Generation of mouse conditional knockout alleles in one step using the i-GONAD method

Pervasive functional translation of noncanonical human open reading frames

(2020) Jin Chen et al.   SCIENCE

Sequential i-GONAD: An Improved In Vivo Technique for CRISPR/Cas9-Based Genetic Manipulations in Mice

(2020) Masahiro Sato et al.   Cells

Improving the efficiency of precise genome editing with site-specific Cas9-oligonucleotide conjugates

(2020) Xinyu Ling et al.   Science Advances

Generating mouse models for biomedical research: technological advances

(2019) Channabasavaiah B. Gurumurthy et al.   Disease Models & Mechanisms

Methodologies for Improving HDR Efficiency

(2019) Mingjie Liu et al.   Frontiers in Genetics

Generation of hyperlipidemic rabbit models using multiple sgRNAs targeted CRISPR/Cas9 gene editing system

(2019) Tingting Yuan et al.   Lipids in Health and Disease

Creation of CRISPR-based germline-genome-engineered mice without ex vivo handling of zygotes by i-GONAD

(2019) Channabasavaiah B. Gurumurthy et al.   Nature Protocols

CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites

(2019) Rajeswari Jayavaradhan et al.   Nature Communications

CRISPR-Cas9 Gene Editing in Lizards through Microinjection of Unfertilized Oocytes

(2019) Ashley M. Rasys et al.   Cell Reports

Comparative analysis of single-stranded DNA donors to generate conditional null mouse alleles

(2018) Denise G. Lanza et al.   BMC BIOLOGY

CLICK: one-step generation of conditional knockout mice

(2018) Yoshiki Miyasaka et al.   BMC GENOMICS

Efficient mouse genome engineering by CRISPR-EZ technology

(2018) Andrew J Modzelewski et al.   Nature Protocols

Fusogenic micropeptide Myomixer is essential for satellite cell fusion and muscle regeneration

(2018) Pengpeng Bi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair

(2018) M. Charpentier et al.   Nature Communications

The CRISPR tool kit for genome editing and beyond

(2018) Mazhar Adli   Nature Communications

Electroporation of mice zygotes with dual guide RNA/Cas9 complexes for simple and efficient cloning-free genome editing

(2018) Marie Teixeira et al.   Scientific Reports

Single-cell transcriptomics of 20 mouse organs creates a Tabula Muris

(2018) et al.   NATURE

Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors

(2017) Hiromi Miura et al.   Nature Protocols

Single-cut genome editing restores dystrophin expression in a new mouse model of muscular dystrophy

(2017) Leonela Amoasii et al.   Science Translational Medicine

A small cassette enables conditional gene inactivation by CRISPR/Cas9

(2017) Paloma M. Guzzardo et al.   Scientific Reports

CRISPR/Cas9 in Genome Editing and Beyond

(2016) Haifeng Wang et al.   Annual Review of Biochemistry

Delivery of Cas9 Protein into Mouse Zygotes through a Series of Electroporation Dramatically Increases the Efficiency of Model Creation

(2016) Wenbo Wang et al.   Journal of Genetics and Genomics

Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA

(2016) Christopher D Richardson et al.   NATURE BIOTECHNOLOGY

ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes

(2016) Kazuto Yoshimi et al.   Nature Communications

Biallelic editing of a lamprey genome using the CRISPR/Cas9 system

(2016) Yao Zu et al.   Scientific Reports

CRISPR-Cas: New Tools for Genetic Manipulations from Bacterial Immunity Systems

(2015) Wenyan Jiang et al.   Annual Review of Microbiology

CRISPR/Cas9-mediated mutagenesis in the sea lamprey Petromyzon marinus: a powerful tool for understanding ancestral gene functions in vertebrates

(2015) T. Square et al.   DEVELOPMENT

Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining

(2015) Takeshi Maruyama et al.   NATURE BIOTECHNOLOGY

Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach

(2015) Raj Chari et al.   NATURE METHODS

CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer single-stranded DNA

(2015) Hiromi Miura et al.   Scientific Reports

GONAD: Genome-editing via Oviductal Nucleic Acids Delivery system: a novel microinjection independent genome engineering method in mice

(2015) Gou Takahashi et al.   Scientific Reports

Is mouse embryonic stem cell technology obsolete?

(2015) William C Skarnes   GENOME BIOLOGY

Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice

(2015) Tomomi Aida et al.   GENOME BIOLOGY

Simple Genome Editing of Rodent Intact Embryos by Electroporation

(2015) Takehito Kaneko et al.   PLoS One

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

(2014) S. Bae et al.   BIOINFORMATICS

CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling

(2014) Randall J. Platt et al.   CELL

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

Precise and efficient genome editing in zebrafish using the CRISPR/Cas9 system

(2014) U. Irion et al.   DEVELOPMENT

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

(2014) Xuebing Wu et al.   NATURE BIOTECHNOLOGY

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

An Efficient Genotyping Method for Genome-modified Animals and Human Cells Generated with CRISPR/Cas9 System

(2014) Xiaoxiao Zhu et al.   Scientific Reports

Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

(2014) Steven Lin et al.   eLife

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering

(2013) Hui Yang et al.   CELL

Mouse Models of Cancer

(2011) Dong-Joo Cheon et al.   Annual Review of Pathology-Mechanisms of Disease

A conditional knockout resource for the genome-wide study of mouse gene function

(2011) William C. Skarnes et al.   NATURE

The construction of transgenic and gene knockout/knockin mouse models of human disease

(2011) Alfred Doyle et al.   TRANSGENIC RESEARCH

Adult satellite cells and embryonic muscle progenitors have distinct genetic requirements

(2009) Christoph Lepper et al.   NATURE