Engineering organophosphate hydrolase for enhanced biocatalytic performance: A review

Molecular binding scaffolds increase local substrate concentration enhancing the enzymatic hydrolysis of VX nerve agent

(2020) Xuye Lang et al.   BIOTECHNOLOGY AND BIOENGINEERING

Nanoscavenger provides long-term prophylactic protection against nerve agents in rodents

(2019) Peng Zhang et al.   Science Translational Medicine

Overcoming the Challenges of Enzyme Evolution to Adapt Phosphotriesterase for V-agent Decontamination

(2019) Andrew N. Bigely et al.   BIOCHEMISTRY

Protein engineering of cellobiose dehydrogenase from Phanerochaete chrysosporium in yeast Saccharomyces cerevisiae InvSc1 for increased activity and stability

(2019) Marija Blažić et al.   BIOCHEMICAL ENGINEERING JOURNAL

pM to µM – Elucidating the Role of Distal Mutations in HIV-1 Protease in Conferring Drug Resistance

(2019) Mina Henes et al.   ACS Chemical Biology

Enzyme-Catalyzed Kinetic Resolution of Chiral Precursors to Antiviral Prodrugs

(2019) Dao Feng Xiang et al.   BIOCHEMISTRY

Nanoparticle Size Influences Localized Enzymatic Enhancement—A Case Study with Phosphotriesterase

(2019) Joyce C. Breger et al.   BIOCONJUGATE CHEMISTRY

Advances in detection of hazardous organophosphorus compounds using organophosphorus hydrolase based biosensors

(2019) Monika Jain et al.   CRITICAL REVIEWS IN TOXICOLOGY

Pollution status and bioremediation of chlorpyrifos in environmental matrices by the application of bacterial communities: A review

(2019) Mohd Ashraf Dar et al.   JOURNAL OF ENVIRONMENTAL MANAGEMENT

A Comprehensive Understanding of Enzymatic Degradation of the G-Type Nerve Agent by Phosphotriesterase: Revised Role of Water Molecules and Rate-Limiting Product Release

(2019) Fangfang Fan et al.   ACS Catalysis

The evolution of phosphotriesterase for decontamination and detoxification of organophosphorus chemical warfare agents

(2019) Andrew N. Bigley et al.   CHEMICO-BIOLOGICAL INTERACTIONS

Microbial Phosphotriesterase: Structure, Function, and Biotechnological Applications

(2019) Wahhida Latip et al.   Catalysts

Simple Purification and Immobilization of His-Tagged Organophosphohydrolase from Cell Culture Supernatant by Metal Organic Frameworks for Degradation of Organophosphorus Pesticides

(2019) Saiguang Xue et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

The effect of divalent metal chelators and cadmium on serum phosphotriesterase, lactonase and arylesterase activities of paraoxonase 1

(2018) Anna Bizoń et al.   ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY

Murders with VX: Aum Shinrikyo in Japan and the assassination of Kim Jong-Nam in Malaysia

(2018) Tomomasa Nakagawa et al.   Forensic Toxicology

Enzymatic detoxification of organophosphorus pesticides and related toxicants

(2018) Karla Alejo-González et al.   JOURNAL OF PESTICIDE SCIENCE

Automated Design of Efficient and Functionally Diverse Enzyme Repertoires

(2018) Olga Khersonsky et al.   MOLECULAR CELL

Expressing a Monomeric Organophosphate Hydrolase as an EK Fusion Protein

(2018) Erik J. Liu et al.   BIOCONJUGATE CHEMISTRY

Improvement of the organic solvent stability of a commercial lipase by chemical modification with dextran

(2018) Shota Kajiwara et al.   BIOCHEMICAL ENGINEERING JOURNAL

DNA Nanostructure Sequence-Dependent Binding of Organophosphates

(2017) Yingning Gao et al.   LANGMUIR

Overcoming an optimization plateau in the directed evolution of highly efficient nerve agent bioscavengers

(2017) Moshe Goldsmith et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Diffusion, adsorption and reaction of glucose in glucose oxidase enzyme immobilized mesoporous silica (SBA-15) particles: Experiments and modeling

