Article
Chemistry, Multidisciplinary
Momoko Akiyama, Ryosuke Ueki, Masataka Yanagawa, Mitsuhiro Abe, Michio Hiroshima, Yasushi Sako, Shinsuke Sando
Summary: In this study, a synthetic receptor agonist was designed using a DNA aptamer as a building block to fine-tune agonism. The developed synthetic partial agonist can regulate therapeutically relevant cellular activities by eliciting finely-tuned receptor signaling.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Engineering, Biomedical
Rocco Latorre, Paulina D. Ramirez-Garcia, Alan Hegron, James L. Grace, Jeffri S. Retamal, Priyank Shenoy, Mai Tran, Luigi Aurelio, Bernard Flynn, Daniel P. Poole, Rafael Klein-Cloud, Dane D. Jensen, Thomas P. Davis, Brian L. Schmidt, John F. Quinn, Michael R. Whittaker, Nicholas A. Veldhuis, Nigel W. Bunnett
Summary: Stable star polymer nanostars that release antagonists continuously for 24 hours provide effective and long-lasting relief from chronic pain by disrupting endosomal signaling.
Article
Chemistry, Multidisciplinary
Hui Chen, Wanyu Xu, Hui Shi, Yan Qiao, Xiaoxiao He, Jing Zheng, Shaohong Zhou, Xiaohai Yang, Kemin Wang, Jianbo Liu
Summary: In this study, an artificial transmembrane signal transduction system using DNA-based artificial membrane receptors is described. Through low pH-mediated formation of the i-motif and dimerization of the receptors, fluorescence resonance energy transfer and G-quadruplex/hemin-mediated fluorescence amplification are activated within giant unilamellar vesicles. The study also establishes an intercellular signal communication model using coacervate microdroplets, which activate the dimerization of artificial receptors and subsequent fluorescence production or polymerization in giant unilamellar vesicles.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Naoya Kawakami, Hiroki Sato, Naohiro Terasaka, Kunio Matsumoto, Hiroaki Suga
Summary: The researchers have developed a ubiquitin-based protein ligand multimer, called U-bodies, as a surrogate agonist for the MET receptor. These U-body constructs have high binding affinity, thermal stability, and proteolytic stability. By assembling them into dimeric, tetrameric, and octameric structures, potent MET activation and cell proliferation-promoting activities are achieved.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Biochemistry & Molecular Biology
Xin Zhuo, Yue Wu, Xiujuan Fu, Xiaoyu Liang, Yuxin Xiang, Jianbin Li, Canquan Mao, Yuhong Jiang
Summary: Inflammatory diseases are increasing globally, and COVID-19 also exhibits hyperinflammatory responses. Protease-activated receptors (PARs) are popular drug targets involved in regulating inflammatory responses in multiple systems. PARs activation has both anti-inflammatory and pro-inflammatory effects depending on the context of inflammation. Recent findings suggest that PARs also participate in COVID-19-related inflammatory cytokine release.
Review
Biochemistry & Molecular Biology
Elena Cojocaru, Cristian Cojocaru, Cristiana-Elena Vlad, Lucian Eva
Summary: The renin-angiotensin system (RAS) presents a major challenge in cardiovascular medicine, with discussions focusing on its role in the chronic progression of SARS-CoV-2 infection. SARS-CoV-2 disrupts the balance of RAS pathways, leading to myocardial fibrous remodeling, thromboembolic processes, and reduced organ perfusion. Studies have examined the pathogenic role of ACE2 and the mechanisms of RAS involvement in COVID cardiovascular injuries, as well as potential therapeutic directions for long COVID-related cardiovascular damage.
Article
Biochemistry & Molecular Biology
Elizabet Monteagudo-Cascales, Alvaro Ortega, Felix Velando, Bertrand Morel, Miguel A. Matilla, Tino Krell
Summary: Bacterial signal transduction systems are typically activated by the binding of signal molecules to receptor ligand binding domains (LBDs), such as the NIT LBD. The NIT domain is found in over 15,000 receptors from 30 bacterial phyla and 19 eukaryotic phyla, and it is a part of seven receptor families involved in transcription control, chemotaxis, or second messenger regulation.
MOLECULAR MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Mariam Ahmed Galal, Samhar Samer Alouch, Buthainah Saad Alsultan, Huda Dahman, Nouf Abdullah Alyabis, Sarah Ammar Alammar, Ahmad Aljada
Summary: This comprehensive review thoroughly explores the intricate involvement of insulin receptor isoforms and insulin-like growth factor receptors in the insulin and insulin-like growth factor signaling pathway, emphasizing their crucial roles in cancer development and resistance to anti-cancer drugs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Pere Monge, Kaja Borup Lovschall, Ane Bretschneider Sogaard, Raoul Walther, Thaddeus W. Golbek, Lars Schmuser, Tobias Weidner, Alexander N. Zelikin
Summary: A fully synthetic, chemical apoptosis-inducing receptor (AIR) molecule has been designed to be anchored into the lipid bilayer of cells and release a highly potent toxin in response to incoming biological input. The synthetic receptor has four elements and can be installed into cells with minimal handling, remaining dormant until external activation triggers the release of the toxin. Activation of the receptor in 3D cell culture remains accessible for at least 5 days, outperforming other state of the art receptor designs.
Review
Allergy
Francesca Levi-Schaffer, Bernhard F. Gibbs, Jenny Hallgren, Carlo Pucillo, Frank Redegeld, Frank Siebenhaar, Joana Vitte, Soraya Mezouar, Moiese Michel, Pier Giorgio Puzzovio, Marcus Maurer
Summary: With the advancement of research technologies and methodologies, there has been an increased understanding of the origin, development, function, and interactions of human mast cells. The potential contribution of mast cells to disease has led to the development of novel therapeutic approaches.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Patrick Haider, Julia B. Kral-Pointner, Manuel Salzmann, Florian Moik, Sonja Bleichert, Waltraud C. Schrottmaier, Christoph Kaun, Mira Brekalo, Michael B. Fischer, Walter S. Speidl, Christian Hengstenberg, Bruno K. Podesser, Kurt Huber, Ingrid Pabinger, Sylvia Knapp, Frank Brombacher, Christine Brostjan, Cihan Ay, Johann Wojta, Philipp J. Hohensinner
Summary: IL-4R alpha plays a homeostatic role in regulating the lifespan of monocytes, and its absence leads to a reduction in monocyte numbers and affects the occurrence of inflammatory responses.
Review
Biochemistry & Molecular Biology
Shanshan Song, Zhangping Xiao, Frank J. Dekker, Gerrit J. Poelarends, Barbro N. Melgert
Summary: The family of macrophage migration inhibitory factor (MIF) proteins in humans consists of MIF, D-dopachrome tautomerase (D-DT), and DDT-like (DDTL). MIF has various effects on cellular processes, while the functions of D-DT and DDTL are still unclear. Targeting MIF therapeutically should be carefully considered due to its multiple functions.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
Pengpeng Xia, Yunping Wu, Siqi Lian, Li Yan, Xia Meng, Qiangde Duan, Guoqiang Zhu
Summary: This paper summarizes the specificity of TLRs in recognizing microbial components, discusses their role in regulating immune responses, and proposes a novel therapeutic strategy using TLRs to combat microbial infections.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Tatsuo Kawai, Katherine J. Elliott, Rosario Scalia, Satoru Eguchi
Summary: ADAMs play a key role in cell signaling by shedding cytokines and growth factors, leading to cell proliferation and inflammation. Among them, ADAM17 is the most extensively studied member and may have significant implications in cardiovascular diseases.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Multidisciplinary Sciences
Azita Leavitt, Erez Yirmiya, Gil Amitai, Allen Lu, Jeremy Garb, Ehud Herbst, Benjamin R. Morehouse, Samuel J. Hobbs, Sadie P. Antine, Zhen-Yu J. Sun, Philip J. Kranzusch, Rotem Sorek
Summary: The Toll/interleukin-1 receptor (TIR) domain is a crucial component of immune receptors that detect pathogen invasion in various organisms. This study reveals a new mode of action by which phages inhibit host immunity by binding and sequestering immune signaling molecules produced by TIR-domain proteins. The chemical structure of the central immune signaling molecule has been defined, providing insights into the mechanisms of pathogen-host interactions.
