Increasing on-target cleavage efficiency for CRISPR/Cas9-induced large fragment deletion in Myxococcus xanthus

A highly efficient single-step, markerless strategy for multi-copy chromosomal integration of large biochemical pathways in Saccharomyces cerevisiae

(2016) Shuobo Shi et al.   METABOLIC ENGINEERING

Genome editing in butterflies reveals that spalt promotes and Distal-less represses eyespot colour patterns

(2016) Linlin Zhang et al.   Nature Communications

Internal guide RNA interactions interfere with Cas9-mediated cleavage

(2016) Summer B. Thyme et al.   Nature Communications

Genetic engineering and heterologous expression of the disorazol biosynthetic gene cluster via Red/ET recombineering

(2016) Qiang Tu et al.   Scientific Reports

The complete genome sequence and analysis of a plasmid-bearing myxobacterial strain Myxococcus fulvus 124B02 (M 206081)

(2016) Xiao-jing Chen et al.   Standards in Genomic Sciences

Bacterial Genome Editing with CRISPR-Cas9: Deletion, Integration, Single Nucleotide Modification, and Desirable “Clean” Mutant Selection in Clostridium beijerinckii as an Example

(2016) Yi Wang et al.   ACS Synthetic Biology

CRISPR/Cas9 Based Genome Editing of Penicillium chrysogenum

(2016) C. Pohl et al.   ACS Synthetic Biology

CRISPR/Cas9-Based Efficient Genome Editing in Clostridium ljungdahlii, an Autotrophic Gas-Fermenting Bacterium

(2016) He Huang et al.   ACS Synthetic Biology

Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi)

(2016) Sara Cleto et al.   ACS Synthetic Biology

Myxobacteria: Moving, Killing, Feeding, and Surviving Together

(2016) José Muñoz-Dorado et al.   Frontiers in Microbiology

Sequence features associated with the cleavage efficiency of CRISPR/Cas9 system

(2016) Xiaoxi Liu et al.   Scientific Reports

Heterologous Production of the Marine Myxobacterial Antibiotic Haliangicin and Its Unnatural Analogues Generated by Engineering of the Biochemical Pathway

(2016) Yuwei Sun et al.   Scientific Reports

Multigene disruption in undomesticated Bacillus subtilis ATCC 6051a using the CRISPR/Cas9 system

(2016) Kang Zhang et al.   Scientific Reports

One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces

(2015) He Huang et al.   ACTA BIOCHIMICA ET BIOPHYSICA SINICA

Highly efficient editing of the actinorhodin polyketide chain length factor gene in Streptomyces coelicolor M145 using CRISPR/Cas9-CodA(sm) combined system

(2015) Hu Zeng et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Sequence determinants of improved CRISPR sgRNA design

(2015) Han Xu et al.   GENOME RESEARCH

CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo

(2015) Miguel A Moreno-Mateos et al.   NATURE METHODS

antiSMASH 3.0—a comprehensive resource for the genome mining of biosynthetic gene clusters

(2015) Tilmann Weber et al.   NUCLEIC ACIDS RESEARCH

Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system

(2015) Kabin Xie et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

(2014) Samuel H. Sternberg et al.   NATURE

Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation

(2014) John G Doench et al.   NATURE BIOTECHNOLOGY

CRISPR–Cas9-assisted recombineering in Lactobacillus reuteri

(2014) Jee-Hwan Oh et al.   NUCLEIC ACIDS RESEARCH

Efficient Mutagenesis by Cas9 Protein-Mediated Oligonucleotide Insertion and Large-Scale Assessment of Single-Guide RNAs

(2014) James A. Gagnon et al.   PLoS One

Protospacer Adjacent Motif (PAM)-Distal Sequences Engage CRISPR Cas9 DNA Target Cleavage

(2014) Regina Cencic et al.   PLoS One

High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System

(2014) Ryan E. Cobb et al.   ACS Synthetic Biology

Enhanced Specificity and Efficiency of the CRISPR/Cas9 System with Optimized sgRNA Parameters in Drosophila

(2014) Xingjie Ren et al.   Cell Reports

Future potential for anti-infectives from bacteria – How to exploit biodiversity and genomic potential

(2013) Rolf Müller et al.   INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Genetic Screens in Human Cells Using the CRISPR-Cas9 System

(2013) Tim Wang et al.   SCIENCE

Comprehensive Set of Integrative Plasmid Vectors for Copper-Inducible Gene Expression in Myxococcus xanthus

(2012) Nuria Gómez-Santos et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Genome Sequence of the Halotolerant Marine Bacterium Myxococcus fulvus HW-1

(2011) Z.-F. Li et al.   JOURNAL OF BACTERIOLOGY

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

(2011) Elitza Deltcheva et al.   NATURE

The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli

(2011) Rimantas Sapranauskas et al.   NUCLEIC ACIDS RESEARCH

Heterologous Expression of the Oxytetracycline Biosynthetic Pathway in Myxococcus xanthus

(2010) D. C. Stevens et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Myxobacteria, Polarity, and Multicellular Morphogenesis

(2010) D. Kaiser et al.   Cold Spring Harbor Perspectives in Biology

Myxobacteria—‘microbial factories’ for the production of bioactive secondary metabolites

(2009) Silke C. Wenzel et al.   Molecular BioSystems

Discovering the Hidden Secondary Metabolome of Myxococcus xanthus: a Study of Intraspecific Diversity

(2008) D. Krug et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Efficient transfer of two large secondary metabolite pathway gene clusters into heterologous hosts by transposition

(2008) Jun Fu et al.   NUCLEIC ACIDS RESEARCH

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

(2008) Carola Engler et al.   PLoS One

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE

CRISPR Interference Limits Horizontal Gene Transfer in Staphylococci by Targeting DNA

(2008) Luciano A. Marraffini et al.   SCIENCE