Clarification technologies for monoclonal antibody manufacturing processes: Current state and future perspectives

Development and scale-up of the recovery and purification of a domain antibody Fc fusion protein-comparison of a two and three-step approach

(2015) Sibylle Herzer et al.   BIOTECHNOLOGY AND BIOENGINEERING

Continuous precipitation of IgG from CHO cell culture supernatant in a tubular reactor

(2015) Nikolaus Hammerschmidt et al.   Biotechnology Journal

Mechanistic evaluation of virus clearance by depth filtration

(2015) Adith Venkiteshwaran et al.   BIOTECHNOLOGY PROGRESS

Multistage aqueous two-phase extraction of a monoclonal antibody from cell supernatant

(2015) Jan Muendges et al.   BIOTECHNOLOGY PROGRESS

Single stage aqueous two-phase extraction for monoclonal antibody purification from cell supernatant

(2015) Jan Muendges et al.   FLUID PHASE EQUILIBRIA

PDADMAC flocculation of Chinese hamster ovary cells: Enabling a centrifuge-less harvest process for monoclonal antibodies

(2015) Thomas McNerney et al.   mAbs

Complete clarification solution for processing high density cell culture harvests

(2015) Sladjana Tomic et al.   SEPARATION AND PURIFICATION TECHNOLOGY

Continuous precipitation of IgG from CHO cell culture supernatant in a tubular reactor

(2015) Nikolaus Hammerschmidt et al.   Biotechnology Journal

Affinity precipitation of a monoclonal antibody from an industrial harvest feedstock using an ELP-Z stimuli responsive biopolymer

(2014) Rahul D. Sheth et al.   BIOTECHNOLOGY AND BIOENGINEERING

A novel approach to monitor clearance of host cell proteins associated with monoclonal antibodies

(2014) Nabila Aboulaich et al.   BIOTECHNOLOGY PROGRESS

Ethanol precipitation for purification of recombinant antibodies

(2014) Anne Tscheliessnig et al.   JOURNAL OF BIOTECHNOLOGY

Nonspecific interactions of chromatin with immunoglobulin G and protein A, and their impact on purification performance

(2014) Pete Gagnon et al.   JOURNAL OF CHROMATOGRAPHY A

Coagulation–flocculation mechanisms in wastewater treatment plants through zeta potential measurements

(2014) E.A. López-Maldonado et al.   JOURNAL OF HAZARDOUS MATERIALS

Treatment of highly concentrated wastewater containing multiple synthetic dyes by a combined process of coagulation/flocculation and nanofiltration

(2014) Can-Zeng Liang et al.   JOURNAL OF MEMBRANE SCIENCE

Comprehensive tracking of host cell proteins during monoclonal antibody purifications using mass spectrometry

(2014) Qingchun Zhang et al.   mAbs

Combined polyethylene glycol and CaCl2 precipitation for the capture and purification of recombinant antibodies

(2014) Ralf Sommer et al.   PROCESS BIOCHEMISTRY

Downstream processing of biopharmaceutical proteins produced in plants

(2014) Johannes Felix Buyel et al.   Bioengineered

Feasibility study of semi-selective protein precipitation with salt-tolerant copolymers for industrial purification of therapeutic antibodies

(2013) Florian Capito et al.   BIOTECHNOLOGY AND BIOENGINEERING

High-throughput screening for the development of a monoclonal antibody affinity precipitation step using ELP-z stimuli responsive biopolymers

(2013) Rahul D. Sheth et al.   BIOTECHNOLOGY AND BIOENGINEERING

Identification and characterization of host cell protein product-associated impurities in monoclonal antibody bioprocessing

(2013) Nicholas E. Levy et al.   BIOTECHNOLOGY AND BIOENGINEERING

Clarification of recombinant proteins from high cell density mammalian cell culture systems using new improved depth filters

(2013) Nripen Singh et al.   BIOTECHNOLOGY AND BIOENGINEERING

Polyelectrolyte–protein interaction at low ionic strength: required chain flexibility depending on protein average charge

(2013) Florian Capito et al.   COLLOID AND POLYMER SCIENCE

Purification of antibodies by precipitating impurities using Polyethylene Glycol to enable a two chromatography step process

