A Review: Molecular Chaperone-mediated Folding, Unfolding and Disaggregation of Expressed Recombinant Proteins

CLPB (caseinolytic peptidase B homolog), the first mitochondrial protein refoldase associated with human disease

(2020) Dagmara Mróz et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Dissociation between the critical role of ClpB of Francisella tularensis for the heat shock response and the DnaK interaction and its important role for efficient type VI secretion and bacterial virulence

(2020) Athar Alam et al.   PLoS Pathogens

High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

(2019) Dandan Li et al.   Microbial Cell Factories

Effect of Molecular Chaperone on the Soluble Expression of Recombinant Fab Fragment in E. coli

(2019) Safar Farajnia et al.   International Journal of Peptide Research and Therapeutics

Structural insights into the complex of trigger factor chaperone and ribosomal protein S7 from Mycobacterium tuberculosis

(2019) Zhengyang Li et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Production of active manganese peroxidase in Escherichia coli by co-expression of chaperones and in vitro maturation by ATP-dependent chaperone release

(2019) Almasul Alfi et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Expression of glyceraldehyde-3-phosphate dehydrogenase from M. tuberculosis in E. coli. Purification and characteristics of the untagged recombinant enzyme

(2019) E.V. Schmalhausen et al.   PROTEIN EXPRESSION AND PURIFICATION

Molecular cloning, expression, and functional characterization of 70-kDa heat shock protein, DnaK, from Bacillus halodurans

(2019) Fatemeh Vahdani et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Improvement of soluble expression of GM-CSF in the cytoplasm of Escherichia coli using chemical and molecular chaperones

(2019) Raziyeh Malekian et al.   PROTEIN EXPRESSION AND PURIFICATION

Soluble FMDV VP1 proteins fused with calreticulin expressed in Escherichia coli under the assist of trigger factor16 (Tf16) formed into high immunogenic polymers

(2019) Chang Liu et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Enhanced 5-Aminolevulinic Acid Production by Co-expression of Codon-Optimized hemA Gene with Chaperone in Genetic Engineered Escherichia coli

(2019) Tzu-Hsuan Yu et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Escherichia coli DnaK Allosterically Modulates ClpB between High- and Low-Peptide Affinity States

(2018) Clarissa L. Durie et al.   BIOCHEMISTRY

Bioproduction of trans-10,cis-12-Conjugated Linoleic Acid by a Highly Soluble and Conveniently Extracted Linoleic Acid Isomerase and an Extracellularly Expressed Lipase from Recombinant Escherichia coli Strains

(2018) Meng nan Huang et al.   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Intra-molecular pathways of allosteric control in Hsp70s

(2018) Matthias P. Mayer   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Heterologous expression of Homo sapiens alpha-folate receptors in E. coli by fusion with a trigger factor for enhanced solubilization

(2018) Beatriz Nogueira Messias Miranda et al.   PROTEIN EXPRESSION AND PURIFICATION

Trigger factor assisted self-assembly of canine parvovirus VP2 protein into virus-like particles in Escherichia coli with high immunogenicity

(2018) Liangliang Nan et al.   Virology Journal

Interaction of substrate-mimicking peptides with the AAA+ ATPase ClpB from Escherichia coli

(2018) Chathurange B. Ranaweera et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Structural insight into proline cis/trans isomerization of unfolded proteins catalyzed by the trigger factor chaperone

(2018) Soichiro Kawagoe et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

GroEL/ES mediated the in vivo recovery of TRAIL inclusion bodies in Escherichia coli

(2018) Zhanqing Wang et al.   Scientific Reports

An overview on molecular chaperones enhancing solubility of expressed recombinant proteins with correct folding

(2017) Mina Mamipour et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Effect of DnaK/DnaJ/GrpE and DsbC Chaperons on Periplasmic Expression of Fab Antibody by E. coli SEC Pathway

(2017) Hassan Dariushnejad et al.   International Journal of Peptide Research and Therapeutics

Molecular Cloning, Structural Modeling and the Production of Soluble Triple-Mutated Diphtheria Toxoid (K51E/G52E/E148K) Co-expressed with Molecular Chaperones in Recombinant Escherichia coli

(2017) Naphatsamon Uthailak et al.   MOLECULAR BIOTECHNOLOGY

Introducing site-specific cysteines into nanobodies for mercury labelling allows de novo phasing of their crystal structures

(2017) Simon Boje Hansen et al.   Acta Crystallographica Section D-Structural Biology

Fusion protein analysis reveals the precise regulation between Hsp70 and Hsp100 during protein disaggregation

(2017) Sayaka Hayashi et al.   Scientific Reports

Characterization and Soluble Expression of d-Hydantoinase from Pseudomonas fluorescens for the Synthesis of d-Amino Acids

