Liquid–liquid phase separation in artificial cells

Physical Principles and Extant Biology Reveal Roles for RNA-Containing Membraneless Compartments in Origins of Life Chemistry

(2018) Raghav R. Poudyal et al.   BIOCHEMISTRY

A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins

(2018) Jie Wang et al.   CELL

Tailoring the appearance: what will synthetic cells look like?

(2018) Willem Kasper Spoelstra et al.   CURRENT OPINION IN BIOTECHNOLOGY

Oligonucleotide–Peptide Complexes: Phase Control by Hybridization

(2018) Jeffrey R. Vieregg et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Phase Behavior and Salt Partitioning in Polyelectrolyte Complex Coacervates

(2018) Lu Li et al.   MACROMOLECULES

Bacterial microcompartments

(2018) Cheryl A. Kerfeld et al.   NATURE REVIEWS MICROBIOLOGY

Controlling compartmentalization by non-membrane-bound organelles

(2018) Richard J. Wheeler et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Reversible generation of coacervate droplets in an enzymatic network

(2018) Karina K. Nakashima et al.   Soft Matter

Impact of macromolecular crowding on RNA/spermine complex coacervation and oligonucleotide compartmentalization

(2018) A. M. Marianelli et al.   Soft Matter

Origins of building blocks of life: A review

(2018) Norio Kitadai et al.   Geoscience Frontiers

Artificial cell mimics as simplified models for the study of cell biology

(2017) Ali Salehi-Reyhani et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

Sequence and entropy-based control of complex coacervates

(2017) Li-Wei Chang et al.   Nature Communications

Recent Progress in Micro/Nanoreactors toward the Creation of Artificial Organelles

(2017) Maria Godoy-Gallardo et al.   Advanced Healthcare Materials

Combining Catalytic Microparticles with Droplets Formed by Phase Coexistence: Adsorption and Activity of Natural Clays at the Aqueous/Aqueous Interface

(2017) Fatma Pir Cakmak et al.   Scientific Reports

Water-in-Water Droplets by Passive Microfluidic Flow Focusing

(2016) Byeong-Ui Moon et al.   ANALYTICAL CHEMISTRY

Aqueous two-phase system (ATPS): an overview and advances in its applications

(2016) Mujahid Iqbal et al.   BIOLOGICAL PROCEDURES ONLINE

Miniaturization of aqueous two-phase extraction for biological applications: From micro-tubes to microchannels

(2016) Ruben R. G. Soares et al.   Biotechnology Journal

Phase separation in biology; functional organization of a higher order

(2016) Diana M. Mitrea et al.   Cell Communication and Signaling

Water-in-water (W/W) emulsions

(2016) Jordi Esquena   CURRENT OPINION IN COLLOID & INTERFACE SCIENCE

Bottom-up Construction of a Primordial Carboxysome Mimic

(2016) Raphael Frey et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Microfluidic Assembly of Monodisperse Vesosomes as Artificial Cell Models

(2016) Nan-Nan Deng et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Polyamine/Nucleotide Coacervates Provide Strong Compartmentalization of Mg2+, Nucleotides, and RNA

(2016) Erica A. Frankel et al.   LANGMUIR

RNA-Based Coacervates as a Model for Membraneless Organelles: Formation, Properties, and Interfacial Liposome Assembly

(2016) William M. Aumiller et al.   LANGMUIR

Selective Uptake and Refolding of Globular Proteins in Coacervate Microdroplets

(2016) Nicolas Martin et al.   LANGMUIR

Phase-Separation-Induced Formation of Janus Droplets Based on Aqueous Two-Phase Systems

(2016) Hao Yuan et al.   MACROMOLECULAR CHEMISTRY AND PHYSICS

A simple physical mechanism enables homeostasis in primitive cells

(2016) Aaron E. Engelhart et al.   Nature Chemistry

Phasing in and out

(2016) James Shorter   Nature Chemistry

Engineering genetic circuit interactions within and between synthetic minimal cells

(2016) Katarzyna P. Adamala et al.   Nature Chemistry

Membraneless organelles can melt nucleic acid duplexes and act as biomolecular filters

(2016) Timothy J. Nott et al.   Nature Chemistry

Nonenzymatic biomimetic remodeling of phospholipids in synthetic liposomes

(2016) Roberto J. Brea et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Spatial colocalization and functional link of purinosomes with mitochondria

(2016) J. B. French et al.   SCIENCE

Complex coacervate-based materials for biomedicine

(2016) Whitney C. Blocher et al.   Wiley Interdisciplinary Reviews-Nanomedicine and Nanobiotechnology

Self-transformation and structural reconfiguration in coacervate-based protocells

(2016) Ravinash Krishna Kumar et al.   Chemical Science

Droplet-based microfluidics for artificial cell generation: a brief review

(2016) Chiara Martino et al.   Interface Focus

Stabilization of Water-in-Water Emulsions by Nanorods

(2016) Karthik R. Peddireddy et al.   ACS Macro Letters

Miniaturization of aqueous two-phase extraction for biological applications: From micro-tubes to microchannels

(2016) Ruben R. G. Soares et al.   Biotechnology Journal

Giant Vesicles Exposed to Aqueous Two-Phase Systems: Membrane Wetting, Budding Processes, and Spontaneous Tubulation

(2016) Rumiana Dimova et al.   Advanced Materials Interfaces

Microenvironments created by liquid-liquid phase transition control the dynamic distribution of bacterial division FtsZ protein

(2016) Begoña Monterroso et al.   Scientific Reports

Compartmentalization Approaches in Soft Matter Science: From Nanoreactor Development to Organelle Mimics

(2015) Lise Schoonen et al.   ADVANCED MATERIALS

Microfluidic Formation of Membrane-Free Aqueous Coacervate Droplets in Water

(2015) Dirk van Swaay et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Origin and evolution of metabolic sub-cellular compartmentalization in eukaryotes

(2015) Toni Gabaldón et al.   BIOCHIMIE

Colocalization and Sequential Enzyme Activity in Aqueous Biphasic Systems: Experiments and Modeling

(2015) Bradley W. Davis et al.   BIOPHYSICAL JOURNAL

In vitro gene expression within membrane-free coacervate protocells

(2015) T.-Y. Dora. Tang et al.   CHEMICAL COMMUNICATIONS

The RNA World as a Model System to Study the Origin of Life

(2015) Abe Pressman et al.   CURRENT BIOLOGY

Preparation and mechanical characterisation of giant unilamellar vesicles by a microfluidic method

(2015) K. Karamdad et al.   LAB ON A CHIP

Formation of Giant Unilamellar Proteo-Liposomes by Osmotic Shock

(2015) Isabelle Motta et al.   LANGMUIR

Aqueous Emulsion Droplets Stabilized by Lipid Vesicles as Microcompartments for Biomimetic Mineralization

(2015) David N. Cacace et al.   LANGMUIR

Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles

(2015) Timothy J. Nott et al.   MOLECULAR CELL

Phosphorylation-mediated RNA/peptide complex coacervation as a model for intracellular liquid organelles

(2015) William M. Aumiller et al.   Nature Chemistry

Sequence heuristics to encode phase behaviour in intrinsically disordered protein polymers

(2015) Felipe García Quiroz et al.   NATURE MATERIALS

The disordered P granule protein LAF-1 drives phase separation into droplets with tunable viscosity and dynamics

(2015) Shana Elbaum-Garfinkle et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Bioinspired membrane-based systems for a physical approach of cell organization and dynamics: usefulness and limitations

(2015) T. J. Lagny et al.   Interface Focus

Intrinsically Disordered Proteins and Intrinsically Disordered Protein Regions

(2014) Christopher J. Oldfield et al.   Annual Review of Biochemistry

Liquid-Liquid Phase Separation in Biology

(2014) Anthony A. Hyman et al.   Annual Review of Cell and Developmental Biology

Synthetic cellularity based on non-lipid micro-compartments and protocell models

(2014) Mei Li et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Interactions of Macromolecular Crowding Agents and Cosolutes with Small-Molecule Substrates: Effect on Horseradish Peroxidase Activity with Two Different Substrates

(2014) William M. Aumiller et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Coupled Enzyme Reactions Performed in Heterogeneous Reaction Media: Experiments and Modeling for Glucose Oxidase and Horseradish Peroxidase in a PEG/Citrate Aqueous Two-Phase System

(2014) William M. Aumiller et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Single-step microfluidic fabrication of soft monodisperse polyelectrolyte microcapsules by interfacial complexation

(2014) Gilad Kaufman et al.   LAB ON A CHIP

Multiphase Water-in-Oil Emulsion Droplets for Cell-Free Transcription–Translation

(2014) Paola Torre et al.   LANGMUIR

Fatty acid membrane assembly on coacervate microdroplets as a step towards a hybrid protocell model

(2014) T-Y. Dora Tang et al.   Nature Chemistry

Rapid RNA Exchange in Aqueous Two-Phase System and Coacervate Droplets

(2014) Tony Z. Jia et al.   ORIGINS OF LIFE AND EVOLUTION OF BIOSPHERES

Molecular-crowding effects on single-molecule RNA folding/unfolding thermodynamics and kinetics

(2014) N. F. Dupuis et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mesoporous silicon sponge as an anti-pulverization structure for high-performance lithium-ion battery anodes

