Leveraging the wheat germ cell-free protein synthesis system to accelerate malaria vaccine development

Development and validation of serological markers for detecting recent Plasmodium vivax infection

(2020) Rhea J. Longley et al.   NATURE MEDICINE

Antibodies against a short region of PfRipr inhibit Plasmodium falciparum merozoite invasion and PfRipr interaction with Rh5 and SEMA7A

(2020) Hikaru Nagaoka et al.   Scientific Reports

Global Repertoire of Human Antibodies Against Plasmodium falciparum RIFINs, SURFINs, and STEVORs in a Malaria Exposed Population

(2020) Bernard N. Kanoi et al.   Frontiers in Immunology

Next-generation vaccine platforms for COVID-19

(2020) Debby van Riel et al.   NATURE MATERIALS

Malaria vaccines since 2000: progress, priorities, products

(2020) Patrick E. Duffy et al.   npj Vaccines

Structure and function of a malaria transmission blocking vaccine targeting Pfs230 and Pfs230-Pfs48/45 proteins

(2020) Kavita Singh et al.   Communications Biology

Malaria vaccines in the eradication era: current status and future perspectives

(2019) K Wilson et al.   Expert Review of Vaccines

Current challenges and proposed solutions to the effective implementation of the RTS, S/AS01 Malaria Vaccine Program in sub-Saharan Africa: A systematic review

(2019) Christian Akem Dimala et al.   PLoS One

Exploitation of reverse vaccinology and immunoinformatics as promising platform for genome-wide screening of new effective vaccine candidates against Plasmodium falciparum

(2019) Manisha Pritam et al.   BMC BIOINFORMATICS

PfMSA180 is a novel Plasmodium falciparum vaccine antigen that interacts with human erythrocyte integrin associated protein (CD47)

(2019) Hikaru Nagaoka et al.   Scientific Reports

Artificial intelligence and immunotherapy

(2019) Parnian Jabbari et al.   Expert Review of Clinical Immunology

Sero-diagnostic potential of Plasmodium falciparum recombinant merozoite surface protein (MSP)-3 expressed in silkworm

(2019) Vipin Kumar Deo et al.   PARASITOLOGY INTERNATIONAL

The N-Terminal Region of Plasmodium falciparum MSP10 Is a Target of Protective Antibodies in Malaria and Is Important for PfGAMA/PfMSP10 Interaction

(2019) Hikaru Nagaoka et al.   Frontiers in Immunology

Plasmodium falciparum Blood Stage Antimalarial Vaccines: An Analysis of Ongoing Clinical Trials and New Perspectives Related to Synthetic Vaccines

(2019) David Ricardo Salamanca et al.   Frontiers in Microbiology

Malaria Vaccines: Recent Advances and New Horizons

(2018) Simon J. Draper et al.   Cell Host & Microbe

Interpreting challenge data from early phase malaria blood stage vaccine trials

(2018) Michael F. Good et al.   Expert Review of Vaccines

Unconventional protein secretion – new insights into the pathogenesis and therapeutic targets of human diseases

(2018) Jiyoon Kim et al.   JOURNAL OF CELL SCIENCE

Genetic tools for reliable gene expression and recombineering in Pseudomonas putida

(2018) Taylor B. Cook et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Drug resistance in Plasmodium

(2018) Kasturi Haldar et al.   NATURE REVIEWS MICROBIOLOGY

Identification of target proteins of clinical immunity to Plasmodium falciparum in a region of low malaria transmission

(2018) Hirokazu Sakamoto et al.   PARASITOLOGY INTERNATIONAL

Structure-based design of chimeric antigens for multivalent protein vaccines

(2018) S. Hollingshead et al.   Nature Communications

PV1, a novel Plasmodium falciparum merozoite dense granule protein, interacts with exported protein in infected erythrocytes

(2018) Masayuki Morita et al.   Scientific Reports

Structure-Based Design of Porcine Circovirus Type 2 Chimeric VLPs (cVLPs) Displays Foreign Peptides on the Capsid Surface

(2018) Dongliang Wang et al.   Frontiers in Cellular and Infection Microbiology

Comprehensive analysis of antibody responses to Plasmodium falciparum erythrocyte membrane protein 1 domains

(2018) Bernard N. Kanoi et al.   VACCINE

Author Correction: Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery

(2018) Thapakorn Jaroentomeechai et al.   Nature Communications

Establishing a High-Yielding Cell-Free Protein Synthesis Platform Derived from Vibrio natriegens

(2018) Benjamin J. Des Soye et al.   ACS Synthetic Biology

Enhancing the Biological Relevance of Machine Learning Classifiers for Reverse Vaccinology

(2017) Ashley Heinson et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Immune evasion of Plasmodium falciparum by RIFIN via inhibitory receptors

(2017) Fumiji Saito et al.   NATURE

Antibody profiles to wheat germ cell-free system synthesized Plasmodium falciparum proteins correlate with protection from symptomatic malaria in Uganda

