Studying Phase Separation in Confinement

Multiphase Complex Coacervate Droplets

(2020) Tiemei Lu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

pH-Controlled Coacervate–Membrane Interactions within Liposomes

(2020) Mart G. F. Last et al.   ACS Nano

Making the Case for Disordered Proteins and Biomolecular Condensates in Bacteria

(2020) Megan C. Cohan et al.   TRENDS IN BIOCHEMICAL SCIENCES

Complex microparticle architectures from stimuli-responsive intrinsically disordered proteins

(2020) Stefan Roberts et al.   Nature Communications

Clusters of bacterial RNA polymerase are biomolecular condensates that assemble through liquid–liquid phase separation

(2020) Anne-Marie Ladouceur et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A molecular assembly phase transition and kinetic proofreading modulate Ras activation by SOS

(2019) William Y. C. Huang et al.   SCIENCE

Stoichiometry controls activity of phase-separated clusters of actin signaling proteins

(2019) Lindsay B. Case et al.   SCIENCE

Liquid–liquid phase separation during amphiphilic self-assembly

(2019) Alessandro Ianiro et al.   Nature Chemistry

Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates

(2019) Simon Alberti et al.   CELL

Spatial Positioning and Chemical Coupling in Coacervate-in-Proteinosome Protocells

(2019) Richard Booth et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Spatiotemporal control of coacervate formation within liposomes

(2019) Siddharth Deshpande et al.   Nature Communications

Understanding the Coacervate-to-Vesicle Transition of Globular Fusion Proteins to Engineer Protein Vesicle Size and Membrane Heterogeneity

(2019) Yeongseon Jang et al.   BIOMACROMOLECULES

Phase Separation of Zonula Occludens Proteins Drives Formation of Tight Junctions

(2019) Oliver Beutel et al.   CELL

Functional Implications of Intracellular Phase Transitions

(2018) Alex S. Holehouse et al.   BIOCHEMISTRY

Macromolecularly Crowded Protocells from Reversibly Shrinking Monodisperse Liposomes

(2018) Nan-Nan Deng et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Imaging dynamic and selective low-complexity domain interactions that control gene transcription

(2018) Shasha Chong et al.   SCIENCE

Coactivator condensation at super-enhancers links phase separation and gene control

(2018) Benjamin R. Sabari et al.   SCIENCE

Mediator and RNA polymerase II clusters associate in transcription-dependent condensates

(2018) Won-Ki Cho et al.   SCIENCE

Liquid–liquid phase separation in artificial cells

(2018) Charles D. Crowe et al.   Interface Focus

Hierarchical Self-Assembly of a Copolymer-Stabilized Coacervate Protocell

(2017) Alexander F. Mason et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Phase separation drives heterochromatin domain formation

(2017) Amy R. Strom et al.   NATURE

Programming molecular self-assembly of intrinsically disordered proteins containing sequences of low complexity

(2017) Joseph R. Simon et al.   Nature Chemistry

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Liquid phase condensation in cell physiology and disease

(2017) Yongdae Shin et al.   SCIENCE

Engineering Globular Protein Vesicles through Tunable Self-Assembly of Recombinant Fusion Proteins

(2017) Yeongseon Jang et al.   Small

Mechanisms and Consequences of Macromolecular Phase Separation

(2016) Louis-Philippe Bergeron-Sandoval et al.   CELL

Liquid–liquid phase separation in cellular signaling systems

(2016) P Andrew Chong et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

RNA-Based Coacervates as a Model for Membraneless Organelles: Formation, Properties, and Interfacial Liposome Assembly

(2016) William M. Aumiller et al.   LANGMUIR

Sequence Determinants of Intracellular Phase Separation by Complex Coacervation of a Disordered Protein

(2016) Chi W. Pak et al.   MOLECULAR CELL

Growth and division of active droplets provides a model for protocells

(2016) David Zwicker et al.   Nature Physics

Phase separation of signaling molecules promotes T cell receptor signal transduction

(2016) Xiaolei Su et al.   SCIENCE

Phase Separation: Linking Cellular Compartmentalization to Disease

(2016) Adriano Aguzzi et al.   TRENDS IN CELL BIOLOGY

Octanol-assisted liposome assembly on chip

(2016) Siddharth Deshpande et al.   Nature Communications

Phase Separation by Low Complexity Domains Promotes Stress Granule Assembly and Drives Pathological Fibrillization

(2015) Amandine Molliex et al.   CELL

Polymer physics of intracellular phase transitions

(2015) Clifford P. Brangwynne et al.   Nature Physics

Liquid demixing of intrinsically disordered proteins is seeded by poly(ADP-ribose)

(2015) Matthias Altmeyer et al.   Nature Communications

Liquid-Liquid Phase Separation in Biology

(2014) Anthony A. Hyman et al.   Annual Review of Cell and Developmental Biology

Design and Construction of Higher-Order Structure and Function in Proteinosome-Based Protocells

(2014) Xin Huang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Thermally Triggered Self-Assembly of Folded Proteins into Vesicles

(2014) Won Min Park et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Nanoparticle-Based Membrane Assembly and Silicification in Coacervate Microdroplets as a Route to Complex Colloidosomes

(2014) James Fothergill et al.   LANGMUIR

Multiphase Water-in-Oil Emulsion Droplets for Cell-Free Transcription–Translation

(2014) Paola Torre et al.   LANGMUIR

Fatty acid membrane assembly on coacervate microdroplets as a step towards a hybrid protocell model

(2014) T-Y. Dora Tang et al.   Nature Chemistry

Spontaneous Structuration in Coacervate-Based Protocells by Polyoxometalate-Mediated Membrane Assembly

(2014) David S. Williams et al.   Small

A Unified Model for De Novo Design of Elastin-like Polypeptides with Tunable Inverse Transition Temperatures

(2013) Jonathan R. McDaniel et al.   BIOMACROMOLECULES

Enhanced transcription rates in membrane-free protocells formed by coacervation of cell lysate

(2013) E. Sokolova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Interfacial assembly of protein–polymer nano-conjugates into stimulus-responsive biomimetic protocells

(2013) Xin Huang et al.   Nature Communications

Getting RNA and Protein in Phase

(2012) Stephanie C. Weber et al.   CELL

Phase transitions in the assembly of multivalent signalling proteins

(2012) Pilong Li et al.   NATURE

Beyond Oil and Water--Phase Transitions in Cells

(2012) A. A. Hyman et al.   SCIENCE

Complete Budding and Asymmetric Division of Primitive Model Cells To Produce Daughter Vesicles with Different Interior and Membrane Compositions

(2011) Meghan Andes-Koback et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Perinuclear P granules are the principal sites of mRNA export in adult C. elegans germ cells

(2010) U. Sheth et al.   DEVELOPMENT

Spontaneous Formation of Giant Unilamellar Vesicles from Microdroplets of a Polyion Complex by Thermally Induced Phase Separation

(2009) Hidehiro Oana et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation

(2009) C. P. Brangwynne et al.   SCIENCE

Budding and Asymmetric Protein Microcompartmentation in Giant Vesicles Containing Two Aqueous Phases

(2007) M. Scott Long et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY