty acids production in E. coli

(2013) Peng Xu et al.   Nature Communications

A dynamic metabolite valve for the control of central carbon metabolism

(2012) Kevin V. Solomon et al.   METABOLIC ENGINEERING

Customized optimization of metabolic pathways by combinatorial transcriptional engineering

(2012) Jing Du et al.   NUCLEIC ACIDS RESEARCH

Driving Forces Enable High-Titer Anaerobic 1-Butanol Synthesis in Escherichia coli

(2011) Claire R. Shen et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Engineering Artificial Small RNAs for Conditional Gene Silencing in Escherichia coli

(2011) Vandana Sharma et al.   ACS Synthetic Biology

Acetate accumulation through alternative metabolic pathways in ackA − pta − poxB − triple mutant in E. coli B (BL21)

(2010) Je-Nie Phue et al.   BIOTECHNOLOGY LETTERS

An insight into the role of phosphotransacetylase (pta) and the acetate/acetyl-CoA node in Escherichia coli

(2009) Sara Castaño-Cerezo et al.   Microbial Cell Factories

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations

(2008) Angelika Wegerer et al.   BMC BIOTECHNOLOGY

Metabolic engineering of Escherichia coli for 1-butanol production

(2007) Shota Atsumi et al.   METABOLIC ENGINEERING

Characterization of the Acetate-Producing Pathways in Escherichia coli

(2005) Cheryl R. Dittrich et al.   BIOTECHNOLOGY PROGRESS