(2017) Bernard N. Kanoi et al.   VACCINE

Naturally acquired antibody responses to more than 300 Plasmodium vivax proteins in three geographic regions

(2017) Rhea J. Longley et al.   PLoS Neglected Tropical Diseases

Characterizing Antibody Responses to Plasmodium vivax and Plasmodium falciparum Antigens in India Using Genome-Scale Protein Microarrays

(2017) Swapna Uplekar et al.   PLoS Neglected Tropical Diseases

Structure of the malaria vaccine candidate antigen CyRPA and its complex with a parasite invasion inhibitory antibody

(2017) Paola Favuzza et al.   eLife

Immunoscreening of Plasmodium falciparum proteins expressed in a wheat germ cell-free system reveals a novel malaria vaccine candidate

(2017) Masayuki Morita et al.   Scientific Reports

Essential Role of the PfRh5/PfRipr/CyRPA Complex during Plasmodium falciparum Invasion of Erythrocytes

(2016) Jennifer C. Volz et al.   Cell Host & Microbe

Progress and prospects for blood-stage malaria vaccines

(2016) Kazutoyo Miura   Expert Review of Vaccines

Reduced immunogenicity ofPlasmodium falciparumgamete surface antigen (Pfs48/45) in mice after disruption of disulphide bonds - evaluating effect of interferon-γ-inducible lysosomal thiol reductase

(2016) Kristen M. Merino et al.   IMMUNOLOGY

Common asymptomatic and submicroscopic malaria infections in Western Thailand revealed in longitudinal molecular and serological studies: a challenge to malaria elimination

(2016) Elisabeth Baum et al.   MALARIA JOURNAL

Development of a Bacillus subtilis cell-free transcription-translation system for prototyping regulatory elements

(2016) Richard Kelwick et al.   METABOLIC ENGINEERING

Functional Antibodies and Protection against Blood-stage Malaria

(2016) Andrew Teo et al.   TRENDS IN PARASITOLOGY

Identification of Plasmodium falciparum reticulocyte binding protein homologue 5-interacting protein, PfRipr, as a highly conserved blood-stage malaria vaccine candidate

(2016) Edward H. Ntege et al.   VACCINE

High-Throughput Sequencing-Based Immune Repertoire Study during Infectious Disease

(2016) Dongni Hou et al.   Frontiers in Immunology

A technique to increase protein yield in a rabbit reticulocyte lysate translation system

(2016) Maria Anastasina et al.   BIOTECHNIQUES

A versatile coupled cell-free transcription-translation system based on tobacco BY-2 cell lysates

(2015) Matthias Buntru et al.   BIOTECHNOLOGY AND BIOENGINEERING

Plant expression and characterization of the transmission-blocking vaccine candidate PfGAP50

(2015) Veronique Beiss et al.   BMC BIOTECHNOLOGY

Efficacy and safety of RTS,S/AS01 malaria vaccine with or without a booster dose in infants and children in Africa: final results of a phase 3, individually randomised, controlled trial

(2015)   LANCET

Alternative Protein Secretion in the Malaria Parasite Plasmodium falciparum

(2015) Thuvaraka Thavayogarajah et al.   PLoS One

Discovery of Novel Plasmodium falciparum Pre-Erythrocytic Antigens for Vaccine Development

(2015) Joao C. Aguiar et al.   PLoS One

Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay

(2015) Hiroyuki Takeda et al.   Scientific Reports

Revealing the Sequence and Resulting Cellular Morphology of Receptor-Ligand Interactions during Plasmodium falciparum Invasion of Erythrocytes

(2015) Greta E. Weiss et al.   PLoS Pathogens

Structural and Computational Biology in the Design of Immunogenic Vaccine Antigens

(2015) Lassi Liljeroos et al.   Journal of Immunology Research

The Baculovirus-Expressed Binding Region of Plasmodium falciparum EBA-140 Ligand and Its Glycophorin C Binding Specificity

(2015) Joanna Rydzak et al.   PLoS One

Structure of the DBL3X-DBL4ε region of the VAR2CSA placental malaria vaccine candidate: insight into DBL domain interactions

(2015) Stéphane Gangnard et al.   Scientific Reports

Synthesis of 2.3 mg/ml of protein with an all Escherichia coli cell-free transcription–translation system

(2014) Filippo Caschera et al.   BIOCHIMIE

A combined cell-free transcription-translation system fromSaccharomyces cerevisiaefor rapid and robust protein synthe

(2014) Rui Gan et al.   Biotechnology Journal

A combined analysis of immunogenicity, antibody kinetics and vaccine efficacy from phase 2 trials of the RTS,S malaria vaccine

(2014) Michael T White et al.   BMC Medicine

Surface antigens of Plasmodium falciparum-infected erythrocytes as immune targets and malaria vaccine candidates

(2014) Jo-Anne Chan et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Predicting Antidisease Immunity Using Proteome Arrays and Sera from Children Naturally Exposed to Malaria