(2016) Guo-Chao Xu et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

High level accumulation of soluble diphtheria toxin mutant (CRM197) with co-expression of chaperones in recombinant Escherichia coli

(2016) Pornpimol Mahamad et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Deletion of the N- or C-Terminal Helix of Apolipophorin III To Create a Four-Helix Bundle Protein

(2016) Pankaj Dwivedi et al.   BIOCHEMISTRY

Overview of fusion tags for recombinant proteins

(2016) E. N. Kosobokova et al.   BIOCHEMISTRY-MOSCOW

Trigger factor assisted soluble expression of recombinant spike protein of porcine epidemic diarrhea virus in Escherichia coli

(2016) Da-Chuan Piao et al.   BMC BIOTECHNOLOGY

Identification of novelin vivoobligate GroEL/ES substrates based on data from a cell-free proteomics approach

(2016) Tatsuya Niwa et al.   FEBS LETTERS

Chaperonin-enhanced Escherichia coli cell-free expression of functional CXCR4

(2016) Haixia Chi et al.   JOURNAL OF BIOTECHNOLOGY

Enhancement of soluble expression of codon-optimized Thermomicrobium roseum sarcosine oxidase in Escherichia coli via chaperone co-expression

(2016) Yanjun Tong et al.   JOURNAL OF BIOTECHNOLOGY

Expression, purification, and characterization of recombinant human H-chain ferritin

(2016) Wenyan Zou et al.   PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY

Expression, purification, and characterization of recombinant human L-chain ferritin

(2016) Wenyan Zou et al.   PROTEIN EXPRESSION AND PURIFICATION

Enhanced expression of soluble human papillomavirus L1 through coexpression of molecular chaperonin in Escherichia coli

(2016) Dong Pan et al.   PROTEIN EXPRESSION AND PURIFICATION

High-level expression and purification of the major house dust mite allergen Der p 2 in Escherichia coli

(2016) Tuo Cao et al.   PROTEIN EXPRESSION AND PURIFICATION

Improved 1, 2, 4-butanetriol production from an engineered Escherichia coli by co-expression of different chaperone proteins

(2016) Xinyao Lu et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Improving recombinant protein solubility in Escherichia coli: Identification of best chaperone combination which assists folding of human basic fibroblast growth factor

(2016) Alibol Mona et al.   AFRICAN JOURNAL OF BIOTECHNOLOGY

Escherichia coliClpB is a non-processive polypeptide translocase

(2015) Tao Li et al.   BIOCHEMICAL JOURNAL

Protein Folding and Mechanisms of Proteostasis

(2015) José Díaz-Villanueva et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Modulation of the Chaperone DnaK Allosterism by the Nucleotide Exchange Factor GrpE

(2015) Roberto Melero et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A Functional DnaK Dimer Is Essential for the Efficient Interaction with Hsp40 Heat Shock Protein

(2015) Evans Boateng Sarbeng et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Chaperones-assisted soluble expression and maturation of recombinant Co-type nitrile hydratase in Escherichia coli to avoid the need for a low induction temperature

(2015) Xiaolin Pei et al.   JOURNAL OF BIOTECHNOLOGY

Nucleotides regulate the mechanical hierarchy between subdomains of the nucleotide binding domain of the Hsp70 chaperone DnaK

(2015) Daniela Bauer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

ClpB N-terminal domain plays a regulatory role in protein disaggregation

(2015) Rina Rosenzweig et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Expression and in vitro functional analyses of recombinant Gam1 protein

(2015) Gustavo A. Avila et al.   PROTEIN EXPRESSION AND PURIFICATION

High-level soluble expression of a bacterial N-acyl-d-glucosamine 2-epimerase in recombinant Escherichia coli

(2015) Ludwig Klermund et al.   PROTEIN EXPRESSION AND PURIFICATION

Tandem SUMO fusion vectors for improving soluble protein expression and purification

(2015) Fernando Guerrero et al.   PROTEIN EXPRESSION AND PURIFICATION

ClpB chaperone passively threads soluble denatured proteins through its central pore

(2014) Yosuke Nakazaki et al.   GENES TO CELLS

Crystal Structure of a Symmetric Football-Shaped GroEL:GroES2-ATP14 Complex Determined at 3.8 Å Reveals Rearrangement between Two GroEL Rings

(2014) Ayumi Koike-Takeshita et al.   JOURNAL OF MOLECULAR BIOLOGY

Examination of polypeptide substrate specificity forEscherichia coliClpB

(2014) Tao Li et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Structural Basis for Protein Antiaggregation Activity of the Trigger Factor Chaperone

