Current development in genetic engineering strategies of Bacillus species

Generation of biologically contained, readily transformable, and genetically manageable mutants of the biotechnologically important Bacillus pumilus

(2013) Stephanie Wemhoff et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Developing Bacillus spp. as a cell factory for production of microbial enzymes and industrially important biochemicals in the context of systems and synthetic biology

(2013) Long Liu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Size unlimited markerless deletions by a transconjugative plasmid-system in Bacillus licheniformis

(2013) Michael Rachinger et al.   JOURNAL OF BIOTECHNOLOGY

A versatile mini-mazF-cassette for marker-free targeted genetic modification in Bacillus subtilis

(2013) Zhiwei Lin et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Rewriting a genome

(2013) Emmanuelle Charpentier et al.   NATURE

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Establishment of a Markerless Mutation Delivery System in Bacillus subtilis Stimulated by a Double-Strand Break in the Chromosome

(2013) Ting Shi et al.   PLoS One

Proteomic analysis ofBacillus subtilisstrains engineered for improved production of heterologous proteins

(2013) Susanne Pohl et al.   PROTEOMICS

Toxicity of indoxyl derivative accumulation in bacteria and its use as a new counterselection principle

(2013) Angel Angelov et al.   SYSTEMATIC AND APPLIED MICROBIOLOGY

Counterselection System for Geobacillus kaustophilus HTA426 through Disruption ofpyrFandpyrR

(2012) Hirokazu Suzuki et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Bacillus subtilis genome editing using ssDNA with short homology regions

(2012) Yang Wang et al.   NUCLEIC ACIDS RESEARCH

A new method for multiple gene inactivations in Bacillus subtilis 168, producing a strain free of selectable markers

(2011) Chong Zhang et al.   CANADIAN JOURNAL OF MICROBIOLOGY

Systems biology of yeast: enabling technology for development of cell factories for production of advanced biofuels

(2011) Bouke de Jong et al.   CURRENT OPINION IN BIOTECHNOLOGY

Genome sequence of B. amyloliquefaciens type strain DSM7T reveals differences to plant-associated B. amyloliquefaciens FZB42

(2011) Christian Rückert et al.   JOURNAL OF BIOTECHNOLOGY

Regulation of mtl operon promoter of Bacillus subtilis: requirements of its use in expression vectors

(2011) Kambiz Morabbi Heravi et al.   Microbial Cell Factories

Genetic Tool Development for a New Host for Biotechnology, the Thermotolerant Bacterium Bacillus coagulans

(2010) A. T. Kovacs et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

An improved transconjugation protocol for Bacillus megaterium facilitating a direct genetic knockout

(2010) Janine Richhardt et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Single-stranded heteroduplex intermediates in λ Red homologous recombination

(2010) Marcello Maresca et al.   BMC MOLECULAR BIOLOGY

A new simple method to introduce marker-free deletions in the Bacillus subtilis genome

(2010) Takuya Morimoto et al.   GENES & GENETIC SYSTEMS

Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides

(2010) Joseph R Warner et al.   NATURE BIOTECHNOLOGY

Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli

(2010) P. K. Ajikumar et al.   SCIENCE

mazFas a counter-selectable marker for unmarked genetic modification ofPichia pastoris

(2009) Junjie Yang et al.   FEMS YEAST RESEARCH

In vivo Activation of Tetracycline Rep ressor by Cre/lox-Mediated Gene Assembly

(2009) Ralph Bertram et al.   JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Cre/lox System and PCR-Based Genome Engineering in Bacillus subtilis

(2008) X. Yan et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Plasmid pCS1966, a New Selection/Counterselection Tool for Lactic Acid Bacterium Strain Construction Based on the oroP Gene, Encoding an Orotate Transporter from Lactococcus lactis

(2008) C. Solem et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Enhanced Recombinant Protein Productivity by Genome Reduction in Bacillus subtilis

(2008) T. Morimoto et al.   DNA RESEARCH