Modelling human embryogenesis: embryo-like structures spark ethical and policy debate

Single-cell and spatial transcriptomics reveal somitogenesis in gastruloids

(2020) Susanne C. van den Brink et al.   NATURE

Toward Guidelines for Research on Human Embryo Models Formed from Stem Cells

(2020) Insoo Hyun et al.   Stem Cell Reports

Implantation initiation of self-assembled embryo-like structures generated using three types of mouse blastocyst-derived stem cells

(2019) Shaopeng Zhang et al.   Nature Communications

A 3D model of a human epiblast reveals BMP4-driven symmetry breaking

(2019) Mijo Simunovic et al.   NATURE CELL BIOLOGY

The moral status of cerebral organoids

(2019) Sorin Hostiuc et al.   Regenerative Therapy

Mapping cell migrations and fates in a gastruloid model to the human primitive streak

(2019) Iain Martyn et al.   DEVELOPMENT

Human embryo implantation modelled in microfluidic channels

(2019) Amander T. Clark   NATURE

Controlled modelling of human epiblast and amnion development using stem cells

(2019) Yi Zheng et al.   NATURE

Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells

(2019) Cody Kime et al.   Stem Cell Reports

Generation of Blastocyst-like Structures from Mouse Embryonic and Adult Cell Cultures

(2019) Ronghui Li et al.   CELL

Synthetic developmental biology: build and control multicellular systems

(2019) Mo R Ebrahimkhani et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Naïve human pluripotent stem cells respond to Wnt, Nodal and LIF signalling to produce expandable naïve extra-embryonic endoderm

(2019) Madeleine Linneberg-Agerholm et al.   DEVELOPMENT

In vitro culture of cynomolgus monkey embryos beyond early gastrulation

(2019) Huaixiao Ma et al.   SCIENCE

Dissecting primate early post-implantation development using long-term in vitro embryo culture

(2019) Yuyu Niu et al.   SCIENCE

High-throughput micropatterning platform reveals Nodal-dependent bisection of peri-gastrulation–associated versus preneurulation-associated fate patterning

(2019) Mukul Tewary et al.   PLOS BIOLOGY

First PGT-A using human in vivo blastocysts recovered by uterine lavage: comparison with matched IVF embryo controls†

(2019) Santiago Munné et al.   HUMAN REPRODUCTION

Derivation of Human Trophoblast Stem Cells

(2018) Hiroaki Okae et al.   Cell Stem Cell

Of mice and human embryos: is there an ethically preferred order of preclinical research on new assisted reproductive technologies?

(2018) Verna Jans et al.   HUMAN REPRODUCTION

Cerebral organoids: ethical issues and consciousness assessment

(2018) Andrea Lavazza et al.   JOURNAL OF MEDICAL ETHICS

The ethics of experimenting with human brain tissue

(2018) Nita A. Farahany et al.   NATURE

Blastocyst-like structures generated solely from stem cells

(2018) Nicolas C. Rivron et al.   NATURE

Self-assembly of embryonic and two extra-embryonic stem cell types into gastrulating embryo-like structures

(2018) Berna Sozen et al.   NATURE CELL BIOLOGY

Should the 14‐day rule for embryo research become the 28‐day rule?

(2018) John B Appleby et al.   EMBO Molecular Medicine

Self-organization of a human organizer by combined Wnt and Nodal signalling

(2018) I. Martyn et al.   NATURE

Opening the black box: Stem cell–based modeling of human post-implantation development

(2018) Kenichiro Taniguchi et al.   JOURNAL OF CELL BIOLOGY

Multi-axial self-organization properties of mouse embryonic stem cells into gastruloids

(2018) Leonardo Beccari et al.   NATURE

WNT signaling memory is required for ACTIVIN to function as a morphogen in human gastruloids

(2018) Anna Yoney et al.   eLife

Principles of early human development and germ cell program from conserved model systems

(2017) Toshihiro Kobayashi et al.   NATURE

Assembly of embryonic and extraembryonic stem cells to mimic embryogenesis in vitro

(2017) Sarah Ellys Harrison et al.   SCIENCE

A pluripotent stem cell-based model for post-implantation human amniotic sac development

(2017) Yue Shao et al.   Nature Communications

Addressing the ethical issues raised by synthetic human entities with embryo-like features

(2017) John Aach et al.   eLife

Single-Cell RNA-Seq Reveals Lineage and X Chromosome Dynamics in Human Preimplantation Embryos

(2016) Sophie Petropoulos et al.   CELL

A Balance between Secreted Inhibitors and Edge Sensing Controls Gastruloid Self-Organization

(2016) Fred Etoc et al.   DEVELOPMENTAL CELL

The Germ Cell Fate of Cynomolgus Monkeys Is Specified in the Nascent Amnion

(2016) Kotaro Sasaki et al.   DEVELOPMENTAL CELL

Implantation barrier overcome

(2016) Janet Rossant   NATURE

Embryology policy: Revisit the 14-day rule

(2016) Insoo Hyun et al.   NATURE

Self-organization of the in vitro attached human embryo

(2016) Alessia Deglincerti et al.   NATURE

Self-organization of the human embryo in the absence of maternal tissues

(2016) Marta N. Shahbazi et al.   NATURE CELL BIOLOGY

Self-organization of human embryonic stem cells on micropatterns

(2016) Alessia Deglincerti et al.   Nature Protocols

Specification and epigenetic programming of the human germ line

(2016) Walfred W. C. Tang et al.   NATURE REVIEWS GENETICS

The Microenvironment of Human Implantation: Determinant of Reproductive Success

(2015) Lois A. Salamonsen et al.   AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY

Defining the three cell lineages of the human blastocyst by single-cell RNA-seq

(2015) P. Blakeley et al.   DEVELOPMENT

Establishment of a novel human embryonic stem cell-derived trophoblastic spheroid implantation model

(2015) Yin-Lau Lee et al.   HUMAN REPRODUCTION

What if stem cells turn into embryos in a dish?