(2014) Xiaolin Li et al.   Nature Communications

Vesicle-based artificial cells as chemical microreactors with spatially segregated reaction pathways

(2014) Yuval Elani et al.   Nature Communications

The Effect of Salt on the Complex Coacervation of Vinyl Polyelectrolytes

(2014) Sarah Perry et al.   Polymers

Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically disordered proteins in C. elegans

(2014) Jennifer T Wang et al.   eLife

All-aqueous multiphase microfluidics

(2013) Yang Song et al.   Biomicrofluidics

The purinosome, a multi-protein complex involved in the de novo biosynthesis of purines in humans

(2013) Hong Zhao et al.   CHEMICAL COMMUNICATIONS

Morphology and function of membrane-bound organelles

(2013) Rebecca Heald et al.   CURRENT OPINION IN CELL BIOLOGY

Giant vesicles: a powerful tool to reconstruct bacterial division assemblies in cell-like compartments

(2013) Mercedes Jiménez et al.   ENVIRONMENTAL MICROBIOLOGY

Phase transitions and size scaling of membrane-less organelles

(2013) Clifford P. Brangwynne   JOURNAL OF CELL BIOLOGY

Aqueous two-phase microdroplets with reversible phase transitions

(2013) Jonathan B. Boreyko et al.   LAB ON A CHIP

Polyelectrolyte Molecular Weight and Salt Effects on the Phase Behavior and Coacervation of Aqueous Solutions of Poly(acrylic acid) Sodium Salt and Poly(allylamine) Hydrochloride

(2013) Rungsima Chollakup et al.   MACROMOLECULES

The physical chemistry of cytoplasm and its influence on cell function: an update

(2013) Kate Luby-Phelps   MOLECULAR BIOLOGY OF THE CELL

Competition between model protocells driven by an encapsulated catalyst

(2013) Katarzyna Adamala et al.   Nature Chemistry

Enhanced transcription rates in membrane-free protocells formed by coacervation of cell lysate

(2013) E. Sokolova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ultrathin Shell Double Emulsion Templated Giant Unilamellar Lipid Vesicles with Controlled Microdomain Formation

(2013) Laura R. Arriaga et al.   Small

Biocatalyzed mineralization in an aqueous two-phase system: effect of background polymers and enzyme partitioning

(2013) David N. Cacace et al.   Journal of Materials Chemistry B

Aqueous Phase Separation as a Possible Route to Compartmentalization of Biological Molecules

(2012) Christine D. Keating   ACCOUNTS OF CHEMICAL RESEARCH

Droplet Microfluidics-A Tool for Single-Cell Analysis

(2012) Haakan N. Joensson et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Microfluidic fabrication of water-in-water (w/w) jets and emulsions

(2012) Jason Varnell et al.   Biomicrofluidics

A perspective on paper-based microfluidics: Current status and future trends

(2012) Xu Li et al.   Biomicrofluidics

Stabilization and enhanced reactivity of actinorhodin polyketide synthase minimal complex in polymer–nucleotide coacervate droplets

(2012) John Crosby et al.   CHEMICAL COMMUNICATIONS

Towards an artificial cell

(2012) Daniel A. Hammer et al.   FEBS LETTERS

Wetting-Induced Budding of Vesicles in Contact with Several Aqueous Phases

(2012) Yanhong Li et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Microfluidic methods for forming liposomes

(2012) Dirk van Swaay et al.   LAB ON A CHIP

Monodisperse w/w/w Double Emulsion Induced by Phase Separation

(2012) Yang Song et al.   LANGMUIR

Particles Trapped at the Droplet Interface in Water-in-Water Emulsions

(2012) Gireeshkumar Balakrishnan et al.   LANGMUIR

RNA catalysis through compartmentalization

(2012) Christopher A. Strulson et al.   Nature Chemistry

Fabrication of Microgel Particles with Complex Shape via Selective Polymerization of Aqueous Two-Phase Systems

(2012) Shaohua Ma et al.   Small

Lipid membranes in contact with aqueous phases of polymer solutions

(2012) Rumiana Dimova et al.   Soft Matter

Phase behaviour and complex coacervation of aqueous polypeptide solutions

(2012) Dimitrios Priftis et al.   Soft Matter

Towards a bottom-up reconstitution of bacterial cell division

(2012) Ariadna Martos et al.   TRENDS IN CELL BIOLOGY

The Structure and Function of the Eukaryotic Ribosome

(2012) D. N. Wilson et al.   Cold Spring Harbor Perspectives in Biology

Monodisperse Water-in-Water-in-Oil Emulsion Droplets

(2011) Masahiro Yasukawa et al.   CHEMPHYSCHEM

Aqueous two-phase systems for protein separation: A perspective

(2011) Juan A. Asenjo et al.   JOURNAL OF CHROMATOGRAPHY A

Complete Budding and Asymmetric Division of Primitive Model Cells To Produce Daughter Vesicles with Different Interior and Membrane Compositions

