CRISPR-based detection of SARS-CoV-2: A review from sample to result

Universal and Naked-Eye Gene Detection Platform Based on the Clustered Regularly Interspaced Short Palindromic Repeats/Cas12a/13a System

(2020) Chaoqun Yuan et al.   ANALYTICAL CHEMISTRY

Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

(2020) Victor M Corman et al.   Eurosurveillance

The rapidly advancing Class 2 CRISPR‐Cas technologies: A customizable toolbox for molecular manipulations

(2020) Jingyi Wang et al.   JOURNAL OF CELLULAR AND MOLECULAR MEDICINE

Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding

(2020) Roujian Lu et al.   LANCET

Viral load of SARS-CoV-2 in clinical samples

(2020) Yang Pan et al.   LANCET INFECTIOUS DISEASES

A Novel Coronavirus from Patients with Pneumonia in China, 2019

(2020) Na Zhu et al.   NEW ENGLAND JOURNAL OF MEDICINE

Early Transmission Dynamics in Wuhan, China, of Novel Coronavirus–Infected Pneumonia

(2020) Qun Li et al.   NEW ENGLAND JOURNAL OF MEDICINE

High-throughput and all-solution phase African Swine Fever Virus (ASFV) detection using CRISPR-Cas12a and fluorescence based point-of-care system

(2020) Qian He et al.   BIOSENSORS & BIOELECTRONICS

The Architecture of SARS-CoV-2 Transcriptome

(2020) Dongwan Kim et al.   CELL

Novel coronavirus: From discovery to clinical diagnostics

(2020) Tung Phan   INFECTION GENETICS AND EVOLUTION

Saliva as an Alternate Specimen Source for Detection of SARS-CoV-2 in Symptomatic Patients Using Cepheid Xpert Xpress SARS-CoV-2

(2020) Clare McCormick-Baw et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Saliva as a non-invasive specimen for detection of SARS-CoV-2

(2020) Eloise Williams et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Comparative Performance of SARS-CoV-2 Detection Assays Using Seven Different Primer-Probe Sets and One Assay Kit

(2020) Arun K. Nalla et al.   JOURNAL OF CLINICAL MICROBIOLOGY

CRISPR–Cas12-based detection of SARS-CoV-2

(2020) James P. Broughton et al.   NATURE BIOTECHNOLOGY

Nucleic Acid Detection Using CRISPR/Cas Biosensing Technologies

(2020) Rashid Aman et al.   ACS Synthetic Biology

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Children and Adolescents

(2020) Riccardo Castagnoli et al.   JAMA Pediatrics

Sequence-Specific Recognition of HIV-1 DNA with Solid-State CRISPR-Cas12a-Assisted Nanopores (SCAN)

(2020) Reza Nouri et al.   ACS Sensors

SARS-CoV-2 detection with CRISPR diagnostics

(2020) Lu Guo et al.   Cell Discovery

A scoutRNA Is Required for Some Type V CRISPR-Cas Systems

(2020) Lucas B. Harrington et al.   MOLECULAR CELL

Futuristic CRISPR-based biosensing in the cloud and internet of things era: an overview

(2020) Abdullahi Umar Ibrahim et al.   MULTIMEDIA TOOLS AND APPLICATIONS

Evaluation and improvement of isothermal amplification methods for point-of-need plant disease diagnostics

(2020) Yiping Zou et al.   PLoS One

Point-of-care CRISPR/Cas nucleic acid detection: Recent advances, challenges and opportunities

(2020) Jeanne E. van Dongen et al.   BIOSENSORS & BIOELECTRONICS

A lateral flow strip combined with Cas9 nickase-triggered amplification reaction for dual food-borne pathogen detection

(2020) Luying Wang et al.   BIOSENSORS & BIOELECTRONICS

CRISPR/cas systems redefine nucleic acid detection: Principles and methods

(2020) Meng Wang et al.   BIOSENSORS & BIOELECTRONICS

Diagnostics for SARS-CoV-2 detection: A comprehensive review of the FDA-EUA COVID-19 testing landscape

