Generating mouse models for biomedical research: technological advances

Response to “Unexpected mutations after CRISPR–Cas9 editing in vivo”

(2018) Reynald M Lescarbeau et al.   NATURE METHODS

Response to “Unexpected mutations after CRISPR–Cas9 editing in vivo”

(2018) Sang-Tae Kim et al.   NATURE METHODS

Response to “Unexpected mutations after CRISPR–Cas9 editing in vivo”

(2018) Lauryl M J Nutter et al.   NATURE METHODS

Response to “Unexpected mutations after CRISPR–Cas9 editing in vivo”

(2018) Christopher J Wilson et al.   NATURE METHODS

Genome editing for the reproduction and remedy of human diseases in mice

(2017) Satoshi Hara et al.   JOURNAL OF HUMAN GENETICS

Applications of the CRISPR-Cas9 system in kidney research

(2017) Yoshiki Higashijima et al.   KIDNEY INTERNATIONAL

Modeling human disease in rodents by CRISPR/Cas9 genome editing

(2017) Marie-Christine Birling et al.   MAMMALIAN GENOME

In vivo genome editing and organoid transplantation models of colorectal cancer and metastasis

(2017) Jatin Roper et al.   NATURE BIOTECHNOLOGY

Disease model discovery from 3,328 gene knockouts by The International Mouse Phenotyping Consortium

(2017) Terrence F Meehan et al.   NATURE GENETICS

Unexpected mutations after CRISPR–Cas9 editing in vivo

(2017) Kellie A Schaefer et al.   NATURE METHODS

Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors

(2017) Hiromi Miura et al.   Nature Protocols

To cleave or not to cleave: therapeutic gene editing with and without programmable nucleases

(2017) Tod M. Woolf et al.   NATURE REVIEWS DRUG DISCOVERY

A CRISPR Path to Engineering New Genetic Mouse Models for Cardiovascular ResearchHighlights

(2016) Joseph M. Miano et al.   ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY

Nucleic acids delivery methods for genome editing in zygotes and embryos: the old, the new, and the old-new

(2016) Masahiro Sato et al.   Biology Direct

Using the mouse to model human disease: increasing validity and reproducibility

(2016) Monica J. Justice et al.   Disease Models & Mechanisms

Using CRISPR/Cas to study gene function and model diseasein vivo

(2016) Darjus F. Tschaharganeh et al.   FEBS Journal

CRISPR: a versatile tool for both forward and reverse genetics research

(2016) Channabasavaiah B. Gurumurthy et al.   HUMAN GENETICS

Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes

(2016) Sean Chen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Rapid phenotyping of knockout mice to identify genetic determinants of bone strength

(2016) Bernard Freudenthal et al.   JOURNAL OF ENDOCRINOLOGY

High-throughput discovery of novel developmental phenotypes

(2016) Mary E. Dickinson et al.   NATURE

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Animal-based studies will be essential for precision medicine

(2016) K. C. Kent Lloyd et al.   Science Translational Medicine

ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes

(2016) Kazuto Yoshimi et al.   Nature Communications

CRISPR/Cas9: Implications for Modeling and Therapy of Neurodegenerative Diseases

(2016) Weili Yang et al.   Frontiers in Molecular Neuroscience

Establishment of Nephrin Reporter Mice and Use for Chemical Screening

(2016) Junichi Tsuchida et al.   PLoS One

One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT)

(2015) Masato Ohtsuka et al.   BMC GENOMICS

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

Beyond knockouts: the International Knockout Mouse Consortium delivers modular and evolving tools for investigating mammalian genes

(2015) B. Rosen et al.   MAMMALIAN GENOME

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

(2015) Sergey Shmakov et al.   MOLECULAR CELL

Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining

(2015) Takeshi Maruyama et al.   NATURE BIOTECHNOLOGY

Therapeutic genome editing: prospects and challenges

(2015) David Benjamin Turitz Cox et al.   NATURE MEDICINE

Off-target mutations are rare in Cas9-modified mice

(2015) Vivek Iyer et al.   NATURE METHODS

MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems

(2015) Tetsushi Sakuma et al.   Nature Protocols

Transgenic Mice for Intersectional Targeting of Neural Sensors and Effectors with High Specificity and Performance

(2015) Linda Madisen et al.   NEURON

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Modeling Disease In Vivo With CRISPR/Cas9

(2015) Lukas E. Dow   TRENDS IN MOLECULAR MEDICINE

CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer single-stranded DNA

(2015) Hiromi Miura et al.   Scientific Reports

GONAD: Genome-editing via Oviductal Nucleic Acids Delivery system: a novel microinjection independent genome engineering method in mice

(2015) Gou Takahashi et al.   Scientific Reports

Precision cancer mouse models through genome editing with CRISPR-Cas9

(2015) Haiwei Mou et al.   Genome Medicine

Applying the ARRIVE Guidelines to an In Vivo Database

(2015) Natasha A. Karp et al.   PLOS BIOLOGY

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

Is mouse embryonic stem cell technology obsolete?

