Rapid generation of potent antibodies by autonomous hypermutation in yeast

Introduction of the South African SARS-CoV-2 variant 501Y.V2 into the UK

(2021) Julian W. Tang et al.   JOURNAL OF INFECTION

Transmission, infectivity, and neutralization of a spike L452R SARS-CoV-2 variant

(2021) Xianding Deng et al.   CELL

Protein design and variant prediction using autoregressive generative models

(2021) Jung-Eun Shin et al.   Nature Communications

Systems for in vivo hypermutation: a quest for scale and depth in directed evolution

(2021) Gordon Rix et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Angiotensin and biased analogs induce structurally distinct active conformations within a GPCR

(2020) Laura M. Wingler et al.   SCIENCE

Structural basis for the recognition of the SARS-CoV-2 by full-length human ACE2

(2020) Renhong Yan et al.   SCIENCE

Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor

(2020) Jun Lan et al.   NATURE

Deep mutational scanning of SARS-CoV-2 receptor binding domain reveals constraints on folding and ACE2 binding

(2020) Tyler N. Starr et al.   CELL

Studies in humanized mice and convalescent humans yield a SARS-CoV-2 antibody cocktail

(2020) Johanna Hansen et al.   SCIENCE

Animal-free alternatives and the antibody iceberg

(2020) Alison Gray et al.   NATURE BIOTECHNOLOGY

‘Provocative results’ boost hopes of antibody treatment for COVID-19

(2020) Jon Cohen   SCIENCE

An alpaca nanobody neutralizes SARS-CoV-2 by blocking receptor interaction

(2020) Leo Hanke et al.   Nature Communications

Persistence and decay of human antibody responses to the receptor binding domain of SARS-CoV-2 spike protein in COVID-19 patients

(2020) Anita S. Iyer et al.   Science Immunology

Complete Mapping of Mutations to the SARS-CoV-2 Spike Receptor-Binding Domain that Escape Antibody Recognition

(2020) Allison J. Greaney et al.   Cell Host & Microbe

Slaying SARS-CoV-2 One (Single-domain) Antibody at a Time

(2020) Timothy F. Czajka et al.   TRENDS IN MICROBIOLOGY

Distinctive Activation Mechanism for Angiotensin Receptor Revealed by a Synthetic Nanobody

(2019) Laura M. Wingler et al.   CELL

Yeast surface display platform for rapid discovery of conformationally selective nanobodies

(2018) Conor McMahon et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Yeast surface display platform for rapid discovery of conformationally selective nanobodies

(2018) Conor McMahon et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Role of framework mutations and antibody flexibility in the evolution of broadly neutralizing antibodies

(2018) Victor Ovchinnikov et al.   eLife

Insights into the Structural Basis of Antibody Affinity Maturation from Next-Generation Sequencing

(2018) Arjun K. Mishra et al.   Frontiers in Immunology

Structural Basis of Epitope Recognition by Heavy-Chain Camelid Antibodies

(2018) Uroš Zavrtanik et al.   JOURNAL OF MOLECULAR BIOLOGY

Continuous directed evolution of proteins with improved soluble expression

(2018) Tina Wang et al.   Nature Chemical Biology

Scalable, Continuous Evolution of Genes at Mutation Rates above Genomic Error Thresholds

(2018) Arjun Ravikumar et al.   CELL

Tunable Expression Systems for Orthogonal DNA Replication

(2018) Ziwei Zhong et al.   ACS Synthetic Biology

Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells

(2016) Gaelen T Hess et al.   NATURE METHODS

Animal-based antibodies: Obsolete

(2016) A. C. Gray et al.   SCIENCE

Reproducibility crisis: Blame it on the antibodies

(2015) Monya Baker   NATURE

Overlapping hotspots in CDRs are critical sites for V region diversification

(2015) Lirong Wei et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly

(2015) Michael E. Lee et al.   ACS Synthetic Biology

iDISCO: A Simple, Rapid Method to Immunolabel Large Tissue Samples for Volume Imaging

(2014) Nicolas Renier et al.   CELL

An orthogonal DNA replication system in yeast

(2014) Arjun Ravikumar et al.   Nature Chemical Biology

A robust pipeline for rapid production of versatile nanobody repertoires

(2014) Peter C Fridy et al.   NATURE METHODS

Nanobodies: Natural Single-Domain Antibodies

(2013) Serge Muyldermans   Annual Review of Biochemistry

Structural and molecular interrogation of intact biological systems

(2013) Kwanghun Chung et al.   NATURE

Engineering antibodies by yeast display

(2012) Eric T. Boder et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription

(2011) Feng Zhang et al.   NATURE BIOTECHNOLOGY

Directed evolution of a secretory leader for the improved expression of heterologous proteins and full-length antibodies inSaccharomyces cerevisiae

(2009) J. Andy Rakestraw et al.   BIOTECHNOLOGY AND BIOENGINEERING

Antibody-Based Sensors: Principles, Problems and Potential for Detection of Pathogens and Associated Toxins

(2009) Barry Byrne et al.   SENSORS