(2014) Olivia C. Finney et al.   MOLECULAR & CELLULAR PROTEOMICS

New antigens for a multicomponent blood-stage malaria vaccine

(2014) F. H. Osier et al.   Science Translational Medicine

Unnatural amino acid incorporation in E. coli: current and future applications in the design of therapeutic proteins

(2014) Kim Wals et al.   Frontiers in Chemistry

Cell-free protein expression based on extracts from CHO cells

(2013) Andreas K. Brödel et al.   BIOTECHNOLOGY AND BIOENGINEERING

Glycans-by-design: Engineering bacteria for the biosynthesis of complex glycans and glycoconjugates

(2013) Judith H. Merritt et al.   BIOTECHNOLOGY AND BIOENGINEERING

Application of wheat germ cell-free protein expression system for novel malaria vaccine candidate discovery

(2013) Thangavelu U Arumugam et al.   Expert Review of Vaccines

Vaccine-induced monoclonal antibodies targeting circumsporozoite protein prevent Plasmodium falciparum infection

(2013) Lander Foquet et al.   JOURNAL OF CLINICAL INVESTIGATION

Identification and Prioritization of Merozoite Antigens as Targets of Protective Human Immunity to Plasmodium falciparum Malaria for Vaccine and Biomarker Development

(2013) J. S. Richards et al.   JOURNAL OF IMMUNOLOGY

Naturally Acquired Antibodies Specific for Plasmodium falciparum Reticulocyte-Binding Protein Homologue 5 Inhibit Parasite Growth and Predict Protection From Malaria

(2013) Tuan M. Tran et al.   JOURNAL OF INFECTIOUS DISEASES

Computational and Experimental Validation of B and T-Cell Epitopes of the In Vivo Immune Response to a Novel Malarial Antigen

(2013) Elke S. Bergmann-Leitner et al.   PLoS One

Asparagine Repeats in Plasmodium falciparum Proteins: Good for Nothing?

(2013) Vasant Muralidharan et al.   PLoS Pathogens

The cellular and molecular basis for malaria parasite invasion of the human red blood cell

(2012) Alan F. Cowman et al.   JOURNAL OF CELL BIOLOGY

Algae-Produced Pfs25 Elicits Antibodies That Inhibit Malaria Transmission

(2012) James A. Gregory et al.   PLoS One

Cell-free protein synthesis: Applications come of age

(2011) Erik D. Carlson et al.   BIOTECHNOLOGY ADVANCES

Discovery of GAMA, a Plasmodium falciparum Merozoite Micronemal Protein, as a Novel Blood-Stage Vaccine Candidate Antigen

(2011) Thangavelu U. Arumugam et al.   INFECTION AND IMMUNITY

Plasmodium vivax: comparison of immunogenicity among proteins expressed in the cell-free systems of Escherichia coli and wheat germ by suspension array assays

(2011) Edmilson Rui et al.   MALARIA JOURNAL

Binding ofPlasmodiummerozoite proteins RON2 and AMA1 triggers commitment to invasion

(2011) Prakash Srinivasan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Research Agenda to Underpin Malaria Eradication

(2011) Pedro L. Alonso et al.   PLOS MEDICINE

Simple Screening Method for Autoantigen Proteins Using the N-Terminal Biotinylated Protein Library Produced by Wheat Cell-Free Synthesis

(2010) Kazuhiro Matsuoka et al.   JOURNAL OF PROTEOME RESEARCH

Practical cell-free protein synthesis system using purified wheat embryos

(2010) Kazuyuki Takai et al.   Nature Protocols

A prospective analysis of the Ab response to Plasmodium falciparum before and after a malaria season by protein microarray

(2010) P. D. Crompton et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Production of recombinant proteins from protozoan parasites

(2010) José A. Fernández-Robledo et al.   TRENDS IN PARASITOLOGY

Signal initiation in biological systems: the properties and detection of transient extracellular protein interactions

(2009) Gavin J. Wright   Molecular BioSystems

Species-independent translational leaders facilitate cell-free expression

(2009) Sergei Mureev et al.   NATURE BIOTECHNOLOGY

Plasmodium berghei HAP2 induces strong malaria transmission-blocking immunity in vivo and in vitro

(2009) Andrew M. Blagborough et al.   VACCINE

Wheat Germ Cell-Free System-Based Production of Malaria Proteins for Discovery of Novel Vaccine Candidates

(2008) T. Tsuboi et al.   INFECTION AND IMMUNITY

Morphology and kinetics of the three distinct phases of red blood cell invasion by Plasmodium falciparum merozoites

(2008) Paul R. Gilson et al.   INTERNATIONAL JOURNAL FOR PARASITOLOGY

Heterologous expression of plasmodial proteins for structural studies and functional annotation

(2008) Lyn-Marie Birkholtz et al.   MALARIA JOURNAL

Cohort study of the association of antibody levels to AMA1, MSP119, MSP3 and GLURP with protection from clinical malaria in Ghanaian children

(2008) Daniel Dodoo et al.   MALARIA JOURNAL

Profiling humoral immune responses to P. falciparum infection with protein microarrays

(2008) Denise L. Doolan et al.   PROTEOMICS