(2011) Meghan Andes-Koback et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Microfluidics with aqueous two-phase systems

(2011) Steffen Hardt et al.   LAB ON A CHIP

Surfactants in droplet-based microfluidics

(2011) Jean-Christophe Baret   LAB ON A CHIP

Denaturation of Proteins by SDS and Tetraalkylammonium Dodecyl Sulfates

(2011) Andrew Lee et al.   LANGMUIR

Peptide–nucleotide microdroplets as a step towards a membrane-free protocell model

(2011) Shogo Koga et al.   Nature Chemistry

Membrane nanotubes induced by aqueous phase separation and stabilized by spontaneous curvature

(2011) Y. Li et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes

(2011) C. P. Brangwynne et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

All-aqueous core-shell droplets produced in a microfluidic device

(2011) Iwona Ziemecka et al.   Soft Matter

Arrested coalescence in Pickering emulsions

(2011) Amar B. Pawar et al.   Soft Matter

Bacterial Microcompartments

(2010) Cheryl A. Kerfeld et al.   Annual Review of Microbiology

Giant Vesicles: Preparations and Applications

(2010) Peter Walde et al.   CHEMBIOCHEM

Monodisperse hydrogel microspheres by forced droplet formation in aqueous two-phase systems

(2010) Iwona Ziemecka et al.   LAB ON A CHIP

Polymeric Crowding Agents Improve Passive Biomacromolecule Encapsulation in Lipid Vesicles

(2010) Lisa M. Dominak et al.   LANGMUIR

Phase Behavior and Coacervation of Aqueous Poly(acrylic acid)−Poly(allylamine) Solutions

(2010) Rungsima Chollakup et al.   MACROMOLECULES

Binodal Compositions of Polyelectrolyte Complexes

(2010) Evan Spruijt et al.   MACROMOLECULES

Rapid cell extraction in aqueous two-phase microdroplet systems

(2010) Kalpana Vijayakumar et al.   Chemical Science

Microfluidic DNA amplification—A review

(2009) Yonghao Zhang et al.   ANALYTICA CHIMICA ACTA

Effect of Macromolecular Crowding on Reaction Rates: A Computational and Theoretical Study

(2009) Jun Soo Kim et al.   BIOPHYSICAL JOURNAL

Dividing cellular asymmetry: asymmetric cell division and its implications for stem cells and cancer

(2009) R. A. Neumuller et al.   GENES & DEVELOPMENT

Coupled Growth and Division of Model Protocell Membranes

(2009) Ting F. Zhu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Microcompartmentation in Artificial Cells: pH-Induced Conformational Changes Alter Protein Localization

(2009) Lisa M. Dominak et al.   LANGMUIR

Intrinsic Contact Angle of Aqueous Phases at Membranes and Vesicles

(2009) Halim Kusumaatmaja et al.   PHYSICAL REVIEW LETTERS

Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation

(2009) C. P. Brangwynne et al.   SCIENCE

Crowding Effects on Diffusion in Solutions and Cells

(2008) James A. Dix et al.   Annual Review of Biophysics

Mechanisms of Asymmetric Stem Cell Division

(2008) Juergen A. Knoblich   CELL

Positioning Lipid Membrane Domains in Giant Vesicles by Micro-organization of Aqueous Cytoplasm Mimic

(2008) Ann-Sofie Cans et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Transition from Complete to Partial Wetting within Membrane Compartments

(2008) Yanhong Li et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Biocompatible surfactants for water-in-fluorocarbon emulsions

(2008) C. Holtze et al.   LAB ON A CHIP

Macromolecular Crowding Improves Polymer Encapsulation within Giant Lipid Vesicles

(2008) Lisa M. Dominak et al.   LANGMUIR

Double Emulsion Templated Monodisperse Phospholipid Vesicles

(2008) Ho Cheung Shum et al.   LANGMUIR

Designer emulsions using microfluidics

(2008) Rhutesh K. Shah et al.   Materials Today

De Novo Formation of a Subnuclear Body

(2008) T. E. Kaiser et al.   SCIENCE

Stress granules: the Tao of RNA triage

(2008) Paul Anderson et al.   TRENDS IN BIOCHEMICAL SCIENCES

Budding and Asymmetric Protein Microcompartmentation in Giant Vesicles Containing Two Aqueous Phases

(2007) M. Scott Long et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY