Efficient mouse genome engineering by CRISPR-EZ technology

Genome editing via delivery of Cas9 ribonucleoprotein

(2017) Mark A. DeWitt et al.   METHODS

Unexpected mutations after CRISPR–Cas9 editing in vivo

(2017) Kellie A Schaefer et al.   NATURE METHODS

Targeted gene knock-in by CRISPR/Cas ribonucleoproteins in porcine zygotes

(2017) Ki-Eun Park et al.   Scientific Reports

Promoting Cas9 degradation reduces mosaic mutations in non-human primate embryos

(2017) Zhuchi Tu et al.   Scientific Reports

Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse

(2016) Masakazu Hashimoto et al.   DEVELOPMENTAL BIOLOGY

Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes

(2016) Sean Chen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Delivery of Cas9 Protein into Mouse Zygotes through a Series of Electroporation Dramatically Increases the Efficiency of Model Creation

(2016) Wenbo Wang et al.   Journal of Genetics and Genomics

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Targeted mutagenesis in mice by electroporation of Cpf1 ribonucleoproteins

(2016) Junho K Hur et al.   NATURE BIOTECHNOLOGY

Efficient gene targeting in mouse zygotes mediated by CRISPR/Cas9-protein

(2016) Chris J. Jung et al.   TRANSGENIC RESEARCH

Nucleosomes impede Cas9 access to DNA in vivo and in vitro

(2016) Max A Horlbeck et al.   eLife

Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice

(2016) Yoshiko Nakagawa et al.   Biology Open

Somatic cell reprogramming-free generation of genetically modified pigs

(2016) F. Tanihara et al.   Science Advances

Exome sequencing in the knockin mice generated using the CRISPR/Cas system

(2016) Kazuo Nakajima et al.   Scientific Reports

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

Sequence determinants of improved CRISPR sgRNA design

(2015) Han Xu et al.   GENOME RESEARCH

Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection

(2015) Xiquan Liang et al.   JOURNAL OF BIOTECHNOLOGY

Off-target mutations are rare in Cas9-modified mice

(2015) Vivek Iyer et al.   NATURE METHODS

CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo

(2015) Miguel A Moreno-Mateos et al.   NATURE METHODS

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

GONAD: Genome-editing via Oviductal Nucleic Acids Delivery system: a novel microinjection independent genome engineering method in mice

(2015) Gou Takahashi et al.   Scientific Reports

CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes

(2015) Puping Liang et al.   Protein & Cell

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

Is mouse embryonic stem cell technology obsolete?

(2015) William C Skarnes   GENOME BIOLOGY

Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice

(2015) Tomomi Aida et al.   GENOME BIOLOGY

Simple Genome Editing of Rodent Intact Embryos by Electroporation

(2015) Takehito Kaneko et al.   PLoS One

Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing

(2015) Masakazu Hashimoto et al.   Scientific Reports

A Mouse Geneticist's Practical Guide to CRISPR Applications

(2014) P. Singh et al.   GENETICS

Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo

(2014) John A Zuris et al.   NATURE BIOTECHNOLOGY

Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems

(2014) Akihiro Yasue et al.   Scientific Reports

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering

(2013) Hui Yang et al.   CELL

Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases

(2013) Y. H. Sung et al.   GENOME RESEARCH

Heritable gene targeting in the mouse and rat using a CRISPR-Cas system

(2013) Dali Li et al.   NATURE BIOTECHNOLOGY

Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease

(2013) Wataru Fujii et al.   NUCLEIC ACIDS RESEARCH

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Patterns of exon-intron architecture variation of genes in eukaryotic genomes

(2009) Liucun Zhu et al.   BMC GENOMICS

Targeted genome editing in human cells with zinc finger nucleases constructed via modular assembly

(2009) H. J. Kim et al.   GENOME RESEARCH

What is (Still not) Known of the Mechanism by Which Electroporation Mediates Gene Transfer and Expression in Cells and Tissues

(2008) Jean-Michel Escoffre et al.   MOLECULAR BIOTECHNOLOGY