A molecular assembly phase transition and kinetic proofreading modulate Ras activation by SOS

Stoichiometry controls activity of phase-separated clusters of actin signaling proteins

(2019) Lindsay B. Case et al.   SCIENCE

Protein Clusters in Phosphotyrosine Signal Transduction

(2018) Bruce J. Mayer et al.   JOURNAL OF MOLECULAR BIOLOGY

The Interdependent Activation of Son-of-Sevenless and Ras

(2018) Pradeep Bandaru et al.   Cold Spring Harbor Perspectives in Medicine

Allosteric Modulation of Grb2 Recruitment to the Intrinsically Disordered Scaffold Protein, LAT, by Remote Site Phosphorylation

(2017) William Y. C. Huang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Mechanism of SOS PR-domain autoinhibition revealed by single-molecule assays on native protein from lysate

(2017) Young Kwang Lee et al.   Nature Communications

Design of a Split Intein with Exceptional Protein Splicing Activity

(2016) Adam J. Stevens et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

One-way membrane trafficking of SOS in receptor-triggered Ras activation

(2016) Sune M Christensen et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Phosphotyrosine-mediated LAT assembly on membranes drives kinetic bifurcation in recruitment dynamics of the Ras activator SOS

(2016) William Y. C. Huang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Phase separation of signaling molecules promotes T cell receptor signal transduction

(2016) Xiaolei Su et al.   SCIENCE

Conservation of protein abundance patterns reveals the regulatory architecture of the EGFR-MAPK pathway

(2016) Tujin Shi et al.   Science Signaling

One-way membrane trafficking of SOS in receptor-triggered Ras activation

(2016) Sune M Christensen et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

An electrostatic selection mechanism controls sequential kinase signaling downstream of the T cell receptor

(2016) Neel H Shah et al.   eLife

Conservation of protein abundance patterns reveals the regulatory architecture of the EGFR-MAPK pathway

(2016) Tujin Shi et al.   Science Signaling

Sub-100μs nanoimprint lithography at wafer scale

(2015) Massimo Tormen et al.   MICROELECTRONIC ENGINEERING

Single-Molecule Studies Reveal a Hidden Key Step in the Activation Mechanism of Membrane-Bound Protein Kinase C-α

(2014) Brian P. Ziemba et al.   BIOCHEMISTRY

H-Ras forms dimers on membrane surfaces via a protein-protein interface

(2014) W.-C. Lin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ras activation by SOS: Allosteric regulation by altered fluctuation dynamics

(2014) L. Iversen et al.   SCIENCE

Phase transitions of multivalent proteins can promote clustering of membrane receptors

(2014) Sudeep Banjade et al.   eLife

Extein Residues Play an Intimate Role in the Rate-Limiting Step of Protein Trans-Splicing

(2013) Neel H. Shah et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Structural Basis for Activation of ZAP-70 by Phosphorylation of the SH2-Kinase Linker

(2013) Q. Yan et al.   MOLECULAR AND CELLULAR BIOLOGY

The Ability of Sos1 to Oligomerize the Adaptor Protein LAT Is Separable from Its Guanine Nucleotide Exchange Activity in Vivo

(2013) R. L. Kortum et al.   Science Signaling

Multivalent Binding and Facilitated Diffusion Account for the Formation of the Grb2–Sos1 Signaling Complex in a Cooperative Manner

(2012) Caleb B. McDonald et al.   BIOCHEMISTRY

Pre-existing clusters of the adaptor Lat do not participate in early T cell signaling events

(2011) David J Williamson et al.   NATURE IMMUNOLOGY

Structural Basis for Activation and Inhibition of Class I Phosphoinositide 3-Kinases

(2011) Oscar Vadas et al.   Science Signaling

Molecular mechanisms in signal transduction at the membrane

(2010) Jay T Groves et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Role of the histone domain in the autoinhibition and activation of the Ras activator Son of Sevenless

(2010) Jodi Gureasko et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Allosteric gating of Son of sevenless activity by the histone domain

(2010) Kamlesh K. Yadav et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

T-Cell Signaling Regulated by the Tec Family Kinase, Itk

(2010) A. H. Andreotti et al.   Cold Spring Harbor Perspectives in Biology

Aggregation of Membrane Proteins by Cytosolic Cross-Linkers: Theory and Simulation of the LAT-Grb2-SOS1 System

(2009) Ambarish Nag et al.   BIOPHYSICAL JOURNAL

On the Mechanism of Trolox as Antiblinking and Antibleaching Reagent

(2009) Thorben Cordes et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Biophysical Mechanism for Ras-Nanocluster Formation and Signaling in Plasma Membrane

(2009) Thomas Gurry et al.   PLoS One

Kinetic Control of Histidine-Tagged Protein Surface Density on Supported Lipid Bilayers

(2008) Jeffrey A. Nye et al.   LANGMUIR

Membrane-dependent signal integration by the Ras activator Son of sevenless

(2008) Jodi Gureasko et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Mocr: A novel fusion tag for enhancing solubility that is compatible with structural biology applications

(2008) James DelProposto et al.   PROTEIN EXPRESSION AND PURIFICATION