Probing the structure and function of acyl carrier proteins to unlock the strategic redesign of type II polyketide biosynthetic pathways

Structural basis for selectivity in a highly reducing type II polyketide synthase

(2020) Danyao Du et al.   Nature Chemical Biology

Gating mechanism of elongating β-ketoacyl-ACP synthases

(2020) Jeffrey T. Mindrebo et al.   Nature Communications

The Ketosynthase Domain Constrains the Design of Polyketide Synthases

(2020) Maja Klaus et al.   ACS Chemical Biology

Structural Characterization of Complex of Adenylation Domain and Carrier Protein by Using Pantetheine Cross-Linking Probe

(2020) Akimasa Miyanaga et al.   ACS Chemical Biology

Structural snapshots of the minimal PKS system responsible for octaketide biosynthesis

(2020) Alois Bräuer et al.   Nature Chemistry

Activity Mapping the Acyl Carrier Protein: Elongating Ketosynthase Interaction in Fatty Acid Biosynthesis

(2020) Jeffrey T. Mindrebo et al.   BIOCHEMISTRY

Widening the bottleneck: Heterologous expression, purification, and characterization of the Ktedonobacter racemifer minimal type II polyketide synthase in Escherichia coli

(2020) Joshua G. Klein et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Structure and Mechanism of the Ketosynthase-Chain Length Factor Didomain from a Prototypical Polyunsaturated Fatty Acid Synthase

(2020) Omar Santín et al.   BIOCHEMISTRY

‘It will change everything’: DeepMind’s AI makes gigantic leap in solving protein structures

(2020) Ewen Callaway   NATURE

Structural and dynamical rationale for fatty acid unsaturation in Escherichia coli

(2019) Greg J. Dodge et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineering of Chimeric Polyketide Synthases Using SYNZIP Docking Domains

(2019) Maja Klaus et al.   ACS Chemical Biology

One atom matters: modifying the thioester linkage affects structure of the acyl carrier protein

(2019) Terra Sztain et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Control of beta-Branching in Kalimantacin Biosynthesis: Application of Direct Observe 13C NMR to Polyketide Programming.

(2019) Paul D Walker et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Molecular basis for interactions between an acyl carrier protein and a ketosynthase

(2019) Jacob C. Milligan et al.   Nature Chemical Biology

Structure, function and dynamics in acyl carrier proteins

(2019) Rohit Farmer et al.   PLoS One

Tracking carrier protein motions with Raman spectroscopy

(2019) Samuel C. Epstein et al.   Nature Communications

Assembling a plug-and-play production line for combinatorial biosynthesis of aromatic polyketides in Escherichia coli

(2019) Matthew Cummings et al.   PLOS BIOLOGY

Colorimetric Assay Reports on Acyl Carrier Protein Interactions

(2019) Kofi K. Acheampong et al.   Scientific Reports

A Light‐Activated Acyl Carrier Protein “Trap” for Intermediate Capture in Type II Iterative Polyketide Biocatalysis

(2019) Samantha L. Kilgour et al.   CHEMISTRY-A EUROPEAN JOURNAL

The Structural Enzymology of Iterative Aromatic Polyketide Synthases: A Critical Comparison with Fatty Acid Synthases

(2018) Shiou-Chuan (Sheryl) Tsai   Annual Review of Biochemistry

An Oxetane-Based Polyketide Surrogate To Probe Substrate Binding in a Polyketide Synthase

(2018) Bryan D. Ellis et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Engineering strategies for rational polyketide synthase design

(2018) Maja Klaus et al.   NATURAL PRODUCT REPORTS

The Effect of Divalent Cations on the Thermostability of Type II Polyketide Synthase Acyl Carrier Proteins

(2018) Marco Rivas et al.   AICHE JOURNAL

Type II fatty acid and polyketide synthases: deciphering protein–protein and protein–substrate interactions

(2018) Aochiu Chen et al.   NATURAL PRODUCT REPORTS

Trapping interactions between catalytic domains and carrier proteins of modular biosynthetic enzymes with chemical probes

(2018) Andrew M. Gulick et al.   NATURAL PRODUCT REPORTS

Trapping the Complex Molecular Machinery of Polyketide and Fatty Acid Synthases with Tunable Silylcyanohydrin Crosslinkers

(2018) Sho Konno et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Polyketide mimetics yield structural and mechanistic insights into product template domain function in nonreducing polyketide synthases

(2017) Jesus F. Barajas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Sticky swinging arm dynamics: studies of an acyl carrier protein domain from the mycolactone polyketide synthase

(2016) S. Vance et al.   BIOCHEMICAL JOURNAL

Structure and mechanism of assembly line polyketide synthases

(2016) Thomas Robbins et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Evolutionary distinctiveness of fatty acid and polyketide synthesis in eukaryotes

(2016) Gurjeet S Kohli et al.   ISME Journal

Recognition of acyl carrier proteins by ketoreductases in assembly line polyketide synthases

(2016) Matthew P Ostrowski et al.   JOURNAL OF ANTIBIOTICS

Protein-Protein Interactions, Not Substrate Recognition, Dominate the Turnover of Chimeric Assembly Line Polyketide Synthases

(2016) Maja Klaus et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Recognition of extended linear and cyclised polyketide mimics by a type II acyl carrier protein

(2016) Xu Dong et al.   Chemical Science

Structural Insights into Anthranilate Priming during Type II Polyketide Biosynthesis

(2015) David R. Jackson et al.   ACS Chemical Biology

Architecture of the polyketide synthase module: surprises from electron cryo-microscopy

(2015) Janet L Smith et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

The Conserved Modular Elements of the Acyl Carrier Proteins of Lipid Synthesis Are Only Partially Interchangeable

(2015) Lei Zhu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Harnessing natural product assembly lines: structure, promiscuity, and engineering