(2020) Neeraja Ravi et al.   BIOSENSORS & BIOELECTRONICS

Extraction-free SARS-CoV-2 detection by rapid RT-qPCR universal for all primary respiratory materials

(2020) Nadine Lübke et al.   JOURNAL OF CLINICAL VIROLOGY

The implementation of a rapid sample preparation method for the detection of SARS-CoV-2 in a diagnostic laboratory in South Africa

(2020) Gert Marais et al.   PLoS One

SARS-CoV-2 detection using isothermal amplification and a rapid, inexpensive protocol for sample inactivation and purification

(2020) Brian A. Rabe et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

iSCAN: An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive detection of SARS-CoV-2

(2020) Zahir Ali et al.   VIRUS RESEARCH

Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay

(2020) Xiong Ding et al.   Nature Communications

A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR

(2020) Aniela Wozniak et al.   Scientific Reports

Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA

(2020) Maturada Patchsung et al.   Nature Biomedical Engineering

ZnAl nano layered double hydroxides for dual functional CRISPR/Cas9 delivery and enhanced green fluorescence protein biosensor

(2020) Navid Rabiee et al.   Scientific Reports

CRISPR/Cas Systems towards Next-Generation Biosensing

(2019) Yi Li et al.   TRENDS IN BIOTECHNOLOGY

Engineering of CRISPR-Cas12b for human genome editing

(2019) Jonathan Strecker et al.   Nature Communications

Rapid and Fully Microfluidic Ebola Virus Detection with CRISPR-Cas13a

(2019) Peiwu Qin et al.   ACS Sensors

Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing

(2019) Benjamin P. Kleinstiver et al.   NATURE BIOTECHNOLOGY

Detection of unamplified target genes via CRISPR–Cas9 immobilized on a graphene field-effect transistor

(2019) Reza Hajian et al.   Nature Biomedical Engineering

CasX: a new and small CRISPR gene-editing protein

(2019) Hui Yang et al.   CELL RESEARCH

The next generation of CRISPR–Cas technologies and applications

(2019) Adrian Pickar-Oliver et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

CRISPR/Cas Multiplexed Biosensing: A Challenge or an Insurmountable Obstacle?

(2019) Yi Li et al.   TRENDS IN BIOTECHNOLOGY

Cas12aVDet: A CRISPR/Cas12a-Based Platform for Rapid and Visual Nucleic Acid Detection

(2019) Bei Wang et al.   ANALYTICAL CHEMISTRY

CRISPR-Cas12a Coupled with Platinum Nanoreporter for Visual Quantification of SNVs on a Volumetric Bar-Chart Chip

(2019) Ning Shao et al.   ANALYTICAL CHEMISTRY

SHERLOCK: nucleic acid detection with CRISPR nucleases

(2019) Max J. Kellner et al.   Nature Protocols

Visual detection of porcine reproductive and respiratory syndrome virus using CRISPR‐Cas13a

(2019) Yafei Chang et al.   Transboundary and Emerging Diseases

HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation

(2019) Linxian Li et al.   ACS Synthetic Biology

CRISPR/Cas13a‐Powered Electrochemical Microfluidic Biosensor for Nucleic Acid Amplification‐Free miRNA Diagnostics

(2019) Richard Bruch et al.   ADVANCED MATERIALS

Exploring the Trans-Cleavage Activity of CRISPR-Cas12a (cpf1) for the Development of a Universal Electrochemical Biosensor

(2019) Yifan Dai et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A low-cost fluorescence reader for in vitro transcription and nucleic acid detection with Cas13a

(2019) Florian Katzmeier et al.   PLoS One

Cas12a-Based On-Site and Rapid Nucleic Acid Detection of African Swine Fever

(2019) Jing Bai et al.   Frontiers in Microbiology

Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors

(2018) Silvana Konermann et al.   CELL

CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA

(2018) Shi-Yuan Li et al.   CELL RESEARCH

History of CRISPR-Cas from Encounter with a Mysterious Repeated Sequence to Genome Editing Technology

