Current advances in the algae-made biopharmaceuticals field

N-glycans of the microalga Chlorella vulgaris are of the oligomannosidic type but highly methylated

(2019) Réka Mócsai et al.   Scientific Reports

An evaluation of microalgae as a recombinant protein oral delivery platform for fish using green fluorescent protein (GFP)

(2019) Kwang-Chul Kwon et al.   FISH & SHELLFISH IMMUNOLOGY

Immunogenicity and safety of a quadrivalent plant-derived virus like particle influenza vaccine candidate—Two randomized Phase II clinical trials in 18 to 49 and ≥50 years old adults

(2019) Stéphane Pillet et al.   PLoS One

Identification and characterisation of the novel endogenous promoter HASP1 and its signal peptide from Phaeodactylum tricornutum

(2019) Erdenedolgor Erdene-Ochir et al.   Scientific Reports

Separation Options for Phosphorylated Osteopontin from Transgenic Microalgae Chlamydomonas reinhardtii

(2018) Ayswarya Ravi et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Development of an alcohol-inducible gene expression system for recombinant protein expression in Chlamydomonas reinhardtii

(2018) Sujin Lee et al.   JOURNAL OF APPLIED PHYCOLOGY

Multimer of the antimicrobial peptide Mytichitin-A expressed in Chlamydomonas reinhardtii exerts a broader antibacterial spectrum and increased potency

(2018) Bin Dong et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Engineering the chloroplast of Chlamydomonas reinhardtii to express the recombinant PfCelTOS-Il2 antigen-adjuvant fusion protein

(2018) Shabnam Shamriz et al.   JOURNAL OF BIOTECHNOLOGY

Expression of a Zika virus antigen in microalgae: towards mucosal vaccine development

(2018) Verónica Araceli Márquez-Escobar et al.   JOURNAL OF BIOTECHNOLOGY

The algal chloroplast as a synthetic biology platform for production of therapeutic proteins

(2018) Yuliya M. Dyo et al.   MICROBIOLOGY-SGM

Efficient Expression of an Alzheimer’s Disease Vaccine Candidate in the Microalga Schizochytrium sp. Using the Algevir System

(2018) Benita Ortega-Berlanga et al.   MOLECULAR BIOTECHNOLOGY

Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii

(2018) Thomas Baier et al.   NUCLEIC ACIDS RESEARCH

Optimising light conditions increases recombinant protein production in Chlamydomonas reinhardtii chloroplasts

(2018) Saskya E. Carrera Pacheco et al.   Algal Research-Biomass Biofuels and Bioproducts

Chloroplast engineering of Chlamydomonas reinhardtii to use phosphite as phosphorus source

(2018) José M. Sandoval-Vargas et al.   Algal Research-Biomass Biofuels and Bioproducts

Humoral and cell-mediated immune responses to H5N1 plant-made virus-like particle vaccine are differentially impacted by alum and GLA-SE adjuvants in a Phase 2 clinical trial

(2018) Stéphane Pillet et al.   npj Vaccines

Safety and immunogenicity of a plant-produced Pfs25 virus-like particle as a transmission blocking vaccine against malaria: A Phase 1 dose-escalation study in healthy adults

(2018) Jessica A. Chichester et al.   VACCINE

Recombinant Antibody Production in CHO and NS0 Cells: Differences and Similarities

(2018) Venkata Gayatri Dhara et al.   BIODRUGS

Cell culture media for recombinant protein expression in Chinese hamster ovary (CHO) cells: History, key components, and optimization strategies

(2018) Frank V. Ritacco et al.   BIOTECHNOLOGY PROGRESS

Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms

(2018) Ryuta Wada et al.   mAbs

Engineered Fusion Proteins for Efficient Protein Secretion and Purification of a Human Growth Factor from the Green Microalga Chlamydomonas reinhardtii

(2018) Thomas Baier et al.   ACS Synthetic Biology

Prospects on the Use of Schizochytrium sp. to Develop Oral Vaccines

(2018) Abel Ramos-Vega et al.   Frontiers in Microbiology

Expression and Characterization of Functional Recombinant Bet v 1.0101 in the Chloroplast of Chlamydomonas reinhardtii

(2017) Sonja Hirschl et al.   INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY

Synthesis of bacteriophage lytic proteins against Streptococcus pneumoniae in the chloroplast of Chlamydomonas reinhardtii

(2017) Laura Stoffels et al.   PLANT BIOTECHNOLOGY JOURNAL

High-yield secretion of recombinant proteins from the microalga Chlamydomonas reinhardtii

(2017) Erick Miguel Ramos-Martinez et al.   PLANT BIOTECHNOLOGY JOURNAL

Alga-Made Anti-Hepatitis B Antibody Binds to Human Fcγ Receptors

(2017) Gaëtan Vanier et al.   Biotechnology Journal

New tools for chloroplast genetic engineering allow the synthesis of human growth hormone in the green alga Chlamydomonas reinhardtii

