Protein A does not induce allosteric structural changes in an IgG1 antibody during binding

Mass Spectrometry Characterization of Higher Order Structural Changes Associated with the Fc-glycan Structure of the NISTmAb Reference Material, RM 8761

(2020) Kate Groves et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Antigen-Induced Allosteric Changes in a Human IgG1 Fc Increase Low-Affinity Fcγ Receptor Binding

(2020) Chiara Orlandi et al.   STRUCTURE

Identification of IgG1 Aggregation Initiation Region by Hydrogen/Deuterium Mass Spectrometry

(2019) Masanori Noda et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Future perspectives of therapeutic monoclonal antibodies

(2019) Kanta Tsumoto et al.   Immunotherapy

Reliable identification of significant differences in differential HX-MS measurements using a hybrid significance testing approach

(2019) Tyler S Hageman et al.   ANALYTICAL CHEMISTRY

Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments

(2019) Glenn R. Masson et al.   NATURE METHODS

Long-Range Conformational Changes in Monoclonal Antibodies Revealed Using FPOP-LC-MS/MS

(2019) Owen Cornwell et al.   ANALYTICAL CHEMISTRY

The NISTmAb Reference Material 8671 lifecycle management and quality plan

(2018) John E. Schiel et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Analytical comparability study of recombinant monoclonal antibody therapeutics

(2018) Alexandre Ambrogelly et al.   mAbs

The pearl necklace model in Protein A chromatography - molecular mechanisms at the resin interface

(2018) Goncalo L. Silva et al.   BIOTECHNOLOGY AND BIOENGINEERING

Protein aggregation – Mechanisms, detection, and control

(2018) Wei Wang et al.   INTERNATIONAL JOURNAL OF PHARMACEUTICS

Antigen binding allosterically promotes Fc receptor recognition

(2018) Jun Zhao et al.   mAbs

Antibodies to watch in 2019

(2018) Hélène Kaplon et al.   mAbs

IgG cooperativity – Is there allostery? Implications for antibody functions and therapeutic antibody development

(2017) Danlin Yang et al.   mAbs

3-2-1: Structural insights from stepwise shrinkage of a three-helix Fc-binding domain to a single helix

(2017) M Ultsch et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Differential binding of heavy chain variable domain 3 antigen binding fragments to protein a chromatography resins

(2015) Julia Bach et al.   JOURNAL OF CHROMATOGRAPHY A

Transient conformational modification of immunoglobulin G during purification by protein A affinity chromatography

(2015) Pete Gagnon et al.   JOURNAL OF CHROMATOGRAPHY A

Suppression of conformational heterogeneity at a protein–protein interface

(2015) Lindsay N. Deis et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Conformation and Dynamics of Interchain Cysteine-Linked Antibody-Drug Conjugates as Revealed by Hydrogen/Deuterium Exchange Mass Spectrometry

(2014) Lucy Yan Pan et al.   ANALYTICAL CHEMISTRY

Understanding the Conformational Impact of Chemical Modifications on Monoclonal Antibodies with Diverse Sequence Variation Using Hydrogen/Deuterium Exchange Mass Spectrometry and Structural Modeling

(2014) Aming Zhang et al.   ANALYTICAL CHEMISTRY

Engineering of novel Staphylococcal Protein A ligands to enable milder elution pH and high dynamic binding capacity

(2014) Timothy M. Pabst et al.   JOURNAL OF CHROMATOGRAPHY A

Correlations between changes in conformational dynamics and physical stability in a mutant IgG1 mAb engineered for extended serum half-life

(2014) Ranajoy Majumdar et al.   mAbs

Structure of a human IgA1 Fab fragment at 1.55 Å resolution: potential effect of the constant domains on antigen-affinity modulation

(2013) Agustin Correa et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Effects of Salts from the Hofmeister Series on the Conformational Stability, Aggregation Propensity, and Local Flexibility of an IgG1 Monoclonal Antibody

(2013) Ranajoy Majumdar et al.   BIOCHEMISTRY

Correlating Excipient Effects on Conformational and Storage Stability of an IgG1 Monoclonal Antibody with Local Dynamics as Measured by Hydrogen/Deuterium-Exchange Mass Spectrometry

(2013) Prakash Manikwar et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

The constant region affects antigen binding of antibodies to DNA by altering secondary structure

(2013) Yumin Xia et al.   MOLECULAR IMMUNOLOGY

Minimizing Carry-Over in an Online Pepsin Digestion System used for the H/D Exchange Mass Spectrometric Analysis of an IgG1 Monoclonal Antibody

(2012) Ranajoy Majumdar et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Protein G, Protein A and Protein A-Derived Peptides Inhibit the Agitation Induced Aggregation of IgG

(2012) Jun Zhang et al.   MOLECULAR PHARMACEUTICS

Recovery and purification process development for monoclonal antibody production

(2010) Hui F. Liu et al.   mAbs

Post-translational Modifications Differentially Affect IgG1 Conformation and Receptor Binding

(2010) Damian Houde et al.   MOLECULAR & CELLULAR PROTEOMICS

Characterization of IgG1 Conformation and Conformational Dynamics by Hydrogen/Deuterium Exchange Mass Spectrometry

(2009) Damian Houde et al.   ANALYTICAL CHEMISTRY