Directed evolution of G protein-coupled receptors in yeast for higher functional production in eukaryotic expression hosts

Improving the apo-state detergent stability of NTS1 with CHESS for pharmacological and structural studies

(2014) Daniel J. Scott et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Structure of signaling-competent neurotensin receptor 1 obtained by directed evolution inEscherichia coli

(2014) Pascal Egloff et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

An overview of the diverse roles of G-protein coupled receptors (GPCRs) in the pathophysiology of various human diseases

(2013) Boon Chin Heng et al.   BIOTECHNOLOGY ADVANCES

Production of GPCR and GPCR complexes for structure determination

(2013) Shoji Maeda et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Maximizing Detergent Stability and Functional Expression of a GPCR by Exhaustive Recombination and Evolution

(2012) Karola M. Schlinkmann et al.   JOURNAL OF MOLECULAR BIOLOGY

Direct Molecular Evolution of Detergent-Stable G Protein-Coupled Receptors Using Polymer Encapsulated Cells

(2012) Daniel J. Scott et al.   JOURNAL OF MOLECULAR BIOLOGY

Protein selection using yeast surface display

(2012) Nimish Gera et al.   METHODS

Platform for the rapid construction and evaluation of GPCRs for crystallography in Saccharomyces cerevisiae

(2012) Mitsunori Shiroishi et al.   Microbial Cell Factories

Critical features for biosynthesis, stability, and functionality of a G protein-coupled receptor uncovered by all-versus-all mutations

(2012) K. M. Schlinkmann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Lipidic cubic phase technologies for membrane protein structural studies

(2011) Vadim Cherezov   CURRENT OPINION IN STRUCTURAL BIOLOGY

Evolution of Three Human GPCRs for Higher Expression and Stability

(2011) Igor Dodevski et al.   JOURNAL OF MOLECULAR BIOLOGY

Crystal structure of the β2 adrenergic receptor–Gs protein complex

(2011) Søren G. F. Rasmussen et al.   NATURE

MultiBac: expanding the research toolbox for multiprotein complexes

(2011) Christoph Bieniossek et al.   TRENDS IN BIOCHEMICAL SCIENCES

An improved method for whole protein extraction from yeast Saccharomyces cerevisiae

(2011) Tingting Zhang et al.   YEAST

New baculovirus expression tools for recombinant protein complex production

(2010) Simon Trowitzsch et al.   JOURNAL OF STRUCTURAL BIOLOGY

Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield

(2009) Nicklas Bonander et al.   Microbial Cell Factories

Progress toward heterologous expression of active G-protein-coupled receptors inSaccharomyces cerevisiae: Linking cellular stress response with translocation and trafficking

(2009) Michelle A. O'Malley et al.   PROTEIN SCIENCE

Crystallizing Membrane Proteins for Structure Determination: Use of Lipidic Mesophases

(2008) Martin Caffrey   Annual Review of Biophysics

A Decade of Yeast Surface Display Technology: Where Are We Now?

(2008) Eric Shusta et al.   COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING

Structural diversity of G protein-coupled receptors and significance for drug discovery

(2008) Malin C. Lagerström et al.   NATURE REVIEWS DRUG DISCOVERY

Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity

(2008) C. A. Sarkar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA