The CRISPR toolbox in medical mycology: State of the art and perspectives

Plasmid-Based CRISPR-Cas9 Gene Editing in Multiple Candida Species

(2019) Lisa Lombardi et al.   mSphere

Implementation of a CRISPR-Based System for Gene Regulation in Candida albicans

(2019) Elvira Román et al.   mSphere

A CRISPR Interference Platform for Efficient Genetic Repression in Candida albicans

(2019) Lauren Wensing et al.   mSphere

Finding the needle in a haystack: Mapping antifungal drug resistance in fungal pathogen by genomic approaches

(2019) Dominique Sanglard   PLoS Pathogens

Design, execution, and analysis of CRISPR–Cas9-based deletions and genetic interaction networks in the fungal pathogen Candida albicans

(2019) Viola Halder et al.   Nature Protocols

Abrogation of Triazole Resistance upon Deletion of CDR1 in a Clinical Isolate of Candida auris

(2019) Jeffrey M. Rybak et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

CRISPR/Cas9-mediated endogenous gene tagging in Fusarium oxysporum

(2019) Qiang Wang et al.   FUNGAL GENETICS AND BIOLOGY

Recreation of in-host acquired single nucleotide polymorphisms by CRISPR-Cas9 reveals an uncharacterised gene playing a role in Aspergillus fumigatus azole resistance via a non-cyp51A mediated resistance mechanism

(2019) Eloise Ballard et al.   FUNGAL GENETICS AND BIOLOGY

Precise genome editing using a CRISPR-Cas9 method highlights the role of CoERG11 amino acid substitutions in azole resistance in Candida orthopsilosis

(2019) Florent Morio et al.   JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

Aneuploidy Enables Cross-Adaptation to Unrelated Drugs

(2019) Feng Yang et al.   MOLECULAR BIOLOGY AND EVOLUTION

FLASH: a next-generation CRISPR diagnostic for multiplexed detection of antimicrobial resistance sequences

(2019) Jenai Quan et al.   NUCLEIC ACIDS RESEARCH

Circuit diversification in a biofilm regulatory network

(2019) Manning Y. Huang et al.   PLoS Pathogens

Advancing Functional Genetics Through Agrobacterium-Mediated Insertional Mutagenesis and CRISPR/Cas9 in the Commensal and Pathogenic Yeast Malassezia

(2019) Giuseppe Ianiri et al.   GENETICS

Creating functional chromosome fusions in yeast with CRISPR–Cas9

(2019) Yangyang Shao et al.   Nature Protocols

Correlating Genotype and Phenotype in the Asexual Yeast Candida orthopsilosis Implicates ZCF29 in Sensitivity to Caffeine

(2019) Kontxi Martinez de San Vicente et al.   G3-Genes Genomes Genetics

Genomes in Focus: Development and Applications of CRISPR-Cas9 Imaging Technologies

(2018) Spencer C. Knight et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Cas9/CRISPR genome editing to demonstrate the contribution of Cyp51A Gly138Ser to azole resistance in Aspergillus fumigatus

(2018) Takashi Umeyama et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts: Current state and future prospects

(2018) Hana Raschmanová et al.   BIOTECHNOLOGY ADVANCES

Multiple Applications of a Transient CRISPR-Cas9 Coupled with Electroporation (TRACE) System in theCryptococcus neoformansSpecies Complex

(2018) Yumeng Fan et al.   GENETICS

A standardized toolkit for genetic engineering of CTG clade yeasts

(2018) Tatiana A. Defosse et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Highly parallel genome variant engineering with CRISPR–Cas9

(2018) Meru J. Sadhu et al.   NATURE GENETICS

Field-deployable viral diagnostics using CRISPR-Cas13

(2018) Cameron Myhrvold et al.   SCIENCE

Worldwide emergence of resistance to antifungal drugs challenges human health and food security

(2018) Matthew C. Fisher et al.   SCIENCE

CRISPR/Cas9-Mediated Gene Disruption Reveals the Importance of Zinc Metabolism for Fitness of the Dimorphic Fungal Pathogen Blastomyces dermatitidis

(2018) Gregory C. Kujoth et al.   mBio

The CRISPR tool kit for genome editing and beyond

(2018) Mazhar Adli   Nature Communications

Systematic Complex Haploinsufficiency-Based Genetic Analysis of Candida albicans Transcription Factors: Tools and Applications to Virulence-Associated Phenotypes

(2018) Virginia E. Glazier et al.   G3-Genes Genomes Genetics

Multiple Applications of a Transient CRISPR-Cas9 Coupled with Electroporation (TRACE) System in theCryptococcus neoformansSpecies Complex

(2018) Yumeng Fan et al.   GENETICS

Two Distinct Approaches for CRISPR-Cas9-Mediated Gene Editing in Cryptococcus neoformans and Related Species

(2018) Ping Wang et al.   mSphere

Yeast genetic interaction screens in the age of CRISPR/Cas

(2018) Neil R. Adames et al.   CURRENT GENETICS

CORT0C04210 is required for Candida orthopsilosis adhesion to human buccal cells

(2018) Marina Zoppo et al.   FUNGAL GENETICS AND BIOLOGY

CRISPR/Cpf1 facilitated large fragment deletion in Saccharomyces cerevisiae

(2018) Zhen-Hai Li et al.   JOURNAL OF BASIC MICROBIOLOGY

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window

(2018) Shakked O. Halperin et al.   NATURE

CRISPR-Cas guides the future of genetic engineering

(2018) Gavin J. Knott et al.   SCIENCE

Allele-specific genome editing using CRISPR–Cas9 is associated with loss of heterozygosity in diploid yeast

(2018) Arthur R Gorter de Vries et al.   NUCLEIC ACIDS RESEARCH

Gene Essentiality Analyzed by In Vivo Transposon Mutagenesis and Machine Learning in a Stable Haploid Isolate of Candida albicans

(2018) Ella Shtifman Segal et al.   mBio

CRISPR-Cas9 induces point mutation in the mucormycosis fungus Rhizopus delemar

(2018) Gillian O. Bruni et al.   FUNGAL GENETICS AND BIOLOGY

Improvement of fungal disease identification and management: combined health systems and public health approaches

(2017) Donald C Cole et al.   LANCET INFECTIOUS DISEASES

Enhanced proofreading governs CRISPR–Cas9 targeting accuracy

(2017) Janice S. Chen et al.   NATURE

RNA targeting with CRISPR–Cas13

(2017) Omar O. Abudayyeh et al.   NATURE

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

(2017) Nicole M. Gaudelli et al.   NATURE

Gene editing in clinical isolates of Candida parapsilosis using CRISPR/Cas9

(2017) Lisa Lombardi et al.   Scientific Reports

Location, location, location: Use of CRISPR-Cas9 for genome editing in human pathogenic fungi

(2017) Aaron P. Mitchell   PLoS Pathogens

Candida auris: A rapidly emerging cause of hospital-acquired multidrug-resistant fungal infections globally

(2017) Anuradha Chowdhary et al.   PLoS Pathogens

Antifungal Therapy: New Advances in the Understanding and Treatment of Mycosis

(2017) Liliana Scorzoni et al.   Frontiers in Microbiology

A CRISPR–Cas9-based gene drive platform for genetic interaction analysis in Candida albicans

(2017) Rebecca S. Shapiro et al.   Nature Microbiology

An Efficient, Rapid, and Recyclable System for CRISPR-Mediated Genome Editing in Candida albicans

(2017) Namkha Nguyen et al.   mSphere

Marker Recycling in Candida albicans through CRISPR-Cas9-Induced Marker Excision

(2017) Manning Y. Huang et al.   mSphere

Development of a CRISPR-Cas9 System for Efficient Genome Editing of Candida lusitaniae

(2017) Emily L. Norton et al.   mSphere

Use of RNA-Protein Complexes for Genome Editing in Non- albicans Candida Species

(2017) Nora Grahl et al.   mSphere

Development of a plasmid free CRISPR-Cas9 system for the genetic modification of Mucor circinelloides

(2017) Gábor Nagy et al.   Scientific Reports

Large fragment deletion using a CRISPR/Cas9 system in Saccharomyces cerevisiae

(2016) Huanhuan Hao et al.   ANALYTICAL BIOCHEMISTRY

Highly efficient CRISPR mutagenesis by microhomology-mediated end joining in Aspergillus fumigatus

(2016) Chi Zhang et al.   FUNGAL GENETICS AND BIOLOGY

CRISPR-Cas9, the new kid on the block of fungal molecular biology

(2016) Sven Krappmann   MEDICAL MYCOLOGY

Efficient introduction of specific homozygous and heterozygous mutations using CRISPR/Cas9

(2016) Dominik Paquet et al.   NATURE

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Repair Pathway Choices and Consequences at the Double-Strand Break

(2016) Raphael Ceccaldi et al.   TRENDS IN CELL BIOLOGY

Systematic functional analysis of kinases in the fungal pathogen Cryptococcus neoformans

(2016) Kyung-Tae Lee et al.   Nature Communications

Genome engineering in the yeast pathogen Candida glabrata using the CRISPR-Cas9 system

(2016) Ludovic Enkler et al.   Scientific Reports

Functional Reconstitution of a Fungal Natural Product Gene Cluster by Advanced Genome Editing

(2016) Jakob Weber et al.   ACS Synthetic Biology

Targeted Genome Editing via CRISPR in the Pathogen Cryptococcus neoformans

(2016) Samantha D. M. Arras et al.   PLoS One

A ‘suicide’ CRISPR-Cas9 system to promote gene deletion and restoration by electroporation in Cryptococcus neoformans

(2016) Yu Wang et al.   Scientific Reports

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Development of the CRISPR/Cas9 System for Targeted Gene Disruption in Aspergillus fumigatus

(2015) Kevin K. Fuller et al.   EUKARYOTIC CELL

Recognition of seven species in the Cryptococcus gattii/Cryptococcus neoformans species complex

(2015) Ferry Hagen et al.   FUNGAL GENETICS AND BIOLOGY

Safeguarding CRISPR-Cas9 gene drives in yeast

(2015) James E DiCarlo et al.   NATURE BIOTECHNOLOGY

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

The evolution of drug resistance in clinical isolates of Candida albicans

(2015) Christopher B Ford et al.   eLife

A Candida albicans CRISPR system permits genetic engineering of essential genes and gene families

(2015) V. K. Vyas et al.   Science Advances

Generation of stable mutants and targeted gene deletion strains in Cryptococcus neoformans through electroporation

(2014) Xiaorong Lin et al.   MEDICAL MYCOLOGY

The Cryptococcus neoformans Transcriptome at the Site of Human Meningitis

(2014) Y. Chen et al.   mBio

Comparative Phenotypic Analysis of the Major Fungal Pathogens Candida parapsilosis and Candida albicans

(2014) Linda M. Holland et al.   PLoS Pathogens

Systematic Phenotyping of a Large-Scale Candida glabrata Deletion Collection Reveals Novel Antifungal Tolerance Genes

(2014) Tobias Schwarzmüller et al.   PLoS Pathogens

Self-processing of ribozyme-flanked RNAs into guide RNAsin vitroandin vivofor CRISPR-mediated genome editing

(2013) Yangbin Gao et al.   Journal of Integrative Plant Biology

The ‘obligate diploid’ Candida albicans forms mating-competent haploids

(2013) Meleah A. Hickman et al.   NATURE

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

(2013) James E. DiCarlo et al.   NUCLEIC ACIDS RESEARCH

Metabolic engineering of Rhizopus oryzae for the production of platform chemicals

(2012) Bas J. Meussen et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Milestones in Candida albicans gene manipulation

(2011) Dhanushki P. Samaranayake et al.   FUNGAL GENETICS AND BIOLOGY

Systematic screens of a Candida albicans homozygous deletion library decouple morphogenetic switching and pathogenicity

(2010) Suzanne M Noble et al.   NATURE GENETICS

Single-strand break repair and genetic disease

(2008) Keith W. Caldecott   NATURE REVIEWS GENETICS