Comparative analysis of single-stranded DNA donors to generate conditional null mouse alleles

Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

(2018) Gemma F. Codner et al.   BMC BIOLOGY

CRISPR/Cas9-mediated deletion of lncRNA Gm26878 in the distant Foxf1 enhancer region

(2017) Przemyslaw Szafranski et al.   MAMMALIAN GENOME

One-step generation of conditional and reversible gene knockouts

(2017) Amanda Andersson-Rolf et al.   NATURE METHODS

Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors

(2017) Hiromi Miura et al.   Nature Protocols

CRISPR/Cas9-mediated gene manipulation to create single-amino-acid-substituted and floxed mice with a cloning-free method

(2017) Xiaolong Ma et al.   Scientific Reports

Optimization of the production of knock-in alleles by CRISPR/Cas9 microinjection into the mouse zygote

(2017) Aurélien Raveux et al.   Scientific Reports

A Convenient Cas9-based Conditional Knockout Strategy for Simultaneously Targeting Multiple Genes in Mouse

(2017) Jiang Chen et al.   Scientific Reports

Efficient generation of conditional knockout mice via sequential introduction of lox sites

(2017) Takuro Horii et al.   Scientific Reports

Revealing hidden complexities of genomic rearrangements generated with Cas9

(2017) Katharina Boroviak et al.   Scientific Reports

A CRISPR Path to Engineering New Genetic Mouse Models for Cardiovascular ResearchHighlights

(2016) Joseph M. Miano et al.   ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY

Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes

(2016) Van Trung Chu et al.   BMC BIOTECHNOLOGY

Chromosome engineering in zygotes with CRISPR/Cas9

(2016) Katharina Boroviak et al.   GENESIS

Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA

(2016) Christopher D Richardson et al.   NATURE BIOTECHNOLOGY

Efficient gene targeting in mouse zygotes mediated by CRISPR/Cas9-protein

(2016) Chris J. Jung et al.   TRANSGENIC RESEARCH

ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes

(2016) Kazuto Yoshimi et al.   Nature Communications

CRISPR/Cas9-Mediated Insertion of loxP Sites in the Mouse Dock7 Gene Provides an Effective Alternative to Use of Targeted Embryonic Stem Cells

(2016) Kathleen A. Bishop et al.   G3-Genes Genomes Genetics

Analyses of point mutation repair and allelic heterogeneity generated by CRISPR/Cas9 and single-stranded DNA oligonucleotides

(2016) Pawel Bialk et al.   Scientific Reports

Exome sequencing in the knockin mice generated using the CRISPR/Cas system

(2016) Kazuo Nakajima et al.   Scientific Reports

A mouse informatics platform for phenotypic and translational discovery

(2015) Natalie Ring et al.   MAMMALIAN GENOME

Off-target mutations are rare in Cas9-modified mice

(2015) Vivek Iyer et al.   NATURE METHODS

CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer single-stranded DNA

(2015) Hiromi Miura et al.   Scientific Reports

Genome Engineering with Targetable Nucleases

(2014) Dana Carroll   Annual Review of Biochemistry

A Mouse Geneticist's Practical Guide to CRISPR Applications

(2014) P. Singh et al.   GENETICS

CRISPR-Cas systems for editing, regulating and targeting genomes

(2014) Jeffry D Sander et al.   NATURE BIOTECHNOLOGY

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR–Cas platform

(2014) K. Yoshimi et al.   Nature Communications

Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering

(2014) Takuro Horii et al.   Scientific Reports

Conditional targeting of Ispd using paired Cas9 nickase and a single DNA template in mice

(2014) Angus Yiu-fai Lee et al.   FEBS Open Bio

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering

(2013) Hui Yang et al.   CELL

Correction of a Genetic Disease in Mouse via Use of CRISPR-Cas9

(2013) Yuxuan Wu et al.   Cell Stem Cell

Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases

(2013) Y. H. Sung et al.   GENOME RESEARCH

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems

(2013) Wei Li et al.   NATURE BIOTECHNOLOGY

Heritable gene targeting in the mouse and rat using a CRISPR-Cas system

(2013) Dali Li et al.   NATURE BIOTECHNOLOGY

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9 System

(2013) Andrew R. Bassett et al.   Cell Reports

Towards an encyclopaedia of mammalian gene function: the International Mouse Phenotyping Consortium

(2012) S. D. M. Brown et al.   Disease Models & Mechanisms

The mammalian gene function resource: the international knockout mouse consortium

(2012) Allan Bradley et al.   MAMMALIAN GENOME

A conditional knockout resource for the genome-wide study of mouse gene function

(2011) William C. Skarnes et al.   NATURE

Accurate and objective copy number profiling using real-time quantitative PCR

(2010) Barbara D’haene et al.   METHODS