Applications of CRISPR–Cas in agriculture and plant biotechnology

Base-Editing-Mediated Artificial Evolution of OsALS1 In Planta to Develop Novel Herbicide-Tolerant Rice Germplasms

(2020) Yongjie Kuang et al.   Molecular Plant

Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors

(2020) Chao Li et al.   NATURE BIOTECHNOLOGY

A CRISPR‐Cas9‐mediated domain‐specific base‐editing screen enables functional assessment of ACCase variants in rice

(2020) Xiaoshuang Liu et al.   PLANT BIOTECHNOLOGY JOURNAL

A variety of changes, including CRISPR/Cas9 mediated deletions, in CENH3 lead to haploid induction on outcrossing

(2020) Sundaram Kuppu et al.   PLANT BIOTECHNOLOGY JOURNAL

Production of novel beneficial alleles of a rice yield‐related QTL by CRISPR/Cas9

(2020) Yongtao Cui et al.   PLANT BIOTECHNOLOGY JOURNAL

High-Throughput CRISPR/Cas9 Mutagenesis Streamlines Trait Gene Identification in Maize

(2020) Haijun Liu et al.   PLANT CELL

A biotechnology‐based male‐sterility system for hybrid seed production in tomato

(2020) Minmin Du et al.   PLANT JOURNAL

Rational Improvement of Rice Yield and Cold Tolerance by Editing the Three Genes OsPIN5b, GS3, and OsMYB30 With the CRISPR–Cas9 System

(2020) Yafei Zeng et al.   Frontiers in Plant Science

Overcoming bottlenecks in plant gene editing

(2020) Paul AP Atkins et al.   CURRENT OPINION IN PLANT BIOLOGY

Versatile Nucleotides Substitution in Plant Using an Improved Prime Editing System

(2020) Wen Xu et al.   Molecular Plant

Directed evolution of adenine base editors with increased activity and therapeutic application

(2020) Nicole M. Gaudelli et al.   NATURE BIOTECHNOLOGY

Superior field performance of waxy corn engineered using CRISPR–Cas9

(2020) Huirong Gao et al.   NATURE BIOTECHNOLOGY

Prime genome editing in rice and wheat

(2020) Qiupeng Lin et al.   NATURE BIOTECHNOLOGY

Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity

(2020) Michelle F. Richter et al.   NATURE BIOTECHNOLOGY

Generation of homozygous canker‐resistant citrus in the T0 generation using CRISPR‐SpCas9p

(2020) Hongge Jia et al.   PLANT BIOTECHNOLOGY JOURNAL

Creating novel Wx alleles with fine‐tuned amylose levels and improved grain quality in rice by promoter editing using CRISPR/Cas9 system

(2020) Lichun Huang et al.   PLANT BIOTECHNOLOGY JOURNAL

Site‐directed mutagenesis in bread and durum wheat via pollination by cas9 /guide RNA‐transgenic maize used as haploidy inducer

(2020) Nagaveni Budhagatapalli et al.   PLANT BIOTECHNOLOGY JOURNAL

ENOregulates tomato fruit size through the floral meristem development network

(2020) Fernando J. Yuste-Lisbona et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Targeted delivery of nanomaterials with chemical cargoes in plants enabled by a biorecognition motif

(2020) Israel Santana et al.   Nature Communications

Fed Macrophages Hit the Liver’s Sweet Spot with IL-10

(2020) André Sulen et al.   MOLECULAR CELL

Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC–Cas9

(2020) Shengxing Wang et al.   NATURE BIOTECHNOLOGY

Optogenetic control of gene expression in plants in the presence of ambient white light

(2020) Rocio Ochoa-Fernandez et al.   NATURE METHODS

Quantitative regulation of Waxy expression by CRISPR/Cas9‐based promoter and 5'UTR‐intron editing improves grain quality in rice

(2020) Dongchang Zeng et al.   PLANT BIOTECHNOLOGY JOURNAL

Fine‐tuning the amylose content of rice by precise base editing of the Wx gene

(2020) Yang Xu et al.   PLANT BIOTECHNOLOGY JOURNAL

Developing a novel artificial rice germplasm for dinitroaniline herbicide resistance by base editing of OsTubA2

(2020) Lang Liu et al.   PLANT BIOTECHNOLOGY JOURNAL

Shortening the sgRNA-DNA interface enables SpCas9 and eSpCas9(1.1) to nick the target DNA strand

(2020) Rong Fan et al.   Science China-Life Sciences

Roadmap for Accelerated Domestication of an Emerging Perennial Grain Crop

(2020) Lee DeHaan et al.   TRENDS IN PLANT SCIENCE

Clonal seeds from hybrid rice by simultaneous genome engineering of meiosis and fertilization genes

(2019) Chun Wang et al.   NATURE BIOTECHNOLOGY

CRISPR/Cas9-mediated multiple gene editing in Brassica oleracea var. capitata using the endogenous tRNA-processing system

(2019) Cunfa Ma et al.   Horticulture Research

Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems

(2019) Ashot Papikian et al.   Nature Communications

DNA nanostructures coordinate gene silencing in mature plants

(2019) Huan Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CRISPR/Cas-mediated gene targeting in plants: finally a turn for the better for homologous recombination

(2019) Teng-Kuei Huang et al.   PLANT CELL REPORTS

CRISPR/Cas9-mediated knockout of Ms1 enables the rapid generation of male-sterile hexaploid wheat lines for use in hybrid seed production

(2019) Anzu Okada et al.   PLANT BIOTECHNOLOGY JOURNAL

Development of an Agrobacterium -delivered CRISPR/Cas9 system for wheat genome editing

(2019) Zhengzhi Zhang et al.   PLANT BIOTECHNOLOGY JOURNAL

Chloroplast-selective gene delivery and expression in planta using chitosan-complexed single-walled carbon nanotube carriers

(2019) Seon-Yeong Kwak et al.   Nature Nanotechnology

High aspect ratio nanomaterials enable delivery of functional genetic material without DNA integration in mature plants

(2019) Gozde S. Demirer et al.   Nature Nanotechnology

One-step genome editing of elite crop germplasm during haploid induction

(2019) Timothy Kelliher et al.   NATURE BIOTECHNOLOGY

Development of a Haploid-Inducer Mediated Genome Editing System for Accelerating Maize Breeding

(2019) Baobao Wang et al.   Molecular Plant

CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture

(2019) Kunling Chen et al.   Annual Review of Plant Biology

Reversible Disruption of Specific Transcription Factor-DNA Interactions Using CRISPR/Cas9

(2019) S. Ali Shariati et al.   MOLECULAR CELL

De Novo Domestication: An Alternative Route toward New Crops for the Future

(2019) Alisdair R. Fernie et al.   Molecular Plant

Rescue of a plant cytorhabdovirus as versatile expression platforms for planthopper and cereal genomic studies

(2019) Qiang Gao et al.   NEW PHYTOLOGIST

Functional Analysis of M-Locus Protein Kinase Revealed a Novel Regulatory Mechanism of Self-Incompatibility in Brassica napus L.

(2019) Fang Chen et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

A barley stripe mosaic virus‐based guide RNA delivery system for targeted mutagenesis in wheat and maize

(2019) Jiacheng Hu et al.   MOLECULAR PLANT PATHOLOGY

Extension of the in vivo haploid induction system from diploid maize to hexaploid wheat

(2019) Chenxu Liu et al.   PLANT BIOTECHNOLOGY JOURNAL

Engineering Broad-Spectrum Bacterial Blight Resistance by Simultaneously Disrupting Variable TALE-binding Elements of Multiple Susceptibility Genes in Rice

(2019) Zhengyin Xu et al.   Molecular Plant

Multiplexed genome engineering by Cas12a and CRISPR arrays encoded on single transcripts

(2019) Carlo C. Campa et al.   NATURE METHODS

Simplified adenine base editors improve adenine base editing efficiency in rice

(2019) Kai Hua et al.   PLANT BIOTECHNOLOGY JOURNAL

Generation of a multiplex mutagenesis population via pooled CRISPR ‐Cas9 in soya bean

(2019) Mengyan Bai et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR-TSKO: A Technique for Efficient Mutagenesis in Specific Cell Types, Tissues, or Organs in Arabidopsis

(2019) Ward Decaestecker et al.   PLANT CELL

A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize

(2019) Qiang Zhang et al.   PLANT PHYSIOLOGY

Mutational Analysis of OsPLDα1 Reveals Its Involvement in Phytic Acid Biosynthesis in Rice Grains

(2019) Muhammad Saad Shoaib Khan et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

Broad-spectrum resistance to bacterial blight in rice using genome editing

(2019) Ricardo Oliva et al.   NATURE BIOTECHNOLOGY

Plant gene editing through de novo induction of meristems

(2019) Michael F. Maher et al.   NATURE BIOTECHNOLOGY

A cytokinin-activation enzyme-like gene improves grain yield under various field conditions in rice

(2019) Changgui Wang et al.   PLANT MOLECULAR BIOLOGY

Rapid genotype “independent” Zea mays L. (maize) transformation via direct somatic embryogenesis

(2018) Keith Lowe et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

Improved Base Editor for Efficiently Inducing Genetic Variations in Rice with CRISPR/Cas9-Guided Hyperactive hAID Mutant

(2018) Bin Ren et al.   Molecular Plant

Robust Transcriptional Activation in Plants Using Multiplexed CRISPR-Act2.0 and mTALE-Act Systems

(2018) Levi G. Lowder et al.   Molecular Plant

Engineering Introns to Express RNA Guides for Cas9- and Cpf1-Mediated Multiplex Genome Editing

(2018) Dan Ding et al.   Molecular Plant

With a free pass, CRISPR-edited plants reach market in record time

(2018) Emily Waltz   NATURE BIOTECHNOLOGY

Blocking amino acid transporter OsAAP3 improves grain yield by promoting outgrowth buds and increasing tiller number in rice

(2018) Kai Lu et al.   PLANT BIOTECHNOLOGY JOURNAL

Establishing RNA virus resistance in plants by harnessing CRISPR immune system

(2018) Tong Zhang et al.   PLANT BIOTECHNOLOGY JOURNAL

Allele exchange at the EPSPS locus confers glyphosate tolerance in cassava

(2018) Aaron W. Hummel et al.   PLANT BIOTECHNOLOGY JOURNAL

Analysis of the functions of TaGW2 homoeologs in wheat grain weight and protein content traits

(2018) Yi Zhang et al.   PLANT JOURNAL

A farnesyl pyrophosphate synthase gene expressed in pollen functions in S -RNase-independent unilateral incompatibility

(2018) Xiaoqiong Qin et al.   PLANT JOURNAL

Concurrent modifications in the three homeologs of Ms45 gene with CRISPR-Cas9 lead to rapid generation of male sterile bread wheat (Triticum aestivum L.)

(2018) Manjit Singh et al.   PLANT MOLECULAR BIOLOGY

Targeted DNA demethylation of theArabidopsisgenome using the human TET1 catalytic domain

(2018) Javier Gallego-Bartolomé et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Pea early-browning virus -mediated genome editing via the CRISPR/Cas9 system in Nicotiana benthamiana and Arabidopsis

(2018) Zahir Ali et al.   VIRUS RESEARCH

True gene-targeting events by CRISPR/Cas-induced DSB repair of the PPO locus with an ectopically integrated repair template

(2018) Sylvia de Pater et al.   Scientific Reports

Lycopene Is Enriched in Tomato Fruit by CRISPR/Cas9-Mediated Multiplex Genome Editing

(2018) Xindi Li et al.   Frontiers in Plant Science

Genome editing of upstream open reading frames enables translational control in plants

(2018) Huawei Zhang et al.   NATURE BIOTECHNOLOGY

Expanding the base editing scope in rice by using Cas9 variants

(2018) Kai Hua et al.   PLANT BIOTECHNOLOGY JOURNAL

Multiplex QTL editing of grain-related genes improves yield in elite rice varieties

(2018) Jianping Zhou et al.   PLANT CELL REPORTS

Engineered CRISPR-Cas9 nuclease with expanded targeting space

(2018) Hiroshi Nishimasu et al.   SCIENCE

Manipulating mRNA splicing by base editing in plants

(2018) Chenxiao Xue et al.   Science China-Life Sciences

A male-expressed rice embryogenic trigger redirected for asexual propagation through seeds

(2018) Imtiyaz Khanday et al.   NATURE

Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A

(2018) Yuan Zong et al.   NATURE BIOTECHNOLOGY

Domestication of wild tomato is accelerated by genome editing

(2018) Tingdong Li et al.   NATURE BIOTECHNOLOGY

De novo domestication of wild tomato using genome editing

(2018) Agustin Zsögön et al.   NATURE BIOTECHNOLOGY

Gene disruption through base editing-induced mRNA mis-splicing in plants

(2018) Zhenxiang Li et al.   NEW PHYTOLOGIST

Development of Beet necrotic yellow vein virus -based vectors for multiple-gene expression and guide RNA delivery in plant genome editing

(2018) Ning Jiang et al.   PLANT BIOTECHNOLOGY JOURNAL

Generation of thermosensitive male-sterile maize by targeted knockout of the ZmTMS5 gene

(2017) Jun Li et al.   Journal of Genetics and Genomics

A 4-bp Insertion at ZmPLA1 Encoding a Putative Phospholipase A Generates Haploid Induction in Maize

(2017) Chenxu Liu et al.   Molecular Plant

Interspecific Hybrid Sterility in Rice Is Mediated by  OgTPR1 at the S1 Locus Encoding a Peptidase-like Protein

(2017) Yongyao Xie et al.   Molecular Plant

Multiplex Gene Editing in Rice Using the CRISPR-Cpf1 System

(2017) Mugui Wang et al.   Molecular Plant

Genome-wide Targeted Mutagenesis in Rice Using the CRISPR/Cas9 System

(2017) Yuming Lu et al.   Molecular Plant

Construction of a Genome-Wide Mutant Library in Rice Using CRISPR/Cas9

(2017) Xiangbing Meng et al.   Molecular Plant

RNA targeting with CRISPR–Cas13

(2017) Omar O. Abudayyeh et al.   NATURE

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

(2017) Nicole M. Gaudelli et al.   NATURE

Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion

(2017) Yuan Zong et al.   NATURE BIOTECHNOLOGY

Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion

(2017) Zenpei Shimatani et al.   NATURE BIOTECHNOLOGY

In Planta Processing of the SpCas9–gRNA Complex

(2017) Masafumi Mikami et al.   PLANT AND CELL PHYSIOLOGY

Multiplexed CRISPR/Cas9-mediated metabolic engineering of γ-aminobutyric acid levels in Solanum lycopersicum

(2017) Rui Li et al.   PLANT BIOTECHNOLOGY JOURNAL

Engineering canker-resistant plants through CRISPR/Cas9-targeted editing of the susceptibility gene CsLOB1 promoter in citrus

(2017) Aihong Peng et al.   PLANT BIOTECHNOLOGY JOURNAL

Low-gluten, nontransgenic wheat engineered with CRISPR/Cas9

(2017) Susana Sánchez-León et al.   PLANT BIOTECHNOLOGY JOURNAL

A multi-purpose toolkit to enable advanced genome engineering in plants

(2017) Tomas Cermak et al.   PLANT CELL

Simultaneous modification of three homoeologs of TaEDR1 by genome editing enhances powdery mildew resistance in wheat

(2017) Yunwei Zhang et al.   PLANT JOURNAL

Multiplexed Gene Editing and Protein Overexpression Using a Tobacco mosaic virus Viral Vector

(2017) Will B. Cody et al.   PLANT PHYSIOLOGY

Generation of a Collection of Mutant Tomato Lines Using Pooled CRISPR Libraries

(2017) Thomas B. Jacobs et al.   PLANT PHYSIOLOGY

Current and future editing reagent delivery systems for plant genome editing

(2017) Yidong Ran et al.   Science China-Life Sciences

Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping

(2017) Stefanie L. Morgan et al.   Nature Communications

Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion

(2017) Vladimir Nekrasov et al.   Scientific Reports

Engineering Herbicide-Resistant Rice Plants through CRISPR/Cas9-Mediated Homologous Recombination of Acetolactate Synthase

(2016) Yongwei Sun et al.   Molecular Plant

Development of broad virus resistance in non-transgenic cucumber using CRISPR/Cas9 technology

(2016) Jeyabharathy Chandrasekaran et al.   MOLECULAR PLANT PATHOLOGY

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

(2016) Keith Lowe et al.   PLANT CELL

Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds

(2015) Jesse G. Zalatan et al.   CELL

Efficient Virus-Mediated Genome Editing in Plants Using the CRISPR/Cas9 System

(2015) Zahir Ali et al.   Molecular Plant

DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins

(2015) Je Wook Woo et al.   NATURE BIOTECHNOLOGY

Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease

(2015) James E Dahlman et al.   NATURE BIOTECHNOLOGY

Characteristics of Plant Essential Genes Allow for within- and between-Species Prediction of Lethal Mutant Phenotypes

(2015) John P. Lloyd et al.   PLANT CELL

A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation

(2015) Levi G. Lowder et al.   PLANT PHYSIOLOGY

Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system

(2015) Kabin Xie et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew

(2014) Yanpeng Wang et al.   NATURE BIOTECHNOLOGY

New approaches to facilitate rapid domestication of a wild plant to an oilseed crop: Example pennycress (Thlaspi arvense L.)

(2014) John C. Sedbrook et al.   PLANT SCIENCE

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

Self-processing of ribozyme-flanked RNAs into guide RNAsin vitroandin vivofor CRISPR-mediated genome editing

(2013) Yangbin Gao et al.   Journal of Integrative Plant Biology

Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9

(2013) Jian-Feng Li et al.   NATURE BIOTECHNOLOGY

Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease

(2013) Vladimir Nekrasov et al.   NATURE BIOTECHNOLOGY

Targeted genome modification of crop plants using a CRISPR-Cas system

(2013) Qiwei Shan et al.   NATURE BIOTECHNOLOGY

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Global food demand and the sustainable intensification of agriculture

(2011) D. Tilman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Evolution in Action: Plants Resistant to Herbicides

(2010) Stephen B. Powles et al.   Annual Review of Plant Biology

Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

(2010) M. Christian et al.   GENETICS