Journal
SCIENCE ADVANCES
Volume 5, Issue 11, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.aax9115
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Funding
- Shanghai Municipal Government
- Research Foundation Flanders (FWO Vlaanderen)
- Research Council of the Vrije Universiteit Brussel
- NIH [R33DA038858, P01DA035764]
- Canadian Institute of Health Research (CIHR) [MOP-123399, MOP-136871]
- U.S. NIH
- ShanghaiTech University
- GPCR Consortium
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Selective activation of the.-opioid receptor (DOP) has great potential for the treatment of chronic pain, benefitting from ancillary anxiolytic and antidepressant-like effects. Moreover, DOP agonists show reduced adverse effects as compared to mu-opioid receptor (MOP) agonists that are in the spotlight of the current opioid crisis. Here, we report the first crystal structures of the DOP in an activated state, in complex with two relevant and structurally diverse agonists: the potent opioid agonist peptide KGCHM07 and the small-molecule agonist DPI-287 at 2.8 and 3.3 angstrom resolution, respectively. Our study identifies key determinants for agonist recognition, receptor activation, and DOP selectivity, revealing crucial differences between both agonist scaffolds. Our findings provide the first investigation into atomic-scale agonist binding at the DOP, supported by site-directed mutagenesis and pharmacological characterization. These structures will underpin the future structure-based development of DOP agonists for an improved pain treatment with fewer adverse effects.
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