(2015) William C Skarnes   GENOME BIOLOGY

Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice

(2015) Tomomi Aida et al.   GENOME BIOLOGY

Insertion of sequences at the original provirus integration site of mouseROSA26 locus using the CRISPR/Cas9 system

(2015) Rolen M. Quadros et al.   FEBS Open Bio

Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing

(2015) Masakazu Hashimoto et al.   Scientific Reports

CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling

(2014) Randall J. Platt et al.   CELL

Somatic mosaicism and allele complexity induced by CRISPR/Cas9 RNA injections in mouse zygotes

(2014) Shuo-Ting Yen et al.   DEVELOPMENTAL BIOLOGY

Pax5 loss imposes a reversible differentiation block in B-progenitor acute lymphoblastic leukemia

(2014) G. J. Liu et al.   GENES & DEVELOPMENT

A conditional mutant allele for analysis ofMixl1function in the mouse

(2014) Maria V. Pulina et al.   GENESIS

Preclinical research: Make mouse studies work

(2014) Steve Perrin   NATURE

CRISPR-Cas systems for editing, regulating and targeting genomes

(2014) Jeffry D Sander et al.   NATURE BIOTECHNOLOGY

Novel skin phenotypes revealed by a genome-wide mouse reverse genetic screen

(2014) Kifayathullah Liakath-Ali et al.   Nature Communications

Rapid generation of mouse models with defined point mutations by the CRISPR/Cas9 system

(2014) Masafumi Inui et al.   Scientific Reports

Efficient genetic manipulation of the NOD-Rag1-/-IL2RgammaC-null mouse by combining in vitro fertilization and CRISPR/Cas9 technology

(2014) Feng Li et al.   Scientific Reports

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

Generation of gene-modified mice via Cas9/RNA-mediated gene targeting

(2013) Bin Shen et al.   CELL RESEARCH

Genome editing and animal models

(2013) Ruby Yanru Chen-Tsai et al.   CHINESE SCIENCE BULLETIN

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

(2013) Seung Woo Cho et al.   NATURE BIOTECHNOLOGY

Knockout mice created by TALEN-mediated gene targeting

(2013) Young Hoon Sung et al.   NATURE BIOTECHNOLOGY

Heritable gene targeting in the mouse and rat using a CRISPR-Cas system

(2013) Dali Li et al.   NATURE BIOTECHNOLOGY

Conditionals by inversion provide a universal method for the generation of conditional alleles

(2013) Aris N. Economides et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

RNA-programmed genome editing in human cells

(2013) Martin Jinek et al.   eLife

In Vivo RNAi-Based Screens: Studies in Model Organisms

(2013) Miki Yamamoto-Hino et al.   Genes

PITT: Pronuclear Injection-Based Targeted Transgenesis, a Reliable Transgene Expression Method in Mice

(2012) Masato OHTSUKA et al.   EXPERIMENTAL ANIMALS

Dll4–Notch signaling in Flt3-independent dendritic cell development and autoimmunity in mice

(2012) Fabienne Billiard et al.   JOURNAL OF EXPERIMENTAL MEDICINE

The mammalian gene function resource: the international knockout mouse consortium

(2012) Allan Bradley et al.   MAMMALIAN GENOME

Complex reorganization and predominant non-homologous repair following chromosomal breakage in karyotypically balanced germline rearrangements and transgenic integration

(2012) Colby Chiang et al.   NATURE GENETICS

TALENs: a widely applicable technology for targeted genome editing

(2012) J. Keith Joung et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Targeting Synthetic Lethal Interactions between Myc and the eIF4F Complex Impedes Tumorigenesis

(2012) Chen-Ju Lin et al.   Cell Reports

Human Genome Sequencing in Health and Disease

(2011) Claudia Gonzaga-Jauregui et al.   Annual Review of Medicine

A Rapid and Scalable System for Studying Gene Function in Mice Using Conditional RNA Interference

(2011) Prem K. Premsrirut et al.   CELL

Technical approaches for mouse models of human disease

(2011) M. J. Justice et al.   Disease Models & Mechanisms

Genome Engineering With Zinc-Finger Nucleases

(2011) Dana Carroll   GENETICS

Genetic background effects on age-related hearing loss associated with Cdh23 variants in mice

(2011) Kelly L. Kane et al.   HEARING RESEARCH

A conditional knockout resource for the genome-wide study of mouse gene function

(2011) William C. Skarnes et al.   NATURE

Multiple new site-specific recombinases for use in manipulating animal genomes

(2011) A. Nern et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Site-specific integrase-mediated transgenesis in mice via pronuclear injection

(2011) B. Tasic et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Targeted transgenesis through pronuclear injection of improved vectors into in vitro fertilized eggs

(2011) Masato Ohtsuka et al.   TRANSGENIC RESEARCH

Targeted Genome Modification in Mice Using Zinc-Finger Nucleases

(2010) I. D. Carbery et al.   GENETICS

Genome editing with engineered zinc finger nucleases

(2010) Fyodor D. Urnov et al.   NATURE REVIEWS GENETICS

Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression

(2010) Masato Ohtsuka et al.   NUCLEIC ACIDS RESEARCH

Improving Bioscience Research Reporting: The ARRIVE Guidelines for Reporting Animal Research

(2010) Carol Kilkenny et al.   PLOS BIOLOGY

The basal-like mammary carcinomas induced by Brca1 or Bard1 inactivation implicate the BRCA1/BARD1 heterodimer in tumor suppression

(2008) R. Shakya et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Extensive genomic copy number variation in embryonic stem cells

(2008) Q. Liang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Artificial miRNAs mitigate shRNA-mediated toxicity in the brain: Implications for the therapeutic development of RNAi

(2008) J. L. McBride et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Conditional transgenic mouse models: from the basics to genome-wide sets of knockouts and current studies of tissue regeneration

(2008) Ernesto Bockamp et al.   Regenerative Medicine