(2016) Paulami Majumdar et al.   BIOCHEMICAL ENGINEERING JOURNAL

Organophosphate-degrading metallohydrolases: Structure and function of potent catalysts for applications in bioremediation

(2016) Gerhard Schenk et al.   COORDINATION CHEMISTRY REVIEWS

Organophosphate Hydrolase Is a Lipoprotein and Interacts with Pi-specific Transport System to Facilitate Growth ofBrevundimonas diminutaUsing OP Insecticide as Source of Phosphate

(2016) Sunil Parthasarathy et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Encapsulation of a Nerve Agent Detoxifying Enzyme by a Mesoporous Zirconium Metal–Organic Framework Engenders Thermal and Long-Term Stability

(2016) Peng Li et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability

(2016) Adi Goldenzweig et al.   MOLECULAR CELL

The role of protein dynamics in the evolution of new enzyme function

(2016) Eleanor Campbell et al.   Nature Chemical Biology

Recent approaches to improving selectivity and sensitivity of enzyme-based biosensors for organophosphorus pesticides: A review

(2016) Everlyne A. Songa et al.   TALANTA

Understanding How Nanoparticle Attachment Enhances Phosphotriesterase Kinetic Efficiency

(2015) Joyce C. Breger et al.   ACS Nano

Efficient Surface Display of Diisopropylfluorophosphatase (DFPase) in E. coli for Biodegradation of Toxic Organophosphorus Compounds (DFP and Cp)

(2015) Ali Mohammad Latifi et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Catalytic efficiencies of directly evolved phosphotriesterase variants with structurally different organophosphorus compounds in vitro

(2015) Moshe Goldsmith et al.   ARCHIVES OF TOXICOLOGY

Dual-functional OPH-immobilized polyamide nanofibrous membrane for effective organophosphorus toxic agents protection

(2015) Xiao-Yu Yan et al.   BIOCHEMICAL ENGINEERING JOURNAL

Protein engineering of representative hydrolytic enzymes for remediation of organophosphates

(2015) Rupa Iyer et al.   BIOCHEMICAL ENGINEERING JOURNAL

Variants of Phosphotriesterase for the Enhanced Detoxification of the Chemical Warfare Agent VR

(2015) Andrew N. Bigley et al.   BIOCHEMISTRY

EKylation: Addition of an Alternating-Charge Peptide Stabilizes Proteins

(2015) Erik J. Liu et al.   BIOMACROMOLECULES

Quantum dot display enhances activity of a phosphotriesterase trimer

(2015) Joyce C. Breger et al.   CHEMICAL COMMUNICATIONS

Zwitterionic gel encapsulation promotes protein stability, enhances pharmacokinetics, and reduces immunogenicity

(2015) Peng Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Sulfhydryl-specific PEGylation of phosphotriesterase cysteine mutants for organophosphate detoxification

(2015) Gurdip K. Daffu et al.   PROTEIN ENGINEERING DESIGN & SELECTION

PEGylation kinetics of recombinant hirudin and its application for the production of PEGylated HV2 species

(2014) Xudong Wang et al.   BIOCHEMICAL ENGINEERING JOURNAL

Post-exposure treatment of VX poisoned guinea pigs with the engineered phosphotriesterase mutant C23: A proof-of-concept study

(2014) Franz Worek et al.   TOXICOLOGY LETTERS

Engineering V-Type Nerve Agents Detoxifying Enzymes Using Computationally Focused Libraries

(2013) Izhack Cherny et al.   ACS Chemical Biology

Gates of Enzymes

(2013) Artur Gora et al.   CHEMICAL REVIEWS

Molecular Engineering of Organophosphate Hydrolysis Activity from a Weak Promiscuous Lactonase Template

(2013) Monika M. Meier et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Enzymatic Neutralization of the Chemical Warfare Agent VX: Evolution of Phosphotriesterase for Phosphorothiolate Hydrolysis

(2013) Andrew N. Bigley et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Improving methyl parathion hydrolase to enhance its chlorpyrifos-hydrolysing efficiency

(2013) J. Xie et al.   LETTERS IN APPLIED MICROBIOLOGY

Substitution of the Catalytic Metal and Protein PEGylation Enhances Activity and Stability of Bacterial Phosphotriesterase