(2014) T. Saio et al.   SCIENCE

Head-to-tail interactions of the coiled-coil domains regulate ClpB activity and cooperation with Hsp70 in protein disaggregation

(2014) Marta Carroni et al.   eLife

Recombinant protein expression in Escherichia coli: advances and challenges

(2014) Germán L. Rosano et al.   Frontiers in Microbiology

Molecular Chaperone Functions in Protein Folding and Proteostasis

(2013) Yujin E. Kim et al.   Annual Review of Biochemistry

Flexibility of the Bacterial Chaperone Trigger Factor in Microsecond-Timescale Molecular Dynamics Simulations

(2013) Andrew S. Thomas et al.   BIOPHYSICAL JOURNAL

Molecular chaperones as enzymes that catalytically unfold misfolded polypeptides

(2013) Smriti Priya et al.   FEBS LETTERS

Diversity in the origins of proteostasis networks — a driver for protein function in evolution

(2013) Evan T. Powers et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Protein rescue from aggregates by powerful molecular chaperone machines

(2013) Shannon M. Doyle et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Heat shock protein (Hsp) 70 is an activator of the Hsp104 motor

(2013) J. Lee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Strategies for the Production of Recombinant Protein in Escherichia coli

(2013) Gopal Jee Gopal et al.   PROTEIN JOURNAL

Unraveling the Mechanism of Protein Disaggregation Through a ClpB-DnaK Interaction

(2013) R. Rosenzweig et al.   SCIENCE

Orientation of the amino-terminal domain of ClpB affects the disaggregation of the protein

(2012) Sayaka Mizuno et al.   FEBS Journal

Crystal Structure of DnaK Protein Complexed with Nucleotide Exchange Factor GrpE in DnaK Chaperone System

(2012) Ching-Chung Wu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Multiple Post-translational Modifications Affect Heterologous Protein Synthesis

(2012) Alexander A. Tokmakov et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure and Allostery of the Chaperonin GroEL

(2012) Helen R. Saibil et al.   JOURNAL OF MOLECULAR BIOLOGY

Structure and Dynamics of the ATP-Bound Open Conformation of Hsp70 Chaperones

(2012) Roman Kityk et al.   MOLECULAR CELL

Hsp70 proteins bind Hsp100 regulatory M domains to activate AAA+ disaggregase at aggregate surfaces

(2012) Fabian Seyffer et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

A tightly regulated molecular toggle controls AAA+ disaggregase

(2012) Yuki Oguchi et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

DnaK Functions as a Central Hub in the E. coli Chaperone Network

(2012) Giulia Calloni et al.   Cell Reports

Mechanics of Hsp70 chaperones enables differential interaction with client proteins

(2011) Rainer Schlecht et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Allosteric signal transmission in the nucleotide-binding domain of 70-kDa heat shock protein (Hsp70) molecular chaperones

(2011) A. Zhuravleva et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mechanisms of the Hsp70 chaperone systemThis paper is one of a selection of papers published in this special issue entitled “Canadian Society of Biochemistry, Molecular & Cellular Biology 52nd Annual Meeting — Protein Folding: Principles and Diseases” and has undergone the Journal's usual peer review process.

(2010) Jason C. Young   Biochemistry and Cell Biology

Structure and function of the molecular chaperone Trigger Factor

(2010) Anja Hoffmann et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

A systematic survey of in vivo obligate chaperonin-dependent substrates

(2010) Kei Fujiwara et al.   EMBO JOURNAL

The HSP70 chaperone machinery: J proteins as drivers of functional specificity

(2010) Harm H. Kampinga et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Production of Recombinant Proteins in the lon-Deficient BL21(DE3) Strain of Escherichia coli in the Absence of the DnaK Chaperone

(2009) J. Ratelade et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Promiscuous Substrate Recognition in Folding and Assembly Activities of the Trigger Factor Chaperone

(2009) Erik Martinez-Hackert et al.   CELL

Converging concepts of protein folding in vitro and in vivo

(2009) F Ulrich Hartl et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Solution conformation of wild-type E. coli Hsp70 (DnaK) chaperone complexed with ADP and substrate

(2009) E. B. Bertelsen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural and Functional Diversity Among Eukaryotic Hsp70 Nucleotide Exchange Factors

(2009) Mehdi Kabani   PROTEIN AND PEPTIDE LETTERS

From hatching to dispatching: the multiple cellular roles of the Hsp70 molecular chaperone machinery

(2008) Eirini Meimaridou et al.   JOURNAL OF MOLECULAR ENDOCRINOLOGY

A peptide deformylase–ribosome complex reveals mechanism of nascent chain processing

(2008) Rouven Bingel-Erlenmeyer et al.   NATURE