Historical evolution of spheroids and organoids, and possibilities of use in life sciences and medicine

Long-term expansion, genomic stability and in vivo safety of adult human pancreas organoids

(2020) Nikitas Georgakopoulos et al.   BMC DEVELOPMENTAL BIOLOGY

Use of 3D Organoids as a Model to Study Idiopathic Form of Parkinson’s Disease

(2020) Chlebanowska et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Three-dimensional culture models mimic colon cancer heterogeneity induced by different microenvironments

(2020) Shigeto Kawai et al.   Scientific Reports

A three-dimensional intestinal tissue model reveals factors and small regulatory RNAs important for colonization with Campylobacter jejuni

(2020) Mona Alzheimer et al.   PLoS Pathogens

Organoid models in lung regeneration and cancer

(2020) Jianhai Wang et al.   CANCER LETTERS

COVID-19: organoids go viral

(2020) Hans Clevers   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Developing Organoids from Ovarian Cancer as Experimental and Preclinical Models

(2020) Nina Maenhoudt et al.   Stem Cell Reports

Human cardiac organoids for the modelling of myocardial infarction and drug cardiotoxicity

(2020) Dylan J. Richards et al.   Nature Biomedical Engineering

Midbrain Organoids: A New Tool to Investigate Parkinson’s Disease

(2020) Lisa Maria Smits et al.   Frontiers in Cell and Developmental Biology

Molecular characterization of organoids derived from pancreatic intraductal papillary mucinous neoplasms

(2020) Bo Huang et al.   JOURNAL OF PATHOLOGY

Organoid technology in female reproductive biomedicine

(2020) Heidar Heidari-Khoei et al.   Reproductive Biology and Endocrinology

A synergic approach to enhance long-term culture and manipulation of MiaPaCa-2 pancreatic cancer spheroids

(2020) Marta Cavo et al.   Scientific Reports

Exploiting CRISPR Cas9 in Three-Dimensional Stem Cell Cultures to Model Disease

(2020) Sneha Gopal et al.   Frontiers in Bioengineering and Biotechnology

Overcoming kidney organoid challenges for regenerative medicine

(2020) Thomas Geuens et al.   npj Regenerative Medicine

Promising Applications of Tumor Spheroids and Organoids for Personalized Medicine

(2020) Zarema Gilazieva et al.   Cancers

Establishment of a 3D Co-culture With MDA-MB-231 Breast Cancer Cell Line and Patient-Derived Immune Cells for Application in the Development of Immunotherapies

(2020) Diana P. Saraiva et al.   Frontiers in Oncology

Generation of lung organoids from human pluripotent stem cells in vitro

(2019) Alyssa J. Miller et al.   Nature Protocols

Systematic Comparison of Retinal Organoid Differentiation from Human Pluripotent Stem Cells Reveals Stage Specific, Cell Line, and Methodological Differences

(2019) Carla B. Mellough et al.   Stem Cells Translational Medicine

Dissecting hiPSC-CM pacemaker function in a cardiac organoid model

(2019) Mirja L. Schulze et al.   BIOMATERIALS

An organoid platform for ovarian cancer captures intra- and interpatient heterogeneity

(2019) Oded Kopper et al.   NATURE MEDICINE

Gut Organoids: Mini-Tissues in Culture to Study Intestinal Physiology and Disease

(2019) Mohammad Almeqdadi et al.   AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY

Establishment and characterization of patient‐derived organoids from a young patient with cervical clear cell carcinoma

(2019) Yoshiaki Maru et al.   CANCER SCIENCE

Impact of organ-on-a-chip technology on pharmaceutical R&D costs

(2019) Nora Franzen et al.   DRUG DISCOVERY TODAY

Multiplex quantitative analysis of stroma-mediated cancer cell invasion, matrix remodeling, and drug response in a 3D co-culture model of pancreatic tumor spheroids and stellate cells

(2019) Hyun Ju Hwang et al.   JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH

Modeling of Fibrotic Lung Disease Using 3D Organoids Derived from Human Pluripotent Stem Cells

(2019) Alexandros Strikoudis et al.   Cell Reports

In vitro expansion of endogenous human alveolar epithelial type II cells in fibroblast-free spheroid culture

