Development of a pVEC peptide-based ribonucleoprotein (RNP) delivery system for genome editing using CRISPR/Cas9 in Chlamydomonas reinhardtii

Efficient Editing of the Nuclear APT Reporter Gene in Chlamydomonas reinhardtii via Expression of a CRISPR-Cas9 Module

(2019) Daniel Guzmán-Zapata et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells

(2018) Akiko Seki et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Directed evolution of CRISPR-Cas9 to increase its specificity

(2018) Jungjoon K. Lee et al.   Nature Communications

Optimization of CRISPR/Cas9 Delivery to Human Hematopoietic Stem and Progenitor Cells for Therapeutic Genomic Rearrangements

(2018) Annalisa Lattanzi et al.   MOLECULAR THERAPY

Increasing on-target cleavage efficiency for CRISPR/Cas9-induced large fragment deletion in Myxococcus xanthus

(2017) Ying-jie Yang et al.   Microbial Cell Factories

Lipid production in Nannochloropsis gaditana is doubled by decreasing expression of a single transcriptional regulator

(2017) Imad Ajjawi et al.   NATURE BIOTECHNOLOGY

Targeting of Photoreceptor Genes inChlamydomonas reinhardtiivia Zinc-Finger Nucleases and CRISPR/Cas9

(2017) Andre Greiner et al.   PLANT CELL

Efficient targeted DNA editing and replacement inChlamydomonas reinhardtiiusing Cpf1 ribonucleoproteins and single-stranded DNA

(2017) Aron Ferenczi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Peptide/Cas9 nanostructures for ribonucleoprotein cell membrane transport and gene edition

(2017) Irene Lostalé-Seijo et al.   Chemical Science

CRISPR/Cpf1-mediated DNA-free plant genome editing

(2017) Hyeran Kim et al.   Nature Communications

RNA-Independent DNA Cleavage Activities of Cas9 and Cas12a

(2017) Ramya Sundaresan et al.   Cell Reports

A gene-within-a-gene Cas9/sgRNA hybrid construct enables gene editing and gene replacement strategies in Chlamydomonas reinhardtii

(2017) Wen Zhi Jiang et al.   Algal Research-Biomass Biofuels and Bioproducts

A highly efficient cell penetrating peptide pVEC-mediated protein delivery system into microalgae

(2017) Seongsu Kang et al.   Algal Research-Biomass Biofuels and Bioproducts

Genome editing of model oleaginous microalgaeNannochloropsisspp. by CRISPR/Cas9

(2016) Qintao Wang et al.   PLANT JOURNAL

DNA-free two-gene knockout in Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins

(2016) Kwangryul Baek et al.   Scientific Reports

CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii

(2016) Sung-Eun Shin et al.   Scientific Reports

Heritable CRISPR/Cas9-mediated targeted integration in Xenopus tropicalis

(2015) Zhaoying Shi et al.   FASEB JOURNAL

CRISPR, the disruptor

(2015) Heidi Ledford   NATURE

Off-target Effects in CRISPR/Cas9-mediated Genome Engineering

(2015) Xiao-Hui Zhang et al.   Molecular Therapy-Nucleic Acids

Precise and efficient genome editing in zebrafish using the CRISPR/Cas9 system

(2014) U. Irion et al.   DEVELOPMENT

Successful Transient Expression of Cas9 and Single Guide RNA Genes in Chlamydomonas reinhardtii

(2014) Wenzhi Jiang et al.   EUKARYOTIC CELL

Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA

(2014) S. Ramakrishna et al.   GENOME RESEARCH

Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo

(2014) John A Zuris et al.   NATURE BIOTECHNOLOGY

Implementation of the CRISPR-Cas9 system in fission yeast

(2014) Jake Z. Jacobs et al.   Nature Communications

Direct uptake and degradation of DNA by lysosomes

(2013) Yuuki Fujiwara et al.   Autophagy

CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity

(2013) Thomas J. Cradick et al.   NUCLEIC ACIDS RESEARCH

The Uptake Mechanism of the Cell-Penetrating pVEC Peptide

(2013) Ihsan Omur Akdag et al.   Journal of Chemistry

A molecular method for the delivery of small molecules and proteins across the cell wall of algae using molecular transporters

(2012) J. M. Hyman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

DNA repair in organelles: Pathways, organization, regulation, relevance in disease and aging

(2010) Pierre Boesch et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

FKBP12.6-knockout mice display hyperinsulinemia and resistance to high-fat diet-induced hyperglycemia

(2009) Zheng Chen et al.   FASEB JOURNAL

Comparison of Zinc Finger Nucleases for Use in Gene Targeting in Mammalian Cells

(2008) Shondra M Pruett-Miller et al.   MOLECULAR THERAPY