(2015) Martin F Pera et al.   NATURE METHODS

What is a human embryo? A new piece in the bioethics puzzle

(2015) Ińigo de Miguel Beriain   CROATIAN MEDICAL JOURNAL

Symmetry breaking, germ layer specification and axial organisation in aggregates of mouse embryonic stem cells

(2014) S. C. van den Brink et al.   DEVELOPMENT

Cyclic Decidualization of the Human Endometrium in Reproductive Health and Failure

(2014) Birgit Gellersen et al.   ENDOCRINE REVIEWS

A method to recapitulate early embryonic spatial patterning in human embryonic stem cells

(2014) Aryeh Warmflash et al.   NATURE METHODS

In vitro culture of mouse blastocysts beyond the implantation stages

(2014) Ivan Bedzhov et al.   Nature Protocols

Skepticism About the “Convertibility” of Induced Pluripotent Stem Cells

(2013) Thomas V. Cunningham   AMERICAN JOURNAL OF BIOETHICS

The Embryo Potentiality Argument Revisited: “Once More Unto the Breach, Dear Friends”

(2013) Insoo Hyun   AMERICAN JOURNAL OF BIOETHICS

The Argument from Potentiality in the Embryo Protection Debate: Finally “Depotentialized”?

(2013) Marco Stier et al.   AMERICAN JOURNAL OF BIOETHICS

Human Embryonic Stem Cells Derived by Somatic Cell Nuclear Transfer

(2013) Masahito Tachibana et al.   CELL

Derivation of human embryonic stem cells using a post–inner cell mass intermediate

(2013) Thomas O'Leary et al.   Nature Protocols

Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells

(2013) Liying Yan et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Defective Soil for a Fertile Seed? Altered Endometrial Development Is Detrimental to Pregnancy Success

(2013) Jemma Evans et al.   PLoS One

In-vitro model systems for the study of human embryo–endometrium interactions

(2013) Charlotte H.E. Weimar et al.   REPRODUCTIVE BIOMEDICINE ONLINE

Molecular aspects of implantation failure

(2012) Y.E.M. Koot et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

The roles of FGF and MAP kinase signaling in the segregation of the epiblast and hypoblast cell lineages in bovine and human embryos

(2012) E. W. Kuijk et al.   DEVELOPMENT

Analysis of human embryos from zygote to blastocyst reveals distinct gene expression patterns relative to the mouse

(2012) Kathy K. Niakan et al.   DEVELOPMENTAL BIOLOGY

Embryonic stem cell potency fluctuates with endogenous retrovirus activity

(2012) Todd S. Macfarlan et al.   NATURE

Tracking the progression of the human inner cell mass during embryonic stem cell derivation

(2012) Thomas O'Leary et al.   NATURE BIOTECHNOLOGY

Mechanisms of implantation: strategies for successful pregnancy

(2012) Jeeyeon Cha et al.   NATURE MEDICINE

Self-Organization Phenomena in Embryonic Stem Cell-Derived Embryoid Bodies: Axis Formation and Breaking of Symmetry during Cardiomyogenesis

(2011) Christiane Fuchs et al.   CELLS TISSUES ORGANS

Waves of early transcriptional activation and pluripotency program initiation during human preimplantation development

(2011) R. Vassena et al.   DEVELOPMENT

Human hypoblast formation is not dependent on FGF signalling

(2011) Mila Roode et al.   DEVELOPMENTAL BIOLOGY

Origin and role of distal visceral endoderm, a group of cells that determines anterior–posterior polarity of the mouse embryo

(2011) Katsuyoshi Takaoka et al.   NATURE CELL BIOLOGY

Molecular and Functional Characterizations of Gastrula Organizer Cells Derived from Human Embryonic Stem Cells

(2011) Nadav Sharon et al.   STEM CELLS

Transcriptomic landscape of the primitive streak

(2010) C. Alev et al.   DEVELOPMENT

On the origin of amniotic stem cells: of mice and men

(2010) Mariya P. Dobreva et al.   INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY

Analysis of co-expression of OCT4, NANOG and SOX2 in pluripotent cells of the porcine embryo, in vivo and in vitro

(2010) Leonie du Puy et al.   THERIOGENOLOGY

Aggregated P19 mouse embryonal carcinoma cells as a simple in vitro model to study the molecular regulations of mesoderm formation and axial elongation morphogenesis

(2009) Yusuke Marikawa et al.   GENESIS

Wnt Signaling Mediates Self-Organization and Axis Formation in Embryoid Bodies

(2008) Derk ten Berge et al.   Cell Stem Cell

Micropatterning of human embryonic stem cells dissects the mesoderm and endoderm lineages

(2008) Lawrence Haoran Lee et al.   Stem Cell Research