The CRISPR revolution and its potential impact on global health security

Outbreak of coronavirus disease 2019

(2020) Talha Burki   LANCET INFECTIOUS DISEASES

Development of CRISPR as an Antiviral Strategy to Combat SARS-CoV-2 and Influenza

(2020) Timothy R. Abbott et al.   CELL

A Mouse Model of SARS-CoV-2 Infection and Pathogenesis

(2020) Shi-Hui Sun et al.   Cell Host & Microbe

Highly Parallel Profiling of Cas9 Variant Specificity

(2020) Jonathan L. Schmid-Burgk et al.   MOLECULAR CELL

Massively multiplexed nucleic acid detection with Cas13

(2020) Cheri M. Ackerman et al.   NATURE

CRISPR–Cas12-based detection of SARS-CoV-2

(2020) James P. Broughton et al.   NATURE BIOTECHNOLOGY

PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage

(2020) Tautvydas Karvelis et al.   NUCLEIC ACIDS RESEARCH

How CRISPR-Cas System Could Be Used to Combat Antimicrobial Resistance

(2020) Pourya Gholizadeh et al.   Infection and Drug Resistance

Open Sharing During COVID-19: CRISPR-Based Detection Tools

(2020) Jennifer Tsang et al.   CRISPR Journal

Identification of preexisting adaptive immunity to Cas9 proteins in humans

(2019) Carsten T. Charlesworth et al.   NATURE MEDICINE

Adopt a moratorium on heritable genome editing

(2019) Eric S. Lander et al.   NATURE

Worldwide CRISPR patent landscape shows strong geographical biases

(2019) Jacqueline Martin-Laffon et al.   NATURE BIOTECHNOLOGY

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors

(2018) Silvana Konermann et al.   CELL

CRISPR RNAs trigger innate immune responses in human cells

(2018) Sojung Kim et al.   GENOME RESEARCH

CRISPR RNA-Dependent Binding and Cleavage of Endogenous RNAs by the Campylobacter jejuni Cas9

(2018) Gaurav Dugar et al.   MOLECULAR CELL

Base editing with a Cpf1–cytidine deaminase fusion

(2018) Xiaosa Li et al.   NATURE BIOTECHNOLOGY

Efficient and precise editing of endogenous transcripts with SNAP-tagged ADARs

(2018) Paul Vogel et al.   NATURE METHODS

Field-deployable viral diagnostics using CRISPR-Cas13

(2018) Cameron Myhrvold et al.   SCIENCE

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

(2018) Jonathan S. Gootenberg et al.   SCIENCE

Recombinase polymerase amplification: Basics, applications and recent advances

(2018) Ivan Magriñá Lobato et al.   TRAC-TRENDS IN ANALYTICAL CHEMISTRY

RNA-dependent RNA targeting by CRISPR-Cas9

(2018) Steven C Strutt et al.   eLife

The WHO R&D Blueprint: 2018 review of emerging infectious diseases requiring urgent research and development efforts

(2018) Massinissa Si Mehand et al.   ANTIVIRAL RESEARCH

CRISPR-Cas guides the future of genetic engineering

(2018) Gavin J. Knott et al.   SCIENCE

CRISPR-baby scientist fails to satisfy critics

(2018) David Cyranoski   NATURE

CRISPR twins: a condemnation from Chinese academic societies

(2018) Haoyi Wang et al.   NATURE

Organizers of gene-editing meeting blast Chinese study but call for ‘pathway’ to human trials

(2018) Dennis Normile   SCIENCE

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes

(2018) Lucas B. Harrington et al.   SCIENCE

Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a

(2017) Daan C. Swarts et al.   MOLECULAR CELL

Cas13b Is a Type VI-B CRISPR-Associated RNA-Guided RNase Differentially Regulated by Accessory Proteins Csx27 and Csx28

(2017) Aaron A. Smargon et al.   MOLECULAR CELL

Methods and Applications of CRISPR-Mediated Base Editing in Eukaryotic Genomes

(2017) Gaelen T. Hess et al.   MOLECULAR CELL

In Vivo Excision of HIV-1 Provirus by saCas9 and Multiplex Single-Guide RNAs in Animal Models

(2017) Chaoran Yin et al.   MOLECULAR THERAPY

Enhanced proofreading governs CRISPR–Cas9 targeting accuracy

(2017) Janice S. Chen et al.   NATURE

RNA targeting with CRISPR–Cas13

(2017) Omar O. Abudayyeh et al.   NATURE

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

(2017) Nicole M. Gaudelli et al.   NATURE

CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR–Cas9 nuclease off-targets

(2017) Shengdar Q Tsai et al.   NATURE METHODS

Diversity and evolution of class 2 CRISPR–Cas systems

(2017) Sergey Shmakov et al.   NATURE REVIEWS MICROBIOLOGY

Contemporary H3N2 influenza viruses have a glycosylation site that alters binding of antibodies elicited by egg-adapted vaccine strains

(2017) Seth J. Zost et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Antiviral Goes Viral: Harnessing CRISPR/Cas9 to Combat Viruses in Humans