(2013) Glen Giese et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Caprylic acid precipitation method for impurity reduction: An alternative to conventional chromatography for monoclonal antibody purification

(2012) Yan Brodsky et al.   BIOTECHNOLOGY AND BIOENGINEERING

The dynamics of the CHO host cell protein profile during clarification and protein A capture in a platform antibody purification process

(2012) Catherine E.M. Hogwood et al.   BIOTECHNOLOGY AND BIOENGINEERING

Host cell protein dynamics in the supernatant of a mAb producing CHO cell line

(2011) A.S. Tait et al.   BIOTECHNOLOGY AND BIOENGINEERING

Select host cell proteins coelute with monoclonal antibodies in protein a chromatography

(2011) Bartek Nogal et al.   BIOTECHNOLOGY PROGRESS

Mechanistic insights into protein precipitation by alcohol

(2011) Hiroki Yoshikawa et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Antibody capture by mixed-mode chromatography: A comprehensive study from determination of optimal purification conditions to identification of contaminating host cell proteins

(2011) Jerome Pezzini et al.   JOURNAL OF CHROMATOGRAPHY A

Effects of solution environment on mammalian cell fermentation broth properties: Enhanced impurity removal and clarification performance

(2010) Matthew Westoby et al.   BIOTECHNOLOGY AND BIOENGINEERING

Downstream antibody purification using aqueous two-phase extraction

(2010) Lisong Nathan Mao et al.   BIOTECHNOLOGY PROGRESS

Using precipitation by polyamines as an alternative to chromatographic separation in antibody purification processes

(2010) Junfen Ma et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Ribonuclease S-peptide as a carrier in fusion proteins

(2010) Jin-Soo Kim et al.   PROTEIN SCIENCE

Recent advances in large-scale production of monoclonal antibodies and related proteins

(2010) Abhinav A. Shukla et al.   TRENDS IN BIOTECHNOLOGY

Entering and Exiting the Protein−Polyelectrolyte Coacervate Phase via Nonmonotonic Salt Dependence of Critical Conditions

(2009) Margarita Antonov et al.   BIOMACROMOLECULES

Design of a filter train for precipitate removal in monoclonal antibody downstream processing

(2009) Sunitha Kandula et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Design of salt-tolerant membrane adsorbers for viral clearance

(2009) William Riordan et al.   BIOTECHNOLOGY AND BIOENGINEERING

Profiling of host cell proteins by two-dimensional difference gel electrophoresis (2D-DIGE): Implications for downstream process development

(2009) Mi Jin et al.   BIOTECHNOLOGY AND BIOENGINEERING

Application of aqueous two-phase systems to antibody purification: A multi-stage approach

(2009) P.A.J. Rosa et al.   JOURNAL OF BIOTECHNOLOGY

Roadmap for implementation of quality by design (QbD) for biotechnology products

(2009) Anurag S. Rathore   TRENDS IN BIOTECHNOLOGY

Viral clearance using disposable systems in monoclonal antibody commercial downstream processing

(2008) Joe X. Zhou et al.   BIOTECHNOLOGY AND BIOENGINEERING

Selective antibody precipitation using polyelectrolytes: A novel approach to the purification of monoclonal antibodies

(2008) Paul McDonald et al.   BIOTECHNOLOGY AND BIOENGINEERING

Advances in Primary Recovery: Centrifugation and Membrane Technology

(2008) D.J. Roush et al.   BIOTECHNOLOGY PROGRESS

Bioseparation in Antibody Manufacturing: The Good, The Bad and The Ugly

(2008) U. Gottschalk   BIOTECHNOLOGY PROGRESS

Settling rates for flocculation of iron and manganese ore-containing suspensions by cationic glycogen

(2008) Witold Brostow et al.   POLYMER ENGINEERING AND SCIENCE

Purification of plasmid DNA with aqueous two phase systems of PEG 600 and sodium citrate/ammonium sulfate

(2008) Gabriela A. Gomes et al.   SEPARATION AND PURIFICATION TECHNOLOGY

Partitioning of human antibodies in polyethylene glycol–sodium citrate aqueous two-phase systems

(2007) Ana M. Azevedo et al.   SEPARATION AND PURIFICATION TECHNOLOGY