Making and Breaking Peptide Bonds: Protein Engineering Using Sortase

A Biosynthetic Strategy for Re-engineering theStaphylococcus aureusCell Wall with Non-native Small Molecules

(2010) James W. Nelson et al.   ACS Chemical Biology

The Engineering of an Orally Active Conotoxin for the Treatment of Neuropathic Pain

(2010) Richard J. Clark et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Highly Oriented Recombinant Glycosyltransferases: Site-Specific Immobilization of Unstable Membrane Proteins by UsingStaphylococcus aureusSortase A

(2010) Takaomi Ito et al.   BIOCHEMISTRY

Enzyme-Mediated Site-Specific Antibody−Protein Modification Using a ZZ Domain as a Linker

(2010) Takayuki Sakamoto et al.   BIOCONJUGATE CHEMISTRY

The use of sortase-mediated ligation for the immobilisation of bacterial adhesins onto fluorescence-labelled microspheres: a novel approach to analyse bacterial adhesion to host cells

(2010) Si Wu et al.   BIOTECHNOLOGY LETTERS

Biotinylated-sortase self-cleavage purification (BISOP) method for cell-free produced proteins

(2010) Satoko Matsunaga et al.   BMC BIOTECHNOLOGY

Biological Applications of Protein Splicing

(2010) Miquel Vila-Perelló et al.   CELL

Chemoenzymatic synthesis of glycosylphosphatidylinositol-anchored glycopeptides

(2010) Zhimeng Wu et al.   CHEMICAL COMMUNICATIONS

The Sortase A Enzyme That Attaches Proteins to the Cell Wall ofBacillus anthracisContains an Unusual Active Site Architecture

(2010) Ethan M. Weiner et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Observing selected domains in multi-domain proteins via sortase-mediated ligation and NMR spectroscopy

(2010) Mary Anne Refaei et al.   JOURNAL OF BIOMOLECULAR NMR

Sortase A-Catalyzed Transpeptidation of Glycosylphosphatidylinositol Derivatives for Chemoenzymatic Synthesis of GPI-Anchored Proteins

(2010) Zhimeng Wu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Functional Neoglycopeptides: Synthesis and Characterization of a New Class of MUC1 Glycoprotein Models Having Core 2-BasedO-Glycan and Complex-TypeN-Glycan Chains

(2009) Takahiko Matsushita et al.   BIOCHEMISTRY

Sortase Activity Is Controlled by a Flexible Lid in the Pilus Biogenesis Mechanism of Gram-Positive Pathogens,

(2009) Clothilde Manzano et al.   BIOCHEMISTRY

Expansion of the sortase-mediated labeling method for site-specific N-terminal labeling of cell surface proteins on living cells

(2009) Teruyasu Yamamoto et al.   CHEMICAL COMMUNICATIONS

Amino Acid Residues Critical for Endoplasmic Reticulum Export and Trafficking of Platelet-activating Factor Receptor

(2009) Nobuaki Hirota et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A Straight Path to Circular Proteins

(2009) John M. Antos et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Crystal Structure ofStreptococcus pyogenesSortase A

(2009) Paul R. Race et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The Structure of theStaphylococcus aureusSortase-Substrate Complex Reveals How the Universally Conserved LPXTG Sorting Signal Is Recognized

(2009) Nuttee Suree et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Attachment of an NMR-invisible solubility enhancement tag using a sortase-mediated protein ligation method

(2009) Yoshihiro Kobashigawa et al.   JOURNAL OF BIOMOLECULAR NMR

Two Crystal Structures of Pneumococcal Pilus Sortase C Provide Novel Insights into Catalysis and Substrate Specificity

(2009) Fabrice Neiers et al.   JOURNAL OF MOLECULAR BIOLOGY

Sortase-Catalyzed Peptide−Glycosylphosphatidylinositol Analogue Ligation

(2009) Xueqing Guo et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Site-Specific N- and C-Terminal Labeling of a Single Polypeptide Using Sortases of Different Specificity

(2009) John M. Antos et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Production of recombinant proteins in Escherichia coli using an N-terminal tag derived from sortase

(2009) Jill Caswell et al.   PROTEIN EXPRESSION AND PURIFICATION

Synthesis of Protein Mimics with Nonlinear Backbone Topology by a Combined Recombinant, Enzymatic, and Chemical Synthesis Strategy

(2008) Stephan Pritz et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Immobilization of proteins to biacore sensor chips using Staphylococcus aureus sortase A

(2008) Fiona Clow et al.   BIOTECHNOLOGY LETTERS

Pili in Gram-negative and Gram-positive bacteria — structure, assembly and their role in disease

(2008) T. Proft et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Site-Specific Protein Modification on Living Cells Catalyzed by Sortase

(2008) Tsutomu Tanaka et al.   CHEMBIOCHEM

Substrate Filtering by the Active Site Crossover Loop in UCHL3 Revealed by Sortagging and Gain-of-function Mutations

(2008) Maximilian W. Popp et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Cell Wall Anchor Structure of BcpA Pili inBacillus anthracis

(2008) Jonathan M. Budzik et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Mutagenesis Studies of Substrate Recognition and Catalysis in the Sortase A Transpeptidase fromStaphylococcus aureus

(2008) Matthew L. Bentley et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Peptide−Sugar Ligation Catalyzed by Transpeptidase Sortase:  A Facile Approach to Neoglycoconjugate Synthesis

(2008) Sharmishtha Samantaray et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Lipid Modification of Proteins through Sortase-Catalyzed Transpeptidation

(2008) John M. Antos et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Sortase as a Target of Anti-Infective Therapy

(2008) A. W. Maresso et al.   PHARMACOLOGICAL REVIEWS

Amide bonds assemble pili on the surface of bacilli

(2008) J. M. Budzik et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The molecular switch that activates the cell wall anchoring step of pilus assembly in gram-positive bacteria

(2008) A. Mandlik et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Sortase-Mediated Pilus Fiber Biogenesis in Streptococcus pneumoniae

(2008) Clothilde Manzano et al.   STRUCTURE

Pili in Gram-positive bacteria: assembly, involvement in colonization and biofilm development

(2008) Anjali Mandlik et al.   TRENDS IN MICROBIOLOGY