(2016) Thanyanan Wannathong et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Towards autotrophic tissue engineering: Photosynthetic gene therapy for regeneration

(2016) Myra Noemi Chávez et al.   BIOMATERIALS

Recombinant pharmaceuticals from microbial cells: a 2015 update

(2016) Laura Sanchez-Garcia et al.   Microbial Cell Factories

Immunotherapy using algal-produced Ara h 1 core domain suppresses peanut allergy in mice

(2016) James A. Gregory et al.   PLANT BIOTECHNOLOGY JOURNAL

A novel genetic engineering platform for the effective management of biological contaminants for the production of microalgae

(2016) Maribel M. Loera-Quezada et al.   PLANT BIOTECHNOLOGY JOURNAL

Chlamydomonas reinhardtii chloroplasts express an orally immunogenic protein targeting the p210 epitope implicated in atherosclerosis immunotherapies

(2016) Josué I. Beltrán-López et al.   PLANT CELL REPORTS

Efficient expression of nuclear transgenes in the green alga Chlamydomonas: synthesis of an HIV antigen and development of a new selectable marker

(2016) Rouhollah Barahimipour et al.   PLANT MOLECULAR BIOLOGY

UV-mediated Chlamydomonas mutants with enhanced nuclear transgene expression by disruption of DNA methylation-dependent and independent silencing systems

(2016) Sari Dewi Kurniasih et al.   PLANT MOLECULAR BIOLOGY

Biosimilars: A consideration of the regulations in the United States and European union

(2016) Justin Daller   REGULATORY TOXICOLOGY AND PHARMACOLOGY

Plant-produced biopharmaceuticals: A case of technical developments driving clinical deployment

(2016) G. P. Lomonossoff et al.   SCIENCE

Transgene Expression in Microalgae—From Tools to Applications

(2016) Lior Doron et al.   Frontiers in Plant Science

Chemical synthesis of erythropoietin glycoforms for insights into the relationship between glycosylation pattern and bioactivity

(2016) M. Murakami et al.   Science Advances

Low cost industrial production of coagulation factor IX bioencapsulated in lettuce cells for oral tolerance induction in hemophilia B

(2015) Jin Su et al.   BIOMATERIALS

Molecular pharming’s foot in the FDA’s door: Protalix’s trailblazing story

(2015) Tsafrir S. Mor   BIOTECHNOLOGY LETTERS

Manipulating the glycosylation pathway in bacterial and lower eukaryotes for production of therapeutic proteins

(2015) Diana Chinyere Anyaogu et al.   CURRENT OPINION IN BIOTECHNOLOGY

The importance of patents to innovation: updated cross-industry comparisons with biopharmaceuticals

(2015) Iain Cockburn et al.   EXPERT OPINION ON THERAPEUTIC PATENTS

Investigating the dynamics of recombinant protein secretion from a microalgal host

(2015) Kyle J. Lauersen et al.   JOURNAL OF BIOTECHNOLOGY

In Metabolic Engineering of Eukaryotic Microalgae: Potential and Challenges Come with Great Diversity

(2015) Javier A. Gimpel et al.   Frontiers in Microbiology

Cyanobacteria and microalgae: a renewable source of bioactive compounds and other chemicals

(2015) Telma Encarnação et al.   SCIENCE PROGRESS

Production of recombinant proteins in microalgae at pilot greenhouse scale

(2014) Javier A. Gimpel et al.   BIOTECHNOLOGY AND BIOENGINEERING

Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses

(2014) Beth A. Rasala et al.   PHOTOSYNTHESIS RESEARCH

Algal chloroplast produced camelid VH H antitoxins are capable of neutralizing botulinum neurotoxin

(2014) Daniel J. Barrera et al.   PLANT BIOTECHNOLOGY JOURNAL

Enhanced Genetic Tools for Engineering Multigene Traits into Green Algae

(2014) Beth A. Rasala et al.   PLoS One

Alga-Produced Cholera Toxin-Pfs25 Fusion Proteins as Oral Vaccines

(2013) James A. Gregory et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Ice recrystallization inhibition mediated by a nuclear-expressed and -secreted recombinant ice-binding protein in the microalga Chlamydomonas reinhardtii

(2013) Kyle J. Lauersen et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Preparation of transgenic Dunaliella salina for immunization against white spot syndrome virus in crayfish

(2013) Shuying Feng et al.   ARCHIVES OF VIROLOGY

Production of anti-cancer immunotoxins in algae: Ribosome inactivating proteins as fusion partners

(2013) Miller Tran et al.   BIOTECHNOLOGY AND BIOENGINEERING

Life based on phosphite: a genome-guided analysis of Desulfotignum phosphitoxidans

