Directed evolution for high functional production and stability of a challenging G protein-coupled receptor
Published 2021 View Full Article
- Home
- Publications
- Publication Search
- Publication Details
Title
Directed evolution for high functional production and stability of a challenging G protein-coupled receptor
Authors
Keywords
-
Journal
Scientific Reports
Volume 11, Issue 1, Pages -
Publisher
Springer Science and Business Media LLC
Online
2021-04-21
DOI
10.1038/s41598-021-87793-9
References
Ask authors/readers for more resources
Related references
Note: Only part of the references are listed.- Optimizing the α1B-adrenergic receptor for solution NMR studies
- (2020) Matthias Schuster et al. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
- Production of membrane proteins in industry: The example of GPCRs
- (2020) James C. Errey et al. PROTEIN EXPRESSION AND PURIFICATION
- Crystal structure of the human oxytocin receptor
- (2020) Yann Waltenspühl et al. Science Advances
- MultiBac: Baculovirus-Mediated Multigene DNA Cargo Delivery in Insect and Mammalian Cells
- (2019) Kapil Gupta et al. Viruses-Basel
- Determining protein structures using deep mutagenesis
- (2019) Jörn M. Schmiedel et al. NATURE GENETICS
- Accurate circular consensus long-read sequencing improves variant detection and assembly of a human genome
- (2019) Aaron M. Wenger et al. NATURE BIOTECHNOLOGY
- Elucidating the active δ-opioid receptor crystal structure with peptide and small-molecule agonists
- (2019) Tobias Claff et al. Science Advances
- Recent advances in the determination of G protein-coupled receptor structures
- (2018) David M Thal et al. CURRENT OPINION IN STRUCTURAL BIOLOGY
- Enabling STD-NMR fragment screening using stabilized native GPCR: A case study of adenosine receptor
- (2018) Sébastien Igonet et al. Scientific Reports
- High-resolution crystal structure of parathyroid hormone 1 receptor in complex with a peptide agonist
- (2018) Janosch Ehrenmann et al. NATURE STRUCTURAL & MOLECULAR BIOLOGY
- Trends in GPCR drug discovery: new agents, targets and indications
- (2017) Alexander S. Hauser et al. NATURE REVIEWS DRUG DISCOVERY
- New approaches towards the understanding of integral membrane proteins: A structural perspective on G protein-coupled receptors
- (2017) Reinhard Grisshammer PROTEIN SCIENCE
- SPR-based fragment screening with neurotensin receptor 1 generates novel small molecule ligands
- (2017) Sylwia Huber et al. PLoS One
- Covalently circularized nanodiscs for studying membrane proteins and viral entry
- (2016) Mahmoud L Nasr et al. NATURE METHODS
- Conformational dynamics of a G-protein α subunit is tightly regulated by nucleotide binding
- (2016) David Goricanec et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Directed evolution of G protein-coupled receptors in yeast for higher functional production in eukaryotic expression hosts
- (2016) Marco Schütz et al. Scientific Reports
- A generic selection system for improved expression and thermostability of G protein-coupled receptors by directed evolution
- (2016) Christoph Klenk et al. Scientific Reports
- PacBio Sequencing and Its Applications
- (2015) Anthony Rhoads et al. GENOMICS PROTEOMICS & BIOINFORMATICS
- Improving the apo-state detergent stability of NTS1 with CHESS for pharmacological and structural studies
- (2014) Daniel J. Scott et al. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
- Structure of signaling-competent neurotensin receptor 1 obtained by directed evolution inEscherichia coli
- (2014) Pascal Egloff et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- The Resolution Revolution
- (2014) W. Kuhlbrandt SCIENCE
- Stabilizing membrane proteins through protein engineering
- (2013) Daniel J Scott et al. CURRENT OPINION IN CHEMICAL BIOLOGY
- Production of GPCR and GPCR complexes for structure determination
- (2013) Shoji Maeda et al. CURRENT OPINION IN STRUCTURAL BIOLOGY
- Structure-Function of the G Protein–Coupled Receptor Superfamily
- (2012) Vsevolod Katritch et al. Annual Review of Pharmacology and Toxicology
- Maximizing Detergent Stability and Functional Expression of a GPCR by Exhaustive Recombination and Evolution
- (2012) Karola M. Schlinkmann et al. JOURNAL OF MOLECULAR BIOLOGY
- Direct Molecular Evolution of Detergent-Stable G Protein-Coupled Receptors Using Polymer Encapsulated Cells
- (2012) Daniel J. Scott et al. JOURNAL OF MOLECULAR BIOLOGY
- Critical features for biosynthesis, stability, and functionality of a G protein-coupled receptor uncovered by all-versus-all mutations
- (2012) K. M. Schlinkmann et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Current trends in α-helical membrane protein crystallization: An update
- (2012) Joanne L. Parker et al. PROTEIN SCIENCE
- Fusion Partner Toolchest for the Stabilization and Crystallization of G Protein-Coupled Receptors
- (2012) Eugene Chun et al. STRUCTURE
- Evolution of Three Human GPCRs for Higher Expression and Stability
- (2011) Igor Dodevski et al. JOURNAL OF MOLECULAR BIOLOGY
- Overcoming barriers to membrane protein structure determination
- (2011) Roslyn M Bill et al. NATURE BIOTECHNOLOGY
- Intranasal oxytocin reduces psychotic symptoms and improves Theory of Mind and social perception in schizophrenia
- (2011) Cort A. Pedersen et al. SCHIZOPHRENIA RESEARCH
- Crystallizing membrane proteins using lipidic mesophases
- (2009) Martin Caffrey et al. Nature Protocols
- Fluorescent Agonists and Antagonists for Vasopressin/Oxytocin G Protein-Coupled Receptors: Usefulness in Ligand Screening Assays and Receptor Studies
- (2008) B. Mouillac et al. MINI-REVIEWS IN MEDICINAL CHEMISTRY
- Structural diversity of G protein-coupled receptors and significance for drug discovery
- (2008) Malin C. Lagerström et al. NATURE REVIEWS DRUG DISCOVERY
- Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity
- (2008) C. A. Sarkar et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Co-evolving stability and conformational homogeneity of the human adenosine A2a receptor
- (2008) F. Magnani et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Conformational thermostabilization of the 1-adrenergic receptor in a detergent-resistant form
- (2008) M. J. Serrano-Vega et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Oxytocin, Vasopressin, and the Neurogenetics of Sociality
- (2008) Z. R. Donaldson et al. SCIENCE
- Microscale Fluorescent Thermal Stability Assay for Membrane Proteins
- (2008) Alexander I. Alexandrov et al. STRUCTURE
Discover Peeref hubs
Discuss science. Find collaborators. Network.
Join a conversationCreate your own webinar
Interested in hosting your own webinar? Check the schedule and propose your idea to the Peeref Content Team.
Create Now