(2015) Christopher C. Ladner et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Fatty acid biosynthesis revisited: structure elucidation and metabolic engineering

(2015) Joris Beld et al.   Molecular BioSystems

Evolution of chemical diversity by coordinated gene swaps in type II polyketide gene clusters

(2015) Maureen E. Hillenmeyer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Specific disulfide cross-linking to constrict the mobile carrier domain of nonribosomal peptide synthetases

(2015) M. J. Tarry et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Decoding and reprogramming complex polyketide assembly lines: prospects for synthetic biology

(2015) Christian Hertweck   TRENDS IN BIOCHEMICAL SCIENCES

The Structure of a Transient Complex of a Nonribosomal Peptide Synthetase and a Cytochrome P450 Monooxygenase

(2014) Kristina Haslinger et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

The chain-flipping mechanism of ACP (acyl carrier protein)-dependent enzymes appears universal

(2014) John E. Cronan   BIOCHEMICAL JOURNAL

Assembly Line Polyketide Synthases: Mechanistic Insights and Unsolved Problems

(2014) Chaitan Khosla et al.   BIOCHEMISTRY

Mapping a Ketosynthase:Acyl Carrier Protein Binding Interface via Unnatural Amino Acid-Mediated Photo-Cross-Linking

(2014) Zhixia Ye et al.   BIOCHEMISTRY

Modeling Linear and Cyclic PKS Intermediates through Atom Replacement

(2014) Gaurav Shakya et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Probing the Selectivity and Protein⋅Protein Interactions of a Nonreducing Fungal Polyketide Synthase Using Mechanism-Based Crosslinkers

(2013) Joel Bruegger et al.   CHEMISTRY & BIOLOGY

Sulfonyl 3-Alkynyl Pantetheinamides as Mechanism-Based Cross-Linkers of Acyl Carrier Protein Dehydratase

(2013) Fumihiro Ishikawa et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The phosphopantetheinyl transferases: catalysis of a post-translational modification crucial for life

(2013) Joris Beld et al.   NATURAL PRODUCT REPORTS

Chasing acyl carrier protein through a catalytic cycle of lipid A production

(2013) Ali Masoudi et al.   NATURE

Trapping the dynamic acyl carrier protein in fatty acid biosynthesis

(2013) Chi Nguyen et al.   NATURE

Structure and noncanonical chemistry of nonribosomal peptide biosynthetic machinery

(2012) Heather L. Condurso et al.   NATURAL PRODUCT REPORTS

Insights from the sea: Structural biology of marine polyketide synthases

(2012) David L. Akey et al.   NATURAL PRODUCT REPORTS

The structural role of the carrier protein – active controller or passive carrier

(2012) John Crosby et al.   NATURAL PRODUCT REPORTS

Reprogramming a module of the 6-deoxyerythronolide B synthase for iterative chain elongation

(2012) S. Kapur et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Acyl carrier protein structural classification and normal mode analysis

(2012) David C. Cantu et al.   PROTEIN SCIENCE

Binding and pKaModulation of a Polycyclic Substrate Analogue in a Type II Polyketide Acyl Carrier Protein

(2011) Robert W. Haushalter et al.   ACS Chemical Biology

Dehydratase-Specific Probes for Fatty Acid and Polyketide Synthases

(2011) Fumihiro Ishikawa et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Probing the interactions of an acyl carrier protein domain from the 6-deoxyerythronolide B synthase

(2011) Louise K. Charkoudian et al.   PROTEIN SCIENCE

Recognition of Intermediate Functionality by Acyl Carrier Protein over a Complete Cycle of Fatty Acid Biosynthesis

(2010) Eliza Płoskoń et al.   CHEMISTRY & BIOLOGY

Molecular recognition between ketosynthase and acyl carrier protein domains of the 6-deoxyerythronolide B synthase

(2010) S. Kapur et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Biosynthesis of Aromatic Polyketides in Bacteria

(2009) Abhirup Das et al.   ACCOUNTS OF CHEMICAL RESEARCH

Structural Basis for Binding Specificity between Subclasses of Modular Polyketide Synthase Docking Domains

(2009) Tonia J. Buchholz et al.   ACS Chemical Biology

The Biosynthetic Logic of Polyketide Diversity

(2009) Christian Hertweck   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Protein−Protein Recognition between Acyltransferases and Acyl Carrier Proteins in Multimodular Polyketide Synthases

(2009) Fong T. Wong et al.   BIOCHEMISTRY

The chemical biology of modular biosynthetic enzymes

(2009) Jordan L. Meier et al.   CHEMICAL SOCIETY REVIEWS

Probing the Interactions of Early Polyketide Intermediates with the Actinorhodin ACP from S. coelicolor A3(2)

(2009) Simon E. Evans et al.   JOURNAL OF MOLECULAR BIOLOGY

Emergent gene order in a model of modular polyketide synthases

(2009) B. Callahan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Antibiotics for Emerging Pathogens

(2009) M. A. Fischbach et al.   SCIENCE

Inhibition Kinetics and Emodin Cocrystal Structure of a Type II Polyketide Ketoreductase†,‡

(2008) Tyler Paz Korman et al.   BIOCHEMISTRY

An ACP Structural Switch: Conformational Differences between the Apo and Holo Forms of the Actinorhodin Polyketide Synthase Acyl Carrier Protein

(2008) Simon E. Evans et al.   CHEMBIOCHEM

Probing the Compatibility of Type II Ketosynthase-Carrier Protein Partners

(2008) Andrew S. Worthington et al.   CHEMBIOCHEM

Molecular Dynamics Simulations of the Apo-, Holo-, and Acyl-forms ofEscherichia coliAcyl Carrier Protein

(2008) David I. Chan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY