Correlations between changes in conformational dynamics and physical stability in a mutant IgG1 mAb engineered for extended serum half-life

Hydrogen-Deuterium Exchange Mass Spectrometry as an Emerging Analytical Tool for Stabilization and Formulation Development of Therapeutic Monoclonal Antibodies

(2015) Ranajoy Majumdar et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Conformation and Dynamics of Interchain Cysteine-Linked Antibody-Drug Conjugates as Revealed by Hydrogen/Deuterium Exchange Mass Spectrometry

(2014) Lucy Yan Pan et al.   ANALYTICAL CHEMISTRY

Understanding the Conformational Impact of Chemical Modifications on Monoclonal Antibodies with Diverse Sequence Variation Using Hydrogen/Deuterium Exchange Mass Spectrometry and Structural Modeling

(2014) Aming Zhang et al.   ANALYTICAL CHEMISTRY

Transient Antibody-Mucin Interactions Produce a Dynamic Molecular Shield against Viral Invasion

(2014) Alex Chen et al.   BIOPHYSICAL JOURNAL

Structural Insights into Neonatal Fc Receptor-based Recycling Mechanisms

(2014) Vaheh Oganesyan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Physical Stability Comparisons of IgG1-Fc Variants: Effects of N-Glycosylation Site Occupancy and Asp/Gln Residues at Site Asn 297

(2014) Mohammad A. Alsenaidy et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Combined glyco- and protein-Fc engineering simultaneously enhance cytotoxicity and half-life of a therapeutic antibody

(2014) Céline Monnet et al.   mAbs

A Novel Investigational Fc-Modified Humanized Monoclonal Antibody, Motavizumab-YTE, Has an Extended Half-Life in Healthy Adults

(2013) Gabriel J. Robbie et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Effects of Salts from the Hofmeister Series on the Conformational Stability, Aggregation Propensity, and Local Flexibility of an IgG1 Monoclonal Antibody

(2013) Ranajoy Majumdar et al.   BIOCHEMISTRY

Engineered Soluble Monomeric IgG1 CH3 Domain

(2013) Tianlei Ying et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

High-Throughput Biophysical Analysis and Data Visualization of Conformational Stability of an IgG1 Monoclonal Antibody After Deglycosylation

(2013) Mohammad A. Alsenaidy et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Investigating Monoclonal Antibody Aggregation Using a Combination of H/DX-MS and Other Biophysical Measurements

(2013) Roxana E. Iacob et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Correlating Excipient Effects on Conformational and Storage Stability of an IgG1 Monoclonal Antibody with Local Dynamics as Measured by Hydrogen/Deuterium-Exchange Mass Spectrometry

(2013) Prakash Manikwar et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Understanding the Relationship Between Biotherapeutic Protein Stability and Solid–Liquid Interfacial Shear in Constant Region Mutants of IgG1 and IgG4

(2013) Roumteen Tavakoli-Keshe et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Mutation of Y407 in the CH3 domain dramatically alters glycosylation and structure of human IgG

(2013) Rebecca J. Rose et al.   mAbs

Probing antibody internal dynamics with fluorescence anisotropy and molecular dynamics simulations

(2013) Ekaterine Kortkhonjia et al.   mAbs

Conformational characterization of the charge variants of a human IgG1 monoclonal antibody using H/D exchange mass spectrometry

(2013) Liangjie Tang et al.   mAbs

The interactions of therapeutic antibodies with Fc receptors

(2012) Marcello Albanesi et al.   IMMUNOLOGY LETTERS

Minimizing Carry-Over in an Online Pepsin Digestion System used for the H/D Exchange Mass Spectrometric Analysis of an IgG1 Monoclonal Antibody

(2012) Ranajoy Majumdar et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Monoclonal Antibodies with Identical Fc Sequences Can Bind to FcRn Differentially with Pharmacokinetic Consequences

(2011) W. Wang et al.   DRUG METABOLISM AND DISPOSITION

Forced Degradation of Therapeutic Proteins

(2011) Andrea Hawe et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Neonatal Fc receptor and IgG-based therapeutics

(2011) Timothy T. Kuo et al.   mAbs

Engineering the variable region of therapeutic IgG antibodies

(2011) Tomoyuki Igawa et al.   mAbs

Advances in the assessment and control of the effector functions of therapeutic antibodies

(2011) Xu-Rong Jiang et al.   NATURE REVIEWS DRUG DISCOVERY

Distinct Aggregation Mechanisms of Monoclonal Antibody Under Thermal and Freeze-Thaw Stresses Revealed by Hydrogen Exchange

(2011) Aming Zhang et al.   PHARMACEUTICAL RESEARCH

Glycosylation influences on the aggregation propensity of therapeutic monoclonal antibodies

(2010) Veysel Kayser et al.   Biotechnology Journal

Characterization of protein therapeutics by mass spectrometry: recent developments and future directions

(2010) Guodong Chen et al.   DRUG DISCOVERY TODAY

Importance of Neonatal FcR in Regulating the Serum Half-Life of Therapeutic Proteins Containing the Fc Domain of Human IgG1: A Comparative Study of the Affinity of Monoclonal Antibodies and Fc-Fusion Proteins to Human Neonatal FcR

(2010) Takuo Suzuki et al.   JOURNAL OF IMMUNOLOGY

Charge variants in IgG1

(2010) Leslie A. Khawli et al.   mAbs

Post-translational Modifications Differentially Affect IgG1 Conformation and Receptor Binding

(2010) Damian Houde et al.   MOLECULAR & CELLULAR PROTEOMICS

Development trends for human monoclonal antibody therapeutics

(2010) Aaron L. Nelson et al.   NATURE REVIEWS DRUG DISCOVERY

Conformational changes in oxidatively stressed monoclonal antibodies studied by hydrogen exchange mass spectrometry

(2010) William Burkitt et al.   PROTEIN SCIENCE

Characterization of IgG1 Conformation and Conformational Dynamics by Hydrogen/Deuterium Exchange Mass Spectrometry

(2009) Damian Houde et al.   ANALYTICAL CHEMISTRY

Optimization of Fc-mediated effector functions of monoclonal antibodies

(2009) William R Strohl   CURRENT OPINION IN BIOTECHNOLOGY

Engineered Human Antibody Constant Domains with Increased Stability

(2009) Rui Gong et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural characterization of a human Fc fragment engineered for extended serum half-life

(2009) Vaheh Oganesyan et al.   MOLECULAR IMMUNOLOGY

The Complex Inter-Relationships Between Protein Flexibility and Stability

(2008) Tim J. Kamerzell et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Contribution of Variable Domains to the Stability of Humanized IgG1 Monoclonal Antibodies

(2007) Roxana M. Ionescu et al.   JOURNAL OF PHARMACEUTICAL SCIENCES