Compartmentalized partnered replication for the directed evolution of genetic parts and circuits

Predictable tuning of protein expression in bacteria

(2016) Mads T Bonde et al.   NATURE METHODS

Synthetic evolutionary origin of a proofreading reverse transcriptase

(2016) J. W. Ellefson et al.   SCIENCE

Evolving Orthogonal Suppressor tRNAs To Incorporate Modified Amino Acids

(2016) Andre C. Maranhao et al.   ACS Synthetic Biology

Enzyme engineering in biomimetic compartments

(2015) Pierre-Yves Colin et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Methods for the directed evolution of proteins

(2015) Michael S. Packer et al.   NATURE REVIEWS GENETICS

Directed Evolution of a Panel of Orthogonal T7 RNA Polymerase Variants for in Vivo or in Vitro Synthetic Circuitry

(2014) Adam J. Meyer et al.   ACS Synthetic Biology

Polishing the craft of genetic diversity creation in directed evolution

(2013) Kang Lan Tee et al.   BIOTECHNOLOGY ADVANCES

Reporter-based screening and selection of enzymes

(2013) Teunke van Rossum et al.   FEBS Journal

Directed evolution of genetic parts and circuits by compartmentalized partnered replication

(2013) Jared W Ellefson et al.   NATURE BIOTECHNOLOGY

Single-cell analysis and sorting using droplet-based microfluidics

(2013) Linas Mazutis et al.   Nature Protocols

Understanding, predicting and manipulating the genotypic evolution of antibiotic resistance

(2013) Adam C. Palmer et al.   NATURE REVIEWS GENETICS

A streamlined protocol for emulsion polymerase chain reaction and subsequent purification

(2010) Tatjana Schütze et al.   ANALYTICAL BIOCHEMISTRY

High-Throughput Metabolic Engineering: Advances in Small-Molecule Screening and Selection

(2010) Jeffrey A. Dietrich et al.   Annual Review of Biochemistry

CyDNA: Synthesis and Replication of Highly Cy-Dye Substituted DNA by an Evolved Polymerase

(2010) Nicola Ramsay et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Design, construction and characterization of a set of insulated bacterial promoters

(2010) J. H. Davis et al.   NUCLEIC ACIDS RESEARCH

Ultrahigh-Throughput FACS-Based Screening for Directed Enzyme Evolution

(2009) Guangyu Yang et al.   CHEMBIOCHEM

Evolving a Polymerase for Hydrophobic Base Analogues

(2009) David Loakes et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Exploring protein fitness landscapes by directed evolution

(2009) Philip A. Romero et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Measuring the activity of BioBrick promoters using an in vivo reference standard

(2009) Jason R Kelly et al.   Journal of Biological Engineering

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

(2008) Carola Engler et al.   PLoS One