(2013) Anja Poehlein et al.   BMC GENOMICS

Future directions for the development ofChlamydomonas-based vaccines

(2013) Sergio Rosales-Mendoza   Expert Review of Vaccines

Expression of an HBcAg-based antigen carrying angiotensin II in Chlamydomonas reinhardtii as a candidate hypertension vaccine

(2013) Ruth E. Soria-Guerra et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Vaccination against Influenza with Recombinant Hemagglutinin Expressed by Schizochytrium sp. Confers Protective Immunity

(2013) Anne-Cécile V. Bayne et al.   PLoS One

A Chlamydomonas-Derived Human Papillomavirus 16 E7 Vaccine Induces Specific Tumor Protection

(2013) Olivia C. Demurtas et al.   PLoS One

Evaluating nuclear transgene expression systems in Chlamydomonas reinhardtii

(2013) Anil Kumar et al.   Algal Research-Biomass Biofuels and Bioproducts

Food commodities from microalgae

(2012) René B Draaisma et al.   CURRENT OPINION IN BIOTECHNOLOGY

Expression of soybean Kunitz trypsin inhibitor gene SKTI in Dunaliella salina

(2012) Xiao-Jie Chai et al.   JOURNAL OF APPLIED PHYCOLOGY

An engineered diatom acting like a plasma cell secreting human IgG antibodies with high efficiency

(2012) Franziska Hempel et al.   Microbial Cell Factories

Algae-Produced Pfs25 Elicits Antibodies That Inhibit Malaria Transmission

(2012) James A. Gregory et al.   PLoS One

Robust Expression and Secretion of Xylanase1 in Chlamydomonas reinhardtii by Fusion to a Selection Gene and Processing with the FMDV 2A Peptide

(2012) Beth A. Rasala et al.   PLoS One

Production of unique immunotoxin cancer therapeutics in algal chloroplasts

(2012) M. Tran et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Posterior decompression and short segmental pedicle screw fixation combined with vertebroplasty for Kümmell’s disease with neurological deficits

(2012) GUANG-QUAN ZHANG et al.   Experimental and Therapeutic Medicine

A new inducible expression system in a transformed green alga, Chlorella vulgaris

(2011) Y.F. Niu et al.   GENETICS AND MOLECULAR RESEARCH

Protein N-glycosylation, protein folding, and protein quality control

(2011) Jürgen Roth et al.   MOLECULES AND CELLS

Algae as Protein Factories: Expression of a Human Antibody and the Respective Antigen in the Diatom Phaeodactylum tricornutum

(2011) Franziska Hempel et al.   PLoS One

High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp.

(2011) O. Kilian et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Plant glycans: friend or foe in vaccine development?

(2010) Dirk Bosch et al.   Expert Review of Vaccines

Enhanced chloroplast transgene expression in a nuclear mutant of Chlamydomonas

(2010) Laure Michelet et al.   PLANT BIOTECHNOLOGY JOURNAL

Plant-specific glycosylation patterns in the context of therapeutic protein production

(2010) Véronique Gomord et al.   PLANT BIOTECHNOLOGY JOURNAL

Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii

(2010) Beth A. Rasala et al.   PLANT BIOTECHNOLOGY JOURNAL

Engineering the Chloroplast Targeted Malarial Vaccine Antigens in Chlamydomonas Starch Granules

(2010) David Dauvillée et al.   PLoS One

Solar-powered factories for new vaccines and antibiotics

(2010) Ralph Bock et al.   TRENDS IN BIOTECHNOLOGY

Heat-stable oral alga-based vaccine protects mice from Staphylococcus aureus infection

(2009) Imke A.J. Dreesen et al.   JOURNAL OF BIOTECHNOLOGY

Strategies to facilitate transgene expression in Chlamydomonas reinhardtii

(2009) Alke Eichler-Stahlberg et al.   PLANTA

A novel expression platform for the production of diabetes-associated autoantigen human glutamic acid decarboxylase (hGAD65)

(2008) Xiaofeng Wang et al.   BMC BIOTECHNOLOGY

A codon-optimized luciferase from Gaussia princeps facilitates the in vivo monitoring of gene expression in the model alga Chlamydomonas reinhardtii

(2008) Ning Shao et al.   CURRENT GENETICS

Transgenic microalgae as a non-antibiotic bactericide producer to defend against bacterial pathogen infection in the fish digestive tract

(2008) Si-Shen Li et al.   FISH & SHELLFISH IMMUNOLOGY

(Re)defining biopharmaceutical

(2008) Ronald A Rader   NATURE BIOTECHNOLOGY

Generation ofChlamydomonasstrains that efficiently express nuclear transgenes

(2008) Juliane Neupert et al.   PLANT JOURNAL