(2012) Lucia Perezgasga et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Reconstructing a Missing Link in the Evolution of a Recently Diverged Phosphotriesterase by Active-Site Loop Remodeling

(2012) Livnat Afriat-Jurnou et al.   BIOCHEMISTRY

Catalytic mechanisms for phosphotriesterases

(2012) Andrew N. Bigley et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Organophosphorus hydrolase as an in vivo catalytic nerve agent bioscavenger

(2012) Melinda E. Wales et al.   Drug Testing and Analysis

Hydrolysis of organophosphorus pesticides in soil: New opportunities with ecocompatible immobilized His6-OPH

(2012) M. Sirotkina et al.   INTERNATIONAL BIODETERIORATION & BIODEGRADATION

Enhanced Enzymatic Activity of OPH in Ammonium-Functionalized Mesoporous Silica: Surface Modification and Pore Effects

(2012) Kheireddine El-Boubbou et al.   Journal of Physical Chemistry C

Computational redesign of a mononuclear zinc metalloenzyme for organophosphate hydrolysis

(2012) Sagar D Khare et al.   Nature Chemical Biology

Diminishing returns and tradeoffs constrain the laboratory optimization of an enzyme

(2012) Nobuhiko Tokuriki et al.   Nature Communications

Enhanced Enzymatic Thermal Stability and Activity in Functionalized Mesoporous Silica Monitored by 31P NMR

(2012) Kheireddine El-Boubbou et al.   Advanced Healthcare Materials

Destruction and Detection of Chemical Warfare Agents

(2011) Kibong Kim et al.   CHEMICAL REVIEWS

FDA-approved poly(ethylene glycol)–protein conjugate drugs

(2011) Steevens N. S. Alconcel et al.   Polymer Chemistry

The organophosphate-degrading enzyme fromAgrobacterium radiobacterdisplays mechanistic flexibility for catalysis

(2010) Fernanda Ely et al.   BIOCHEMICAL JOURNAL

Stereoselective Hydrolysis of Organophosphate Nerve Agents by the Bacterial Phosphotriesterase

(2010) Ping-Chuan Tsai et al.   BIOCHEMISTRY

Immobilization of organophosphate hydrolase on an amyloid fibril nanoscaffold: Towards bioremediation and chemical detoxification

(2010) Jared K. Raynes et al.   BIOTECHNOLOGY PROGRESS

Acute pesticide poisoning: a proposed classification tool

(2010) Josef Thundiyil   BULLETIN OF THE WORLD HEALTH ORGANIZATION

Improved pharmacokinetics and immunogenicity profile of organophosphorus hydrolase by chemical modification with polyethylene glycol

(2010) Boris N. Novikov et al.   JOURNAL OF CONTROLLED RELEASE

Impact of Distal Mutation on Hydrogen Transfer Interface and Substrate Conformation in Soybean Lipoxygenase

(2010) Sarah J. Edwards et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Structural Insights into the Dual Activities of the Nerve Agent Degrading Organophosphate Anhydrolase/Prolidase

(2009) Nand K. Vyas et al.   BIOCHEMISTRY

Molecular dynamics simulations of the detoxification of paraoxon catalyzed by phosphotriesterase

(2009) Xin Zhang et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

Acute pesticide poisoning among agricultural workers in the United States, 1998-2005

(2008) Geoffrey M. Calvert et al.   AMERICAN JOURNAL OF INDUSTRIAL MEDICINE

Structural Basis for Natural Lactonase and Promiscuous Phosphotriesterase Activities

(2008) Mikael Elias et al.   JOURNAL OF MOLECULAR BIOLOGY

Protein Resistance of PEG-Functionalized Dendronized Surfaces: Effect of PEG Molecular Weight and Dendron Generation

(2008) S. Rahima Benhabbour et al.   MACROMOLECULES

Balancing the stability and the catalytic specificities of OP hydrolases with enhanced V-agent activities

(2008) T.E. Reeves et al.   PROTEIN ENGINEERING DESIGN & SELECTION

In Crystallo Capture of a Michaelis Complex and Product-binding Modes of a Bacterial Phosphotriesterase

(2007) Colin J. Jackson et al.   JOURNAL OF MOLECULAR BIOLOGY