(2017) Jasper Adriaan Soppe et al.   TRENDS IN MICROBIOLOGY

CRISPR-Mediated Integration of Large Gene Cassettes Using AAV Donor Vectors

(2017) Rasmus O. Bak et al.   Cell Reports

Drug resistance in influenza A virus: the epidemiology and management

(2017) Mazhar Hussain et al.   Infection and Drug Resistance

Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity

(2017) Alexis C. Komor et al.   Science Advances

Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo induces homology-directed DNA repair

(2017) Kunwoo Lee et al.   Nature Biomedical Engineering

Non-Homologous End Joining and Homology Directed DNA Repair Frequency of Double-Stranded Breaks Introduced by Genome Editing Reagents

(2017) Michail Zaboikin et al.   PLoS One

Inheritable Silencing of Endogenous Genes by Hit-and-Run Targeted Epigenetic Editing

(2016) Angelo Amabile et al.   CELL

Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components

(2016) Keith Pardee et al.   CELL

CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs

(2016) Mohammad A. Mandegar et al.   Cell Stem Cell

Determining antigen specificity of a monoclonal antibody using genome-scale CRISPR-Cas9 knockout library

(2016) Anastasia Zotova et al.   JOURNAL OF IMMUNOLOGICAL METHODS

An Outbreak of Ebola Virus Disease in the Lassa Fever Zone

(2016) Augustine Goba et al.   JOURNAL OF INFECTIOUS DISEASES

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection

(2016) Alexandra East-Seletsky et al.   NATURE

CRISPR mouse model boom, rat model renaissance

(2016) Eric Smalley   NATURE BIOTECHNOLOGY

Cornerstones of CRISPR–Cas in drug discovery and therapy

(2016) Christof Fellmann et al.   NATURE REVIEWS DRUG DISCOVERY

After Ebola in West Africa — Unpredictable Risks, Preventable Epidemics

(2016)   NEW ENGLAND JOURNAL OF MEDICINE

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

(2016) Omar O. Abudayyeh et al.   SCIENCE

CRISPR/Cas9-Derived Mutations Both Inhibit HIV-1 Replication and Accelerate Viral Escape

(2016) Zhen Wang et al.   Cell Reports

CRISPR/Cas9: Implications for Modeling and Therapy of Neurodegenerative Diseases

(2016) Weili Yang et al.   Frontiers in Molecular Neuroscience

A Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks

(2015) Oren Parnas et al.   CELL

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

A light-inducible CRISPR-Cas9 system for control of endogenous gene activation

(2015) Lauren R Polstein et al.   Nature Chemical Biology

Cas9-mediated targeting of viral RNA in eukaryotic cells

(2015) Aryn A. Price et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Cas9-guide RNA complex preorganized for target DNA recognition

(2015) F. Jiang et al.   SCIENCE

The history and market impact of CRISPR RNA-guided nucleases

(2015) Paul BG van Erp et al.   Current Opinion in Virology

Genome Editing and Its Applications in Model Organisms

(2015) Dongyuan Ma et al.   GENOMICS PROTEOMICS & BIOINFORMATICS

Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases

(2014) Robert J Citorik et al.   NATURE BIOTECHNOLOGY

CRISPR-Cas systems for editing, regulating and targeting genomes

(2014) Jeffry D Sander et al.   NATURE BIOTECHNOLOGY

Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials

(2014) David Bikard et al.   NATURE BIOTECHNOLOGY

Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems

(2014) A. A. Gomaa et al.   mBio

Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System

(2013) Baohui Chen et al.   CELL

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

CRISPR interference (CRISPRi) for sequence-specific control of gene expression

(2013) Matthew H Larson et al.   Nature Protocols

New treatments for influenza

(2012) Sailen Barik   BMC Medicine

Experimental adaptation of an influenza H5 HA confers respiratory droplet transmission to a reassortant H5 HA/H1N1 virus in ferrets

(2012) Masaki Imai et al.   NATURE

A transcription activator-like effector toolbox for genome engineering

(2012) Neville E Sanjana et al.   Nature Protocols

Isolation of a Novel Coronavirus from a Man with Pneumonia in Saudi Arabia

(2012) Ali M. Zaki et al.   NEW ENGLAND JOURNAL OF MEDICINE

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Airborne Transmission of Influenza A/H5N1 Virus Between Ferrets

(2012) S. Herfst et al.   SCIENCE

RNAi screening: new approaches, understandings, and organisms

(2011) Stephanie E. Mohr et al.   Wiley Interdisciplinary Reviews-RNA

Do we need new antibiotics? The search for new targets and new compounds

(2010) Jaroslav Spížek et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Natural Resistance to HIV Infection: Lessons Learned from HIV‐Exposed Uninfected Individuals

(2010) Gianfranco Pancino et al.   JOURNAL OF INFECTIOUS DISEASES

Genome editing with engineered zinc finger nucleases

(2010) Fyodor D. Urnov et al.   NATURE REVIEWS GENETICS

The Public's Response to the 2009 H1N1 Influenza Pandemic

(2010) Gillian K. SteelFisher et al.   NEW ENGLAND JOURNAL OF MEDICINE

Origins and evolutionary genomics of the 2009 swine-origin H1N1 influenza A epidemic

(2009) Gavin J. D. Smith et al.   NATURE