Article
Pharmacology & Pharmacy
Seungkirl Ahn, Biswaranjan Pani, Alem W. Kahsai, Eva K. Olsen, Gitte Husemoen, Mikkel Vestergaard, Lei Jin, Shuai Zhao, Laura M. Wingler, Paula K. Rambarat, Rishabh K. Simhal, Thomas T. Xu, Lillian D. Sun, Paul J. Shim, Dean P. Staus, Li-Yin Huang, Thomas Franch, Xin Chen, Robert J. Lefkowitz
MOLECULAR PHARMACOLOGY
(2018)
Article
Multidisciplinary Sciences
Dean P. Staus, Laura M. Wingler, Minjung Choi, Biswaranjan Pani, Aashish Manglik, Andrew C. Kruse, Robert J. Lefkowitz
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Biochemistry & Molecular Biology
Minjung Choi, Dean P. Staus, Laura M. Wingler, Seungkirl Ahn, Biswaranjan Pani, William D. Capel, Robert J. Lefkowitz
Article
Biochemistry & Molecular Biology
Laura M. Wingler, Matthias Elgeti, Daniel Hilger, Naomi R. Latorraca, Michael T. Lerch, Dean P. Staus, Ron O. Dror, Brian K. Kobilka, Wayne L. Hubbell, Robert J. Lefkowitz
Article
Biochemistry & Molecular Biology
Laura M. Wingler, Conor McMahon, Dean P. Staus, Robert J. Lefkowitz, Andrew C. Kruse
Article
Biochemistry & Molecular Biology
Dean P. Staus, Laura M. Wingler, Dmitry Pichugin, Robert Scott Prosser, Robert J. Lefkowitz
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Multidisciplinary Sciences
Dean P. Staus, Hongli Hu, Michael J. Robertson, Alissa L. W. Kleinhenz, Laura M. Wingler, William D. Capel, Naomi R. Latorraca, Robert J. Lefkowitz, Georgios Skiniotis
Article
Multidisciplinary Sciences
Carl-Mikael Suomivuori, Naomi R. Latorraca, Laura M. Wingler, Stephan Eismann, Matthew C. King, Alissa L. W. Kleinhenz, Meredith A. Skiba, Dean P. Staus, Andrew C. Kruse, Robert J. Lefkowitz, Ron O. Dror
Article
Multidisciplinary Sciences
Laura M. Wingler, Meredith A. Skiba, Conor McMahon, Dean P. Staus, Alissa L. W. Kleinhenz, Carl-Mikael Suomivuori, Naomi R. Latorraca, Ron O. Dror, Robert J. Lefkowitz, Andrew C. Kruse
Meeting Abstract
Biochemistry & Molecular Biology
Conor McMahon, Laura Wingler, Dean Staus, Robert Lefkowitz, Andrew Kruse
Article
Multidisciplinary Sciences
Conor McMahon, Dean P. Staus, Laura M. Wingler, Jialu Wang, Meredith A. Skiba, Matthias Elgeti, Wayne L. Hubbell, Howard A. Rockman, Andrew C. Kruse, Robert J. Lefkowitz
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Review
Cell Biology
Laura M. Wingler, Robert J. Lefkowitz
TRENDS IN CELL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Alon Wellner, Conor McMahon, Morgan S. A. Gilman, Jonathan R. Clements, Sarah Clark, Kianna M. Nguyen, Ming H. Ho, Vincent J. Hu, Jung-Eun Shin, Jared Feldman, Blake M. Hauser, Timothy M. Caradonna, Laura M. Wingler, Aaron G. Schmidt, Debora S. Marks, Jonathan Abraham, Andrew C. Kruse, Chang C. Liu
Summary: AHEAD is a synthetic recombinant antibody generation technology that mimics somatic hypermutation inside engineered yeast, enabling the continuous mutation of antibody repertoires through simple cycles of yeast culturing and enrichment for antigen binding to produce high-affinity clones in a short timeframe.
NATURE CHEMICAL BIOLOGY
(2021)
Review
Cardiac & Cardiovascular Systems
Laura M. Wingler, Andrew P. Feld
Summary: Understanding the activation of G protein-coupled receptors (GPCRs) is crucial in cardiovascular medicine. Nanobodies, recombinant single-domain antibody fragments, have become important tools to study GPCRs in purified systems and living cells. The use of nanobodies has revealed the characteristics of GPCRs in terms of their structure, pharmacology, and signaling, and has the potential to have a significant impact in both basic science and therapeutic applications.
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
(2022)
