High-yield secretion of recombinant proteins from the microalga Chlamydomonas reinhardtii

Pilot-scale cultivation of wall-deficient transgenic Chlamydomonas reinhardtii strains expressing recombinant proteins in the chloroplast

(2016) Julie A. Z. Zedler et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Towards autotrophic tissue engineering: Photosynthetic gene therapy for regeneration

(2016) Myra Noemi Chávez et al.   BIOMATERIALS

UV-mediated Chlamydomonas mutants with enhanced nuclear transgene expression by disruption of DNA methylation-dependent and independent silencing systems

(2016) Sari Dewi Kurniasih et al.   PLANT MOLECULAR BIOLOGY

Synthetic promoters capable of driving robust nuclear gene expression in the green alga Chlamydomonas reinhardtii

(2016) Melissa A. Scranton et al.   Algal Research-Biomass Biofuels and Bioproducts

Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit

(2015) Kyle J. Lauersen et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Genetic tools and techniques for Chlamydomonas reinhardtii

(2015) Jan H. Mussgnug   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

High-yield secretion of recombinant proteins expressed in tobacco cell culture with a designer glycopeptide tag: Process development

(2015) Ningning Zhang et al.   Biotechnology Journal

A new approach to express transgenes in microalgae and its use to increase the flocculation ability of Chlamydomonas reinhardtii

(2015) Encarnación Díaz-Santos et al.   JOURNAL OF APPLIED PHYCOLOGY

Robust expression of heterologous genes by selection marker fusion system in improved Chlamydomonas strains

(2015) Fantao Kong et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Investigating the dynamics of recombinant protein secretion from a microalgal host

(2015) Kyle J. Lauersen et al.   JOURNAL OF BIOTECHNOLOGY

Biotechnological exploitation of microalgae

(2015) Doris Gangl et al.   JOURNAL OF EXPERIMENTAL BOTANY

Molecular techniques to interrogate and edit theChlamydomonasnuclear genome

(2015) Robert E. Jinkerson et al.   PLANT JOURNAL

Dissecting the contributions of GC content and codon usage to gene expression in the model algaChlamydomonas reinhardtii

(2015) Rouhollah Barahimipour et al.   PLANT JOURNAL

Improved and versatile viral 2A platforms for dependable and inducible high-level expression of dicistronic nuclear genes in Chlamydomonas reinhardtii

(2015) Thomas M. Plucinak et al.   PLANT JOURNAL

Designer diatom episomes delivered by bacterial conjugation

(2015) Bogumil J. Karas et al.   Nature Communications

High-yield secretion of recombinant proteins expressed in tobacco cell culture with a designer glycopeptide tag: Process development

(2015) Ningning Zhang et al.   Biotechnology Journal

A secretory kinase complex regulates extracellular protein phosphorylation

(2015) Jixin Cui et al.   eLife

Production of recombinant proteins in microalgae at pilot greenhouse scale

(2014) Javier A. Gimpel et al.   BIOTECHNOLOGY AND BIOENGINEERING

Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses

(2014) Beth A. Rasala et al.   PHOTOSYNTHESIS RESEARCH

Protein N-glycosylation in eukaryotic microalgae and its impact on the production of nuclear expressed biopharmaceuticals

(2014) Elodie Mathieu-Rivet et al.   Frontiers in Plant Science

Production of therapeutic proteins in the chloroplast of Chlamydomonas reinhardtii

(2014) Alma Lorena Almaraz-Delgado et al.   AMB Express

Ice recrystallization inhibition mediated by a nuclear-expressed and -secreted recombinant ice-binding protein in the microalga Chlamydomonas reinhardtii

(2013) Kyle J. Lauersen et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Functional Identification of a Hydroxyproline-O-galactosyltransferase Specific for Arabinogalactan Protein Biosynthesis inArabidopsis

(2013) Debarati Basu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Exploring theN-glycosylation Pathway inChlamydomonas reinhardtiiUnravels Novel Complex Structures

(2013) Elodie Mathieu-Rivet et al.   MOLECULAR & CELLULAR PROTEOMICS

Identification of three hydroxyproline O-arabinosyltransferases in Arabidopsis thaliana

(2013) Mari Ogawa-Ohnishi et al.   Nature Chemical Biology

The cell-wall glycoproteins of the green alga Scenedesmus obliquus. The predominant cell-wall polypeptide of Scenedesmus obliquus is related to the cell-wall glycoprotein gp3 of Chlamydomonas reinhardtii

(2013) Jürgen Voigt et al.   PLANT SCIENCE

Genetic engineering of algal chloroplasts: Progress and prospects

(2013) S. Purton et al.   RUSSIAN JOURNAL OF PLANT PHYSIOLOGY

The sweet tooth of biopharmaceuticals: Importance of recombinant protein glycosylation analysis

(2012) Nico Lingg et al.   Biotechnology Journal

Efficient recombinant protein production and secretion from nuclear transgenes in Chlamydomonas reinhardtii

(2012) Kyle J. Lauersen et al.   JOURNAL OF BIOTECHNOLOGY

An engineered diatom acting like a plasma cell secreting human IgG antibodies with high efficiency

(2012) Franziska Hempel et al.   Microbial Cell Factories

Robust Expression and Secretion of Xylanase1 in Chlamydomonas reinhardtii by Fusion to a Selection Gene and Processing with the FMDV 2A Peptide

(2012) Beth A. Rasala et al.   PLoS One

SignalP 4.0: discriminating signal peptides from transmembrane regions

(2011) Thomas Nordahl Petersen et al.   NATURE METHODS

Micro-algae come of age as a platform for recombinant protein production

(2010) Elizabeth Specht et al.   BIOTECHNOLOGY LETTERS

Plant-specific glycosylation patterns in the context of therapeutic protein production

(2010) Véronique Gomord et al.   PLANT BIOTECHNOLOGY JOURNAL

Human growth hormone expressed in tobacco cells as an arabinogalactan-protein fusion glycoprotein has a prolonged serum life

(2010) Jianfeng Xu et al.   TRANSGENIC RESEARCH

Production of recombinant proteins by microbes and higher organisms

(2009) Arnold L. Demain et al.   BIOTECHNOLOGY ADVANCES

Carbohydrate analysis throughout the development of a protein therapeutic

(2009) Elizabeth Higgins   GLYCOCONJUGATE JOURNAL

Strategies to facilitate transgene expression in Chlamydomonas reinhardtii

(2009) Alke Eichler-Stahlberg et al.   PLANTA

Chlamydomonas (Chlorophyceae) colony PCR

(2009) Muqing Cao et al.   PROTOPLASMA

A codon-optimized luciferase from Gaussia princeps facilitates the in vivo monitoring of gene expression in the model alga Chlamydomonas reinhardtii

(2008) Ning Shao et al.   CURRENT GENETICS

Effects of glycosylation on the stability of protein pharmaceuticals

(2008) Ricardo J. Solá et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Gaussia-luciferase as a sensitive reporter gene for monitoring promoter activity in the nucleus of the green alga Chlamydomonas reinhardtii

(2008) Ovidiu Ruecker et al.   MOLECULAR GENETICS AND GENOMICS

The O-Hyp glycosylation code in tobacco and Arabidopsis and a proposed role of Hyp-glycans in secretion

(2008) Jianfeng Xu et al.   PHYTOCHEMISTRY

Generation ofChlamydomonasstrains that efficiently express nuclear transgenes

(2008) Juliane Neupert et al.   PLANT JOURNAL