(2019) Kazushige Shiraishi et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Identification of Resistance Pathways Specific to Malignancy Using Organoid Models of Pancreatic Cancer

(2019) Mariano Ponz-Sarvise et al.   CLINICAL CANCER RESEARCH

Deciphering retinal diseases through the generation of three dimensional stem cell-derived organoids: Concise Review

(2019) Ana Artero Castro et al.   STEM CELLS

Organoids of epithelial ovarian cancer as an emerging preclinical in vitro tool: a review

(2019) Sander Dumont et al.   Journal of Ovarian Research

Reversal of Surfactant Protein B Deficiency in Patient Specific Human Induced Pluripotent Stem Cell Derived Lung Organoids by Gene Therapy

(2019) Sandra Lawrynowicz Leibel et al.   Scientific Reports

Improved Retinal Organoid Differentiation by Modulating Signaling Pathways Revealed by Comparative Transcriptome Analyses with Development In Vivo

(2019) Matthew J. Brooks et al.   Stem Cell Reports

Stem-like Cancer Cells in a Dynamic 3D Culture System: A Model to Study Metastatic Cell Adhesion and Anti-cancer Drugs

(2019) Paolillo et al.   Cells

Three-dimensional cell culture systems as an in vitro platform for cancer and stem cell modeling

(2019) Nipha Chaicharoenaudomrung et al.   World Journal of Stem Cells

Three-Dimensional in Vitro Cell Culture Models in Drug Discovery and Drug Repositioning

(2018) Sigrid A. Langhans   Frontiers in Pharmacology

Modeling Host-Pathogen Interactions in the Context of the Microenvironment: 3-D Cell Culture Comes of Age

(2018) Jennifer Barrila et al.   INFECTION AND IMMUNITY

Three-Dimensional Retinal Organoids Facilitate the Investigation of Retinal Ganglion Cell Development, Organization and Neurite Outgrowth from Human Pluripotent Stem Cells

(2018) Clarisse M. Fligor et al.   Scientific Reports

Self-Renewing Trophoblast Organoids Recapitulate the Developmental Program of the Early Human Placenta

(2018) Sandra Haider et al.   Stem Cell Reports

Evaluation of variability in human kidney organoids

(2018) Belinda Phipson et al.   NATURE METHODS

Building Models of Brain Disorders with Three-Dimensional Organoids

(2018) Neal D. Amin et al.   NEURON

Importance of organoids for personalized medicine

(2018) Lukas Perkhofer et al.   Personalized Medicine

Modelling Toxoplasma gondii infection in a 3D cell culture system In Vitro: Comparison with infection in 2D cell monolayers

(2018) Jeffrey J. Danielson et al.   PLoS One

The Tissue Culture Laboratory of Dr. George Otto Gey 60 yrs ago as recalled by a former student

(2017) Charles T. Ambrose   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL

Three-dimensional cell culture models for anticancer drug screening: Worth the effort?

(2017) Eddy-Tim Verjans et al.   JOURNAL OF CELLULAR PHYSIOLOGY

A Scalable Approach for the Generation of Human Pluripotent Stem Cell-Derived Hepatic Organoids with Sensitive Hepatotoxicity Features

(2017) Malte Sgodda et al.   STEM CELLS AND DEVELOPMENT

Directed Differentiation of Human Induced Pluripotent Stem Cells into Fallopian Tube Epithelium

(2017) Nur Yucer et al.   Scientific Reports

Experimental study of tuberculosis: From animal models to complex cell systems and organoids

(2017) Kaori L. Fonseca et al.   PLoS Pathogens

Human Stromal (Mesenchymal) Stem Cells: Basic Biology and Current Clinical Use for Tissue Regeneration

(2017) Abdullah Aldahmash et al.   ANNALS OF SAUDI MEDICINE

Phenotypic Characterization of Toxic Compound Effects on Liver Spheroids Derived from iPSC Using Confocal Imaging and Three-Dimensional Image Analysis

(2016) Oksana Sirenko et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Advances in pulmonary therapy and drug development: Lung tissue engineering to lung-on-a-chip

(2016) Ali Doryab et al.   BIOTECHNOLOGY ADVANCES

Modeling Development and Disease with Organoids

(2016) Hans Clevers   CELL

Organoids: A historical perspective of thinking in three dimensions

(2016) Marina Simian et al.   JOURNAL OF CELL BIOLOGY

Engineered human pluripotent-stem-cell-derived intestinal tissues with a functional enteric nervous system