(2018) Yoshizumi Ishino et al.   JOURNAL OF BACTERIOLOGY

Cas13d Is a Compact RNA-Targeting Type VI CRISPR Effector Positively Modulated by a WYL-Domain-Containing Accessory Protein

(2018) Winston X. Yan et al.   MOLECULAR CELL

Field-deployable viral diagnostics using CRISPR-Cas13

(2018) Cameron Myhrvold et al.   SCIENCE

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

(2018) Jonathan S. Gootenberg et al.   SCIENCE

CRISPR/dCas9-mediated biosensor for detection of tick-borne diseases

(2018) Bonhan Koo et al.   SENSORS AND ACTUATORS B-CHEMICAL

CRISPR–Cas13a: Prospects for Plant Virus Resistance

(2018) Muhammad Zuhaib Khan et al.   TRENDS IN BIOTECHNOLOGY

Synthesis of Molecularly Imprinted Polymer Nanoparticles for α-Casein Detection Using Surface Plasmon Resonance as a Milk Allergen Sensor

(2018) Jon Ashley et al.   ACS Sensors

CRISPR-Cas12a-assisted nucleic acid detection

(2018) Shi-Yuan Li et al.   Cell Discovery

Detection of CRISPR-dCas9 on DNA with Solid-State Nanopores

(2018) Wayne Yang et al.   NANO LETTERS

dCas9-mediated Nanoelectrokinetic Direct Detection of Target Gene for Liquid Biopsy

(2018) Hyomin Lee et al.   NANO LETTERS

Functionally diverse type V CRISPR-Cas systems

(2018) Winston X. Yan et al.   SCIENCE

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes

(2018) Lucas B. Harrington et al.   SCIENCE

The Revolution Continues: Newly Discovered Systems Expand the CRISPR-Cas Toolkit

(2017) Karthik Murugan et al.   MOLECULAR CELL

C2c1-sgRNA Complex Structure Reveals RNA-Guided DNA Cleavage Mechanism

(2017) Liang Liu et al.   MOLECULAR CELL

Cas13b Is a Type VI-B CRISPR-Associated RNA-Guided RNase Differentially Regulated by Accessory Proteins Csx27 and Csx28

(2017) Aaron A. Smargon et al.   MOLECULAR CELL

Diversity and evolution of class 2 CRISPR–Cas systems

(2017) Sergey Shmakov et al.   NATURE REVIEWS MICROBIOLOGY

CUT-PCR: CRISPR-mediated, ultrasensitive detection of target DNA using PCR

(2017) S H Lee et al.   ONCOGENE

RNA editing with CRISPR-Cas13

(2017) David B. T. Cox et al.   SCIENCE

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Multiplexed Point-of-Care Testing – xPOCT

(2017) Can Dincer et al.   TRENDS IN BIOTECHNOLOGY

Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components

(2016) Keith Pardee et al.   CELL

New CRISPR–Cas systems from uncultivated microbes

(2016) David Burstein et al.   NATURE

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

(2016) Omar O. Abudayyeh et al.   SCIENCE

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

CRISPR-Cas Systems: Prokaryotes Upgrade to Adaptive Immunity

(2014) Rodolphe Barrangou et al.   MOLECULAR CELL

Equipment-Free Incubation of Recombinase Polymerase Amplification Reactions Using Body Heat

(2014) Zachary Austin Crannell et al.   PLoS One

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

Development of a Panel of Recombinase Polymerase Amplification Assays for Detection of Biothreat Agents

(2013) M. Euler et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

CRISPR/Cas System and Its Role in Phage-Bacteria Interactions

(2010) Hélène Deveau et al.   Annual Review of Microbiology

CRISPR/Cas, the Immune System of Bacteria and Archaea

(2010) P. Horvath et al.   SCIENCE