Roadmap to Building a Cell: An Evolutionary Approach

The long journey towards standards for engineering biosystems

(2020) Jacob Beal et al.   EMBO REPORTS

Definitions of life as epistemic tools that reflect and foster the advance of biological knowledge

(2020) Alba Amilburu et al.   SYNTHESE

Gene Expression Inside Liposomes: From Early Studies to Current Protocols

(2019) Pasquale Stano   CHEMISTRY-A EUROPEAN JOURNAL

Evolutionary Innovations and Where to Find Them: Routes to Open-Ended Evolution in Natural and Artificial Systems

(2019) Tim Taylor   ARTIFICIAL LIFE

Cell Fuelling and Metabolic Energy Conservation in Synthetic Cells

(2019) Hendrik R. Sikkema et al.   CHEMBIOCHEM

Learning unsupervised feature representations for single cell microscopy images with paired cell inpainting

(2019) Alex X. Lu et al.   PLoS Computational Biology

Innovation by Evolution: Bringing New Chemistry to Life (Nobel Lecture)

(2019) Frances H. Arnold   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor

(2019) Haiqi Chen et al.   NATURE BIOTECHNOLOGY

CRISPR base editors: genome editing without double-stranded breaks

(2018) Ayman Eid et al.   BIOCHEMICAL JOURNAL

Self-replication of DNA by its encoded proteins in liposome-based synthetic cells

(2018) Pauline van Nies et al.   Nature Communications

Transient AID expression for in situ mutagenesis with improved cellular fitness

(2018) Talal Salem Al-Qaisi et al.   Scientific Reports

Intelligent Image-Activated Cell Sorting

(2018) Nao Nitta et al.   CELL

Compartmentalized partnered replication for the directed evolution of genetic parts and circuits

(2017) Zhanar Abil et al.   Nature Protocols

Cell-free protein synthesis in micro compartments: building a minimal cell from biobricks

(2017) Haiyang Jia et al.   New Biotechnology

Conceptualizing the origin of life in terms of evolution

(2017) N. Takeuchi et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES

Chemical roots of biological evolution: the origins of life as a process of development of autonomous functional systems

(2017) Kepa Ruiz-Mirazo et al.   Open Biology

Artificial cells: from basic science to applications

(2016) Can Xu et al.   Materials Today

Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells

(2016) Gaelen T Hess et al.   NATURE METHODS

Defining and simulating open-ended novelty: requirements, guidelines, and challenges

(2016) Wolfgang Banzhaf et al.   THEORY IN BIOSCIENCES

Methods for the directed evolution of proteins

(2015) Michael S. Packer et al.   NATURE REVIEWS GENETICS

Integration of biological parts toward the synthesis of a minimal cell

(2014) Filippo Caschera et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Functional optimization of gene clusters by combinatorial design and assembly

(2014) Michael J Smanski et al.   NATURE BIOTECHNOLOGY

Liposome display for in vitro selection and evolution of membrane proteins

(2014) Satoshi Fujii et al.   Nature Protocols

Image-Based Single-Cell Sorting via Dual-Photopolymerized Microwell Arrays

(2013) Tao Sun et al.   ANALYTICAL CHEMISTRY

The Evolutionary Origin of Biological Function and Complexity

(2013) Addy Pross   JOURNAL OF MOLECULAR EVOLUTION

Development of an artificial cell, from self-organization to computation and self-reproduction

(2011) Vincent Noireaux et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Autonomy in evolution: from minimal to complex life

(2011) Kepa Ruiz-Mirazo et al.   SYNTHESE

Build life to understand it

(2010) Michael Elowitz et al.   NATURE

Is it Useful to Have a Clear-cut Definition of Life? On the Use of Fuzzy Logic in Prebiotic Chemistry

(2010) Gilles Bruylants et al.   ORIGINS OF LIFE AND EVOLUTION OF BIOSPHERES

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS