In vitro models of neuromuscular junctions and their potential for novel drug discovery and development

A 3D culture model of innervated human skeletal muscle enables studies of the adult neuromuscular junction

(2019) Mohsen Afshar Bakooshli et al.   eLife

Stem cell derived phenotypic human neuromuscular junction model for dose response evaluation of therapeutics

(2018) Navaneetha Santhanam et al.   BIOMATERIALS

Organ/body-on-a-chip based on microfluidic technology for drug discovery

(2018) Hiroshi Kimura et al.   Drug Metabolism and Pharmacokinetics

Animal Models of the Neuromuscular Junction, Vitally Informative for Understanding Function and the Molecular Mechanisms of Congenital Myasthenic Syndromes

(2018) Richard Webster   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Rapamycin treatment for amyotrophic lateral sclerosis

(2018) Jessica Mandrioli et al.   MEDICINE

Advanced maturation of human cardiac tissue grown from pluripotent stem cells

(2018) Kacey Ronaldson-Bouchard et al.   NATURE

Engineering human pluripotent stem cells into a functional skeletal muscle tissue

(2018) Lingjun Rao et al.   Nature Communications

Microphysiological 3D model of amyotrophic lateral sclerosis (ALS) from human iPS-derived muscle cells and optogenetic motor neurons

(2018) Tatsuya Osaki et al.   Science Advances

Mechanically patterned neuromuscular junctions-in-a-dish have improved functional maturation

(2017) Cassandra L. Happe et al.   MOLECULAR BIOLOGY OF THE CELL

Cellular and Molecular Anatomy of the Human Neuromuscular Junction

(2017) Ross A. Jones et al.   Cell Reports

Functional Connectivity under Optogenetic Control Allows Modeling of Human Neuromuscular Disease

(2016) Julius A. Steinbeck et al.   Cell Stem Cell

Creating Interactions between Tissue-Engineered Skeletal Muscle and the Peripheral Nervous System

(2016) Alec S.T. Smith et al.   CELLS TISSUES ORGANS

Modeling ALS with motor neurons derived from human induced pluripotent stem cells

(2016) Samuel Sances et al.   NATURE NEUROSCIENCE

Microfluidic device for the formation of optically excitable, three-dimensional, compartmentalized motor units

(2016) S. G. M. Uzel et al.   Science Advances

A compartmentalized microfluidic neuromuscular co-culture system reveals spatial aspects of GDNF functions

(2015) E. E. Zahavi et al.   JOURNAL OF CELL SCIENCE

Formation and characterisation of neuromuscular junctions between hiPSC derived motoneurons and myotubes

(2015) M. Demestre et al.   Stem Cell Research

Human iPSC-derived motoneurons harbouring TARDBP or C9ORF72 ALS mutations are dysfunctional despite maintaining viability

(2015) Anna-Claire Devlin et al.   Nature Communications

Generation and expansion of highly pure motor neuron progenitors from human pluripotent stem cells

(2015) Zhong-Wei Du et al.   Nature Communications

Bioengineered human myobundles mimic clinical responses of skeletal muscle to drugs

(2015) Lauran Madden et al.   eLife

Modeling the Early Phenotype at the Neuromuscular Junction of Spinal Muscular Atrophy Using Patient-Derived iPSCs

(2015) Michiko Yoshida et al.   Stem Cell Reports

Pathways Disrupted in Human ALS Motor Neurons Identified through Genetic Correction of Mutant SOD1

(2014) Evangelos Kiskinis et al.   Cell Stem Cell

Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes

(2014) Yves Maury et al.   NATURE BIOTECHNOLOGY

Chemically defined generation of human cardiomyocytes

(2014) Paul W Burridge et al.   NATURE METHODS

A Stem-Cell Based Bioassay to Critically Assess the Pathology of Dysfunctional Neuromuscular Junctions

(2014) Peter H. Chipman et al.   PLoS One

The Neuromuscular Junction: Aging at the Crossroad between Nerves and Muscle

(2014) Marta Gonzalez-Freire et al.   Frontiers in Aging Neuroscience

ALS as a distal axonopathy: molecular mechanisms affecting neuromuscular junction stability in the presymptomatic stages of the disease

(2014) Elizabeth B. Moloney et al.   Frontiers in Neuroscience

Three-dimensional neuron–muscle constructs with neuromuscular junctions

(2013) Yuya Morimoto et al.   BIOMATERIALS

Microfluidic primary culture model of the lower motor neuron–neuromuscular junction circuit

(2013) Katherine A. Southam et al.   JOURNAL OF NEUROSCIENCE METHODS

Astrocyte pathology and the absence of non-cell autonomy in an induced pluripotent stem cell model of TDP-43 proteinopathy

(2013) A. Serio et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Design and formulation of functional pluripotent stem cell-derived cardiac microtissues

(2013) N. Thavandiran et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Targeting RNA Foci in iPSC-Derived Motor Neurons from ALS Patients with a C9ORF72 Repeat Expansion

(2013) D. Sareen et al.   Science Translational Medicine

In vitro differentiation of functional human skeletal myotubes in a defined system

(2013) Xiufang Guo et al.   Biomaterials Science

Signaling and aging at the neuromuscular synapse: lessons learnt from neuromuscular diseases

(2012) Anna Rostedt Punga et al.   CURRENT OPINION IN PHARMACOLOGY

Inhibition of Apoptosis Blocks Human Motor Neuron Cell Death in a Stem Cell Model of Spinal Muscular Atrophy

(2012) Dhruv Sareen et al.   PLoS One

Genetic Correction of Human Induced Pluripotent Stem Cells from Patients with Spinal Muscular Atrophy

(2012) S. Corti et al.   Science Translational Medicine

Neuromuscular junction formation between human stem cell-derived motoneurons and human skeletal muscle in a defined system

(2011) Xiufang Guo et al.   BIOMATERIALS

A defined long-term in vitro tissue engineered model of neuromuscular junctions

(2010) Mainak Das et al.   BIOMATERIALS

A systematic review of population based epidemiological studies in Myasthenia Gravis

(2010) Aisling S Carr et al.   BMC Neurology

To build a synapse: signaling pathways in neuromuscular junction assembly

(2010) H. Wu et al.   DEVELOPMENT

Review: Neuromuscular synaptic vulnerability in motor neurone disease: amyotrophic lateral sclerosis and spinal muscular atrophy

(2010) L. M. Murray et al.   NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY

Neuromuscular Junction Formation Between Human Stem-Cell-Derived Motoneurons and Rat Skeletal Muscle in a Defined System

(2010) Xiufang Guo et al.   TISSUE ENGINEERING PART C-METHODS

Current challenges in the evaluation of cardiac safety during drug development: Translational medicine meets the Critical Path Initiative

(2009) Jonathan P. Piccini et al.   AMERICAN HEART JOURNAL

Highly efficient generation of human hepatocyte-like cells from induced pluripotent stem cells

(2009) Karim Si-Tayeb et al.   HEPATOLOGY

Impaired Synaptic Vesicle Release and Immaturity of Neuromuscular Junctions in Spinal Muscular Atrophy Mice

(2009) L. Kong et al.   JOURNAL OF NEUROSCIENCE

Differentiation of spinal motor neurons from pluripotent human stem cells

(2009) Bao-Yang Hu et al.   Nature Protocols

Reduced SMN protein impairs maturation of the neuromuscular junctions in mouse models of spinal muscular atrophy

(2008) Shingo Kariya et al.   HUMAN MOLECULAR GENETICS

Induced pluripotent stem cells from a spinal muscular atrophy patient

(2008) Allison D. Ebert et al.   NATURE

Highly efficient differentiation of hESCs to functional hepatic endoderm requires ActivinA and Wnt3a signaling

(2008) D. C. Hay et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Induced Pluripotent Stem Cells Generated from Patients with ALS Can Be Differentiated into Motor Neurons

(2008) J. T. Dimos et al.   SCIENCE

Effect of complement and its regulation on myasthenia gravis pathogenesis

(2007) Linda L Kusner et al.   Expert Review of Clinical Immunology