(2016) Michael J Workman et al.   NATURE MEDICINE

Stem cell-derived organoids to model gastrointestinal facets of cystic fibrosis

(2016) Meike Hohwieler et al.   United European Gastroenterology Journal

Mini-Gut Organoids: Reconstitution of the Stem Cell Niche

(2015) Shoichi Date et al.   Annual Review of Cell and Developmental Biology

Modeling mouse and human development using organoid cultures

(2015) M. Huch et al.   DEVELOPMENT

Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation

(2015) Fotios Sampaziotis et al.   NATURE BIOTECHNOLOGY

Ductal pancreatic cancer modeling and drug screening using human pluripotent stem cell– and patient-derived tumor organoids

(2015) Ling Huang et al.   NATURE MEDICINE

Dppa3 expression is critical for generation of fully reprogrammed iPS cells and maintenance of Dlk1-Dio3 imprinting

(2015) Xingbo Xu et al.   Nature Communications

Three-Dimensional Cell Culture Systems and Their Applications in Drug Discovery and Cell-Based Biosensors

(2014) Rasheena Edmondson et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Microscale screening systems for 3D cellular microenvironments: platforms, advances, and challenges

(2014) Sara I. Montanez-Sauri et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Microfluidic organs-on-chips

(2014) Sangeeta N Bhatia et al.   NATURE BIOTECHNOLOGY

3D bioprinting of tissues and organs

(2014) Sean V Murphy et al.   NATURE BIOTECHNOLOGY

Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells

(2014) Alireza Rezania et al.   NATURE BIOTECHNOLOGY

Cerebral organoids model human brain development and microcephaly

(2013) Madeline A. Lancaster et al.   NATURE

Three-dimensional cell culturing by magnetic levitation

(2013) William L Haisler et al.   Nature Protocols

Phase II and Phase III attrition rates 2011–2012

(2013) John Arrowsmith et al.   NATURE REVIEWS DRUG DISCOVERY

Identification of Lgr5-Independent Spheroid-Generating Progenitors of the Mouse Fetal Intestinal Epithelium

(2013) Roxana C. Mustata et al.   Cell Reports

TBX3 Directs Cell-Fate Decision toward Mesendoderm

(2013) Clair E. Weidgang et al.   Stem Cell Reports

The potential of human fetal mesenchymal stem cells for off-the-shelf bone tissue engineering application

(2012) Zhi-Yong Zhang et al.   BIOMATERIALS

How to Remake a Fibroblast into a Neural Stem Cell

(2012) Qiao Zhou et al.   Cell Stem Cell

Direct Reprogramming of Fibroblasts into Neural Stem Cells by Defined Factors

(2012) Dong Wook Han et al.   Cell Stem Cell

Self-Formation of Optic Cups and Storable Stratified Neural Retina from Human ESCs

(2012) Tokushige Nakano et al.   Cell Stem Cell

Synthetic 3D multicellular systems for drug development

(2012) Markus Rimann et al.   CURRENT OPINION IN BIOTECHNOLOGY

Three-dimensional cell culture: the missing link in drug discovery

(2012) Susan Breslin et al.   DRUG DISCOVERY TODAY

The Pathology of Mycobacterium tuberculosis Infection

(2012) K. Sakamoto   VETERINARY PATHOLOGY

Self-organizing optic-cup morphogenesis in three-dimensional culture

(2011) Mototsugu Eiraku et al.   NATURE

Optic Vesicle-like Structures Derived from Human Pluripotent Stem Cells Facilitate a Customized Approach to Retinal Disease Treatment

(2011) Jason S. Meyer et al.   STEM CELLS

Preparing for the First Breath: Genetic and Cellular Mechanisms in Lung Development

(2010) Edward E. Morrisey et al.   DEVELOPMENTAL CELL

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

(2009) Toshiro Sato et al.   NATURE

Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche

(2009) Akifumi Ootani et al.   NATURE MEDICINE

An Approach for Formation of Vascularized Liver Tissue by Endothelial Cell–Covered Hepatocyte Spheroid Integration

(2009) Masakazu Inamori et al.   TISSUE ENGINEERING PART A