Preservation of protein expression systems at elevated temperatures for portable therapeutic production

Portable, On-Demand Biomolecular Manufacturing

(2016) Keith Pardee et al.   CELL

Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components

(2016) Keith Pardee et al.   CELL

Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes inEscherichia coli

(2016) Brady F. Cress et al.   NUCLEIC ACIDS RESEARCH

A bacterium that degrades and assimilates poly(ethylene terephthalate)

(2016) Shosuke Yoshida et al.   SCIENCE

The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology

(2016) Jonathan Garamella et al.   ACS Synthetic Biology

LyophilizedEscherichia coli-based cell-free systems for robust, high-density, long-term storage

(2016) Mark Thomas Smith et al.   BIOTECHNIQUES

Cell-free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just-add-water cell-free system

(2015) Amin S. M. Salehi et al.   Biotechnology Journal

A cell-free expression and purification process for rapid production of protein biologics

(2015) Challise J. Sullivan et al.   Biotechnology Journal

Creating a completely “cell-free” system for protein synthesis

(2015) Mark Thomas Smith et al.   BIOTECHNOLOGY PROGRESS

Modular microfluidics for point-of-care protein purifications

(2015) L. J. Millet et al.   LAB ON A CHIP

Cell-free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just-add-water cell-free system

(2015) Amin S. M. Salehi et al.   Biotechnology Journal

A cell-free expression and purification process for rapid production of protein biologics

(2015) Challise J. Sullivan et al.   Biotechnology Journal

Biocatalytic Production of Trehalose from Maltose by Using Whole Cells of Permeabilized Recombinant Escherichia coli

(2015) Zhaojuan Zheng et al.   PLoS One

Paper-Based Synthetic Gene Networks

(2014) Keith Pardee et al.   CELL

Anhydrobiosis: Drying Out with Sugar

(2014) Kevin A. Morano   CURRENT BIOLOGY

Gene Circuit Performance Characterization and Resource Usage in a Cell-Free “Breadboard”

(2014) Dan Siegal-Gaskins et al.   ACS Synthetic Biology

Stochasticity in Gene Expression in a Cell-Sized Compartment

(2014) Kazuya Nishimura et al.   ACS Synthetic Biology

Pore-forming pyocin S5 utilizes the FptA ferripyochelin receptor to kill Pseudomonas aeruginosa

(2013) A. Elfarash et al.   MICROBIOLOGY-SGM

Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology

(2013) James Chappell et al.   NUCLEIC ACIDS RESEARCH

Multi-Input Regulation and Logic with T7 Promoters in Cells and Cell-Free Systems

(2013) Sukanya Iyer et al.   PLoS One

Implementation of cell-free biological networks at steady state

(2013) H. Niederholtmeyer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants

(2013) D. L. Shis et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Thrombin-Mediated Transcriptional Regulation Using DNA Aptamers in DNA-Based Cell-Free Protein Synthesis

(2013) Sukanya Iyer et al.   ACS Synthetic Biology

Probing Cell-Free Gene Expression Noise in Femtoliter Volumes

(2013) David K. Karig et al.   ACS Synthetic Biology

Strain-dependent diversity in the Pseudomonas aeruginosa quorum-sensing regulon

(2012) S. Chugani et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genome Replication, Synthesis, and Assembly of the Bacteriophage T7 in a Single Cell-Free Reaction

(2012) Jonghyeon Shin et al.   ACS Synthetic Biology

Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines

(2011) James F. Zawada et al.   BIOTECHNOLOGY AND BIOENGINEERING

Trehalose: Current Use and Future Applications

(2011) Satoshi Ohtake et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Expression optimization and synthetic gene networks in cell-free systems

(2011) David K. Karig et al.   NUCLEIC ACIDS RESEARCH

An E. coli Cell-Free Expression Toolbox: Application to Synthetic Gene Circuits and Artificial Cells

(2011) Jonghyeon Shin et al.   ACS Synthetic Biology

A predicted S-type pyocin shows a bactericidal activity against clinicalPseudomonas aeruginosaisolates through membrane damage

(2010) Hua Ling et al.   FEBS LETTERS

Ribosomal synthesis and in situ isolation of peptide molecules in a cell-free translation system

(2010) Kyung-Ho Lee et al.   PROTEIN EXPRESSION AND PURIFICATION

Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances

(2008) Irith Wiegand et al.   Nature Protocols

Cohesin subunit SMC1 associates with mitotic microtubules at the spindle pole

(2008) R. W. Wong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Efficient cell-free production of olfactory receptors: Detergent optimization, structure, and ligand binding analyses

(2008) L. Kaiser et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Escherichia coli-based cell-free synthesis of virus-like particles

(2007) Bradley C. Bundy et al.   BIOTECHNOLOGY AND BIOENGINEERING