MultiBac: Baculovirus-Mediated Multigene DNA Cargo Delivery in Insect and Mammalian Cells

An online resource for GPCR structure determination and analysis

(2019) Christian Munk et al.   NATURE METHODS

In vivo assembly and large-scale purification of a GPCR - Gα fusion with Gβγ, and characterization of the active complex

(2019) Abhinav Kumar et al.   PLoS One

Recent advances in the determination of G protein-coupled receptor structures

(2018) David M Thal et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Homozygous TAF8 mutation in a patient with intellectual disability results in undetectable TAF8 protein, but preserved RNA polymerase II transcription

(2018) Farrah El-Saafin et al.   HUMAN MOLECULAR GENETICS

Baculovirus-driven protein expression in insect cells: A benchmarking study

(2018) Peggy Stolt-Bergner et al.   JOURNAL OF STRUCTURAL BIOLOGY

Baculovirus for gene delivery to mammalian cells: Past, present and future

(2018) Maysam Mansouri et al.   PLASMID

Baculovirus Surface Display of Immunogenic Proteins for Vaccine Development

(2018) Balraj Premanand et al.   Viruses-Basel

Structural basis for GPR40 allosteric agonism and incretin stimulation

(2018) Joseph D. Ho et al.   Nature Communications

MultiBacMam Bimolecular Fluorescence Complementation (BiFC) tool-kit identifies new small-molecule inhibitors of the CDK5-p25 protein-protein interaction (PPI)

(2018) Itxaso Bellón-Echeverría et al.   Scientific Reports

Structure of activated transcription complex Pol II–DSIF–PAF–SPT6

(2018) Seychelle M. Vos et al.   NATURE

Structural basis for ATP-dependent chromatin remodelling by the INO80 complex

(2018) Sebastian Eustermann et al.   NATURE

Structure and regulation of the human INO80–nucleosome complex

(2018) Rafael Ayala et al.   NATURE

The nuclear actin-containing Arp8 module is a linker DNA sensor driving INO80 chromatin remodeling

(2018) Kilian R. Knoll et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Baculovirus as a Tool for Gene Delivery and Gene Therapy

(2018) Chikako Ono et al.   Viruses-Basel

GPCR drug discovery: integrating solution NMR data with crystal and cryo-EM structures

(2018) Ichio Shimada et al.   NATURE REVIEWS DRUG DISCOVERY

Building transcription complexes

(2018) Alan C. M. Cheung   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Chaperonin CCT checkpoint function in basal transcription factor TFIID assembly

(2018) Simona V. Antonova et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

High-resolution crystal structure of parathyroid hormone 1 receptor in complex with a peptide agonist

(2018) Janosch Ehrenmann et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Influenza vaccine: Where are we and where do we go?

(2018) Mohsen Keshavarz et al.   REVIEWS IN MEDICAL VIROLOGY

Structure and dynamics of the yeast SWR1-nucleosome complex

(2018) Oliver Willhoft et al.   SCIENCE

Crystal structures of the human neurokinin 1 receptor in complex with clinically used antagonists

(2018) Jendrik Schöppe et al.   Nature Communications

Molecular mechanism for the regulation of yeast separase by securin

(2017) Shukun Luo et al.   NATURE

Trends in GPCR drug discovery: new agents, targets and indications

(2017) Alexander S. Hauser et al.   NATURE REVIEWS DRUG DISCOVERY

Structural basis of the specificity of USP18 toward ISG15

(2017) Anja Basters et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Cryo-EM structures of the human INO80 chromatin-remodeling complex

(2017) Ricardo J. Aramayo et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Molecular basis for the recognition of the human AAUAAA polyadenylation signal

(2017) Yadong Sun et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Close Encounters – Probing Proximal Proteins in Live or Fixed Cells

(2017) Peter Lönn et al.   TRENDS IN BIOCHEMICAL SCIENCES

Structure of the cohesin loader Scc2

(2017) William C. H. Chao et al.   Nature Communications

The vaccinia virus DNA polymerase structure provides insights into the mode of processivity factor binding

(2017) Nicolas Tarbouriech et al.   Nature Communications

Structural basis of the specificity of USP18 toward ISG15

(2017) Anja Basters et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Cryo-EM structures of the human INO80 chromatin-remodeling complex

(2017) Ricardo J. Aramayo et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Architecture of TAF11/TAF13/TBP complex suggests novel regulation properties of general transcription factor TFIID

(2017) Kapil Gupta et al.   eLife

Decoding the centromeric nucleosome through CENP-N

(2017) Satyakrishna Pentakota et al.   eLife

Neuroprotective Mechanisms Mediated by CDK5 Inhibition

(2016) Gohar Mushtaq et al.   CURRENT PHARMACEUTICAL DESIGN

Progress in developing virus-like particle influenza vaccines

(2016) Fu-Shi Quan et al.   Expert Review of Vaccines

Zooming in on Transcription Preinitiation

(2016) Kapil Gupta et al.   JOURNAL OF MOLECULAR BIOLOGY

Recombinant expression and reconstitution of multiprotein complexes by the USER cloning method in the insect cell-baculovirus expression system

(2016) Ziguo Zhang et al.   METHODS

Genetic code expansion for multiprotein complex engineering

(2016) Christine Koehler et al.   NATURE METHODS

biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes

(2016) Florian Weissmann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Immune suppressive activity of the influenza fusion peptide

(2016) Shervin Bahrami et al.   VIRUS RESEARCH

Highly efficient baculovirus-mediated multigene delivery in primary cells

(2016) Maysam Mansouri et al.   Nature Communications

Polyproteins in structural biology

(2015) Thibaut Crépin et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Bimolecular Fluorescence Complementation (BiFC) Analysis: Advances and Recent Applications for Genome-Wide Interaction Studies

(2015) Kristi E. Miller et al.   JOURNAL OF MOLECULAR BIOLOGY

Comprehensive analysis of heterotrimeric G-protein complex diversity and their interactions with GPCRs in solution

(2015) Matthias Hillenbrand et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cytoplasmic TAF2–TAF8–TAF10 complex provides evidence for nuclear holo–TFIID assembly from preformed submodules

(2015) Simon Trowitzsch et al.   Nature Communications

Thirty years of baculovirus-insect cell protein expression: from dark horse to mainstream technology

(2014) M. M. van Oers et al.   JOURNAL OF GENERAL VIROLOGY

The architecture of human general transcription factor TFIID core complex

(2013) Christoph Bieniossek et al.   NATURE

Molecular signatures of G-protein-coupled receptors

(2013) A. J. Venkatakrishnan et al.   NATURE

The MultiBac Protein Complex Production Platform at the EMBL

(2013) Imre Berger et al.   Jove-Journal of Visualized Experiments

Gene gymnastics

(2013) Lakshmi S Vijayachandran et al.   Bioengineered

Structure-Function of the G Protein–Coupled Receptor Superfamily

(2012) Vsevolod Katritch et al.   Annual Review of Pharmacology and Toxicology

Deregulated Cdk5 Activity Is Involved in Inducing Alzheimer’s Disease

(2012) Varsha Shukla et al.   ARCHIVES OF MEDICAL RESEARCH

MultiBac complexomics

(2012) Simon Trowitzsch et al.   Expert Review of Proteomics

MultiBac turns sweet

(2012) Dieter Palmberger et al.   Bioengineered

Light it up: Highly efficient multigene delivery in mammalian cells

(2011) Simon Trowitzsch et al.   BIOESSAYS

Robots, pipelines, polyproteins: Enabling multiprotein expression in prokaryotic and eukaryotic cells

(2011) Lakshmi Sumitra Vijayachandran et al.   JOURNAL OF STRUCTURAL BIOLOGY

MultiBac: expanding the research toolbox for multiprotein complexes

(2011) Christoph Bieniossek et al.   TRENDS IN BIOCHEMICAL SCIENCES

Virus-like particles in vaccine development

(2010) António Roldão et al.   Expert Review of Vaccines

New baculovirus expression tools for recombinant protein complex production

(2010) Simon Trowitzsch et al.   JOURNAL OF STRUCTURAL BIOLOGY

Getting a Grip on Complexes

(2009) Yan Nie et al.   CURRENT GENOMICS

Automated unrestricted multigene recombineering for multiprotein complex production

(2009) Christoph Bieniossek et al.   NATURE METHODS